EP0000952A1

EP0000952A1 - Benzhydryl guanidine derivatives and pharmaceutical compositions

Info

Publication number: EP0000952A1
Application number: EP78100774A
Authority: EP
Inventors: Chris Royce Rasmussen
Original assignee: McNeilab Inc; McNeil Laboratories Inc
Current assignee: Janssen Pharmaceuticals Inc
Priority date: 1977-08-29
Filing date: 1978-08-28
Publication date: 1979-03-07
Also published as: ZA784885B; EP0000952B1; JPS5446754A; NZ188178A; DE2860474D1; AU3915878A; US4161541A; AU519174B2; US4101659A

Abstract

The invention relates to benzhydryl guanidine derivatives having the formula as defined in claim 1 and pharmaceutical compositions having hypoglycemic activity, containing such benzhydryl guanidine derivatives.

Description

In U.S. Patent No. 3,961,056, certain benzyl derivatives of guanidine are described as having anti-arrhythmic and diuretic uses. However, no benzhydryl derivatives of guanidine are described.
This invention relates to benzhydryl guanidine derivatives having the formula I
wherein:

R₁ is a member selected from the group consisting of hydrogen and loweralkyl, preferably methyl and ethyl;
R₂ is a member selected from the group consisting of hydrogen and loweralkyl, preferably methyl and ethyl;
R₃ is a member selected from the group consisting of hydrogen, loweralkyl, preferably methyl and ethyl, and cycloalkyl, preferably cyclopentyl and cyclohexyl;
taken together may represent a member selected from the group consisting of
and
Y and Z are each a member selected from the group consisting of hydrogen, halo, loweralkyl, preferably methyl, and loweralkyloxy, preferably methoxy and ethoxy.

The novel benzhydryl guanidine derivatives of Formula (I) as free bases are generally soluble in many common polar and non-polar organic solvents such as aromatic hydrocarbons, e.g., benzene, toluene, and the like; haloaromatic hydrocarbons, e.g., chlorobenzene, 1,2-dichlorobenzene, and the like; haloaliphatic hydrocarbons, e.g., chloroform, methylene dichloride, 1,2-dichlorethane and the like; lower alkanols, e.g., methanol, isopropanol, t-butanol and the like, ethers, e.g., diethyl ether, dioxane and the like, and ketones, e.g., acetone, 2-butanone and the like. They are preferably obtained and employed in the form of their acid addition salts which are generally white crystalline solids soluble in water and polar solvents such as the lower alkanols, ketones and the like.
The non-toxic, therapeutically acceptable acid addition salts of the Formula (I) compounds are also embraced within the scope of this invention. Suitable acids may be inorganic acids such as hydrochloric, hydrobromic, hydroiodic, phosphoric, nitric and the like acids, or organic acids such as acetic, propionic, glycolic, pamoic, pyruvic, malonic, succinic, maleic, fumaric, malic, tartaric, citric, benzoic, cinnamic, mandelic, methanesulfonic, ethanesulfonic, benzenesulfonic, p-toluenesulfonic, cyclohexanesulfamic, salicylic , p-aminosalicylic and the like acids. The preferred acid addition salts are the hydrohalic addition salts.
As used herein, the term "loweralkyl" refers to a straight or branch chained hydrocarbon radical having from 1 to 5 carbons, such as, for example, methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, pentyl, isoamyl and the like; and the term "halo" represents a halogen of atomic weight less than 127, i.e., chloro, bromo, fluoro and iodo.
The compounds of Formula (I) are conveniently prepared by reacting a methylthio compound of Formula (II), wherein R₁, Y and Z are as previously defined, in acid addition salt (HX) form with an appropriate amine of Formula (III), wherein R₂, R₃ and -NR₂R₃ are as previously defined, preferably utilizing a stoichiometric excess of (III), in a lower alkanol solvent such as isopropanol and t-butanol and generally at reflux temperatures to yield the benzhydryl guanidine compounds of Formula (I) in acid addition salt form which are readily transformed into the corresponding base form by conventional treatment with suitable alkali. Alternatively, the reaction of (II) with (III) may be performed utilizing approximately equimolar amounts in wnich case up to an equimolar amount of a suitable tertiary amine, such as, for example, triethylamine, tripropylamine and the like, is preferably added in order to enhance the rate of reaction. The foregoing reaction mav be illustrated as follows:
The precursors of Formula (II) are obtainable by methodologies reported in the literature. For example, the compound wherein Y, Z and R₁ all equal hydrogen may be prepared according to S. 0. Winthrop et al., J. Am. Chem. Soc., 79, 3496 (1957), which describes the reaction of benzhydryl amine, preferably as the hydrochloride salt, with ammonium thiocyanate to yield N-benzhydryl thiourea which is then methylated by standard S-methylation techniques to yield methyl N-benzhydrylcarbamimidothioate as an acid addition salt.
Alternatively, the precursors of Formula (II) may be prepared by the following synthetic sequence. The benzhydrylamines of Formula (IV), which compounds and methods of preparing same are known in the literature, are transformed into the corresponding benzhydryl isothio- cyanates of Formula (V) according to the method described by J. C. Jochims et al., Angew. Chem. Internat. Ed., 6 (2), 174 (1967), which method involves the interaction of (IV) with excess carbon disulfide in the presence of an equimolar amount of dicyclohexylcarbodiimide (DCC) at initial temperatures preferably below 0°C in anhydrous ether.
The resultant-benzhydryl isothiocyanate (V) , which may be isolated from the reaction mixture and purified by conventional means, is then converted to the corresponding N-benzhydrylthiourea of Formula (VI) by the reactions of (V) with ammonia or a primary amine of the formula, R₁ NH₂, wherein Ri is a substituent as previously defined, preferably in an ethereal solvent such as, for example, diethyl ether, tetrahydrofuran (THF),.dioxane, 1,2-dimethoxyethane and the like at about 0°C to ambient temperature.
The thus-obtained N-benzhydrylthiourea (VI), which may be isolated from the reaction mixture and purified by conventional techniques, is then subjected to S-methylation according to methodologies reported in the literature for the conversion of thioureae to S-methyl- pseudothioureas, e.g., see Winthrop et al., loc. cit., which utilize methyl iodide and methyl chloride as the methylating agent. Other methylating agents which may be employed include methyl mesylate, methyl tosylate, methyl fluorosulfonate and the like. The preferred methylating agent is methyl iodide. In general, the S-methylated product (II) is obtained in the form of an acid addition salt (HX).
The foregoing synthetic sequence may be illustrated by the following flow diagram in which the heretofore-described benzhydryl moiety is represented by the letter "A".
Typical benzhydryl guanidine derivatives of Formula (I) which may be prepared according to the synthetic procedures described herein by using appropriate precursors are:

N-(4,4'-dichlorobenzhydryl)-4-thiamorpholinecarboximidamide;
N-(4,4'-dichlorobenzhydryl)-N',N'-diethylguanidine;
N-(4-chlorobenzhydryl)-N'-cyclohexyl-N'-methylguanidine;
N-(4-chlorobenzhydryl)-N'-ethyl-N',N"-dimethylguanidine;
N-(4,4'-diethoxybenzhydryl)-N'-ethyl-l-piperidinecarbox- imidamide;
N-(4,4'-diethoxybenzhydryl)-N'-ethyl-l-pyrrolidinecarbox- imidamide;
N-(4,4'-dimethoxybenzhydryl)-N'-cyclopentyl-N'-methylguanidine;
N-(4,4^1-dimethoxybenzhydryl)-4-morpholinecarboximidamide;
N-(4,4'-dimethoxybenzhydryl)-l-(4-phenylpiperazine) carboximidamide;
N-(4,4^1-dibromobenzhydryl)-l-piperidinecarboximidamide;
N-(3-bromobenzhydryl)-4-thiamorpholinecarboximidamide;
N-(3-bromobenzhydryl)-N'-methyl-4-thiamorpholinecarbox- imidamide;
N-(4-chlorobenzhydryl)-1-(4-methylpiperazine)carboximidamide;
N-(4-chlorobenzhydryl)-N'-cyclohexyl-N'-methylguanidine;
N-(4,4'-diethoxybenzhydryl)-N',N'-diethylguanidine;
N-(4,4'-diethoxybenzhydryl)-1-pyrrolidinecarboximidamide;
N-(4,4'-dimethylbenzhydryl)-4-morpholinecarboximidamide;
N-(4,4'-dimethylbenzhydryl)-N',N'-diethylguanidine;
N-(4-methylbenzhydryl)-N'-cyclohexyl-N'-methylguanidine;
N-(4-methylbenzhydryl)-N'-ethyl-N'-methylguanidine;
N-(4-methoxybenzhydryl)-N'-cyclopentyl-N'-methylguanidine;
_N-(4-methoxybenzhydryl)-l-pyrrolidinecarboximidamide; and
N-(4-methoxybenzhydryl)-4-morpholinecarboximidamide.

In view of their structure, the compounds of Formula (I) are inherently capable of existing in tautometric forms (I-a) and (I-b):
Furthermore, when the benzhydryl substituents Y and Z are non-identical or in different positions on their respective phenyl rings, it is evident that optical isomeric forms (d- and l-) of the Formula (I) products are possible. For example, by utilizing an appropriate resolved (d- or l-) benzhydrylamine of Formula (IV) as a precursor in the synthetic sequence previously described, the final Formula (I) product thus-obtained will similarly be a d- or 1-optical isomer.
In the following examples, the terms "benzhydryl" and "diphenylmethyl" are equivalent. Furthermore, the terms "guanidine" and "N-carboximidamide" are utilized as dictated for purposes of clarity according to the structure of the particular compound of Formula (I) under consideration.
The compounds represented by Formula (I) and the acid addition salts thereof are useful as hypoglycemic agents suitable for lowering blood sugar. This property may be demonstrated by the rat glucose tolerance test, an extremely sensitive standard procedure used in the diagnosis of diabetes and hypoglycemic disease states.
In this test, male Sprague-Dawley rats (Charles River 184-250 grams) are given water ad libitum and fasted 24 hours prior to the experiment. Two to five rats are used for each test and control group. Test compounds, 0.5-100 mg./kg., are administered (s.c., i.p. or orally) suspended in 0.5 or 1.0 milliliter, but preferably the former, of 0.5-1.0% methylcellulose vehicle. Control animals are given an equal amount of vehicle. Serial blood samples (0.1 milliliter) are obtained from the tail without anesthesia prior to and at 30, 60, 90, 120, 150 and 180 minutes after administration of 0.8 to 1.0 gram of glucose per kilogram of body weight in 1 milliliter of water. (The glucose is given orally if the test compound has been given parenterally, and subcutaneously if the test compound has been given orally.) Specimens of blood are immediately deproteinized with aqueous solutions of Ba(OH)₂ and Z_NSO₄ and glucose levels are determined using the glucose oxidase assay described by L.P. Cawley et al., "Ultra Micro Chemical Analysis of Blood Glucose with Glucose Oxidase", Amer. J. Clin. Path., 32, 195 (1959). The blood glucose values at each time point are expressed in terms of milligram percent (mg. glucose/100 ml. of blood). The mean glucose values of the controls are compared statistically by the Student's t-Test to the means of the experimental group at each of the corresponding time points. If the compound lowers the blood glucose significantly at any time at a 95% confidence limit, the compound is considered to have hypoglycemic activity. The blood glucose lowering, expressed as percent lowering, is obtained by dividing the difference between the mean blood glucose values for test and control animals by the mean glucose value for the control animal.
For reducing blood glucose, the compounds of Formula (I) may be employed at a dosage range of about 0.5-100 mg./kg. body weight. It has been found, for example, that administration of the most preferred compounds, the acid addition salts of N-benzhydryl-l-pyrrolidinecarboximidamide or N-benzhydryl-4-morpholine- carboximidamide, at 1-10 mg./kg. body weight p.o. provides a marked lowering of blood sugar in test animals.
In view of the aforementioned hypoglycemic activity of the Formula (I) compounds and salts thereof, this invention provides valuable pharmaceutical compositions comprising the said compounds as the active hypoglycemic ingredient in admixture with a pharmaceutical carrier.
To prepare the pharmaceutical compositions of this invention, a benzhydryl guanidine of Formula (I) or acid addition salt thereof, as the active hypoglycemic ingredient, is intimately admixed with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques, which carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral. In preparing the compositions in oral dosage form, any of the usual pharmaceutical media may be employed. Thus, for liquid oral preparations, such as, for example, suspensions, elixirs and solutions, suitable carriers and additives include water, glycols,oils, alcohols, flavoring agents, preservatives, coloring agents and the like; for solid oral preparations such as, for example, powders, capsules and tablets, suitable carriers and additives include starches, sugars, diluents, granulating agents, lubricants, binders, disintegrating agents and the like. Because of their ease in administration, tablets and capsules represent the most advantageous oral dosage unit form, in which case solid pharmaceutical carriers are obviously employed. If desired, tablets may be sugar coated or enteric coated by standard techniques. For parenterals, the carrier will usually comprise sterile water, though other ingredients, for example, for purposes such as aiding solubility or for preservation, may be included. Injectable suspensions may also be prepared, in which case appropriate liquid carriers, suspending agents and the like may be employed. The pharmaceuctical compositions herein will contain, per dosage unit, e.g., tablet, capsule, powder, injection, teaspoonful and the like, from about 10 to about 500 mg. of the active ingredient, and, preferably, from about 10 to about 250 mg.
Following are specific examples of compounds of Formula (I) which can be compounded into pharmaceutical compositions and used within the scope of this invention. These examples are not intended to be limitations upon the broad scope of the invention but merely illustrative.

EXAMPLE I

A. N-Diphenylmethyl-1-pyrrolidinecarboximidamide Hydroiodide:

To 76.86 g (0.2 mole) of methyl-N-(diphenylmethyl)-carbami- midothioate hydroiodide in t-BuOH (120 ml) is added 28.44 g (0.4 mole) of pyrrolidine. The resulting mixture is allowed to heat under reflux on the steam bath for 3-1/2 hours. The reaction mixture is cooled to room temperature affording crystals of crude HI salt, mp. 200-203°C. Recrystallization from tert-BuOH gives pure N-diphenylmethyl-1-pyrrolidine- carboximidamide hydroiodide; mp. 205-206°C.

B. N-Diphenylmethyl-1-pyrrolidinecarboximidamide Hydrochloride Hemihydrate:

Conversion of the HI salt of Example IA to the free base in _CH2C1₂ by treatment with cold 20% NaOH; drying the organic layer over K₂CO₃, filtration, and solvent removal in vacuo gives the free base.
Treatment of the free base in moist isopropanol with HC1 gas furnishes the crude hydrochloride hemihydrate. The crystals are recrystallized from i-PrOH to give the pure salt; N-diphenylmethyl-1-pyrrolidinecarboximidamide hydrochloride hemihydrate; mp. 230-233°C.

EXAMPLE II

N-(Diphenylmethyl)-4-morpholinecarboximidamide:

1-(Diphenylmethyl)-2-methyl-2-thiopseudourea hydroiodide (19.2 g, 0.05 mole) in 75 ml tert-butanol is heated at reflux for 24 hours with morpholine (8.7 g, 0.1 mole) under a slow stream of nitrogen. The effluent gas is passed through sodium hypochlorite and sodium hydroxide traps to remove the methyl mercaptan formed in the reaction. The mixture was taken to dryness in vacuo and the residue was treated with 3N sodium hydroxide. Extraction with methylene chloride, washing the combined extracts with water, drying over potassium carbonate, filtration and solvent removal under reduced pressure furnishes an oil which solidifies on standing. Recrystallization several times from acetone-ether gives N-(diphenylmethyl)-4-morpholinecarboximidamide as a white solid; mp. 122-124°C.

EXAMPLE III

N,N-Diethyl-N'-diphenylmethylguanidine Hydroiodide:

To asuspension of 9.61 g (0.025 mole) of methyl-N-(diphenylmethyl)-carbamimidothioate hydroiodide in 20 ml of t-BuOH is added 3.66 g (0.05 mole) of diethylamine. The mixture is heated under reflux for twenty hours (trapping the methyl mercaptan formed in the reaction with NaOH and NaOCl sol'n). About two additional mls of diethyl amine is added and refluxing is resumed for another 4 hours. The resulting white solid is filtered from the cooled reaction mixture and washed with t-BuOH and ether to yield crude product mp. 187-189°C. The pure product, N,N-diethyl-N-diphenylmethylguanidine hydroiodide, is isolated after one recrystallization from 1:1:1 MeOH/i-PrOH/t-BuOH as a white crystalline solid, mp. 187-189°C.

EXAMPLE IV

N-(Diphenylmethyl)-1-piperidinecarboximidamide Monohydroiodide:

To a suspension of 9.61 g (0.025 mole) of methyl-N-(diphenylmethyl)-carbamimidothioate hydroiodide in 20 ml of t-BuOH is added 4.26 g (0.05 mole) of piperidine. The mixture is heated under reflux overnight. The resulting crystals after cooling in ice, are filtered; mp. 200-213°C. One recrystallization from methanol-t-BuOH yields the pure product, N-(diphenylmethyl)-1-piperidinecarbox- imidamide monohydroiodide as a white crystalline solid; mp. 207-210°C.

EXAMPLE V

N-(Diphenylmethyl)-1-(4-methyl)-piperazinecarboximidamide Monohydroiodide Hydrate:

To 8.84 g (0.023 mole) of methyl-N-diphenylmethylcarbami- midothioate hydroiodide in t-BuOH is added 4.71 g (0.047 mole) of N-methylpiperazine. The resultant mixture is allowed to heat under reflux overnight. The mixture is evaporated to dryness in vacuo then diluted with i-PrOH to give crystals; mp. 200-204°C. The crystals are recrystallized from 2-propanol affording pure product, N-(diphenylmethyl)-1-(4-methyl) piperazinecarbox- imidamide monohydroiodide hydrate; mp. 202-204°C.

EXAMPLE. VI

A. p-Chlorobenzhydryl Isothiocyanate:

A mixture of 80 ml of carbon disulfide and 39.82 g (0.193 mole) of dicyclohexylcarbodiimide in 100 ml of anhydrous ether, under dry N₂, is cooled with stirring to -35°C. Then 42.23 g (0.194 mole) of p-chlorobenzhydrylamine in 500 ml of dry ether is added over about 5 min such that the temperature within the reaction vessel does not rise above -20°C. The temperature is allowed to rise slowly over 3 hrs. to ca. 25°C and stirring is continued overnight. Dicyclohexylthiourea was removed by filtration and the filtrate is taken to dryness in vacuo. The cloudy oil is treated with 500 ml of pentane which dissolves the product and precipitates more insoluble impurities. Filtration through filter aid (diatomaceous earth) and solvent removal in vacuo gives p-chlorobenzhydryl isothiocyanate as an oil; IR (neat) .2140 cm^-1.

B. N-(4-Chlorobenzhydryl)thiourea:

A solution of 4-chlorobenzhydryl isothiocyanate (5.2 g, 0.02M) in 50 ml of dry ether at 0°C is treated with NH3 (anhyd.) for 1/2 hour while maintaining a temperature of 0°C with stirring. Stirring is continued an additional 1.5 hours at 9 to 10°C during which time a white solid appears. The crude thiourea is filtered and washed thoroughly with ether; mp. 173-175°C. The filtrate is concentrated in vacuo to yield additional product. The combined crops of N-(4-chlorobenzhydryl)thiourea are used in the next step without further purification.

C. Methyl N-(4-chlorobenzhyoryl)-carbamimidothioate Hydroiodide:

A solution of 5.3 g (0.019 mole) of N-(4-chlorobenzhydryl)thiourea in 25 ml of methanol is treated with 2.64 g (0.019 mole) of methyl iodide and allowed to stir at room tomperature overnight. The methanol is removed in vacuo to yield the crude pseudothiourea as an oil. The oily methyl N-(4-chlorobenzhydryl)-carbamimidothioate hydroiodide is used in the next step without further purification.

D. N-(4-Chlorobehzhydryl)-1-pyrrolidinecarboximidamide Hydroiodide:

A mixture of 7.0 g (0.017 mole) of methyl n-(4-chlorobenzhydryl)carbamimidothioate hydroiodide and 2.85 g (0.04 mole) of pyrrolidine in 20 ml of t-BuOH is heated at reflux overnight. The t-BuOH is removed in vacuo and the crude guanidine hydroiodide is crystallized from methanol-ether. The crude product is recrystallized from methanol-t-butanol to yield pure N-(4-chlorobenzhydryl)-1-pyrrolidinecarboximidamide hydroiodide; mp. 218-220°C (dec).

EXAMPLE VII

N-Cyclopentyl n-methyl-N'-(diphenylmethyl)guanidine:

To a suspension of 9.61 g (0.025 mole) of methyl N-(diphenylmethyl)carbamimidothioate hydroiodide in 20 ml of t-BuOH is added 3.5 g (0.035 mole) of N-methylcyclopentyl- amine and 4 ml of triethylamine. The mixture is then heated under reflux overnight. Upon cooling,the crude guanidine hydroiodide forms as an oil that does not crystallize under various conditions. The free base is liberated with 10% NaOH and extracted with CH₂Cl₂. The methylene chloride sol'n is dried (R₂CO₃) and concentrated to dryness in vacuo to yield 8.0 g of an oil. The free base crystallizes from ether to give N-cyclopentyl-N-methyl-N'-(diphenylmethyl)guanidine; mp. 102-104°C.

EXAMPLE VIII

N-Diphenylmethyl-4-phenyl-1-piperazinecarboximidamide Monohydroiodide Hemihydrate

A mixture of 6.38 g (0.017 mole) of N-benzhydryl-S-methyl- pseudothiourea monohydroiodide and 5..81 g (0.034 mole) of N-phenylpiperazine in t-BuOH is heated under reflux overnight. About 50 ml of moist 2-propanol is added and the mixture is then allowed to crystallize. The crude guanidine melts at 119-128°C. Recrystallization from 1:2 MeOH/t-BuOH and then from 2-propanol gives pure N-diphenylmethyl-4-phenyl-l-piperazinecarboximidamide monohydroiodide hemihydrate; mp. (113-117) 208-210°C.

EXAMPLE IX

A. N-Benzhydryl-N'-methylthiourea:

A solution of 13.50 g (0.06 mole) of benzhydrylisothio- cyanate in ether is saturated with anhydrous methylamine in an ice bath. The crystals are filtered to yield the product, N-benzhydryl-N'-methylthiourea; mp. 152-154°C, which is sufficiently pure for the next step.

B. Methyl N-Diphenylmethyl-N'-methylcarbamimidothioate Hydroiodide:

A suspension of 14.64 g(0.057 mole) of N-benzyl-N'-methyl- thiourea in methanol is treated with 8.09 g (0.057 mole) of methyl iodide and allowed to stir overnight. The solvent is removed in vacuo and the residue dissolved in t-BuOH and 2-propanol. Cooling and scratching yields methyl N-diphenylmethyl-N'-methylcarbamimidothioate hydroiodide; mp. 170-173°C.

C. N-(Diphenylmethyl)-N'-methyl-1-pyrrolidinecarboximid- amide Hydroiodide Hydrate:

A mixture of 20.81 g (0.052 mole) of the compound of Example IX B and 7.4 g (0.104 mole) of pyrrolidine in t-BuOH is heated at reflux overnight. Crystals started forming after about two hours of heating. The mixture is filtered to yield crude guanidine; mp. 200-205°C. Recrystallization from t-BuOH yields N-(diphenylmethyl)-N'- methyl-1-pyrrolidinecarboximidamide hydroiodide hydrate; mp. 211.5-213.5°C.

EXAMPLE X

A. 4-Methylbenzhydrylisothiocyanate:

A solution of 20.42 g (0.099 mole) of N,_N-dicyclohexylcarbodiimide and 40 ml of carbon disulfide in 50 ml of dry Et₂0 at -40°C under N₂ is treated dropwise with stirring over a 5 min period (so that temperature does not exceed -30°C) with a solution of 19.7 g (0.1 mole) of 4-methylbenzhydrylamine. The temperature is allowed to rise slowly over 3 hours to ca. 25°C and stirring is continued overnight. The insoluble dicyclohexylthiourea formed is filtered off and the filtrate concentrated in vacuo to an oil. A second crop of the thiourea is obtained via trituration of the crude isothiocyanate with hexane. 4-methylbenzhydrylisothiocyanate is obtained as an oil; IR (neat) 2080 cm^-1. The material is of sufficient purity to use in the next step.

B. N-(4-Methylbenzhydryl)thiourea:

A solution of 22 g (0.092 mole) of 4-methylbenzhydrylisothiocyanate in 250 ml of dry ether at 0°C is treated with anhydrous NH₃ for 3 hours while stirring. The mixture is stirred an additional 1.5 hours at 0 to 10°C during which time the crude product precipitates as a white solid. Filtration and washing with Et₂0 affords the product; mp. 167-168°C.

C. Methyl N-(4-Methylbenzhydryl)-carbamimidothioate Hydroiodide (and free base):

A suspension of 16.5 g (0.064 mole) of N-(4-methylbenz- hydrylthiourea in 75 ml of MeOH is treated with 9.08 g (0.064 mole) of methyl iodide and allowed to stir overnight at room temperature. The reaction mixture is concentrated in vacuo to yield 26.5 g (100%) of the crude product, which crystallizes. Recrystallization from t-BuOH affords pure methyl N-(4-methylbenzhydryl)carbamimido- thioate hydroiodide; mp. 145-147°C.
The free base of the product is obtained by treatment of the salt with ammonium hydroxide. Recrystallization of the free base from ether-hexane furnishes pure methyl N-(4-methylbenzhydryl)-carbamimidothioate; mp. 130-132°C.

D. N-(4-Methylbenzhydryl)-1-pyrrolidinecarboximidamide Hydroiodide:

A mixture of 13.3 g (0.0334 mole) of methyl N-(4-methyl- benzhydryl)carbamimidothioate hydroiodide and 5.0 g (0.07 mole) of pyrrolidine in 40 ml of t-BuOH is heated under reflux for 24 hrs. The crude product crystallizes out of the reaction mixture while refluxing. The mixture is allowed to cool overnight at room temperature and filtered to yield crude product; mp. 227-230°C. Recrystallization from MeOH/t-BuOH yields the pure N-(4-methyl- benzhydryl)-1-pyrrolidinecarboximidamide hydroiodide; mp. 229-230°C, as a white solid.

EXAMPLE XI

By repeating the amine-to-isothiocyanate procedure of Example VI-A or X-A, except that an equivalent amount of an appropriate benzhydrylamine is employed, the following benzhydrylisothiocyanates of Formula (V) are:

4,4'-dichlorobenzhydrylisothiocyanate;
4,4'-diethoxybenzhydrylisothiocyanate;
4-methoxybenzhydrylisothiocyanate;
4,4'-dimethoxybenzhydrylisothiocyanate;
4,4'-dibromobenzhydrylisothiocyanate;
3-bromobenzhydrylisothiocyanate; and
4,4'-dimethylbenzhydrylisothiocyanate.

EXAMPLE XII

The isothiocyanate-to-thiourea procedures of the applicable foregoing examples are followed using appropriate starting materials to yield the following benzhydrylthio- ureas of Formula (VI).

EXAMPLE XIII

A solution of an appropriate benzhydrylthiourea of Formula (VI) is S-methylated with an appropriate methylating agent (as indicated by the resultant HX salt below) to furnish the following methyl N-benzhydryl-N'-R₁-carbamimidothioates of Formula (II) in the form of an acid addition (HX) salt:

EXAMPLE XIV

By heating an appropriate methyl N_-benzhydryl-N'-R-carbamimidothioate salt of Formula (II) with an appropriate R₂R₃NH amine of Formula (III) in the indicated molar ratios at reflux temperature in either isopropanol or t-butanol according to the procedures previously described, the following benzhydryl guanidine derivatives of Formula (I) are obtained in the form of the indicated acid addition (HX) salt:

Claims

1. Benzhydryl guanidine derivatives having the formula I

wherein:

R₁ is a member selected from the group consisting of hydrogen and lower alkyl, preferably methyl and ethyl;

R₂ is a member selected from the group consisting of hydrogen and lower alkyl, preferably methyl and ethyl;

R₃ is a member selected from the group consisting of hydrogen, lower alkyl, preferably methyl and ethyl, and cycloalkyl, preferably cyclopentyl and cyclohexyl;

taken together represents a member selected from the group consisting of

and

X and Z are each a member selected from the group consisting of hydrogen, halo, lower alkyl, preferably methyl, and lower alkyloxy, preferably methoxy and ethoxy;

and acid addition salts thereof.

2. N-Benzhydryl-1-pyrrolidinecarboximidamide and acid addition salts thereof.

3. N-Benzhydryl-4-morpholinecarboximidamide and acid addition salts thereof.

4. N-Benzhydryl-N',N'-diethylguanidine and acid addition salts thereof.

5. N-Benzhydryl-1-piperidinecarboximidamide and acid addition salts thereof.

6. N-Benzhydryl-1-(4-methyl)piperazinecarboximidamide and acid addition salts thereof.

7. N-(4-Chlorobenzhydryl)-1-pyrrolidinecarboximidamide and acid addition salts thereof.

8. N-Benzhydryl-N'-cyclopentyl-N'-methylguanidine and acid addition salts thereof.

9. N-Benzylhydryl-4-phenyl-1-piperazinecarboximidamide and acid addition salts thereof.

10. N-Benzhydryl-N'-methyl-1-pyrrolidinecarboximidamide and acid addition salts thereof.

11. N-(4-methylbenzhydryl)-1-pyrrolidinecarboximidamide and acid addition salts thereof.

12. A pharmaceutical composition comprising a member selected from the group consisting of a benzhydryl guanidine derivate having the formula I

wherein:

R₁is a member selected from the group consisting of hydrogen and lower alkyl, preferably methyl and ethyl;

taken together represents a member selected from the group consisting of

and

and acid addition salts thereof in admixture with a pharmaceutical carrier.