EP0184163A3 - Preparation of a multicopy yeast vector - Google Patents

Preparation of a multicopy yeast vector Download PDF

Info

Publication number
EP0184163A3
EP0184163A3 EP85115203A EP85115203A EP0184163A3 EP 0184163 A3 EP0184163 A3 EP 0184163A3 EP 85115203 A EP85115203 A EP 85115203A EP 85115203 A EP85115203 A EP 85115203A EP 0184163 A3 EP0184163 A3 EP 0184163A3
Authority
EP
European Patent Office
Prior art keywords
preparation
yeast vector
vectors
multicopy yeast
multicopy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP85115203A
Other languages
German (de)
French (fr)
Other versions
EP0184163A2 (en
Inventor
Andrzej Dr. Sledziewski
Ewa Dr. Chlebowicz-Sledziewska
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Publication of EP0184163A2 publication Critical patent/EP0184163A2/en
Publication of EP0184163A3 publication Critical patent/EP0184163A3/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Metal Extraction Processes (AREA)
  • Manufacturing Cores, Coils, And Magnets (AREA)
  • Production Of Multi-Layered Print Wiring Board (AREA)

Abstract

Gegenstand der vorliegenden Erfindung ist ein Verfah­ ren zur Herstellung rekombinanter Multi-Kopien-Hefe-­ Vektoren, ein Verfahren zur Vervielfältigung dieser Vektoren, Verwendung der Vektoren sowie die Vektoren selbst. The present invention relates to a method for the production of recombinant multi-copy yeast Vectors, a method of duplicating these vectors, Using the vectors as well as the vectors themselves.

EP85115203A 1984-12-04 1985-11-30 Preparation of a multicopy yeast vector Withdrawn EP0184163A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3444495 1984-12-04
DE19843444495 DE3444495A1 (en) 1984-12-04 1984-12-04 MAKING A MULTI-COPY YEAR VECTOR

Publications (2)

Publication Number Publication Date
EP0184163A2 EP0184163A2 (en) 1986-06-11
EP0184163A3 true EP0184163A3 (en) 1988-01-13

Family

ID=6252051

Family Applications (1)

Application Number Title Priority Date Filing Date
EP85115203A Withdrawn EP0184163A3 (en) 1984-12-04 1985-11-30 Preparation of a multicopy yeast vector

Country Status (15)

Country Link
EP (1) EP0184163A3 (en)
JP (1) JPS61181385A (en)
AU (1) AU5070685A (en)
DE (1) DE3444495A1 (en)
DK (1) DK559585A (en)
ES (3) ES8800342A1 (en)
FI (1) FI854781A (en)
GR (1) GR852891B (en)
HU (1) HU196454B (en)
NO (1) NO854864L (en)
NZ (1) NZ214422A (en)
PT (1) PT81594B (en)
SU (1) SU1364241A3 (en)
YU (1) YU186985A (en)
ZA (1) ZA859240B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3605035A1 (en) * 1986-02-18 1987-08-20 Strahlen Umweltforsch Gmbh PLASMID PWS101, METHOD FOR ITS DETERMINATION AND USE
JP6754697B2 (en) * 2014-12-03 2020-09-16 株式会社カネカ Vectors containing centromere DNA sequences and their uses

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0048081A2 (en) * 1980-09-09 1982-03-24 The Regents Of The University Of California DNA capable of replication and stable mitotic maintenance in a host eukaryote, method of preparation thereof and eukaryotic cell containing same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0048081A2 (en) * 1980-09-09 1982-03-24 The Regents Of The University Of California DNA capable of replication and stable mitotic maintenance in a host eukaryote, method of preparation thereof and eukaryotic cell containing same

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GENE, Band 15, 1981, Seiten 157-166, North-Holland Biomedical Press; C.L. HSIAO et al.: "Characterization of a yeast replication origin (ARS2) and construction of stable minichromosomes containing cloned yeast centromere DNA (CEN3)" *
GENE, Band 39, Nr. 1, 1985, Seiten 25-31, Elsevier Science Publishers B.V., Amsterdam, NL; E. CHLEBOWICZ-SLEDZIEWSKA et al.: "Construction of multicopy yeast plasmids with regulated centromere fuction" *
GENE, Band 39, Nr. 1, 1985, Seiten 25-31, Elsevier Science Publishers B.V., Amsterdam, NL; E. CHLEBOWICZ-SLEDZIEWSKA et al.: "Construction of multicopy yeast plasmids with regulated centromere function" *
TRENDS IN GENETICS, Band 1, 1985, Seiten 8-11, Elsevier Science Publishers B.V., Amsterdam, NL; E.H. BLACKBURN: "Artificial chromosomes in yeast" *

Also Published As

Publication number Publication date
ES8801705A1 (en) 1988-02-16
ES8802075A1 (en) 1988-03-16
NZ214422A (en) 1988-02-12
SU1364241A3 (en) 1987-12-30
EP0184163A2 (en) 1986-06-11
GR852891B (en) 1986-04-03
ES549526A0 (en) 1987-11-01
PT81594B (en) 1987-10-16
ES556975A0 (en) 1988-03-16
DE3444495A1 (en) 1986-06-05
HU196454B (en) 1988-11-28
ES556976A0 (en) 1988-02-16
FI854781A (en) 1986-06-05
NO854864L (en) 1986-06-05
DK559585D0 (en) 1985-12-03
AU5070685A (en) 1986-06-12
YU186985A (en) 1988-06-30
ZA859240B (en) 1987-08-26
DK559585A (en) 1986-06-05
HUT39775A (en) 1986-10-29
ES8800342A1 (en) 1987-11-01
FI854781A0 (en) 1985-12-03
JPS61181385A (en) 1986-08-14
PT81594A (en) 1986-01-01

Similar Documents

Publication Publication Date Title
DE3577430D1 (en) METHOD FOR PRODUCING HIGH CONCENTRATED OXYGEN BY A PRESSURE CIRCUIT PROCESS (P.S.A.).
ES509222A0 (en) A METHOD FOR PRODUCING A RECOMBINANT DNA MOLECULA CHARACTERIZED BY A CODED DNA SEQUENCE OF A CONI- PEPTIDE SIMILAR TO HUMAN INTERFERON.
ATE139994T1 (en) MULTI-STEP PROCESS FOR THE CONTINUOUS PRODUCTION OF ORGANIC POLYISOCYANATES
DE3688336T2 (en) Process for the production of organic acids using microorganisms.
IT8121232A0 (en) PROCEDURE FOR PRODUCING L-GLUTAMIC ACID BY FERMENTATION.
IL87748A (en) Structural gene coding for human serum albumin, its production and its use
EP1027927A3 (en) Copper-based catalysts, process for producing them, their use and method for preparation of alkylhalogenosylanes
ATE14597T1 (en) PROCESS FOR THE ENZYMATIC PRODUCTION OF L-2-AMINO-4-METHYLPHOSPHINOBUTRIC ACID.
ATE30049T1 (en) PROCESS FOR THE PRODUCTION OF D-LACTIC ACID USING LACTOBACILLUS BULGARICUS DSM 2129.
ATE117371T1 (en) SACCHAROMYCES CEREVISIAE STRAIN PRODUCING A HETEROLOGUE PROTEIN AND METHOD FOR PRODUCING THIS HETEROLOGUE PROTEIN BY FERMENTATION OF THIS STRAIN.
EP0184163A3 (en) Preparation of a multicopy yeast vector
DE3484212D1 (en) HYBRID DNA SYNTHESIS OF MATURE GROWTH HORMONE RELEASING FACTOR.
EP0109560A3 (en) Yeast plasmid vector capable of induced expressing and method of using said plasmid
EP0829541A3 (en) Osmo-controlled fermentation process for the preparation of acarbose
IE812346L (en) Preparation of 5-methyl-4-imidazolecarboxylic acid
ATE207139T1 (en) METHOD FOR PRODUCING BUTANETETRACARBONIC ACID
ATE21117T1 (en) FERMENTATION PROCESSES FOR THE PRODUCTION OF ALPHAISOPROPYLACLIC ACID.
ES498451A0 (en) PROCEDURE FOR THE PREPARATION OF A POLYPEPTIDE
EP0348797A3 (en) Application of a carbonyl group containing compound
EP0267605A3 (en) Novel intermediate compounds in a process for producing 16-phenoxy- and 16-substituted phenoxy-9-keto-prostatrienoic acid derivatives
EP0239075A3 (en) Process for producing fish growth hormone polypeptide
ATE29886T1 (en) 7-OXABICYCLOHEPTAN PROSTAGLANDIN INTERMEDIATE PRODUCTS AND PROCESS FOR THEIR PRODUCTION.
EP0796915A3 (en) Process for the preparation and use of acarviosyl-transferase in the conversion of ascarbose-homologous in acarbose and in the preparation of acarbose-homologous
ATE150255T1 (en) METHOD FOR PRODUCING BAKED PRODUCTS
DE3853143D1 (en) Process for the production of AccI restriction endonuclease and methylase.

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE CH DE FR GB IT LI LU NL SE

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): AT BE CH DE FR GB IT LI LU NL SE

17P Request for examination filed

Effective date: 19880621

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 19900531

RIN1 Information on inventor provided before grant (corrected)

Inventor name: SLEDZIEWSKI, ANDRZEJ, DR.

Inventor name: CHLEBOWICZ-SLEDZIEWSKA, EWA, DR.