EP0242445A3 - Dual laser excitation from single laser source - Google Patents

Dual laser excitation from single laser source Download PDF

Info

Publication number
EP0242445A3
EP0242445A3 EP86117397A EP86117397A EP0242445A3 EP 0242445 A3 EP0242445 A3 EP 0242445A3 EP 86117397 A EP86117397 A EP 86117397A EP 86117397 A EP86117397 A EP 86117397A EP 0242445 A3 EP0242445 A3 EP 0242445A3
Authority
EP
European Patent Office
Prior art keywords
laser
light
dual
excitation
laser excitation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP86117397A
Other languages
German (de)
French (fr)
Other versions
EP0242445A2 (en
Inventor
Vernon L. Chupp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of EP0242445A2 publication Critical patent/EP0242445A2/en
Publication of EP0242445A3 publication Critical patent/EP0242445A3/en
Withdrawn legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
    • H01S3/00—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range
    • H01S3/23—Arrangements of two or more lasers not provided for in groups H01S3/02 - H01S3/22, e.g. tandem arrangements of separate active media
    • H01S3/2383—Parallel arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • G01N15/1427—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
    • H01S3/00—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range
    • H01S3/10—Controlling the intensity, frequency, phase, polarisation or direction of the emitted radiation, e.g. switching, gating, modulating or demodulating
    • H01S3/13—Stabilisation of laser output parameters, e.g. frequency or amplitude
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1438—Using two lasers in succession
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497—Particle shape
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
    • G01N21/53—Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
    • H01S2301/00—Functional characteristics
    • H01S2301/08—Generation of pulses with special temporal shape or frequency spectrum
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
    • H01S3/00—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range
    • H01S3/05—Construction or shape of optical resonators; Accommodation of active medium therein; Shape of active medium
    • H01S3/08—Construction or shape of optical resonators or components thereof
    • H01S3/08086—Multiple-wavelength emission
    • H01S3/0809—Two-wavelenghth emission

Landscapes

  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Engineering & Computer Science (AREA)
  • Plasma & Fusion (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Lasers (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A dual laser excitation apparatus comprises a first laser (12) for providing a first beam of light with a plurality of energy lines. A filter or wave­ length disperser (18) is included for dividing the first beam of light into two portions. One of these portions of the light beam is spectrally separated and at least one separated line is directed to a first area of illumination (28). A second laser (29) is positioned to receive the other portion of the divided light beam and is adapted to be energized thereby. The second laser (29) produces a second beam of light directed to a second area of illumination (34). Such a dual laser excitation apparatus is well suited for flow cytometry purposes.
EP86117397A 1986-04-14 1986-12-13 Dual laser excitation from single laser source Withdrawn EP0242445A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/852,007 US4710635A (en) 1986-04-14 1986-04-14 Dual laser excitation from single laser source
US852007 1986-04-14

Publications (2)

Publication Number Publication Date
EP0242445A2 EP0242445A2 (en) 1987-10-28
EP0242445A3 true EP0242445A3 (en) 1989-11-15

Family

ID=25312274

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86117397A Withdrawn EP0242445A3 (en) 1986-04-14 1986-12-13 Dual laser excitation from single laser source

Country Status (3)

Country Link
US (1) US4710635A (en)
EP (1) EP0242445A3 (en)
JP (1) JPS62250360A (en)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8815011D0 (en) * 1988-06-23 1988-07-27 Aubusson R C Programmable line-marking system for multi-purpose sports areas
US5760900A (en) * 1989-03-18 1998-06-02 Canon Kabushiki Kaisha Method and apparatus for optically measuring specimen
US5265172A (en) * 1989-10-13 1993-11-23 Texas Instruments Incorporated Method and apparatus for producing optical flow using multi-spectral images
GB9015793D0 (en) * 1990-07-18 1990-09-05 Medical Res Council Confocal scanning optical microscope
US5127730A (en) * 1990-08-10 1992-07-07 Regents Of The University Of Minnesota Multi-color laser scanning confocal imaging system
US6159686A (en) * 1992-09-14 2000-12-12 Sri International Up-converting reporters for biological and other assays
US5674698A (en) * 1992-09-14 1997-10-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US6399397B1 (en) 1992-09-14 2002-06-04 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US5736410A (en) 1992-09-14 1998-04-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
SE501495C2 (en) * 1993-07-02 1995-02-27 Ericsson Telefon Ab L M Tunable optical device
US5847400A (en) * 1996-02-01 1998-12-08 Molecular Dynamics, Inc. Fluorescence imaging system having reduced background fluorescence
JP4323571B2 (en) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド Optical device
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
WO1999058955A1 (en) * 1998-05-14 1999-11-18 Luminex Corporation Multi-analyte diagnostic system and computer implemented process for same
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
CA2822983C (en) 2000-11-29 2017-05-09 Xy, Llc System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
JP4712212B2 (en) * 2001-03-28 2011-06-29 株式会社トプコン Laser sighting device
US6892464B2 (en) * 2002-03-13 2005-05-17 Kabushiki Kaisha Topcon Laser sighting device
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
JP4595067B2 (en) 2002-08-01 2010-12-08 エックスワイ，エルエルシー Low-pressure sperm cell separation system
CN100570360C (en) 2002-08-15 2009-12-16 Xy公司 A kind of flow cytometer and flow cytometry method
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2305171T3 (en) 2003-03-28 2022-03-21 Inguran Llc Apparatus and methods for providing sexed animal semen
ES2541121T3 (en) 2003-05-15 2015-07-16 Xy, Llc Efficient classification of haploid cells by flow cytometry systems
US20050196826A1 (en) * 2004-03-05 2005-09-08 Sherrill James V. Self calibrating detection
US7892725B2 (en) 2004-03-29 2011-02-22 Inguran, Llc Process for storing a sperm dispersion
AU2005266930B2 (en) 2004-07-22 2010-09-16 Inguran, Llc Process for enriching a population of sperm cells
US7110192B2 (en) * 2005-01-12 2006-09-19 Dako Denmark A/S System and method for a composite lens for a flow cytometer
JP5841315B2 (en) * 2010-04-28 2016-01-13 ソニー株式会社 Fine particle analyzer
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
US9174216B2 (en) 2013-03-13 2015-11-03 DeNovo Science, Inc. System for capturing and analyzing cells
CA2842359A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US10391493B2 (en) 2017-08-29 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
JP7413408B2 (en) 2019-05-07 2024-01-15 バイオ－ラッド ラボラトリーズ インコーポレイテッド Systems and methods for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
CA3143241A1 (en) 2019-06-14 2020-12-17 Bio-Rad Laboratories, Inc. System and method for automated single cell processing and analyses
CN115135986A (en) * 2020-02-19 2022-09-30 贝克顿·迪金森公司 Stroboscopic laser excitation system and method of use
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
EP4314253A2 (en) 2021-03-25 2024-02-07 Iovance Biotherapeutics, Inc. Methods and compositions for t-cell coculture potency assays and use with cell therapy products

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3731110A (en) * 1971-07-12 1973-05-01 Laser system for producing wavelength-tunable optical radiation
US4475236A (en) * 1981-11-12 1984-10-02 Ortho Diagnostic Systems Inc. Method for counting overlapping cell populations in a distribution histogram
EP0121262A2 (en) * 1983-04-05 1984-10-10 Becton Dickinson and Company Method and apparatus for distinguishing multiple subpopulations of cells in a sample

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826364A (en) * 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
US4071298A (en) * 1974-06-27 1978-01-31 Stanford Research Institute Laser Raman/fluorescent device for analyzing airborne particles
US4284412A (en) * 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4609286A (en) * 1984-04-16 1986-09-02 Becton, Dickinson And Company Dispersion prism for separation of wavelengths of spectrally rich light in a flow cytometry apparatus
US4622468A (en) * 1985-07-15 1986-11-11 Sequoia-Turner Corporation Fluorescence intensity compensation method and device

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3731110A (en) * 1971-07-12 1973-05-01 Laser system for producing wavelength-tunable optical radiation
US4475236A (en) * 1981-11-12 1984-10-02 Ortho Diagnostic Systems Inc. Method for counting overlapping cell populations in a distribution histogram
EP0121262A2 (en) * 1983-04-05 1984-10-10 Becton Dickinson and Company Method and apparatus for distinguishing multiple subpopulations of cells in a sample

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
APPLIED PHYSICS LETTERS, vol. 18, no 10, May 15, 1971, pages 433 - 435; L.E. ERIKSON et al.: 'Spectral narrowing of dye laser output by injection of monochromatic radiation into the laser cavity' *
CYTOMETRY, vol. 1, no. 2, 1980, pages 127 - 131, US, D.J. ARNDT-JOVIN et al.: 'A dual laser flow sorter utilizing a CW pumped dye laser *

Also Published As

Publication number Publication date
US4710635A (en) 1987-12-01
EP0242445A2 (en) 1987-10-28
JPH0473748B2 (en) 1992-11-24
JPS62250360A (en) 1987-10-31

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Inventor name: CHUPP, VERNON L.