EP0296544A3 - Immunometrical test process - Google Patents

Immunometrical test process Download PDF

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Publication number
EP0296544A3
EP0296544A3 EP88109886A EP88109886A EP0296544A3 EP 0296544 A3 EP0296544 A3 EP 0296544A3 EP 88109886 A EP88109886 A EP 88109886A EP 88109886 A EP88109886 A EP 88109886A EP 0296544 A3 EP0296544 A3 EP 0296544A3
Authority
EP
European Patent Office
Prior art keywords
substance
immunometrical
test process
excess
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP88109886A
Other languages
German (de)
French (fr)
Other versions
EP0296544A2 (en
Inventor
Gerd Dr. Schnorr
Helmut Dr. Strecker
Peter Dr. Molz
Guido Dr. Simons
Heinz Jürgen Dr. Skrzipczyk
Hans-Peter Dr. Harthus
Götz Dr. Walter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of EP0296544A2 publication Critical patent/EP0296544A2/en
Publication of EP0296544A3 publication Critical patent/EP0296544A3/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/686—Anti-idiotype

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Gegenstand der Erfindung ist ein immunometrisches Bestimmungs­ verfahren, bei dem zur Vermeidung unspezifisch erhöhter oder erniedrigter Werte für das zu bestimmende Antigen dem unmarkier­ ten (1) und/oder dem markierten Antikörper (2) im Überschuß eine Substanz (Suppressions-Substanz) (6) zugesetzt wird, welche aufgrund ihrer großen Ähnlichkeit mit den verwendeten Reagenz-­ Antikörpern und aufgrund ihres hohen Überschusses, die mit den Antikörpern interferierende Substanz (3) selektiv abfängt und somit eine Beeinflussung des Assays ausschließt. The invention relates to an immunometric determination procedure in which to avoid unspecifically increased or lower values for the antigen to be determined the unlabeled ten (1) and / or the labeled antibody (2) in excess a substance (suppression substance) (6) is added which due to their great similarity to the reagent used Antibodies and because of their high excess that with the antibody interfering substance (3) selectively intercepts and thus rules out any influence on the assay.

EP88109886A 1987-06-25 1988-06-22 Immunometrical test process Withdrawn EP0296544A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3720983 1987-06-25
DE19873720983 DE3720983A1 (en) 1987-06-25 1987-06-25 IMMUNOMETRIC DETERMINATION PROCEDURE

Publications (2)

Publication Number Publication Date
EP0296544A2 EP0296544A2 (en) 1988-12-28
EP0296544A3 true EP0296544A3 (en) 1989-09-06

Family

ID=6330267

Family Applications (1)

Application Number Title Priority Date Filing Date
EP88109886A Withdrawn EP0296544A3 (en) 1987-06-25 1988-06-22 Immunometrical test process

Country Status (9)

Country Link
EP (1) EP0296544A3 (en)
JP (1) JPS6428560A (en)
AU (1) AU620811B2 (en)
CA (1) CA1304683C (en)
DE (1) DE3720983A1 (en)
DK (1) DK349288A (en)
FI (1) FI883057A (en)
NO (1) NO882806L (en)
PT (1) PT87810B (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989010974A1 (en) * 1988-05-11 1989-11-16 Trustees Of The Sisters Of Charity Of Australia Enzyme immunoassay system
WO1990010226A1 (en) * 1989-02-21 1990-09-07 Pitman-Moore, Inc. Preventing false-positive results in elisa-assay methods
DE3915135A1 (en) * 1989-05-09 1990-11-15 Boehringer Mannheim Gmbh PROCESS FOR DETECTING SPECIFICALLY BINDERABLE SUBSTANCES IN KOERPERFLUESSIGKEITEN
DE69228817T2 (en) * 1991-07-26 1999-09-23 Dade Chemistry Systems Inc SIGNAL DETECTION CHECK IN THE PRESENCE OF A SUSPENDED SOLID CARRIER
DE4227102C2 (en) * 1992-08-17 2002-11-14 Dade Behring Marburg Gmbh Immunochemical method for the detection of an analyte
DE4339795C2 (en) * 1993-11-17 1995-10-05 Hunger Hans Dieter Dr Method for the detection of biomolecules
DE4434093A1 (en) * 1994-09-23 1996-03-28 Boehringer Mannheim Gmbh Method for the qualitative and / or quantitative detection of a substance to be determined
DE19828466A1 (en) * 1998-06-26 1999-12-30 Roche Diagnostics Gmbh Suppressor peptides derived from antibodies for use in immunoassays
AU766551C (en) * 1998-08-28 2005-02-17 Genentech Inc. Human anti-factor IX/IXa antibodies
JP4839530B2 (en) * 2001-06-08 2011-12-21 東ソー株式会社 Method and reagent composition for reducing measurement interference
JP5306901B2 (en) * 2009-05-25 2013-10-02 ベックマン コールター， インコーポレイテッド Blood type determination method and erythrocyte solid phase container therefor
CN101963618B (en) * 2010-08-31 2014-03-12 上海铭源数康生物芯片有限公司 Method for identifying heterophilic antibody interference in antibody microarray system and antibody microarray chip using same for detecting target antigen
TW202342979A (en) 2021-12-28 2023-11-01 日商積水醫療股份有限公司 Detection method and detection reagent

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2365800A1 (en) * 1976-09-25 1978-04-21 Behringwerke Ag METHOD FOR DETERMINING A REAGENT FOR AN IMMUNOLOGICAL REACTION

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935339A (en) * 1985-05-07 1990-06-19 Nichols Institute Diagnostics Delayed solid phase immunologic assay
AU6839587A (en) * 1985-12-10 1987-06-30 Murex Corp. Particle-bound binding component immunoassay

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2365800A1 (en) * 1976-09-25 1978-04-21 Behringwerke Ag METHOD FOR DETERMINING A REAGENT FOR AN IMMUNOLOGICAL REACTION

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CLINICAL CHEMISTRY, Band 32, Nr. 8, August 1986, Seiten 1491-1495, Winston, US; L.M. BOSCATO et al.: "Incidence and specificity of interference in two-site immunoassays" *

Also Published As

Publication number Publication date
DK349288D0 (en) 1988-06-24
JPS6428560A (en) 1989-01-31
EP0296544A2 (en) 1988-12-28
NO882806D0 (en) 1988-06-24
PT87810B (en) 1992-10-30
AU1836688A (en) 1989-01-05
PT87810A (en) 1989-05-31
DE3720983A1 (en) 1989-01-05
NO882806L (en) 1988-12-27
FI883057A (en) 1988-12-26
CA1304683C (en) 1992-07-07
FI883057A0 (en) 1988-06-23
DK349288A (en) 1988-12-26
AU620811B2 (en) 1992-02-27

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