EP0299150B1 - Continuous centrifuge, especially for the industrial production of proteins from human blood plasm - Google Patents

Continuous centrifuge, especially for the industrial production of proteins from human blood plasm Download PDF

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Publication number
EP0299150B1
EP0299150B1 EP88106028A EP88106028A EP0299150B1 EP 0299150 B1 EP0299150 B1 EP 0299150B1 EP 88106028 A EP88106028 A EP 88106028A EP 88106028 A EP88106028 A EP 88106028A EP 0299150 B1 EP0299150 B1 EP 0299150B1
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EP
European Patent Office
Prior art keywords
containers
plastics
continuous
container
disposed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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EP88106028A
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German (de)
French (fr)
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EP0299150A3 (en
EP0299150A2 (en
Inventor
John R. Dr. Wells
Gunthard Pautsch
Werner Kohlstette
Detlef Grabbe
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GEA Mechanical Equipment GmbH
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Westfalia Separator GmbH
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Priority to AT88106028T priority Critical patent/ATE57626T1/en
Publication of EP0299150A2 publication Critical patent/EP0299150A2/en
Publication of EP0299150A3 publication Critical patent/EP0299150A3/en
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Publication of EP0299150B1 publication Critical patent/EP0299150B1/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/0464—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with hollow or massive core in centrifuge bowl

Definitions

  • the invention relates to a continuous centrifuge, preferably for the industrial production of proteins from human blood plasma, with a cylindrical centrifugal drum provided with a plurality of concentrically arranged chambers connected to one another in the base region, in the interior of which the blood plasma or the like is fed and from the exterior of which a liquid phase is continuously withdrawn.
  • a continuous centrifuge of this type is known (DE-C 2 423 319), in which the centrifugal drum has two concentrically arranged chambers which are connected in the bottom area by channels through which blood plasma or the like. can flow from the inner chamber into the outer chamber.
  • the liquid phase flows from this outer chamber into a peeling chamber and is led outwards via a peeling disc.
  • the plasma proteins (protein) separated from the liquid phase settle in the two chambers of the centrifugal drum. After these compartments have been filled, the operator must manually scrape the protein out of these compartments. It should be noted here that the plasma proteins may not be infected by the operating personnel during this process, nor that the operating personnel may come into contact with pathogens contained in the plasma proteins.
  • centrifugal drum of the known continuous centrifuge is composed exclusively of heavy-duty metal parts, this centrifuge can be operated at the maximum speed that the centrifuge material used allows. High-speed centrifuges are required to economically obtain the plasma proteins.
  • the invention has for its object to design a continuous centrifuge, preferably for the industrial production of proteins from human blood plasma, of the type mentioned in such a way that the product is protected from contamination and the operating personnel from pathogens contained in the product while maintaining economical operation at maximum speeds are reduced and the work involved in the removal and storage of the spun off proteins is reduced.
  • the outer surfaces of the plastic containers used in the centrifugal drum are assigned support surfaces over the entire surface area, which can be formed by parts of the centrifugal drum by inserted and removable moldings or by walls of adjacent plastic containers, only forces are exerted on the surfaces of the plastic containers during centrifugal operation transferred so that plastic bags can also be used as high-speed centrifuges as plastic containers.
  • the plastic containers are delivered sterile.
  • the inlet openings and the outlet openings are closed by plugs. This stopper closure is retained even when the containers are inserted into the centrifugal drum and when the molded parts and, if necessary, the partition walls are installed.
  • the plugs are only removed shortly before the distributor, the parts delimiting the peeling chamber for the liquid phase to be discharged and the drum cover are put on.
  • the centrifuge drum After the interiors of the plastic containers are filled with proteins, the centrifuge drum is stopped and after the drum cover has been loosened and the drum parts in the inlet and outlet areas, as well as some molded parts and partitions, the filled plastic containers are removed from the centrifugal drum and stored if necessary. The plastic containers must be destroyed to remove the plasma proteins from the plastic containers.
  • the continuous centrifuge has a centrifugal drum 1 with a cylindrical lower part 2, a lid 3 and a locking ring 4 is provided, through which the lid is fixed relative to the lower part.
  • cooling jacket 5 through which cooling liquid flows, but the function of which will not be discussed further here.
  • its inner surface 6 is aligned with the inner surface 7 of the lid.
  • the filling space of the centrifugal drum is limited towards and below the axis of rotation 8 by an insert part 9, the lower base plate 10 of which consists of the plate parts 11 and 12 forming an obtuse angle.
  • the plastic containers 13 and 14 are arranged on the base plate 10 of the insert part 9 and are connected to one another via a channel 15 in the base area.
  • the structural design of the channel 15 can be seen in FIG. 4.
  • the connecting channel 15 is designed as a hose and connected to the bottom 17 of the plastic container 13 via a rib 16.
  • the channel 15, the rib 16 and the bottom 17 of the container 13 are integrally formed.
  • the channel 15 is also connected to the plastic container 14, so that the two concentrically arranged containers 13, 14 form a structural unit.
  • the container 13 is equipped with an inlet connection 18.
  • the plastic container 14 has an overhead drain channel 19 which is equipped with a drain connector 20.
  • the inlet connector 18 and the outlet connector 20 are closed by plugs 21, 22 on delivery and when the plastic container is installed in the centrifugal drum. This prevents any contamination from getting into the interior of the plastic container.
  • annular jacket 23 is arranged between the plastic container 13 and the plastic container 14 and forms support surfaces for the adjacent walls of the said containers.
  • the ring jacket 23 has recesses through which the connecting channel 15 and the drain channel 19 pass.
  • This molded part has one or more recesses for receiving a connecting channel 15 in each case. So that the entire outer surface of the connecting channel 15 formed by a hose can be supported on molded part surfaces during centrifuge operation, further molded parts 26 are inserted into the recess 25 of the molded part 24.
  • the drainage channel 19 and the drainage channels 19 are also embedded in molded parts 27, 28 which are arranged between the upper floor 29 of the inner plastic container 13 or between the upper floors 29 of the inner plastic container and the cover 3.
  • FIGS. 1 and 5 The assignment of these molded parts 27, 28 to the drain channel 19 is shown in FIGS. 1 and 5.
  • the concentrically arranged plastic containers 13 and 14 can be designed in the shape of a ring segment, so that the interior of the centrifugal drum is filled by a number of these plastic containers.
  • the use of smaller, ring segment-shaped plastic containers 13 and 14 enables the production of the plastic containers in the blowing process.
  • adjacent, radially running walls of the plastic containers are supported on one another during operation.
  • the continuous centrifuge is equipped with a distribution chamber 30, to which the blood plasma is fed via a stationary inlet pipe 31.
  • a distributor chamber 32 and a filler neck 33 are assigned to the distributor chamber 30 and engage in the inlet connector 18 of the plastic container 13.
  • a connector 34 engages in the outlet connector 20 of the outlet channel 19, through which the liquid phase drawn off passes into a peeling chamber 35.
  • the base plate 10 is provided with an annular web 36 on which the annular jacket 23 is supported.
  • the ring web 36 has recesses 37 in the area of the connecting channels 15, which are aligned with recesses 38 in the ring jacket 23.
  • the recesses are filled with a molded part 39, which forms a structural unit with the plastic containers 13, 14 and the connecting channel 15.

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  • Centrifugal Separators (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Cyclones (AREA)

Abstract

1. A continuous-flow centrifuge, preferably for the industrial production of proteins from human blood plasma, comprising a cylindrical centrifuging drum which is provided with a plurality of concentrically disposed chambers which are connected together in the bottom region, the blood plasma or the like being supplied to the inner region of the centrifuging drum and a liquid phase being continuously removed from the outer region thereof, characterised in that disposed in the centrifuging drum (1) are plastics containers (13, 14) which are arranged concentrically relative to each other and which are connected together in the bottom region by at least one passage (15) and which form chambers of a ring form or a ring segment form, the plastics container or containers (13) which is disposed towards the axis of rotation (8) of the centrifuging drum (1) having a feed connection (18) and the plastics container or containers disposed in the outer region of the centrifuging drum having a drain passage (19) disposed at the top, and that the outside surfaces of the plastics containers, the communicating passage or passages, the drain passage or passages and the feed connection or connections are supported against inside surfaces of the centrifuging drum (1) and shaped portions (24, 26, 27, 28) which are fitted into the centrifuging drum and which can be disassembled.

Description

Die Erfindung bezieht sich auf eine Durchlaufzentrifuge, vorzugsweise zur industriellen Produktion von Proteinen aus menschlichem Blutplasma, mit einer zylindrischen, mit mehreren konzentrisch angeordneten, im Bodenbereich untereinander verbundenen Kammern versehenen Schleudertrommel, in deren Innenbereich das Blutplasma oder dergleichen zugeführt und aus deren Außenbereich eine flüssige Phase kontinuierlich abgezogen wird.The invention relates to a continuous centrifuge, preferably for the industrial production of proteins from human blood plasma, with a cylindrical centrifugal drum provided with a plurality of concentrically arranged chambers connected to one another in the base region, in the interior of which the blood plasma or the like is fed and from the exterior of which a liquid phase is continuously withdrawn.

Es ist eine Durchlaufzentrifuge dieser Art bekannt (DE-C 2 423 319), bei der die Schleudertrommel zwei konzentrisch angeordnete Kammern aufweist, die im Bodenbereich durch Kanäle verbunden sind, durch die Blutplasma o.dgl. aus der Innenkammer in die Außenkammer fließen kann. Aus dieser Außenkammer fließt die flüssige Phase in eine Schälkammer und wird über eine Schälscheibe nach außen geführt. Die von der flüssigen Phase abgetrennten Plasmaproteine (Eiweiß) setzen sich in den beiden Kammern der Schleudertrommel ab. Nachdem diese Kammern gefüllt sind, muß das Eiweiß vom Bedienungspersonal manuell aus diesen Kammern her ausgeschabt werden. Hierbei ist zu beachten, daß weder die Plasmaproteine bei diesem Vorgang vom Bedienungspersonal infiziert werden dürfen, noch daß das Bedienungspersonal mit in den Plasmaproteinen enthaltenen Krankheitskeimen in Berührung kommen darf.A continuous centrifuge of this type is known (DE-C 2 423 319), in which the centrifugal drum has two concentrically arranged chambers which are connected in the bottom area by channels through which blood plasma or the like. can flow from the inner chamber into the outer chamber. The liquid phase flows from this outer chamber into a peeling chamber and is led outwards via a peeling disc. The plasma proteins (protein) separated from the liquid phase settle in the two chambers of the centrifugal drum. After these compartments have been filled, the operator must manually scrape the protein out of these compartments. It should be noted here that the plasma proteins may not be infected by the operating personnel during this process, nor that the operating personnel may come into contact with pathogens contained in the plasma proteins.

Da die Schleudertrommel der bekannten Durchlaufzentrifuge sich ausschließlich aus hochbelastbaren Metallteilen zusammensetzt, kann diese Zentrifuge mit der maximalen Drehzahl betrieben werden, die das verwendete Zentrifugenmaterial erlaubt. Zur wirtschaftlichen Gewinnung der Plasmaproteine sind hochtourige Zentrifugen erforderlich.Since the centrifugal drum of the known continuous centrifuge is composed exclusively of heavy-duty metal parts, this centrifuge can be operated at the maximum speed that the centrifuge material used allows. High-speed centrifuges are required to economically obtain the plasma proteins.

Der Erfindung liegt die Aufgabe zugrunde, eine Durchlaufzentrifuge, vorzugsweise zur industriellen Produktion von Proteinen aus menschlichem Blutplasma, der eingangs genannten Art so zu gestalten, daß unter Aufrechterhaltung eines wirtschaftlichen Betriebs mit maximalen Drehzahlen das Produkt vor Kontaminationen und das Bedienungspersonal von im Produkt enthaltenen Krankheitskeimen geschützt werden sowie der Arbeitsaufwand bei der Entnahme und Lagerung der abgeschleuderten Proteine verringert wird.The invention has for its object to design a continuous centrifuge, preferably for the industrial production of proteins from human blood plasma, of the type mentioned in such a way that the product is protected from contamination and the operating personnel from pathogens contained in the product while maintaining economical operation at maximum speeds are reduced and the work involved in the removal and storage of the spun off proteins is reduced.

Diese Aufgabe wird nach der Erfindung dadurch gelöst, daß in der Schleudertrommel konzentrisch zueinander angeordnete, im Bodenbereich durch mindestens einen Kanal miteinander verbundene ringförmige oder ringsegmentförmige Kammern bildende Kunststoffbehälter angeordnet sind, von denen der oder die der Drehachse der Schleudertrommel zugewandt liegenden Kunststoffbehälter einen Zulaufstutzen und der oder die im Außenbereich der Schleudertrommel vorgesehenen Kunststoffbehälter einen obenliegenden Ablaufkanal aufweisen,und daß die Außenflächen der Kunststoffbehälter, des oder der Verbindungskanäle, des oder der Ablaufkanäle und des oder der Zuflußstutzen sich an Innenflächen der Schleudertrommel und an die Schleudertrommel eingesetzten und demontierbaren Formteilen abstützen.This object is achieved according to the invention in that in the centrifuge drum concentrically arranged, in the bottom region interconnected by at least one channel annular or ring segment-shaped chambers forming plastic containers are arranged, of which the plastic container or the one facing the axis of rotation of the centrifugal drum has an inlet connection and the or the plastic containers provided in the outer region of the centrifugal drum have an outlet channel at the top, and that the outer surfaces of the plastic containers, the one or more connecting channels, the one or more outlet ducts and the one or more inlet connections are supported on inner surfaces of the centrifugal drum and on the removable and removable molded parts.

Da den Außenflächen der in die Schleudertrommel eingesetzten Kunststoffbehältern über den gesamten Flächenbereich Stützflächen zugeordnet sind, die durch Teile der Schleudertrommel durch eingesetzte und demontierbare Formteile oder durch Wandungen benachbarter Kunststoffbehälter gebildet werden können, sind von den Wandungen der Kunststoffbehälter während des Zentrifugalbetriebs nur Kräfte auf Stützflächen zu übertragen, so daß auch bei hochtourigen Zentrifugen als Kunststoffbehälter Kunststoffbeutel verwendet werden können.Since the outer surfaces of the plastic containers used in the centrifugal drum are assigned support surfaces over the entire surface area, which can be formed by parts of the centrifugal drum by inserted and removable moldings or by walls of adjacent plastic containers, only forces are exerted on the surfaces of the plastic containers during centrifugal operation transferred so that plastic bags can also be used as high-speed centrifuges as plastic containers.

Die Kunststoffbehälter werden steril angeliefert. Zur Aufrechterhaltung der Sterilität der Innenräume der Behälter werden die Zulauföffnungen und die Ablauföffnungen durch Stopfen verschlossen. Dieser Stopfenverschluß bleibt auch beim Einsetzen der Behälter in die Schleudertrommel und bei der Montage der Formteile und ggf. der Zwischenwände erhalten. Erst kurz vor dem Aufsetzen des Verteilers, der die Schälkammer für die abzuführende flüssige Phase begrenzenden Teile und des Trommeldeckels werden die Stopfen entfernt.The plastic containers are delivered sterile. In order to maintain the sterility of the interior of the container, the inlet openings and the outlet openings are closed by plugs. This stopper closure is retained even when the containers are inserted into the centrifugal drum and when the molded parts and, if necessary, the partition walls are installed. The plugs are only removed shortly before the distributor, the parts delimiting the peeling chamber for the liquid phase to be discharged and the drum cover are put on.

Nachdem die Innenräume der Kunststoffbehälter mit Proteinen gefüllt sind, wird die Schleudertrommel angehalten und es werden nach dem Lösen des Trommeldeckels und dem Entfernen der Trommelteile im Zulauf- und Ablaufbereich sowie einiger Formteile und Zwischenwände die gefüllten Kunststoffbehälter aus der Schleudertrommel entfernt und ggf. gelagert. Für die Entnahme der Plasmaproteine aus den Kunststoffbehältern müssen die Kunststoffbehälter zerstört werden.After the interiors of the plastic containers are filled with proteins, the centrifuge drum is stopped and after the drum cover has been loosened and the drum parts in the inlet and outlet areas, as well as some molded parts and partitions, the filled plastic containers are removed from the centrifugal drum and stored if necessary. The plastic containers must be destroyed to remove the plasma proteins from the plastic containers.

Bei der erfindungsgemäßen Zentrifuge werden Produktverlustevermieden. Eine Reinigung der Trommeleinsätze zwischen den einzelnen Chargen ist nicht mehr erforderlich. Eine Trommelauskleidung aus Sondermaterialien, z.B. aus Titan, entfällt.Product losses are avoided in the centrifuge according to the invention. It is no longer necessary to clean the drum inserts between the individual batches. A drum lining made of special materials, e.g. made of titanium, not applicable.

Weitere Kennzeichen und Merkmale der Erfindung ergeben sich aus den Unteransprüchen und der folgenden Beschreibung eines Ausführungsbeispiels, das in den Zeichnungen dargestellt ist.Further characteristics and features of the invention emerge from the subclaims and the following description of an exemplary embodiment which is illustrated in the drawings.

Es zeigen:

  • Fig. 1 die Schleudertrommel einer Durchlaufzentrifuge mit eingesetzten Kunststoffbehältern im Vertikalschnitt, und zwar im Bereich eines unteren Verbindungskanals und eines oberen Ablaufkanals,
  • Fig. 2 eine Schleudertrommel mit eingesetzten Kunststoffbehältern im Vertikalschnitt, der versetzt zu dem Verbindungskanal und zu dem Ablaufkanal gelegt wurde,
  • Fig. 3 einen Montagezwischenzustand,
  • Fig. 4 einen Schnitt nach der Linie IV-IV in Fig. 3,
  • Fig. 5 einen Schnitt nach der Linie V-V in Fig. 1 und
  • Fig. 6 einen Schnitt nach der Linie VI-VI in Fig. 3,
  • Fig. 7 einen Schnitt nach der Linie VII-VII in Fig. 3.
Show it:
  • 1 shows the centrifugal drum of a continuous centrifuge with inserted plastic containers in vertical section, specifically in the area of a lower connection channel and an upper outlet channel,
  • 2 shows a centrifugal drum with inserted plastic containers in vertical section, which was placed offset to the connecting channel and to the drain channel,
  • 3 shows an intermediate assembly state,
  • 4 shows a section along the line IV-IV in FIG. 3,
  • Fig. 5 shows a section along the line VV in Fig. 1 and
  • 6 shows a section along the line VI-VI in FIG. 3,
  • 7 is a section along the line VII-VII in Fig. 3rd

Die Durchlaufzentrifuge weist eine Schleudertrommel 1 auf, die mit einem zylindrischen Unterteil 2, einem Deckel 3 und einem Verschlußring 4 versehen ist, durch den der Deckel gegenüber dem Unterteil festgelegt wird.The continuous centrifuge has a centrifugal drum 1 with a cylindrical lower part 2, a lid 3 and a locking ring 4 is provided, through which the lid is fixed relative to the lower part.

In dem Unterteil 2 befindet sich ein Kühlmantel 5, der von Kühlflüssigkeit durchströmt wird, auf dessen Funktion hier aber nicht weiter eingegangen wird. In dem in Fig. 1 dargestellten Ausführungsbeispiel fluchtet seine Innenfläche 6 mit der Innenfläche 7 des Deckels.In the lower part 2 there is a cooling jacket 5, through which cooling liquid flows, but the function of which will not be discussed further here. In the embodiment shown in Fig. 1, its inner surface 6 is aligned with the inner surface 7 of the lid.

Der Füllraum der Schleudertrommel wird zur Drehachse 8 hin und nach unten durch ein Einsatzteil 9 begrenzt, dessen untere Bodenplatte 10 aus den einen stumpfen Winkel bildenden Plattenteilen 11 und 12 besteht.The filling space of the centrifugal drum is limited towards and below the axis of rotation 8 by an insert part 9, the lower base plate 10 of which consists of the plate parts 11 and 12 forming an obtuse angle.

In der in der Fig. 3 dargestellten Montagezwischenstellung sind auf der Bodenplatte 10 des Einsatzteiles 9 die Kunststoffbehälter 13 und 14 angeordnet, die über einen Kanal 15 im Bodenbereich untereinander verbunden sind.In the intermediate assembly position shown in FIG. 3, the plastic containers 13 and 14 are arranged on the base plate 10 of the insert part 9 and are connected to one another via a channel 15 in the base area.

Die konstruktive Ausbildung des Kanals 15 kann man aus der Fig. 4 entnehmen. Der Verbindungskanal 15 ist als Schlauch ausgebildet und über eine Rippe 16 mit dem Boden 17 des Kunststoffbehälters 13 verbunden. Der Kanal 15, die Rippe 16 und der Boden 17 des Behälters 13 sind einstückig ausgebildet. Der Kanal 15 ist ferner mit dem Kunststoffbehälter 14 verbunden, so daß die beiden konzentrisch angeordneten Behälter 13, 14 eine Baueinheit bilden.The structural design of the channel 15 can be seen in FIG. 4. The connecting channel 15 is designed as a hose and connected to the bottom 17 of the plastic container 13 via a rib 16. The channel 15, the rib 16 and the bottom 17 of the container 13 are integrally formed. The channel 15 is also connected to the plastic container 14, so that the two concentrically arranged containers 13, 14 form a structural unit.

Der Behälter 13 ist mit einem Zulaufstutzen 18 ausgerüstet. Der Kunststoffbehälter 14 weist einen obenliegenden Ablaufkanal 19 auf, der mit einem Ablaufstutzen 20 ausgerüstet ist. Der Zulaufstutzen 18 und der Ablaufstutzen 20 sind bei der Anlieferung und bei dem Einbau der Kunststoffbehälter in die Schleudertrommel durch Stopfen 21,22 verschlossen. Hierdurch wird verhindert, daß irgendwelche Verunreinigungen in den Innenraum der Kunststoffbehälter gelangen.The container 13 is equipped with an inlet connection 18. The plastic container 14 has an overhead drain channel 19 which is equipped with a drain connector 20. The inlet connector 18 and the outlet connector 20 are closed by plugs 21, 22 on delivery and when the plastic container is installed in the centrifugal drum. This prevents any contamination from getting into the interior of the plastic container.

Bei dem in den Zeichnungen dargestellen Ausführungsbeispiel ist zwischen dem Kunststoffbehälter 13 und dem Kunststoffbehälter 14 ein Ringmantel 23 angeordnet, der Stützflächen für die benachbart liegenden Wandungen der genannten Behälter bildet. Der Ringmantel 23 besitzt Ausnehmungen, durch die der Verbindungskanal 15 und der Ablaufkanal 19 hindurchführen.In the exemplary embodiment shown in the drawings, an annular jacket 23 is arranged between the plastic container 13 and the plastic container 14 and forms support surfaces for the adjacent walls of the said containers. The ring jacket 23 has recesses through which the connecting channel 15 and the drain channel 19 pass.

Unterhalb des Bodens 17 des Innenbehälters 13 bzw. der Innenbehälter ist ein Formteil 24, vorzugsweise aus Kunststoff gefertigt, angeordnet, das sich an der Bodenplatte 10 des Einsatzteils 9 abstützt und den Raum zwischen dem Boden 17 des oder der Innenbehälter und der Bodenplatte 10 ausfüllt. Dieses Formteil weist eine oder mehrere Ausnehmungen zur Aufnahme jeweils eines Verbindungskanals 15 auf. Damit die gesamte Außenfläche des durch einen Schlauch gebildeten Verbindungskanals 15 sich an Formteilflächen während des Zentrifugenbetriebes abstützen kann, werden in die Ausnehmung 25 des Formteils 24 weitere Formteile 26 eingesetzt.Below the bottom 17 of the inner container 13 or the inner container, a molded part 24, preferably made of plastic, is arranged, which is supported on the bottom plate 10 of the insert part 9 and fills the space between the bottom 17 of the inner container or the bottom plate 10. This molded part has one or more recesses for receiving a connecting channel 15 in each case. So that the entire outer surface of the connecting channel 15 formed by a hose can be supported on molded part surfaces during centrifuge operation, further molded parts 26 are inserted into the recess 25 of the molded part 24.

Aus der Fig. 4 kann man entnehmen, daß die gesamte Oberfläche des Verbindungskanals 15 in Formteilen eingebettet ist.From Fig. 4 it can be seen that the entire surface of the connecting channel 15 is embedded in molded parts.

Auch der Ablaufkanal 19 bzw. die Ablaufkanäle 19 werden in Formteilen 27,28 eingebettet, die zwisehen dem Oberboden 29 des innenliegenden Kunststoffbehälters 13 bzw. zwischen den Oberböden 29 der innenliegenden Kunststoffbehälter und dem Deckel 3 angeordnet werden.The drainage channel 19 and the drainage channels 19 are also embedded in molded parts 27, 28 which are arranged between the upper floor 29 of the inner plastic container 13 or between the upper floors 29 of the inner plastic container and the cover 3.

Die Zuordnung dieser Formteile 27,28 zu dem Ablaufkanal 19 ergeben sich aus den Fig. 1 und 5.The assignment of these molded parts 27, 28 to the drain channel 19 is shown in FIGS. 1 and 5.

Aus der Fig. 6 ergibt sich, daß die konzentrisch angeordneten Kunststoffbehälter 13 und 14 ringsegmentförmig ausgebildet sein können, so daß durch eine Reihe dieser Kunststoffbehälter der Innenraum der Schleudertrommel ausgefüllt wird. Durch die Verwendung kleinerer, ringsegmentförmiger Kunststoffbehälter 13 und 14 wird die Herstellung der Kunststoffbehälter im Blasverfahren ermöglicht.From Fig. 6 it follows that the concentrically arranged plastic containers 13 and 14 can be designed in the shape of a ring segment, so that the interior of the centrifugal drum is filled by a number of these plastic containers. The use of smaller, ring segment-shaped plastic containers 13 and 14 enables the production of the plastic containers in the blowing process.

Bei dem Ausführungsbeispiel nach der Fig. 6 stützen sich während des Betriebs benachbarte, radial verlaufende Wandungen der Kunststoffbehälter aneinander ab.In the exemplary embodiment according to FIG. 6, adjacent, radially running walls of the plastic containers are supported on one another during operation.

Die Durchlaufzentrifuge ist mit einem Verteilerraum 30 ausgerüstet, dem das Blutplasma über ein ortsfestes Einlaufrohr 31 zugeführt wird. Dem Verteilerraum 30 ist eine Sammelkammer 32 und ein Füllstutzen 33 zugeordnet, die in die Zulaufstutzen 18 der Kunststoffbehälter 13 eingreifen. In den Ablaufstutzen 20 des Ablaufkanals 19 greift ein Stutzen 34 ein, durch den die abgezogene flüssige Phase in eine Schälkammer 35 gelangt.The continuous centrifuge is equipped with a distribution chamber 30, to which the blood plasma is fed via a stationary inlet pipe 31. A distributor chamber 32 and a filler neck 33 are assigned to the distributor chamber 30 and engage in the inlet connector 18 of the plastic container 13. A connector 34 engages in the outlet connector 20 of the outlet channel 19, through which the liquid phase drawn off passes into a peeling chamber 35.

In dem dargestellten Ausführungsbeispiel (Fig. 2) ist die Bodenplatte 10 mit einem Ringsteg 36 versehen, auf dem sich der Ringmantel 23 abstützt. Der Ringsteg 36 weist im Bereich der Verbindungskanäle 15 Ausnehmungen 37 auf, die mit Ausnehmungen 38 in dem Ringmantel 23 fluchten. Die Ausnehmungen sind mit einem Formteil 39 ausgefüllt, welches eine Baueinheit mit den Kunststoffbehältern 13,14 und dem Verbindungskanal 15 bildet.In the exemplary embodiment shown (FIG. 2), the base plate 10 is provided with an annular web 36 on which the annular jacket 23 is supported. The ring web 36 has recesses 37 in the area of the connecting channels 15, which are aligned with recesses 38 in the ring jacket 23. The recesses are filled with a molded part 39, which forms a structural unit with the plastic containers 13, 14 and the connecting channel 15.

BezugszeichenReference numerals

  • 1 Schleudertrommel1 centrifugal drum
  • 2 Unterteil2 lower part
  • 3 Deckel3 lids
  • 4 Verschlußring4 locking ring
  • 5 Kühlmantel5 cooling jacket
  • 6 Innenfläche6 inner surface
  • 7 Innenfläche7 inner surface
  • 8 Drehachse8 axis of rotation
  • 9 Einsatzteil9 insert
  • 10 Bodenplatte10 base plate
  • 11 Plattenteil11 plate part
  • 12 Plattenteil12 plate part
  • 13 Kunststoffbehälter13 plastic containers
  • 14 Kunststoffbehälter14 plastic containers
  • 15 Verbindungskanal15 connecting channel
  • 16 Rippe16 rib
  • 17 Boden17 floor
  • 18 Zulaufstutzen18 inlet connection
  • 19 Ablaufkanal19 drain channel
  • 20 Ablaufstutzen20 drain socket
  • 21 Stopfen21 stoppers
  • 22 Stopfen22 plugs
  • 23 Ring23 ring
  • 24 Formteil24 molding
  • 25 Ausnehmung25 recess
  • 26 Formteil26 molding
  • 27 Formteil27 molded part
  • 28 Formteil28 molded part
  • 29 Oberboden29 topsoil
  • 30 Verteiler30 distributors
  • 31 Einlaufrohr31 inlet pipe
  • 32 Sammelkammer32 collection chamber
  • 33 Füllstutzen33 filler neck
  • 34 Stutzen34 sockets
  • 35 Schälkammer35 peeling chamber
  • 36 Ringsteg36 ring bridge
  • 37 Ausnehmung37 recess
  • 38 Ausnehmung38 recess
  • 39 Formteil39 molded part

Claims (13)

1. A continuous-flow centrifuge, preferably for the industrial production of proteins from human blood plasma, comprising a cylindrical centrifuging drum which is provided with a plurality of concentrically disposed chambers which are connected together in the bottom region, the blood plasma or the like being supplied to the inner region of the centrifuging drum and a liquid phase being continuously removed from the outer region thereof, characterised in that disposed in the centrifuging drum (1) are plastics containers (13, 14) which are arranged concentrically relative to each other and which are connected together in the bottom region by at least one passage (15) and which form chambers of a ring form or a ring segment form, the plastics container or containers (13) which is disposed towards the axis of rotation (8) of the centrifuging drum (1) having a feed connection (18) and the plastics container or containers disposed in the outer region of the centrifuging drum having a drain passage (19) disposed at the top, and that the outside surfaces of the plastics containers, the communicating passage or passages, the drain passage or passages and the feed connection or connections are supported against inside surfaces of the centrifuging drum (1) and shaped portions (24, 26, 27, 28) which are fitted into the centrifuging drum and which can be disassembled.
2. A continuous-flow centrifuge according to claim characterised in that the communicating passage (15) between an inwardly disposed and an outwardly disposed plastics container (13, 14) is fixedly connected to the containers.
3. A continuous-flow centrifuge according to claim 2 characterised in that the communicating passage (15) is in one piece with the inwardly disposed container (13), extends beneath the bottom (17) of the inwardly disposed container (13) and is connected to the bottom by a rib (16).
4. A continuous-flow centrifuge according to one of claims 1 to 3 characterised in that the centrifuging drum (1) is filled with plastics containers of ring segment form, of which a respective inwardly disposed plastics container (13) and the associated outwardly disposed plastics container (14), which containers are combined in the botton region by way of the communicating passage (15), form a respective structural unit.
5. A continuous-flow centrifuge according to claim 4 characterised in that an annular casing (23) is provided between the inner containers (13) of ring segment form and the outer containers (14) of ring segment form.
6. A continuous-flow centrifuge according to claim 3 or claim 4 characterised in that arranged beneath the bottom (17) of the inner container or containers is a shaped portion (24) which has one or more openings (25) for accommodating a respective communicating passage (15), and that the part of the opening which is adjacent to the bottom (17) of the inner container or the bottoms of the inner containers is filled by further shaped portions (26).
7. A continuous-flow centrifuge according to claim characterised in that arranged between an upper end portion (29) of the inwardly disposed plastics container (13) or the upper end portions of the inner containers and the cover (3) of the centrifuging drum are shaped portions (27, 28) which fill the space between the specified components and in which the drain passages (19) of the outer container or containers are embedded.
8. A continuous-flow centrifuge according to claim characterised in that the adjacent walls of two plastics containers (13, 14) lie directly one against the other.
9. A continuous-flow centrifuge according to claim characterised in that the boundary wall, which is towards the axis of rotation (8) of the centrifuging drum (1), of the plastic container or containers (13) is supported against an insert portion (9) of the centrifuging drum, the insert portion (9) delimiting the filling space of the centrifuging drum (1) towards the axis of rotation (8) and at the bottom thereof.
10. A continuous-flow centrifuge according to claim 9 characterised in that the insert portion (9) has a bottom plate (10) which comprises the plate portions (11 and 12) and on which are supported the bottom of the plastics container (14) and one or more shaped portions (24) which are arranged beneath the bottom (17) of the plastics container (13) or the plastics containers (13).
11. A continuous-flow centrifuge according to claim 10 characterised in that the bottom plate (10) is provided with an annular limb portion (36) on which the annular casing (23) is supported and which in the region of the communicating passages (15) has openings (37) aligned with openings (30) in the annular casing (23), wherein the openings (37, 38) are filled with a shaped portion (39) which forms a structural unit with the plastics containers (13, 14) and the communicating passage (15).
12. A continuous-flow centrifuge according to one of the preceding claims characterised in that the shaped portions (24, 26, 27, 28, 39) are made from plastics material.
13. A continuous-flow centrifuge according to claim characterised by a distributor space (30) with which are associated a collecting chamber (32) and filling connection (33), which engage into the feed connections (18) of the inner containers.
EP88106028A 1987-07-13 1988-04-15 Continuous centrifuge, especially for the industrial production of proteins from human blood plasm Expired - Lifetime EP0299150B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT88106028T ATE57626T1 (en) 1987-07-13 1988-04-15 CONTINUOUS CENTRIFUGE, PREFERABLE FOR THE INDUSTRIAL PRODUCTION OF PROTEINS FROM HUMAN BLOOD PLASMA.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3723092 1987-07-13
DE3723092A DE3723092C1 (en) 1987-07-13 1987-07-13 Continuous centrifuge for the industrial production of proteins from human blood plasma

Publications (3)

Publication Number Publication Date
EP0299150A2 EP0299150A2 (en) 1989-01-18
EP0299150A3 EP0299150A3 (en) 1989-05-24
EP0299150B1 true EP0299150B1 (en) 1990-10-24

Family

ID=6331464

Family Applications (1)

Application Number Title Priority Date Filing Date
EP88106028A Expired - Lifetime EP0299150B1 (en) 1987-07-13 1988-04-15 Continuous centrifuge, especially for the industrial production of proteins from human blood plasm

Country Status (8)

Country Link
US (1) US4950220A (en)
EP (1) EP0299150B1 (en)
JP (1) JPH0659419B2 (en)
AT (1) ATE57626T1 (en)
AU (1) AU602829B2 (en)
DE (2) DE3723092C1 (en)
ES (1) ES2018318B3 (en)
GR (1) GR3001263T3 (en)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4900298A (en) * 1987-08-21 1990-02-13 Cobe Laboratories, Inc. Centrifuge drive and support assembly
US20030205538A1 (en) 2002-05-03 2003-11-06 Randel Dorian Methods and apparatus for isolating platelets from blood
US7832566B2 (en) 2002-05-24 2010-11-16 Biomet Biologics, Llc Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles
US20060278588A1 (en) 2002-05-24 2006-12-14 Woodell-May Jennifer E Apparatus and method for separating and concentrating fluids containing multiple components
US7845499B2 (en) 2002-05-24 2010-12-07 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
DE102004046396A1 (en) * 2004-09-24 2006-04-13 Land Baden-Württemberg, vertreten durch das Ministerium für Wissenschaft, Forschung und Kunst Baden-Württemberg, vertreten durch den Minister A particle sedimentation apparatus and method for performing a particle sedimentation
WO2006086201A2 (en) 2005-02-07 2006-08-17 Hanuman Llc Platelet rich plasma concentrate apparatus and method
US7866485B2 (en) 2005-02-07 2011-01-11 Hanuman, Llc Apparatus and method for preparing platelet rich plasma and concentrates thereof
ES2426941T3 (en) 2005-02-07 2013-10-25 Hanuman Llc Apparatus and procedure of platelet rich plasma concentrates
US8567609B2 (en) 2006-05-25 2013-10-29 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
JP5479319B2 (en) 2007-04-12 2014-04-23 バイオメット・バイオロジックス・リミテッド・ライアビリティ・カンパニー Buoy suspension fractionation system
US8328024B2 (en) 2007-04-12 2012-12-11 Hanuman, Llc Buoy suspension fractionation system
WO2009108890A1 (en) 2008-02-27 2009-09-03 Biomet Biologics, Llc Methods and compositions for delivering interleukin-1 receptor antagonist
WO2009111338A1 (en) 2008-02-29 2009-09-11 Biomet Manufacturing Corp. A system and process for separating a material
US8012077B2 (en) 2008-05-23 2011-09-06 Biomet Biologics, Llc Blood separating device
US20100158893A1 (en) * 2008-12-19 2010-06-24 Baxter International Inc. Systems and methods for obtaining immunoglobulin from blood
US8187475B2 (en) 2009-03-06 2012-05-29 Biomet Biologics, Llc Method and apparatus for producing autologous thrombin
US8313954B2 (en) 2009-04-03 2012-11-20 Biomet Biologics, Llc All-in-one means of separating blood components
US9011800B2 (en) 2009-07-16 2015-04-21 Biomet Biologics, Llc Method and apparatus for separating biological materials
US8591391B2 (en) 2010-04-12 2013-11-26 Biomet Biologics, Llc Method and apparatus for separating a material
US9642956B2 (en) 2012-08-27 2017-05-09 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US10143725B2 (en) 2013-03-15 2018-12-04 Biomet Biologics, Llc Treatment of pain using protein solutions
US9950035B2 (en) 2013-03-15 2018-04-24 Biomet Biologics, Llc Methods and non-immunogenic compositions for treating inflammatory disorders
US10208095B2 (en) 2013-03-15 2019-02-19 Biomet Manufacturing, Llc Methods for making cytokine compositions from tissues using non-centrifugal methods
US9895418B2 (en) 2013-03-15 2018-02-20 Biomet Biologics, Llc Treatment of peripheral vascular disease using protein solutions
US20140271589A1 (en) 2013-03-15 2014-09-18 Biomet Biologics, Llc Treatment of collagen defects using protein solutions
DE102015108272A1 (en) 2014-05-28 2015-12-03 Gea Mechanical Equipment Gmbh separator
US9713810B2 (en) 2015-03-30 2017-07-25 Biomet Biologics, Llc Cell washing plunger using centrifugal force
US9757721B2 (en) 2015-05-11 2017-09-12 Biomet Biologics, Llc Cell washing plunger using centrifugal force

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1101549A (en) * 1911-10-24 1914-06-30 Balthasar Hoffman Centrifugal separating-machine.
US1370326A (en) * 1919-08-19 1921-03-01 Laval Separator Co De Centrifugal clarifier
US1371259A (en) * 1920-04-19 1921-03-15 Norris Nelson Howard Centrifugal bowl for use on centrifugal separators
US3708111A (en) * 1969-12-19 1973-01-02 P Sheeler Apparatus and method for gradient zonal centrifugation
US3858793A (en) * 1973-02-28 1975-01-07 Donaldson Co Inc Cartridge centrifuge
DE2423319C3 (en) * 1974-05-14 1976-10-14 Westfalia Separator Ag CENTRIFUGE WITH A CYLINDRICAL FULL-WALLED TURN-DRUM, THE CASE OF WHICH IS CONTINUOUSLY COOLABLE BY A CIRCULATED FIRST COOLING MEDIUM
US4303193A (en) * 1979-01-22 1981-12-01 Haemonetics Corporation Apparatus for separating blood into components thereof
EP0026417B1 (en) * 1979-09-22 1983-12-14 Firma Andreas Hettich Centrifuge with system of bloodbag for the separation of blood components
DE2938367A1 (en) * 1979-09-22 1981-06-19 Fa. Andreas Hettich, 7200 Tuttlingen Sepn. of blood constituents using a centrifuge - where blood bag is squeezed during centrifuging to obtain efficient sepn. of erythrocytes from other constituents
US4344560A (en) * 1979-11-02 1982-08-17 Asahi Kasei Kogyo Kabushiki Kaisha Container, apparatus and method for separating platelets
US4304357A (en) * 1980-06-16 1981-12-08 Haemonetics Corporation Blood processing centrifuge

Also Published As

Publication number Publication date
EP0299150A3 (en) 1989-05-24
EP0299150A2 (en) 1989-01-18
ES2018318B3 (en) 1991-04-01
JPS6434456A (en) 1989-02-03
AU602829B2 (en) 1990-10-25
AU1634888A (en) 1989-01-19
DE3723092C1 (en) 1989-01-26
US4950220A (en) 1990-08-21
JPH0659419B2 (en) 1994-08-10
ATE57626T1 (en) 1990-11-15
GR3001263T3 (en) 1992-08-25
DE3860877D1 (en) 1990-11-29

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