EP0264866A3 - Method for the determination of the presence of diagnostically relevant substances, particularly antibodies or antigens, by the "elisa" method using photometric evaluation - Google Patents

Method for the determination of the presence of diagnostically relevant substances, particularly antibodies or antigens, by the "elisa" method using photometric evaluation Download PDF

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Publication number
EP0264866A3
EP0264866A3 EP19870115218 EP87115218A EP0264866A3 EP 0264866 A3 EP0264866 A3 EP 0264866A3 EP 19870115218 EP19870115218 EP 19870115218 EP 87115218 A EP87115218 A EP 87115218A EP 0264866 A3 EP0264866 A3 EP 0264866A3
Authority
EP
European Patent Office
Prior art keywords
diagnostically relevant
titer
relevant substances
antigens
elisa
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19870115218
Other languages
German (de)
French (fr)
Other versions
EP0264866A2 (en
Inventor
Hans-Detlef Dr. Dopatka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE3639279A external-priority patent/DE3639279C3/en
Application filed by Behringwerke AG filed Critical Behringwerke AG
Publication of EP0264866A2 publication Critical patent/EP0264866A2/en
Publication of EP0264866A3 publication Critical patent/EP0264866A3/en
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • G01N33/5761—Hepatitis B
    • G01N33/5764—Hepatitis B surface antigen

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Communicable Diseases (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Angegeben wird ein Verfahren zur Detektion und stufenlo­ sen Quantifizierung von diagnostisch relevanten Substan­ zen, wobei ein einziger Probenansatz für die Untersuchung der Probe ausreicht. Als Test können ELISA, RIA, Nuklein­ säure-Hybridisierung, Nephelometrie u. a. Bestimmungsme­ thoden verwendet werden. Als diagnostsich relevante Substanzen werden z. B. Antigene, Antikörper der ver­ schiedenen Immunglobulinklassen, Nukleinsäuren oder Stoffwechselprodukte von medizinischer Bedeutung nach­ gewiesen. An einer Probe in der gewählten Testverdünnung mit eingefärbten enzymatisch markierten Antigen-/Anti­ körperkomplexen wird die Extinktion gemessen und aus dem erhaltenen Signal AOD der Titer rechnerisch nach der Formel:

    lg Titer= alpha . A

ermittelt, der dem durch Verdünnungsreihen erhaltbaren Endverdünnungstiter mit guter Genauigkeit gleichgesetzt werden kann. Die Werte für alpha und beta werden in getrennten Versuchsreihen experimentell ermittelt. A method for the detection and stepless quantification of diagnostically relevant substances is specified, whereby a single sample preparation is sufficient for the examination of the sample. ELISA, RIA, nucleic acid hybridization, nephelometry and other determination methods can be used as the test. As diagnostically relevant substances z. B. antigens, antibodies of different immunoglobulin classes, nucleic acids or metabolic products of medical importance. The extinction is measured on a sample in the selected test dilution with colored enzymatically labeled antigen / antibody complexes and the titer is calculated from the signal A OD obtained using the formula:

lg titer = alpha. A

determined, which can be equated with good accuracy to the final dilution titer obtainable by dilution series. The values for alpha and beta are determined experimentally in separate test series.

EP19870115218 1986-10-24 1987-10-17 Method for the determination of the presence of diagnostically relevant substances, particularly antibodies or antigens, by the "elisa" method using photometric evaluation Withdrawn EP0264866A3 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3636271 1986-10-24
DE3636271 1986-10-24
DE3639279A DE3639279C3 (en) 1986-10-24 1986-11-17 Method for the quantitative determination of antibodies or antigens according to the ELISA method by photometric evaluation
DE3639279 1986-11-17

Publications (2)

Publication Number Publication Date
EP0264866A2 EP0264866A2 (en) 1988-04-27
EP0264866A3 true EP0264866A3 (en) 1990-12-27

Family

ID=25848767

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19870115218 Withdrawn EP0264866A3 (en) 1986-10-24 1987-10-17 Method for the determination of the presence of diagnostically relevant substances, particularly antibodies or antigens, by the "elisa" method using photometric evaluation

Country Status (5)

Country Link
EP (1) EP0264866A3 (en)
JP (1) JP2595267B2 (en)
AU (1) AU602170B2 (en)
MX (1) MX168603B (en)
NO (1) NO170908C (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3718140A1 (en) * 1987-05-29 1988-12-08 Boehringer Mannheim Gmbh HETEROGENIC IMMUNOASSAY
DE3842580A1 (en) * 1988-12-17 1990-06-21 Behringwerke Ag METHOD FOR IMPROVING THE ACCURACY AND REPRODUCIBILITY OF THE MEASURED DATA OF IMMUNOMETRIC TESTS
DE3911361A1 (en) * 1989-04-07 1990-10-11 Behringwerke Ag METHOD FOR DETERMINING ANTIBODIES AGAINST EXHIBITORS OF INFECTIOUS DISEASES IN BODY LIQUIDS, MEANS THEREFOR AND THEIR USE IN THIS METHOD

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3122305A1 (en) * 1981-06-05 1983-01-05 Vladimir Florin 3004 Isernhagen Niculescu Immunological diagnostic method for detecting and determining antibodies

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58113758A (en) * 1981-12-26 1983-07-06 Shimadzu Corp Analysis for immune globulin
JPS58211659A (en) * 1982-06-03 1983-12-09 Nitsusui Seiyaku Kk Handy and quick assay of serum crp by immunological nephelometry
JPS6276464A (en) * 1985-09-30 1987-04-08 Shimadzu Corp Multinominal automatic analyzer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3122305A1 (en) * 1981-06-05 1983-01-05 Vladimir Florin 3004 Isernhagen Niculescu Immunological diagnostic method for detecting and determining antibodies

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
IMMUNITÄT UND INFEKTION, Band 9, 1981, Seiten 33-39, München, DE; H. ZAHNER et al.: "Ein Beitrag zur stufenlosen Antikörperbestimmung mit dem "Enzyme Linked Immunosorbent Assay" (ELISA)" *
JOURNAL OF CLINICAL PATHOLOGY, Band 33, 1980, Seiten 635-639, London, GB; A.M. VAN LOON et al.: "Enzyme-linked immunosorbent assay for quantitation of toxoplasma antibodies in human sera" *
JOURNAL OF CLINICAL PATHOLOGY, Band 34, 1981, Seiten 665-669, London, GB; A.M. VAN LOON et al.: "Enzyme-linked immunosorbent assay for measurement of antibody against cytomegalovirus and rubella virus in a single serum dilution" *

Also Published As

Publication number Publication date
JPS63115060A (en) 1988-05-19
AU8008487A (en) 1988-04-28
NO874405L (en) 1988-04-25
JP2595267B2 (en) 1997-04-02
EP0264866A2 (en) 1988-04-27
NO874405D0 (en) 1987-10-22
AU602170B2 (en) 1990-10-04
NO170908B (en) 1992-09-14
NO170908C (en) 1992-12-23
MX168603B (en) 1993-06-01

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Inventor name: DOPATKA, HANS-DETLEF, DR.