EP0370103A4 - Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products - Google Patents

Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products

Info

Publication number
EP0370103A4
EP0370103A4 EP19890906977 EP89906977A EP0370103A4 EP 0370103 A4 EP0370103 A4 EP 0370103A4 EP 19890906977 EP19890906977 EP 19890906977 EP 89906977 A EP89906977 A EP 89906977A EP 0370103 A4 EP0370103 A4 EP 0370103A4
Authority
EP
European Patent Office
Prior art keywords
deficient
protease
gram
production
positive bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19890906977
Other versions
EP0370103A1 (en
Inventor
Peter Blackburn
Michael Arthur Lonetto
Edward L Chang
June Polak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Microbiology Inc
Original Assignee
Health Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Health Research Institute filed Critical Health Research Institute
Publication of EP0370103A1 publication Critical patent/EP0370103A1/en
Publication of EP0370103A4 publication Critical patent/EP0370103A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96466—Cysteine endopeptidases (3.4.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP19890906977 1988-05-05 1989-03-14 Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products Withdrawn EP0370103A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19048388A 1988-05-05 1988-05-05
US190483 1988-05-05

Publications (2)

Publication Number Publication Date
EP0370103A1 EP0370103A1 (en) 1990-05-30
EP0370103A4 true EP0370103A4 (en) 1991-11-27

Family

ID=22701537

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19890906977 Withdrawn EP0370103A4 (en) 1988-05-05 1989-03-14 Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products

Country Status (11)

Country Link
EP (1) EP0370103A4 (en)
JP (1) JPH03500606A (en)
AU (1) AU622916B2 (en)
DK (1) DK1590A (en)
FI (1) FI900045A (en)
HU (1) HUT53154A (en)
IL (1) IL89767A0 (en)
NZ (1) NZ228424A (en)
PT (1) PT90463A (en)
WO (1) WO1989010976A1 (en)
ZA (1) ZA892325B (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5143846A (en) * 1988-03-17 1992-09-01 The General Hospital Corporation Protease deficient bacterial hosts
US5294542A (en) * 1991-03-19 1994-03-15 Omnigene, Inc. Residual protease-III
IL102259A0 (en) * 1991-07-01 1993-01-14 Amgen Inc Isolation and characterization of a protease from streptomyces lividans
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
DK0725653T3 (en) 1993-10-05 2004-10-11 Celltech Pharmaceuticals Ltd vaccine preparations
GB9324529D0 (en) * 1993-11-30 1994-01-19 Univ Singapore Biological control agents
DE4425645A1 (en) * 1994-07-20 1996-02-22 Mueller Karl & Co Kg New lysostaphin gene with deletion in pro-segment repeat region
AU8401798A (en) * 1997-07-15 1999-02-10 Genencor International B.V. Proteases from gram-positive organisms
US6762039B2 (en) * 1997-07-15 2004-07-13 Genencor International, Inc. Bacillus subtillis with an inactivated cysteine protease-1
US6465186B1 (en) 1997-12-30 2002-10-15 Genecor International, Inc. Proteases from gram positive organisms
US6528255B1 (en) 1997-12-30 2003-03-04 Genencor International, Inc. Proteases from gram positive organisms
US6599731B1 (en) 1997-12-30 2003-07-29 Genencor International, Inc. Proteases from gram positive organisms
GB9727470D0 (en) 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006264A1 (en) * 1986-04-16 1987-10-22 Public Health Research Institute Of The City Of Ne Expression of the cloned lysostaphin gene

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988006624A2 (en) * 1987-02-27 1988-09-07 Gist-Brocades N.V. Molecular cloning and expression of genes encoding proteolytic enzymes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006264A1 (en) * 1986-04-16 1987-10-22 Public Health Research Institute Of The City Of Ne Expression of the cloned lysostaphin gene

Also Published As

Publication number Publication date
PT90463A (en) 1989-11-30
AU622916B2 (en) 1992-04-30
JPH03500606A (en) 1991-02-14
HUT53154A (en) 1990-09-28
EP0370103A1 (en) 1990-05-30
DK1590D0 (en) 1990-01-04
IL89767A0 (en) 1989-09-28
ZA892325B (en) 1990-03-28
FI900045A0 (en) 1990-01-04
FI900045A (en) 1990-01-04
DK1590A (en) 1990-02-05
WO1989010976A1 (en) 1989-11-16
HU894054D0 (en) 1990-07-28
NZ228424A (en) 1992-06-25
AU3765189A (en) 1989-11-29

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 19900104

AK Designated contracting states

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A4 Supplementary search report drawn up and despatched

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DA4 Supplementary search report drawn up and despatched (deleted)
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Effective date: 19920421

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: APPLIED MICROBIOLOGY, INC.

17Q First examination report despatched

Effective date: 19931021

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 19940503