EA202092286A1 - GENERAL AFUCOSYLATED ANTIBODY GLYCOFORM OBTAINED IN CELL CULTURE - Google Patents

GENERAL AFUCOSYLATED ANTIBODY GLYCOFORM OBTAINED IN CELL CULTURE

Info

Publication number
EA202092286A1
EA202092286A1 EA202092286A EA202092286A EA202092286A1 EA 202092286 A1 EA202092286 A1 EA 202092286A1 EA 202092286 A EA202092286 A EA 202092286A EA 202092286 A EA202092286 A EA 202092286A EA 202092286 A1 EA202092286 A1 EA 202092286A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cell culture
general
glycoforms
taf
afucosylated antibody
Prior art date
Application number
EA202092286A
Other languages
Russian (ru)
Inventor
Принс Бхебе
Мэттью Джерумс
Айрин Лю
Курт Кунас
Original Assignee
Эмджен Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Эмджен Инк. filed Critical Эмджен Инк.
Publication of EA202092286A1 publication Critical patent/EA202092286A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
    • C07K2317/732—Antibody-dependent cellular cytotoxicity [ADCC]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

В изобретение предусмотрены способы получения композиции на основе антитела, содержащей общие афукозилированные (TAF) гликоформы на требуемом, или предварительно определенном, или предварительно выбранном уровне. В иллюстративных вариантах осуществления способ включает поддержание компетентных в отношении гликозилирования клеток в среде для культивирования клеток, содержащей фукозу и/или глюкозу в определенной концентрации, описанной в изобретение, зависящей от требуемого уровня TAF-гликоформ. Также в изобретение предусмотрены соответствующие композиции, содержащие гликозилированные белки и их TAF-гликоформы. Также предусмотрены среды для культивирования клеток.The invention provides methods for preparing an antibody composition comprising total aphucosylated (TAF) glycoforms at a desired or predetermined or preselected level. In illustrative embodiments, the method comprises maintaining glycosylation-competent cells in a cell culture medium containing fucose and / or glucose at a specific concentration as described herein, depending on the desired level of TAF glycoforms. Also provided by the invention are corresponding compositions comprising glycosylated proteins and their TAF glycoforms. Cell culture media are also provided.

EA202092286A 2018-03-26 2019-03-26 GENERAL AFUCOSYLATED ANTIBODY GLYCOFORM OBTAINED IN CELL CULTURE EA202092286A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862648308P 2018-03-26 2018-03-26
PCT/US2019/024154 WO2019191150A1 (en) 2018-03-26 2019-03-26 Total afucosylated glycoforms of antibodies produced in cell culture

Publications (1)

Publication Number Publication Date
EA202092286A1 true EA202092286A1 (en) 2021-03-18

Family

ID=66102281

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202092286A EA202092286A1 (en) 2018-03-26 2019-03-26 GENERAL AFUCOSYLATED ANTIBODY GLYCOFORM OBTAINED IN CELL CULTURE

Country Status (15)

Country Link
US (1) US20210079065A1 (en)
EP (1) EP3775251A1 (en)
JP (2) JP2021519068A (en)
KR (1) KR20200135781A (en)
CN (1) CN111954719A (en)
AU (1) AU2019243848A1 (en)
BR (1) BR112020019559A2 (en)
CA (1) CA3093853A1 (en)
CL (1) CL2020002450A1 (en)
EA (1) EA202092286A1 (en)
IL (2) IL313983A (en)
MA (1) MA52186A (en)
MX (1) MX2020009907A (en)
SG (1) SG11202009216YA (en)
WO (1) WO2019191150A1 (en)

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EP4229080A1 (en) 2020-10-15 2023-08-23 Amgen Inc. Relative unpaired glycans in antibody production methods
AR126089A1 (en) 2021-06-07 2023-09-13 Amgen Inc USE OF FUCOSIDASE TO CONTROL THE LEVEL OF AFFUCOSYLATION OF GLUCOSYLATED PROTEINS
CN113960232B (en) * 2021-10-28 2024-02-20 苏州大学 Saliva-specific-fucosylation-based structural glycoprofile, and detection method and application thereof

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SG11202009216YA (en) 2020-10-29
JP2021519068A (en) 2021-08-10
IL276910B1 (en) 2024-08-01
US20210079065A1 (en) 2021-03-18
AU2019243848A1 (en) 2020-09-17
BR112020019559A2 (en) 2021-01-12
IL276910A (en) 2020-10-29
WO2019191150A1 (en) 2019-10-03
MX2020009907A (en) 2020-10-14
CL2020002450A1 (en) 2020-12-28
MA52186A (en) 2021-02-17
EP3775251A1 (en) 2021-02-17
KR20200135781A (en) 2020-12-03
JP2024019717A (en) 2024-02-09
CN111954719A (en) 2020-11-17
CA3093853A1 (en) 2019-10-03
IL313983A (en) 2024-08-01

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