EP0250243B1 - Burette - Google Patents

Burette Download PDF

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Publication number
EP0250243B1
EP0250243B1 EP87305422A EP87305422A EP0250243B1 EP 0250243 B1 EP0250243 B1 EP 0250243B1 EP 87305422 A EP87305422 A EP 87305422A EP 87305422 A EP87305422 A EP 87305422A EP 0250243 B1 EP0250243 B1 EP 0250243B1
Authority
EP
European Patent Office
Prior art keywords
burette
reagent
diaphragm
reagent container
linear actuator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP87305422A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP0250243A2 (en
EP0250243A3 (en
Inventor
Timothy John Sly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
WR Grace and Co Conn
Original Assignee
WR Grace and Co Conn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by WR Grace and Co Conn filed Critical WR Grace and Co Conn
Priority to AT87305422T priority Critical patent/ATE72773T1/de
Publication of EP0250243A2 publication Critical patent/EP0250243A2/en
Publication of EP0250243A3 publication Critical patent/EP0250243A3/en
Application granted granted Critical
Publication of EP0250243B1 publication Critical patent/EP0250243B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
    • B01L3/0206—Burettes, i.e. for withdrawing and redistributing liquids through different conduits of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/027—Digital display, e.g. LCD, LED

Definitions

  • the present invention relates to a burette which can be used for on-site titrimetric analysis, for example monitoring water quality.
  • An electrically operated pipette is also known, from EP-A-0 152 120 (Rainin Instrument Company) in which a stepper motor drives the pipette plunger in both an extension direction for dispensing liquid from the pipette and a retraction direction for taking up liquid into the pipette, again with a digital counter to indicate the displacement of the plunger and hence the quantity of liquid taken in or dispensed.
  • EP-A-0 152 120 discloses the possibility of several different sizes of dispensing chambers for use with the same drive module in order to allow different quantity ranges of liquid to be handled in the pipette.
  • the present invention provides a burette comprising a housing, means on the housing for receiving a releasable reagent container including a displaceable plunger and a discharge passage, linear actuator means for driving the plunger in a reagent discharge direction, and display means responsive to displacement of the linear actuator for indicating the dispensing travel of the plunger, wherein the removable reagent container includes a self-re-sealing disphragm and means for piercing the diaphragm for dispensing of reagent from the container and for allowing the diaphragm to re-seal at termination of dispensing.
  • the releasable reagent containers may be sealed disposable plastic cartridges which self-re-seal after use, thereby simplifying greatly the handling of the reagent and eliminating the dangers of chemical spillage.
  • the releasable reagent container may be made self-re-sealing by means of a diaphragm which can re-seal satisfactorily at least 20 times, so a considerable number of separate titrimetric analysis operations can be carried out on one reagent container before it is eventually empty and needs to be disposed of.
  • the display means preferably give a direct read-out of analyte concentration (by appropriate relationship of the reagent concentration to the rate of dispensing of the reagent). This considerably simplifies the operator's task of titrimetric analysis.
  • burette in accordance with the present invention can be equipped with interface means allowing the motorised burette to be controlled from a programmed or programmable controller which will effect standard titrimetric analysis according to a pre-determined programme.
  • the optional ability to recharge the battery by way of the interface adds to the convenience of operation in the field.
  • the burette illustrated in Figure 1 comprises a main housing 1 which has at its front end a recess 2 for a pre-packed container 3 including a reagent to be used during titrimetric analysis for on-site water monitoring.
  • a plunger 4 within the reagent container 3 is held in place by a flange 5 so that even when the container 3 is outside the burette body 1 the plunger will be held in situ.
  • the reagent container 3 is held in place by means of a clip 6 on the front end of the body 1, positioned so as not to obstruct a cannula 7 through which reagent is to be dispensed during analysis.
  • the dispensing of reagent from the container 3 is achieved by means of a stepping motor 8 co-operating with a linearly displaceable shaft 9 so that the stepping motor and the shaft together form a linear actuator.
  • the shaft 9 is of a cross-sectional shape, for example splined, which prevents it from rotating and hence it is subject solely to linear reciprocation generated by way of a screw (not shown) within the rotor of the stepping motor 8.
  • the housing 1 further includes a rechargeable battery 10 to provide power to drive the stepping motor 8.
  • a rechargeable battery 10 to provide power to drive the stepping motor 8.
  • On the exterior of the recess for the battery 10 are two control buttons 11 and 12 whose function will be described later.
  • a digital indicator 13 for example an LCD indicator
  • an interface socket 14 allowing the burette to be connected by means of a suitable multi-pin plug to a battery-charging power supply and/or to a programmable or programmed controller (not shown), for example a microcomputer.
  • This controller is then able to instruct the burette to carry out automatically pre-programmed analysis sequences, if desired.
  • the burette shown in Figure 1 will normally be operated with the cannula 7 at the lower end and the display 13 uppermost where it can be viewed by the operator.
  • the reagent container 3 be of a self-sealing type so that the reagent in the container will be kept safe against inadvertent dilution or loss until such time as dispensing is required. Normally there will be some air trapped within the reagent container in order to allow for expansion of the liquid reagent. In such cases where the reagent is oxygen-sensitive, the container 3 may be topped up with an inert gas such as nitrogen which will of course remain trapped within the container during storage and transport of the containers and during use of the burette.
  • an inert gas such as nitrogen which will of course remain trapped within the container during storage and transport of the containers and during use of the burette.
  • a switch 16 in the reagent container recess 2 is tripped only when a reagent container 3 is in place.
  • FIG. 2 The block diagram shown in Figure 2 employs the same reference numerals as Figure 1 for those components which are shown in both drawings, but additionally illustrates the burette control circuit 17 and a burette driver circuit 18 which generate the appropriate pulses to drive the stepping motor 8.
  • the battery 10 has its output branched to drive the display 13, the control circuit 17, and the burette driver circuit 18. Equally, as mentioned above, it has an input from the interface 14 to allow for recharging of the battery when desired.
  • the interface 14 has two further outputs which join up with the output lines from the control buttons 11 and 12 so as to provide inputs to the control circuit 17.
  • the button 11, and the appropriate by-passing output from the interface 14, causes the control circuit 17 to generate a stream of control pulses to the driver circuit 18 which in turn generates power pulses to the motor 8.
  • the push button 12 and the appropriate output from the interface 14 provides a means of resetting the digital display 13 to zero.
  • the push button 12 has an additional function in association with the control circuit 17 in that the control circuit 17 is such that when the two push buttons 11 and 12 are depressed simultaneously (or the corresponding outputs of the interface 14 are energised simultaneously) the control pulses emitted by the control circuit 17 and the corresponding drive pulses emitted by the driver circuit 18 cause the stepper motor 8 to cycle rapidly for advancing the drive shaft 9 into engagement with the plunger 4 of, for example, a newly inserted reagent container 3 to prime the burette. As soon as the operator sees a quantity of liquid expelled from the cannula 7 he releases the two push buttons 11 and 12.
  • buttons 12 alone in order to reset the display 13 to zero and then when dispensing of reagent from the container 3 during titrimetric analysis is required the operator presses button 11 alone, causing the stepper motor 8 to cycle slowly. When the operator releases the button 11 the stepper motor stops.
  • Each pulse generated by the control circuit 17 is recorded on the digital display 13 and is delivered to any remote programme controller connected to the burette via interface 14. Each such pulse corresponds to a known fixed volume of liquid dispensed from the cartridge.
  • the concentration of the chemical reagent used in the container 3 is related to the incremental quantity of liquid dispensed per control pulse from the control circuit 17 such that the value visible on the digital display 13 at the end of the titration procedure corresponds to the concentration of the analyte being determined by titration, for example expressed in milligrams per litre.
  • the burette includes pulse-emitting means to deliver, via the interface socket, pulses proportional to the volume of liquid dispensed.
  • the control circuit includes means for counting the number of control pulses emitted from the fully retracted position of the shaft 9, for example from the instant of installation of a fresh reagent container 3 tripping the switch 16, and the counter triggers the control circuit to cease to deliver pulses when the number of pulses counted is equivalent to travel of the shaft 9 to its limit position in which the plunger 4 has run out of travel.
  • some mechanical sensing means may be incorporated for signalling to the control circuit 17 when the plunger 4 is fully forward. In this way it is possible to ensure that no damage to the drive transmission occurs at full travel position.
  • a further optional characteristic is that when the reagent container 3 is removed, and the switch 16 tripped to its alternative position, the control circuit automatically instructs the driver circuit 18 to drive the stepper motor 8 in the reverse direction until the drive shaft 9 is fully retracted. When the shaft 9 reaches this rearward limit of travel, the contents of the counter register are reset to zero and power is disconnected from the driver circuit 17 leaving the burette in its quiescent state ready to receive a reagent container.
  • Figure 3 shows in more detail the reagent container 3 and its end fitting carrying the cannula 7. If desired, a short length of small bore plastic tubing may be slipped over the end of the cannula 7 to facilitate dispensing reagent without splashing.
  • the reagent container 3 has its plunger 4 provided with a recess 19 intended to accommodate the air bubble when the container 3 is in the operative configuration, and hence the inadvertent dispensing of air along with the reagent liquid is avoided.
  • the end of the reagent container 3 opposite the plunger-retaining flange 5 is closed by a screw cap 20 which supports an end extension 21 on which an end fitting 22 is secured by way of a bayonet fastening means 23.
  • the end fitting 22 includes a part of the cannula 7 having a sharpened point 24 which pierces a self-sealing diaphragm 25 all of which are shown in Figure 4.
  • the cannula tip 24 When, on the other hand, the end fitting 22 is released from the screw cap 20 the cannula tip 24 is withdrawn from the diaphragm 25, leaving the diaphragm to re-seal, and also allowing a neoprene sheath 26 on the cannula 7 to extend into the Figure 4 position to maintain the cannula tip 24 safe against pollution. It will of course be understood that when the cannula tip 24 pierces the diaphragm 25 the neoprene sheath 26 yields concertina-fashion to expose the cannula tip 24 to the diaphragm.
  • the diaphragm 25 is a plug of natural "india” rubber having a thickness of between 2.0 and 2.5 mm and subjected to radial compression on insertion, in order to enhance its self-sealing properties.
  • the provision of the interface 14 allows the illustrated burette either to be used as a hand-held on-site digital burette for titration, or to be used as part of a fully automated titration apparatus when linked to an appropriate controller such as a microcomputer.
  • a 25 cm3 aliquot of the water to be analysed is placed in a small flask or beaker, and the water is then dosed with one or two drops of BDH 4.5 indicator, a proprietary chemical indicator solution which undergoes a colour change at pH 4.5 to cause the solution to become blue in colour.
  • BDH 4.5 indicator a proprietary chemical indicator solution which undergoes a colour change at pH 4.5 to cause the solution to become blue in colour.
  • a pre-filled reagent container 3 containing 0.0618 mol.dm ⁇ 3 sulphuric acid is then inserted into the burette housing 1 and is clipped in place by operation of the clip 6 of Figure 1.
  • the priming procedure described above under which the drive shaft 9 is advanced rapidly by simultaneous depression of the two push buttons 11 and 12, is carried out to bring the drive shaft into engagement with the plunger 4 of the reagent container, and to ensure that the liquid/air interface in the container 3 reaches the discharge end of the cannula 7.
  • the value shown on the digital display 13 of the burette is noted, and by virtue of the specially chosen gearing of the stepper motor 8 and the dimensions of the reagent container 3, related to the above-mentioned concentration of the sulphuric acid in the container 3, the value displayed corresponds to the total alkalinity of the water sample expressed in mg.dm ⁇ 3 CaCO3.
  • burette immediately for similar titrimetric analysis on a further sample of water, or to remove the end fitting 22 ( Figures 3 and 4) with the cannula to cause the self-sealing diaphragm 25 to re-seal and the neoprene sheath 26 to recover the tip 24 of the cannula 7.
  • the burette can then be transported to a fresh site for further titrimetric analysis with the same reagent container 3 or be stored for some future use.
  • the end fitting 22 is first of all re-attached to re-open the diaphragm 25, and then the button 11 is depressed to cause the stepper motor 8 to drive the plunger 4 forwardly until the end fitting 22 ( Figures 3 and 4) and the cannula 7 are once again filled with reagent.
  • the container can be removed by release of the clip 6, whereupon the switch 16 will be released to trigger the control circuit 17 to reverse the stepper motor 8 in order to retract fully the shaft 9 and to return the burette to its quiescent state.
  • titrimetric determinations can be carried out in an analogous manner, using a suitable combination of reagent and indicator for each particular analyte to be determined, and preferably using a reagent concentration related to the incremental volume of liquid dispensed so as to give a direct read out, as in the above example.
  • Other applications for the invention may include the performance of fully automatic titrimetric analysis, the intermittent or continuous dispensing of bio-chemical reagents in luminescence assays of biological material, and the intermittent or continuous dispensing of calibration standards or samples or reagents into chemical sensing systems for industrial process monitoring.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Steroid Compounds (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Opening Bottles Or Cans (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP87305422A 1986-06-19 1987-06-18 Burette Expired - Lifetime EP0250243B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT87305422T ATE72773T1 (de) 1986-06-19 1987-06-18 Buerette.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB868614899A GB8614899D0 (en) 1986-06-19 1986-06-19 Burette
GB8614899 1986-06-19

Publications (3)

Publication Number Publication Date
EP0250243A2 EP0250243A2 (en) 1987-12-23
EP0250243A3 EP0250243A3 (en) 1988-08-17
EP0250243B1 true EP0250243B1 (en) 1992-02-26

Family

ID=10599692

Family Applications (1)

Application Number Title Priority Date Filing Date
EP87305422A Expired - Lifetime EP0250243B1 (en) 1986-06-19 1987-06-18 Burette

Country Status (5)

Country Link
EP (1) EP0250243B1 (es)
AT (1) ATE72773T1 (es)
DE (1) DE3776818D1 (es)
ES (1) ES2029476T3 (es)
GB (1) GB8614899D0 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0903179A1 (de) 1997-09-18 1999-03-24 Metrohm Ag Mikroventil
DE19906409B4 (de) * 1999-02-16 2006-01-19 Brand Gmbh & Co. Kg Dosiervorrichtung sowie Verfahren zum Betreiben einer Dosiervorrichtung
DE102004003434B4 (de) * 2004-01-21 2006-06-08 Eppendorf Ag Pipettiervorrichtung mit einer Verdrängungseinrichtung und einer damit lösbar verbundenen Antriebseinrichtung
US20200326326A1 (en) * 2019-04-09 2020-10-15 Polymer Technology Systems, Inc. Systems and methods for meter reconfiguration, control, and operation via an insertable chip with microprocessor

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4041995A (en) * 1975-01-30 1977-08-16 Eastman Kodak Company Gas pressure-activated drop dispenser
JPS5919736B2 (ja) * 1981-02-09 1984-05-08 株式会社ニチリヨ− ピペット装置
AU552883B2 (en) * 1981-11-03 1986-06-26 Walter Sarstedt Kunststoff-Spritzgusswerk Blood extracting and centrifuging device
US4671123A (en) * 1984-02-16 1987-06-09 Rainin Instrument Co., Inc. Methods and apparatus for pipetting and/or titrating liquids using a hand held self-contained automated pipette
US4593837A (en) * 1985-03-15 1986-06-10 Eastman Kodak Company Variable volume pipette

Also Published As

Publication number Publication date
DE3776818D1 (de) 1992-04-02
GB8614899D0 (en) 1986-07-23
EP0250243A2 (en) 1987-12-23
ES2029476T3 (es) 1992-08-16
ATE72773T1 (de) 1992-03-15
EP0250243A3 (en) 1988-08-17

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