EA202190454A1 - NEW CRISPR-ASSOCIATED PROTEIN AND ITS APPLICATIONS - Google Patents

NEW CRISPR-ASSOCIATED PROTEIN AND ITS APPLICATIONS

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Publication number
EA202190454A1
EA202190454A1 EA202190454A EA202190454A EA202190454A1 EA 202190454 A1 EA202190454 A1 EA 202190454A1 EA 202190454 A EA202190454 A EA 202190454A EA 202190454 A EA202190454 A EA 202190454A EA 202190454 A1 EA202190454 A1 EA 202190454A1
Authority
EA
Eurasian Patent Office
Prior art keywords
associated protein
crispr
applications
present
protein
Prior art date
Application number
EA202190454A
Other languages
Russian (ru)
Inventor
Сонхва Чое
Хан Сон Ким
Тон Ук Ким
Чончин Парк
Чиюн Юн
Original Assignee
Джи+ФЛАС ЛАЙФ САЙЕНСИЗ
СЕУЛ НЭШНЛ ЮНИВЕРСИТИ Ар энд ДиБи ФАУНДЕЙШН
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Джи+ФЛАС ЛАЙФ САЙЕНСИЗ, СЕУЛ НЭШНЛ ЮНИВЕРСИТИ Ар энд ДиБи ФАУНДЕЙШН filed Critical Джи+ФЛАС ЛАЙФ САЙЕНСИЗ
Publication of EA202190454A1 publication Critical patent/EA202190454A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Abstract

Настоящее изобретение относится к новому CRISPR-ассоциированному белку и к его применению. Белок, имеющий аминокислотную последовательность SEQ ID NO: 1 или SEQ ID NO: 3, в соответствии с настоящим изобретением обладает эндонуклеазной активностью, способной распознавать и расщеплять внутриклеточную нуклеотидную последовательность, связанную с направляющей РНК. Следовательно, новый CRISPR-ассоциированный белок по настоящему изобретению можно использовать в качестве другой нуклеазы для редактирования генома в системе CRISPR-Cas.The present invention relates to a novel CRISPR-associated protein and its use. The protein having the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 3 according to the present invention has an endonuclease activity capable of recognizing and cleaving an intracellular nucleotide sequence associated with a guide RNA. Therefore, the novel CRISPR-associated protein of the present invention can be used as another nuclease for genome editing in the CRISPR-Cas system.

EA202190454A 2018-08-09 2019-08-09 NEW CRISPR-ASSOCIATED PROTEIN AND ITS APPLICATIONS EA202190454A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20180093336 2018-08-09
PCT/KR2019/010110 WO2020032711A1 (en) 2018-08-09 2019-08-09 Novel crispr-associated protein and use thereof

Publications (1)

Publication Number Publication Date
EA202190454A1 true EA202190454A1 (en) 2021-04-22

Family

ID=69415629

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202190454A EA202190454A1 (en) 2018-08-09 2019-08-09 NEW CRISPR-ASSOCIATED PROTEIN AND ITS APPLICATIONS

Country Status (15)

Country Link
US (1) US20210292722A1 (en)
EP (1) EP3835418A4 (en)
JP (1) JP2021532819A (en)
KR (2) KR102096592B1 (en)
CN (1) CN112567031A (en)
AU (1) AU2019319377A1 (en)
BR (1) BR112021002476A2 (en)
CA (1) CA3109105A1 (en)
EA (1) EA202190454A1 (en)
IL (1) IL280631A (en)
MX (1) MX2021001578A (en)
PH (1) PH12021550256A1 (en)
SG (1) SG11202101227TA (en)
WO (1) WO2020032711A1 (en)
ZA (1) ZA202101250B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
WO2020030984A2 (en) 2018-08-09 2020-02-13 G+Flas Life Sciences Compositions and methods for genome engineering with cas12a proteins
WO2022065867A1 (en) * 2020-09-22 2022-03-31 (주)지플러스생명과학 Modified cas12a protein and use thereof
KR102497690B1 (en) * 2020-09-22 2023-02-10 (주)지플러스생명과학 Novel CRISPR Associated Protein and Use thereof
WO2024062138A1 (en) 2022-09-23 2024-03-28 Mnemo Therapeutics Immune cells comprising a modified suv39h1 gene

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
EP4273782A3 (en) * 2015-07-25 2023-12-27 Frost, Habib A system, device and a method for providing a therapy or a cure for cancer and other pathological states
EP3374494A4 (en) * 2015-11-11 2019-05-01 Coda Biotherapeutics, Inc. Crispr compositions and methods of using the same for gene therapy
SG11201803593QA (en) * 2015-12-04 2018-06-28 Caribou Biosciences Inc Engineered nucleic-acid targeting nucleic acids
WO2017099494A1 (en) * 2015-12-08 2017-06-15 기초과학연구원 Genome editing composition comprising cpf1, and use thereof
US20190264186A1 (en) * 2016-01-22 2019-08-29 The Broad Institute Inc. Crystal structure of crispr cpf1
US9896696B2 (en) * 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
EP3445853A1 (en) * 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
WO2018013990A1 (en) * 2016-07-15 2018-01-18 Zymergen Inc. Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase
WO2018071672A1 (en) * 2016-10-12 2018-04-19 The Regents Of The University Of Colorado Novel engineered and chimeric nucleases
KR20180018466A (en) * 2017-11-10 2018-02-21 주식회사 툴젠 Composition for modulating activity of immune regulatory gene in immune cell and Use thereof

Also Published As

Publication number Publication date
AU2019319377A1 (en) 2021-03-11
US20210292722A1 (en) 2021-09-23
JP2021532819A (en) 2021-12-02
PH12021550256A1 (en) 2021-11-03
ZA202101250B (en) 2022-09-28
CN112567031A (en) 2021-03-26
KR20200018345A (en) 2020-02-19
KR102096604B1 (en) 2020-04-02
EP3835418A4 (en) 2022-05-04
EP3835418A1 (en) 2021-06-16
SG11202101227TA (en) 2021-03-30
MX2021001578A (en) 2021-06-15
KR20200018364A (en) 2020-02-19
IL280631A (en) 2021-03-25
CA3109105A1 (en) 2020-02-13
BR112021002476A2 (en) 2021-07-27
KR102096592B1 (en) 2020-04-02
WO2020032711A1 (en) 2020-02-13

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