EP0096050A1 - Diagnostic test method. - Google Patents
Diagnostic test method.Info
- Publication number
- EP0096050A1 EP0096050A1 EP82903479A EP82903479A EP0096050A1 EP 0096050 A1 EP0096050 A1 EP 0096050A1 EP 82903479 A EP82903479 A EP 82903479A EP 82903479 A EP82903479 A EP 82903479A EP 0096050 A1 EP0096050 A1 EP 0096050A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- test tube
- test
- antigen
- antibody
- quarter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N33/555—Red blood cell
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/81—Tube, bottle, or dipstick
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
- Y10S436/818—Human chorionic gonadotropin
Definitions
- the invention relates to a . method for qualita ⁇ tively or semi-quantitatively detecting the presence of an antigen or antibody in a test liquid, and also to a test kit which permits carrying out such a method.
- the diagnosis of pathological conditions in man or animals is frequently carried out by using immuno ⁇ chemical reactions.
- the presence of an antigen or antibody in a body fluid is determined i munologically by bringing into contact the corresponding antibody or antigen with the particular body fluid to be tested, usually blood serum or urine.
- the presence of the antigen or antibody to be detected can then be noted by the fact that a less soluble antigen-antibody complex is formed.
- erythrocytes erythrocytes
- bacterial cells erythrocytes
- bentonite latex particles
- cellulose cellulose
- an antigen in a test sample or test liquid now be demonstrated by bringing the test sample into contact with anti ⁇ bodies against this antigen, which are coupled to erythrocytes. If the antigen actually is present in the test sample, an immunochemical reaction will take place between the particular antigen and the antibody which is coupled to erythrocytes, resulting in an antigen-antibody complex coupled to erythrocytes and in a coupling together (agglutination) of such erythrocyte particles; on settling out in a test tube having a round bottom, these agglutinated erythrocytes give a plain light brown pattern.
- the antigen is not present in the test liquid, no immunochemical reaction, hence also no agglutination, can take place.
- the antibody bonded to erythrocytes will settle out after a short time and give a characteristic ring pattern in a test tube having a round bottom.
- obtaining a characteristic ring pattern in the test tube therefore means that the suspected antigen is not present in the test liquid, and therefore that the test result is negative.
- the antigen in question can also be detected in a different manner, namely by bringing the test sample into contact with 2 reagents: (1) the antibody against the particular antigen and (2) the particular antigen coupled to erythrocytes.
- this antigen will react directly with the antibody, giving a (soluble) antigen-antibody complex, while the unused erythrocyte-coupled antigen will
- OMPI settle out after a short time and, in a test tube with a round bottom, lead to the formation of a characteristic ring pattern.
- the interpretation of the test result therefore depends on the chosen test set-up, the characteristic ring pattern of the erythrocyte- bonded antigen or antibody each time forms the basis of the test method.
- test tube having a bottom in the shape of an inverted prism or truncated prism, such that the lower edge of the tube forms a strip having a length which is at least equal to one-quarter of the distance between two opposite walls or where appropriate to a quarter of the diameter of the test tube, (a) assists the readability of the test result, so that significantly fewer incorrect observations are made and (b) increases the sensitivity of the test by a factor of 2 to 3; this means that the antigen or anti- body can new be detected in lower concentrations than is possible with a conventional round- bottomed test tube.
- the use of the test tube according to the invention can moreover have the optical advantage that the imm ⁇ nological test can be set up in such a
- the depth of the inverted prism is not particularly critical; however a preferred "depth" is approximately equal to 25-75% of the diameter of the test tube and more especially 40-50% of the diameter of the test tube.
- test method according to the invention can be employed for various types of diagnostic test purposes, for example for the qualitative detection of pathological antibodies in blood or urine, but also for the detection of HCG in urine, as a result of which the test method is particularly suitable as a pregnancy test.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Endocrinology (AREA)
- Mycology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Reproductive Health (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Procédé d'exécution de réaction immuno-chimique dans un tube de test dont le fond possède la forme d'un prisme renversé ou tronqué, de sorte que le bord inférieur du tube forme une bande de longueur au moins égale à un quart de la distance entre les deux parois opposées, ou, le cas échéant, à un quart du diamètre du tube de test, ce qui se traduit par une amélioration aussi bien de la sensibilité que de la lisibilité.Method for carrying out an immuno-chemical reaction in a test tube, the bottom of which has the shape of an inverted or truncated prism, so that the lower edge of the tube forms a strip of length at least equal to a quarter of the distance between the two opposite walls, or, if necessary, at a quarter of the diameter of the test tube, which results in an improvement in both sensitivity and readability.
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NL8105341 | 1981-11-26 | ||
NL8105341A NL8105341A (en) | 1981-11-26 | 1981-11-26 | DIAGNOSTIC TEST METHOD. |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0096050A1 true EP0096050A1 (en) | 1983-12-21 |
EP0096050B1 EP0096050B1 (en) | 1986-02-26 |
Family
ID=19838449
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP82903479A Expired EP0096050B1 (en) | 1981-11-26 | 1982-11-24 | Diagnostic test method |
Country Status (7)
Country | Link |
---|---|
US (1) | US4628036A (en) |
EP (1) | EP0096050B1 (en) |
BE (1) | BE895122A (en) |
DE (1) | DE3269493D1 (en) |
DK (1) | DK156926C (en) |
NL (1) | NL8105341A (en) |
WO (1) | WO1983002009A1 (en) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5186898A (en) * | 1984-03-23 | 1993-02-16 | Applied Biosystems, Inc. | Automated polypeptide synthesis apparatus |
US5501949A (en) * | 1985-12-10 | 1996-03-26 | Murex Diagnostics Corporation | Particle bound binding component immunoassay |
US4789628A (en) * | 1986-06-16 | 1988-12-06 | Vxr, Inc. | Devices for carrying out ligand/anti-ligand assays, methods of using such devices and diagnostic reagents and kits incorporating such devices |
GB8724549D0 (en) * | 1987-10-20 | 1987-11-25 | Coombs R R A | Immunoassay |
US4995967A (en) * | 1987-11-06 | 1991-02-26 | Akzo N.V. | Separator for cell-containing liquids |
US5017342A (en) * | 1988-06-01 | 1991-05-21 | Ortho Diagnostic Systems Inc. | Device for immunoassay determinations |
US5620845A (en) * | 1988-06-06 | 1997-04-15 | Ampcor, Inc. | Immunoassay diagnostic kit |
IT1246993B (en) * | 1991-01-10 | 1994-12-12 | Diesse Diagnostica | TEST TUBE FOR BIOLOGICAL ANALYSIS EQUIPPED WITH CONTROL DEVICE, EFFICIENCY AND POSITION, FOR PHOTOMETRIC READINGS. |
DE59206639D1 (en) * | 1991-05-07 | 1996-08-01 | Hoffmann La Roche | Cuvette for performing optical measurements |
US5310527A (en) * | 1992-12-14 | 1994-05-10 | E. I. Du Pont De Nemours And Company | Tube for use in a pelleting centrifuge rotor |
WO1996001693A1 (en) * | 1994-07-11 | 1996-01-25 | Akzo Nobel N.V. | Micro sample tube with reduced dead volume and bar code capability |
JP3251441B2 (en) * | 1994-09-30 | 2002-01-28 | シスメックス株式会社 | Cuvette and cuvette transporter |
US5958349A (en) * | 1997-02-28 | 1999-09-28 | Cepheid | Reaction vessel for heat-exchanging chemical processes |
US20050148063A1 (en) * | 2003-12-24 | 2005-07-07 | Cracauer Raymond F. | Disposable reaction vessel with integrated optical elements |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2213711A5 (en) * | 1973-01-08 | 1974-08-02 | Prugnaud Robert | Diagnostic test for pregnancy - by identification of high level of chorionic gonadotrophin in urine |
CH588700A5 (en) * | 1975-02-28 | 1977-06-15 | Hoffmann La Roche | |
FI56905C (en) * | 1978-02-28 | 1980-04-10 | Osmo A Suovaniemi | FOERFARANDE OCH ANORDNING FOER AUTOMATISK MAETNING AV AGGLUTINATIONSPROV T EX I SPEKTROPOTOMETER ADSOPTIONSFOTOMETER FLUOROMETER ELLER NEFELOMETER |
FR2418463A1 (en) * | 1978-02-28 | 1979-09-21 | Nal Transfusion Sanguine Centr | PROCESS FOR THE SCREENING OR IDENTIFICATION, IN A BIOLOGICAL ENVIRONMENT, OF VIRAL ANTIGENS, ERYTHROCYTAL OR CELLULAR ANTIGENS OR ANTIBODIES |
JPS561352A (en) * | 1979-06-20 | 1981-01-09 | Olympus Optical Co Ltd | Container for corpuscular cohesion judgement |
JPS5766361A (en) * | 1980-10-09 | 1982-04-22 | Olympus Optical Co Ltd | Plate-shaped apparatus for judging cohesion of particle |
US4427634A (en) * | 1982-03-12 | 1984-01-24 | Wright Laboratories, Inc. | Apparatus for microscopic examination of specimens |
-
1981
- 1981-11-26 NL NL8105341A patent/NL8105341A/en not_active Application Discontinuation
-
1982
- 1982-11-24 BE BE0/209553A patent/BE895122A/en not_active IP Right Cessation
- 1982-11-24 US US06/518,801 patent/US4628036A/en not_active Expired - Fee Related
- 1982-11-24 EP EP82903479A patent/EP0096050B1/en not_active Expired
- 1982-11-24 WO PCT/NL1982/000043 patent/WO1983002009A1/en active IP Right Grant
- 1982-11-24 DE DE8282903479T patent/DE3269493D1/en not_active Expired
-
1983
- 1983-07-05 DK DK311483A patent/DK156926C/en not_active IP Right Cessation
Non-Patent Citations (1)
Title |
---|
See references of WO8302009A1 * |
Also Published As
Publication number | Publication date |
---|---|
EP0096050B1 (en) | 1986-02-26 |
NL8105341A (en) | 1983-06-16 |
DK156926C (en) | 1990-03-12 |
WO1983002009A1 (en) | 1983-06-09 |
DK311483A (en) | 1983-07-05 |
DK311483D0 (en) | 1983-07-05 |
DK156926B (en) | 1989-10-16 |
US4628036A (en) | 1986-12-09 |
BE895122A (en) | 1983-05-24 |
DE3269493D1 (en) | 1986-04-03 |
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Legal Events
Date | Code | Title | Description |
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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AK | Designated contracting states |
Designated state(s): DE FR GB |
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17P | Request for examination filed |
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RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: THEUNISSEN, HENDRIKUS JOHANNES JOSEPHUS Inventor name: VAN HELL, HANS Inventor name: SCHEEPENS, ANTONIUS HENDRIKUS JOHANNES MARIA |
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