EP0329736A4 - Asexual induction of heritable male sterility and apomixis in plants - Google Patents
Asexual induction of heritable male sterility and apomixis in plantsInfo
- Publication number
- EP0329736A4 EP0329736A4 EP19880906709 EP88906709A EP0329736A4 EP 0329736 A4 EP0329736 A4 EP 0329736A4 EP 19880906709 EP19880906709 EP 19880906709 EP 88906709 A EP88906709 A EP 88906709A EP 0329736 A4 EP0329736 A4 EP 0329736A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- plant
- hybrid
- recipient
- ams
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 206010021929 Infertility male Diseases 0.000 title claims abstract 14
- 208000007466 Male Infertility Diseases 0.000 title claims abstract 14
- 230000006698 induction Effects 0.000 title abstract 2
- 241000196324 Embryophyta Species 0.000 claims abstract 145
- 238000000034 method Methods 0.000 claims abstract 63
- 239000013598 vector Substances 0.000 claims abstract 28
- 244000062793 Sorghum vulgare Species 0.000 claims abstract 19
- 239000000284 extract Substances 0.000 claims abstract 13
- 244000020551 Helianthus annuus Species 0.000 claims abstract 10
- 240000003768 Solanum lycopersicum Species 0.000 claims abstract 10
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims abstract 10
- 235000019713 millet Nutrition 0.000 claims abstract 10
- 244000068988 Glycine max Species 0.000 claims abstract 9
- 108020004707 nucleic acids Proteins 0.000 claims abstract 6
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 6
- 102000039446 nucleic acids Human genes 0.000 claims abstract 6
- 239000002245 particle Substances 0.000 claims abstract 6
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims abstract 5
- 235000010469 Glycine max Nutrition 0.000 claims abstract 4
- 235000003222 Helianthus annuus Nutrition 0.000 claims abstract 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims abstract 4
- 240000008042 Zea mays Species 0.000 claims abstract 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract 4
- 235000005822 corn Nutrition 0.000 claims abstract 4
- 241000219823 Medicago Species 0.000 claims 17
- 230000001939 inductive effect Effects 0.000 claims 11
- 241001057636 Dracaena deremensis Species 0.000 claims 9
- 240000007594 Oryza sativa Species 0.000 claims 7
- 241000209140 Triticum Species 0.000 claims 7
- 230000008774 maternal effect Effects 0.000 claims 5
- 230000008775 paternal effect Effects 0.000 claims 5
- 240000002024 Gossypium herbaceum Species 0.000 claims 4
- 235000004341 Gossypium herbaceum Nutrition 0.000 claims 4
- 229920000742 Cotton Polymers 0.000 claims 3
- 241000219146 Gossypium Species 0.000 claims 3
- 235000007164 Oryza sativa Nutrition 0.000 claims 3
- 235000021307 Triticum Nutrition 0.000 claims 3
- 235000009566 rice Nutrition 0.000 claims 3
- 230000000975 bioactive effect Effects 0.000 claims 2
- 230000001086 cytosolic effect Effects 0.000 claims 2
- 238000004520 electroporation Methods 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 238000002347 injection Methods 0.000 claims 2
- 239000007924 injection Substances 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 238000005507 spraying Methods 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 231100001160 nonlethal Toxicity 0.000 claims 1
- 239000000419 plant extract Substances 0.000 claims 1
- 240000004658 Medicago sativa Species 0.000 abstract 2
- 240000006394 Sorghum bicolor Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/022—Genic fertility modification, e.g. apomixis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/022—Genic fertility modification, e.g. apomixis
- A01H1/023—Male sterility
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
- C12N15/8289—Male sterility
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to methods for asexual induction of heritable male sterility and apomixis in plants. The invention is directed to factors derivable from certain plants which, when applied to certain recipient plants, induce heritable male sterility in the recipient. Such asexually transmissible male sterility factors, termed AMS/vectors, are present in extracts of certain male sterile alfalfa plants, where they are associated with a unique 1 x 10<6> (approx.) dalton molecular weight nucleic acid and a 40-110 nanometer particle. The asexually generated male-sterile plants derived by AMS/vector treatment can be used to produce new and valuable hybrids of alfalfa, corn, soybean, sorghum, sunflower, millet, tomato, and other plants.
Description
Claims
WHAT IS CLAIMED IS:
1. An AMS/vector comprising a cytoplasmic factor derived from a donor plant, which factor (a) is capable of asexually Inducing heritable male sterility in a recipient plant; (b) is subsequently derivable from the recipient plant; and (c) is present in an extract of the donor plant or recipient plant, which extract further comprises a nucleic acid of about 1 X 106 dalton molecular weight and a particle of about 40-110 nanometers.
2. The AMS/vector of claim 1 in which the donor plant is an alfalfa plant.
3. The AMS/vector of claim 2 in which the alfalfa plant has U.S.D.A. Plant Introduction No. 172429.
4. The AMS/vector of claim 2 in which the alfalfa plant has U.S.D.A. Plant Introduction No. 173733.
5. The AMS/vector of claim 2 in which the alfalfa plant has U.S.D.A. Plant Introduction No. 221469.
6. The AMS/vector of claim 2 in which the alfalfa, plant has U.S.D.A. Plant Introduction No. 223386.
7. The AMS/vector of claim 2 in which the alfalfa plant has U.S.D.A. Plant Introduction No. 243223.
8. The AMS/vector of claim 2 in which the alfalfa plant comprises plant AMS 1.29, as deposited with the ATCC and assigned accession number 40352.
9. The AMS/vector of claim 1 in which the donor plant is a corn plant.
10. The AMS/vector of claim 1 in which the donor plant is a soybean plant.
11. The AMS/vector of claim 1 in which the donor plant is a sorghum plant.
12. The AMS/vector of claim 1 in which the donor plant is a sunflower plant.
13. The AMS/vector of claim 1 in which the donor plant is a millet plant.
14. The AMS/vector of claim 1 in which the donor plant is a tomato plant.
15. A plant extract capable of inducing male sterility in a plant, comprising a non-lethal buffer and a cytoplasmic factor derived from a donor plant, which factor (a) is capable of asexually inducing heritable male sterility in a recipient plant; (b) is subsequently derivable from the recipient plant; and (c) is present in an extract of the donor plant or recipient plant, which extract further comprises a nucleic acid of about 1 X 106 dalton molecular weight and a particle of about 40-110 nanometers.
16. The extract of claim 15 in which the donor plant is an alfalfa plant.
17. The extract of claim 15 in which the donor plant is a corn plant.
18. The extract of claim 15 in which the donor plant is a soybean plant.
19. The extract of claim 15 in which the donor plant is a sorghum plant.
20. The extract of claim 15 in which the donor plant is a sunflower plant.
21. The extract of claim 15 in which the donor plant is a millet plant.
22. The extract of claim 15 in which the donor plant is a tomato plant.
23. A male-sterile plant comprising a plant that has been asexually induced to heritable male sterility by the AMS/vector of claim 1.
24. A male-sterile plant comprising a plant that has been asexually induced to heritable male sterility by the AMS/vector of claim 2.
25. A male-sterile plant comprising a plant that has been asexually induced to heritable male sterility by the AMS/vector of claim 3, 4, 5, 6, 7, or 8.
26. The plant of claim 23 which is an alfalfa plant.
27. The plant of claim 23 which is a corn plant.
28. The corn plant of claim 27 comprising B73- AMS, as deposited with the ATCC and assigned accession number 40350.
29. The corn plant of claim 27 comprising Mol7-AMS, as deposited with the ATCC and assigned accession number 40351.
30. The corn plant of claim 27 comprising A632-AMS, as deposited with the ATCC and assigned accession number 40349.
31. The plant of claim 23 which is a soybean plant.
32. The plant of claim 23 which is a sorghum plant.
33. The plant of claim 23 which is a sunflower plant.
34. The plant of claim 23 which is a millet plant.
35. The plant of claim 23 which is a tomato plant.
36. The plant of claim 23 which is a wheat plant.
37. The plant of claim 23 which is a cotton plant.
38. The plant of claim 23 which is a rice plant.
39. A progeny plant obtained by asexual propagation of the plant of claim 23, 24, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37 or 38.
40. The progeny plant of claim 39, in which the propagation is by cell culture methods.
41. The progeny plant of claim 39, in which the propagation is vegetative.
42. A seed resulting from a cross of the plant of claim 23 or 24 with a maintainer plant.
43. A seed resulting from a cross of the plant of claim 25 with a maintainer plant.
44. A seed resulting from a cross of the plant of claim 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37 or 38 with a maintainer plant.
45. A progeny plant produced by the seed of claim 42.
46. A progeny plant produced by the seed of claim 43.
47. A progeny plant produced by the seed of claim 44.
48. A method for asexually inducing male sterility in a recipient plant comprising applying the AMS/vector of claim 1 to such recipient plant.
49. A method for asexually inducing male sterility in a recipient plant comprising applying the AMS/vector of claim 2 to such recipient plant.
50. A method for asexually inducing male sterility in a recipient plant comprising applying the AMS/vector of claim 8 to such recipient plant.
51. A method for asexually inducing male sterility in a recipient plant comprising applying the extract of claim 15 to such recipient plant.
52. The method of claim 48, 49, 50 or 51 in which the application is by injection.
53. The method of claim 48, 49, 50 or 51 in which the application is by spraying.
54. The method of claim 48, 49, 50 or 51 in which the application is by use of tissue culture.
55. The method of claim 48, 49, 50 or 51 in which the application is by electroporation.
56. The method of claim 48, 49, 50 or 51 in which the recipient plant is an alfalfa plant.
57. The method of claim 48, 49, 50 or 51 in which the recipient plant is a corn plant.
58. The method of claim 48, 49, 50 or 51 in which the recipient plant is a soybean plant.
59. The method of claim 48, 49, 50 or 51 in which the recipient plant is a sorghum plant.
60. The method of claim 48, 49, 50 or 51 in which the recipient plant is a sunflower plant.
61. The method of claim 48, 49, 50 or 51 in which the recipient plant is a millet plant.
62. The method of claim 48, 49, 50 or 51 in which the recipient plant is a tomato plant.
63. The method of claim 48, 49, 50 or 51 in which the recipient plant is a wheat plant.
64. The method of claim 48, 49, 50 or 51 in which the recipient plant is a cotton plant.
65. The method of claim 48, 49, 50 and 51 in which the recipient plant is a rice plant.
66. A method for making an F1 hybrid comprising crossing a paternal parent plant with the plant of claim
23 or 24 as maternal parent.
67. A method for making an F1 hybrid comprising crossing a paternal parent plant with the plant of claim 25 as maternal parent.
68. A method for making an F1 hybrid comprising crossing a paternal parent plant with the plant of claim
45 as maternal parent.
69. A method for making an F1 hybrid comprising crossing a paternal parent plant with the plant of claim
46 as maternal parent.
70. A method for making an F1 hybrid comprising crossing a paternal parent plant with the plant of claim 47 as maternal parent.
71. An F1 hybrid made according to the method of claim 66.
72. An F1 hybrid made according to the method of claim 67.
73. An F1 hybrid made according to the method of claim 68.
74. An F1 hybrid made according to the method of claim 69.
75. An F1 hybrid made according to the method of claim 70.
76. The F1 hybrid of claim 71 which is male sterile.
77. The F1 hybrid of claim 72 which is male sterile.
78. The F1 hybrid of claim 73 which is male sterile.
79. The F1 hybrid of claim 74 which is male sterile.
80. The F1 hybrid of claim 75 which is male sterile.
81. The F1 hybrid of claim 71 which is male fertile.
82. The F1 hybrid of claim 72 which is male fertile.
83. The F1 hybrid of claim 73 which is male fertile.
84. The F1 hybrid of claim' 74 which is male fertile.
85. The F1 hybrid of claim 75 which is male fertile.
86. A seed of the F1 hybrid of claim 71.
87. A seed of the F1 hybrid of claim 72.
88. A seed of the F1 hybrid of claim 73.
89. A seed of the F1 hybrid of claim 74.
90. A seed of the F1 hybrid of claim 75.
91. A seed of the F1 hybrid of claim 76.
92. A seed of the F1 hybrid of claim 77.
93. A seed of the F1 hybrid of claim 78.
94. A seed of the F1 hybrid of claim 79.
95. A seed of the F1 hybrid of claim 80.
96 . A seed of the F1 hybrid of claim 81.
97. A seed of the F1 hybrid of claim 82.
98. A seed of the F1 hybrid of claim 83.
99. A seed of the F1 hybrid of claim 84.
100. A seed of the F1 hybrid of claim 85.
101. A method for inducing apomixis in a recipient plant comprising applying an effective amount of the AMS/vector of claim 1 to such recipient plant.
102. A method for inducing apomixis in a recipient plant comprising applying the AMS/vector of claim 2 to such recipient plant.
103. A method for inducing apomixis in a recipient plant comprising applying the AMS/vector of claim 8 to such recipient plant.
104. A method for inducing apomixis in a recipient plant comprising applying the AMS/vector of claim 15 to such recipient plant.
105. The method of claim 101, 102, 103 or 104, in which the application is by spraying.
106. The method of claim 101, 102, 103 or 104 in which the application is by injection.
107. The method of claim 101, 102, 103 or 104 in which the application is by use of tissue culture.
108. The method of claim 101, 102, 103 or 104 in which the application is by electroporation.
109. The method of claim 101, 102, 103 or 104 in which the recipient plant is an alfalfa plant.
110. The method of claim 101, 102, 103 or 104 in which the recipient plant is a corn plant.
111. The method of claim 101, 102, 103 or 104 in which the recipient plant is a sorghum plant.
112. The method of claim 101, 102, 103 or 104 in which the recipient plant is a sunflower plant.
113. The method of claim 101, 102, 103 or 104 in which the recipient plant is a millet plant.
114. The method of claim 101, 102, 103 or 104 in which the recipient plant is a tomato plant.
115. The method of claim 101, 102, 103 or 104 in which the recipient plant is a wheat plant.
116. The method of claim 101, 102, 103 or 104 in which the recipient plant is a cotton plant.
117. The method of claim 101, 102, 103 or 104 in which the recipient plant is a rice plant.
118. A method of making an apomitic hybrid which comprises treating a first parental plant line of said
hybrid with an effective amount of the AMS/vector of claim 1, and crossing said first parental line with a second parental plant line to obtain hybrid seed.
119. The method of claim 118 which includes the further step of growing the hybrid seed to produce mature plants, and identifying those plants having apomitic properties.
120. The method of claim 118 which comprises the further step of obtaining hybrid seed from the identified plants.
121. The method of claim 118, 119 or 120, wherein the plant is an alfalfa plant.
122. The method of claim 118, 119 or 120, wherein the plant is a corn plant.
123. The method of claim 118, 119 or 120, wherein the plant is a soybean plant.
124. The method of claim 118, 119 or 120, wherein the plant is a sorghum plant.
125. The method of claim 118, 119 or 120, wherein the plant is a sunflower plant.
126. The method of claim 118, 119 or 120, wherein the plant is a millet plant.
127. The method of claim 118, 119 or 120, wherein the plant is a tomato plant.
128. The method of claim 118, 119 or 120, wherein the plant is a wheat plant.
129. The method of claim 118, 119 or 120, wherein the plant is a cotton plant.
130. The method of claim 118, 119 or 120, wherein the plant is a rice plant.
131. Hybrid seed produced by the method of claim
118.
132. Hybrid seed produced by the method of claim 120.
133. A hybrid plant produced by the method of claim 119, and direct descendants thereof.
134. The seed of claim 131 wherein the plant is selected from the group consisting of corn, alfalfa, soybean, sorghum, sunflower, millet, tomato, wheat, cotton and rice.
135. The seed of claim 132 wherein the plant is selected from the group consisting of corn, alfalfa, soybean sorghum, sunflower, millet, tomato, wheat, cotton and rice.
136. The plant of claim 133 wherein the plant is selected from the group consisting of corn, alfalfa, soybean, sorghum, sunflower, millet, tomato, wheat, cotton and rice.
137. A method of delivering a bioactive molecule intracellularly to a plant comprising applying the about
40-110 nanometer particle associated with the AMS/vector of claim 1, which particle contains a bioactive molecule.
138. A plant delivery system comprising an about 40-110 nanometer particle derivable from an alfalfa plant selected from the group consisting of plants having U.S.D.A. Plant Introduction Nos. 172429, 173733, 221469, 223386, and 243223.
139. A method of expressing a heterologous gene sequence in a plant comprising applying the about 1 X 10 dalton nucleic acid of claim 1, which nucleic acid comprises a heterologous gene sequence capable of being expressed in the plant.
140. A plant expression vector comprising an about 1 X 10 dalton molecular weight nucleic acid derivable from an alfalfa plant selected from the group consisting of plants having U.S.D.A. Plant Introduction Nos. 172429, 173733, 221469, 223386, and 243223.
141. A mutant, derivative, or fragment of the expression vector of claim 108.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US224836 | 1981-01-13 | ||
| US8050587A | 1987-07-31 | 1987-07-31 | |
| US80505 | 1987-07-31 | ||
| US22483688A | 1988-07-27 | 1988-07-27 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP0329736A1 EP0329736A1 (en) | 1989-08-30 |
| EP0329736A4 true EP0329736A4 (en) | 1991-10-09 |
Family
ID=26763609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP19880906709 Withdrawn EP0329736A4 (en) | 1987-07-31 | 1988-07-28 | Asexual induction of heritable male sterility and apomixis in plants |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0329736A4 (en) |
| AU (1) | AU629796B2 (en) |
| BR (1) | BR8807155A (en) |
| DK (1) | DK155189A (en) |
| WO (1) | WO1989000810A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3931969A1 (en) * | 1989-09-25 | 1991-04-04 | Max Planck Gesellschaft | DNA SEQUENCE, WUNL GENE WITH CUT PROMOTOR AND USE THEREOF |
| US5710367A (en) * | 1995-09-22 | 1998-01-20 | The United States Of America As Represented By The Secretary Of Agriculture | Apomictic maize |
| GB9610044D0 (en) * | 1996-05-14 | 1996-07-17 | Sandoz Ltd | Improvements in or relating to organic compounds |
| US6046385A (en) * | 1997-10-09 | 2000-04-04 | Midwest Oilseeds, Inc. | Mutant male sterile gene of soybean |
| US9210847B2 (en) | 2011-05-06 | 2015-12-15 | Kenavis Corporation, Llc | Soybean varieties and methods for producing said varieties |
| CN109089874A (en) * | 2018-08-16 | 2018-12-28 | 安徽省农业科学院土壤肥料研究所 | The selection of leaf mustard cytoplasmic male sterile line, breeding system, control method |
-
1988
- 1988-07-28 BR BR888807155A patent/BR8807155A/en unknown
- 1988-07-28 EP EP19880906709 patent/EP0329736A4/en not_active Withdrawn
- 1988-07-28 AU AU22552/88A patent/AU629796B2/en not_active Ceased
- 1988-07-28 WO PCT/US1988/002573 patent/WO1989000810A1/en not_active Application Discontinuation
-
1989
- 1989-03-30 DK DK155189A patent/DK155189A/en not_active IP Right Cessation
Non-Patent Citations (3)
| Title |
|---|
| Plant Molecular Biology, Vol. 10, 1988, pages 489-497, Kluwer Academic Publishers, Dordrecht, NL; T. TURPEN et al.: "On the mechanism of cytoplasmic male sterility in the 447 line of Vicia faba", the whole document. * |
| Proc. Natl. Acad. Sci., Vol. 78, No. 11, November 1981, pages 7043-7046, US; L.K. GRILL et al.: "Identification and characterization of double-stranded RNA associated with cytoplasmic male sterility in vicia faba", Abstract, introduction, discussion. * |
| See also references of WO8900810A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2255288A (en) | 1989-03-01 |
| WO1989000810A1 (en) | 1989-02-09 |
| DK155189D0 (en) | 1989-03-30 |
| AU629796B2 (en) | 1992-10-15 |
| EP0329736A1 (en) | 1989-08-30 |
| DK155189A (en) | 1989-05-31 |
| BR8807155A (en) | 1989-10-17 |
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