EA202190809A1 - REPRODUCTION OF OIL FROM CRYOPRESERVED TUMOR SAMPLES - Google Patents
REPRODUCTION OF OIL FROM CRYOPRESERVED TUMOR SAMPLESInfo
- Publication number
- EA202190809A1 EA202190809A1 EA202190809A EA202190809A EA202190809A1 EA 202190809 A1 EA202190809 A1 EA 202190809A1 EA 202190809 A EA202190809 A EA 202190809A EA 202190809 A EA202190809 A EA 202190809A EA 202190809 A1 EA202190809 A1 EA 202190809A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- tumor tissue
- fragments
- freezing
- reproduction
- oil
- Prior art date
Links
Abstract
Раскрыты способы размножения опухоль-инфильтрирующих лимфоцитов (ОИЛ) из криоконсервированной опухолевой ткани и способы применения размноженных ОИЛ в лечении заболеваний человека, включая рак. Подготовка опухолевой ткани для замораживания включает фрагментирование опухолевой ткани и инкубацию фрагментов в среде для криоконсервирования. В одном из вариантов осуществления фрагменты можно инкубировать в среде для криоконсервирования при температуре от 2 до 8°C в течение периода времени от примерно 30 до примерно 80 мин. Замораживание фрагментов можно осуществлять путем сверхбыстрого замораживания с использованием паровой фазы жидкого азота, например, используя криотару для сухой перевозки. В некоторых вариантах осуществления раскрыты композиции криоконсервированных опухолевых тканей.Disclosed are methods of multiplication of tumor-infiltrating lymphocytes (TIL) from cryopreserved tumor tissue and methods of using multiplied TIL in the treatment of human diseases, including cancer. Preparation of tumor tissue for freezing includes fragmentation of tumor tissue and incubation of the fragments in a cryopreservation medium. In one embodiment, the fragments can be incubated in cryopreservation medium at 2 to 8 ° C for a period of about 30 to about 80 minutes. Freezing of fragments can be accomplished by ultra-fast freezing using the vapor phase of liquid nitrogen, for example, using a cryotar for dry transport. In some embodiments, cryopreserved tumor tissue compositions are disclosed.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962879881P | 2019-07-29 | 2019-07-29 | |
| PCT/US2019/052108 WO2020061429A1 (en) | 2018-09-20 | 2019-09-20 | Expansion of tils from cryopreserved tumor samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA202190809A1 true EA202190809A1 (en) | 2021-08-19 |
Family
ID=77515166
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA202190809A EA202190809A1 (en) | 2019-07-29 | 2019-09-20 | REPRODUCTION OF OIL FROM CRYOPRESERVED TUMOR SAMPLES |
Country Status (1)
| Country | Link |
|---|---|
| EA (1) | EA202190809A1 (en) |
-
2019
- 2019-09-20 EA EA202190809A patent/EA202190809A1/en unknown
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