EP0160091A1 - Production of heterodimeric human fertility hormones - Google Patents
Production of heterodimeric human fertility hormonesInfo
- Publication number
- EP0160091A1 EP0160091A1 EP19850900266 EP85900266A EP0160091A1 EP 0160091 A1 EP0160091 A1 EP 0160091A1 EP 19850900266 EP19850900266 EP 19850900266 EP 85900266 A EP85900266 A EP 85900266A EP 0160091 A1 EP0160091 A1 EP 0160091A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- cell
- hormone
- expression vector
- beta
- alpha
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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Classifications
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4715—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
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- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
- C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
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- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
Definitions
- polypeptide chains have been expressed, via recombinant DNA technology, in host cells such as bacteria, yeast, and cultured mammalian cells. Fiddes,
- the protein is synthesized by a eukaryotic cell and the protein is modified post-translationally, most preferably by
- the EcoRI site..of the plasmid p beta 556 R/B is methylated in a reaction catalyzed by EcoRI methylase, following which the plasmid is cut with Ndel. EcoRI linkers are ligated to
- alpha hCG cDNA in a transcriptional unit controlled by the SV40 early promoter.
- the LH6 plasmid (Fig. 8) is digested with Sau3a and the 390 bp fragment is isolated by polyacrylamide gel electrophoresis. This fragment is then digested
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Protéine sécrétée biologiquement active composée d'une pluralité de sous-unités et préparée par une méthodologie recombinante. Chaque sous-unité est synthétisée par une cellule possédant un vecteur d'expression à réplication autonome, c'est à dire un plasmide ou virus contenant de l'ADN hétérologue assurant le codage de la sous-unité. L'hôte préféré est une cellule eucaryotique. La protéine sécrétée est une hormone telle que l'hCG, l'hormone lutéinisante (LH), l'hormone stimulant le follicule (FSH) ou l'hormone stimulant la thyroïde (TSH).Biologically active secreted protein composed of a plurality of subunits and prepared by a recombinant methodology. Each subunit is synthesized by a cell having an expression vector with autonomous replication, ie a plasmid or virus containing heterologous DNA ensuring the coding of the subunit. The preferred host is a eukaryotic cell. The secreted protein is a hormone such as hCG, luteinizing hormone (LH), follicle stimulating hormone (FSH) or thyroid stimulating hormone (TSH).
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US54821183A | 1983-11-02 | 1983-11-02 | |
US548228 | 1983-11-02 | ||
US06/548,228 US4840896A (en) | 1983-11-02 | 1983-11-02 | Heteropolymeric protein |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0160091A1 true EP0160091A1 (en) | 1985-11-06 |
EP0160091A4 EP0160091A4 (en) | 1987-10-08 |
Family
ID=46229989
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19850900266 Withdrawn EP0160091A4 (en) | 1983-11-02 | 1984-10-31 | Production of heterodimeric human fertility hormones. |
Country Status (1)
Country | Link |
---|---|
EP (1) | EP0160091A4 (en) |
-
1984
- 1984-10-31 EP EP19850900266 patent/EP0160091A4/en not_active Withdrawn
Non-Patent Citations (2)
Title |
---|
CHEMICAL ABSTRACTS, vol. 85, no. 3, 19th July 1976, page 205, abstract no. 15625m, Columbus, Ohio, US; R.C. SHOWNKEEN et al.: "Purification and properties of the subunits of human pituitary follicle-stimulating hormone", & J.ENDOCRINOL. 1976, 69(2), 263-73 * |
See also references of WO8501958A1 * |
Also Published As
Publication number | Publication date |
---|---|
EP0160091A4 (en) | 1987-10-08 |
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