EP0236305A1 - Vacuum tube for taking blood samples, containing an anticoagulant - Google Patents

Vacuum tube for taking blood samples, containing an anticoagulant

Info

Publication number
EP0236305A1
EP0236305A1 EP19850904694 EP85904694A EP0236305A1 EP 0236305 A1 EP0236305 A1 EP 0236305A1 EP 19850904694 EP19850904694 EP 19850904694 EP 85904694 A EP85904694 A EP 85904694A EP 0236305 A1 EP0236305 A1 EP 0236305A1
Authority
EP
European Patent Office
Prior art keywords
vacuum tube
anticoagulant
blood
blood samples
formaldehyde
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19850904694
Other languages
German (de)
French (fr)
Inventor
Jan Axel Svensson
Nicolaas Wouterus Wilhelmus Hoogervorst
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP0236305A1 publication Critical patent/EP0236305A1/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

Definitions

  • the invention relates to a vacuum tube for taking 5 blood samples, containing an anticoagulant.
  • the usual anticoagulant in such tubes comprises ethylenediaminetetra-acetic acid, EDTA.
  • the tube is used for taking blood samples which are to be used for determining the number of blood cells of different
  • the leucocytes can be saved for a longer time by applying the blood sample to a microscope slide for subsequent analysis, but this requires knowledge and
  • the purpose of the invention is to obtain an extended life of the blood sample taken in a vacuum tube of the kind referred to above, ' and for this purpose the vacuum tube has obtained the characteristics according to claim 1.
  • Vacuum tubes with an anticoagulant comprising EDTA are available on the market with different amounts of anticoagulant and for taking varying amounts of blood.
  • the amount of EDTA can range from 0.054 ml to 0.1 ml and the intended amount of blood which is to be collected in the vacuum tube can range from 3 ml to 10 ml.
  • the EDTA can be replaced by sodium citrate as the coagulant.
  • a certain amount of formaldehyde is added, comprising a water solution containing 30-40% formaldehyde, mixed with distilled water in the ratio 1:30.
  • the amount of diluted- formaldehyde then amounts to 0.005 ml - 0.1 ml, preferably 0.02 ml - 0.06 ml, per ml blood to be collected in the tube.
  • the amount of diluted formaldehyde per ml blood should be at a lower limit at which there is obtained an alkalization of the blood sample, and at an upper limit, at which the blood cells in the blood sample will shrink.
  • the formaldehyde in the vacuum tube can be replaced by calomel (percipitated mercury chlorure) diluted with distilled water in the ratio 1:30 and in an amount within the same limits mentioned for formaldehyde.
  • the blood sample taken in a vacuum tube of the invention is characterized by a longer storing time and an extended temperature durability than blood samples which have been taken in conventional vacuum tubes containing an anticoagulant only. This is illustrated by the accompanying diagram which discloses the number of leucocytes existing in a sample which has been stored at different temperatures with and without the addition of formaldehyde, at different times after the blood sample having been taken.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

Tube à vide pour le prélèvement d'échantillons sanguins, contenant un anti-coagulant et de 0,005 à 0,1 ml par ml de sang de formaldehyde ou de calomel dilué avec de l'eau distillée dans le rapport 1:30.Vacuum tube for taking blood samples, containing an anticoagulant and from 0.005 to 0.1 ml per ml of formaldehyde or calomel blood diluted with distilled water in the ratio 1:30.

Description

VACUUM TUBE FOR TAKING BLOOD SAMPLES, CONTAINING AN
_-a* ANTICOAGULANT
.
The invention relates to a vacuum tube for taking 5 blood samples, containing an anticoagulant.
The usual anticoagulant in such tubes comprises ethylenediaminetetra-acetic acid, EDTA. The tube is used for taking blood samples which are to be used for determining the number of blood cells of different
10 kinds. If the blood is left in the tube for a relative long time (an hour or some hours) at room temperature, the blood cells will change so that measurement cannot be made with the certainty and accuracy which are generally required, by the sample being introduced into
15 an analysis apparatus or a microscope. It is endeavoured to extend the storing time by storing the sample at a reduced temperature, +10 C, and as a consequence thereof the leucocytes can be maintained fairly intact, but this is not true as far as the trombocytes are
20 concerned, which on the contrary are damaged during storing at low temperature.
The leucocytes can be saved for a longer time by applying the blood sample to a microscope slide for subsequent analysis, but this requires knowledge and
25 experience which most often is not to be found with the personnel taking the blood sample, and the consequence thereof is that the sample most often cannot be used for obtaining a reliable result. The alternative is that the sample is handed over to a laboratory in the test tube,
30 but then the time factor mentioned above has to be considered. Thus, it is important to analyse the blood sample which has been taken, without delay, but this may be difficult and, moreover, it is not known how old a sample is and then' it is not possible to adjust the
35 result of the analysis with consideration thereof. The purpose of the invention is to obtain an extended life of the blood sample taken in a vacuum tube of the kind referred to above,' and for this purpose the vacuum tube has obtained the characteristics according to claim 1.
Vacuum tubes with an anticoagulant comprising EDTA are available on the market with different amounts of anticoagulant and for taking varying amounts of blood. Thus, the amount of EDTA can range from 0.054 ml to 0.1 ml and the intended amount of blood which is to be collected in the vacuum tube can range from 3 ml to 10 ml. The EDTA can be replaced by sodium citrate as the coagulant. In the tube of the invention, a certain amount of formaldehyde is added, comprising a water solution containing 30-40% formaldehyde, mixed with distilled water in the ratio 1:30. The amount of diluted- formaldehyde then amounts to 0.005 ml - 0.1 ml, preferably 0.02 ml - 0.06 ml, per ml blood to be collected in the tube. Generally, it can be stated that the amount of diluted formaldehyde per ml blood should be at a lower limit at which there is obtained an alkalization of the blood sample, and at an upper limit, at which the blood cells in the blood sample will shrink. The formaldehyde in the vacuum tube can be replaced by calomel (percipitated mercury chlorure) diluted with distilled water in the ratio 1:30 and in an amount within the same limits mentioned for formaldehyde.
The blood sample taken in a vacuum tube of the invention is characterized by a longer storing time and an extended temperature durability than blood samples which have been taken in conventional vacuum tubes containing an anticoagulant only. This is illustrated by the accompanying diagram which discloses the number of leucocytes existing in a sample which has been stored at different temperatures with and without the addition of formaldehyde, at different times after the blood sample having been taken.

Claims

1. Vacuum tube for taking blood samples, containing an anticoagulant, c h a r a c t e r i z e d in that the vacuum tube also contains 0.005 - 0.1 ml per ml blood of formaldehyde or calomel diluted with distilled water in the ratio 1:30.
2. Vacuum tube as claimed in claim 1, c h a r a c t e r i z e d in that the amount of diluted formaldehyde or calomel, respectively, amounts to 0.02 - 0.06 ml per ml blood.
EP19850904694 1985-09-13 1985-09-13 Vacuum tube for taking blood samples, containing an anticoagulant Pending EP0236305A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SE1985/000349 WO1987001809A1 (en) 1985-09-13 1985-09-13 Vacuum tube for taking blood samples, containing an anticoagulant

Publications (1)

Publication Number Publication Date
EP0236305A1 true EP0236305A1 (en) 1987-09-16

Family

ID=20358895

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19850904694 Pending EP0236305A1 (en) 1985-09-13 1985-09-13 Vacuum tube for taking blood samples, containing an anticoagulant

Country Status (2)

Country Link
EP (1) EP0236305A1 (en)
WO (1) WO1987001809A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1244344A (en) * 1967-11-13 1971-08-25 Abbott Lab Hemagglutination testing procedure employing stabilized and potentiated erythrocytes and a method for their preparation
US4090977A (en) * 1976-11-22 1978-05-23 Research Corporation Osmotically balanced anticoaglant
DK150403C (en) * 1983-07-15 1987-07-13 Radiometer As BLOOD SAMPLING EQUIPMENT

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO8701809A1 *

Also Published As

Publication number Publication date
WO1987001809A1 (en) 1987-03-26

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