EA201800542A1 - MODIFIED RADIOLIGAND METHOD FOR QUANTITATIVE ASSESSMENT OF BETA-ADRENOREPTEPTOR BINDING ACTIVITY ON SURFACE OF HUMAN T-Lymphocytes - Google Patents

MODIFIED RADIOLIGAND METHOD FOR QUANTITATIVE ASSESSMENT OF BETA-ADRENOREPTEPTOR BINDING ACTIVITY ON SURFACE OF HUMAN T-Lymphocytes

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Publication number
EA201800542A1
EA201800542A1 EA201800542A EA201800542A EA201800542A1 EA 201800542 A1 EA201800542 A1 EA 201800542A1 EA 201800542 A EA201800542 A EA 201800542A EA 201800542 A EA201800542 A EA 201800542A EA 201800542 A1 EA201800542 A1 EA 201800542A1
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EA
Eurasian Patent Office
Prior art keywords
lymphocytes
binding activity
human
modified
adrenorepteptor
Prior art date
Application number
EA201800542A
Other languages
Russian (ru)
Other versions
EA036081B1 (en
Inventor
Кирилл Алексеевич Зыков
Екатерина Владимировна Смолякова
Юрий Самойлович Скоблов
Наталья Александровна Скоблова
Валерий Павлович Масенко
Лали Гурамовна Амбатьелло
Анна Валерьевна Рвачёва
Ирина Евгеньевна Чазова
Original Assignee
Федеральное Государственное Бюджетное Учреждение "Научно-Исследовательский Институт Пульмонологии" Федерального Медико-Биологического Агентства (Фгбу "Нии Пульмонологии" Фмба России
Государственное Бюджетное Образовательное Учреждение Высшего Профессионального Образования "Московский Государственный Медико-Стоматологический Университет Имени А.И. Евдокимова" Министерства Здравоохранения Российской Федерации (Гбоу Впо Мгмсу Им. А.И. Евдокимова Минздрава России)
Федеральное Государственное Бюджетное Учреждение "Национальный Медицинский Исследовательский Центр Кардиологии" Министерства Здравоохранения Российской Федерации (Фгбу "Нмицк" России)
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Application filed by Федеральное Государственное Бюджетное Учреждение "Научно-Исследовательский Институт Пульмонологии" Федерального Медико-Биологического Агентства (Фгбу "Нии Пульмонологии" Фмба России, Государственное Бюджетное Образовательное Учреждение Высшего Профессионального Образования "Московский Государственный Медико-Стоматологический Университет Имени А.И. Евдокимова" Министерства Здравоохранения Российской Федерации (Гбоу Впо Мгмсу Им. А.И. Евдокимова Минздрава России), Федеральное Государственное Бюджетное Учреждение "Национальный Медицинский Исследовательский Центр Кардиологии" Министерства Здравоохранения Российской Федерации (Фгбу "Нмицк" России) filed Critical Федеральное Государственное Бюджетное Учреждение "Научно-Исследовательский Институт Пульмонологии" Федерального Медико-Биологического Агентства (Фгбу "Нии Пульмонологии" Фмба России
Priority to EA201800542A priority Critical patent/EA036081B1/en
Publication of EA201800542A1 publication Critical patent/EA201800542A1/en
Publication of EA036081B1 publication Critical patent/EA036081B1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Изобретение относится к области медицины и обеспечивает модифицированный радиолигандный способ количественной оценки активности связывания -адренорецепторов (-AP) на поверхности T-лимфоцитов человека, позволяющий достоверно определять специфическое связывание с 1- и 2-адренорецепторами при их малых концентрациях, на основе которого могут быть выполнены анализы для клинических исследований. Способ включает стадию предынкубации среды, содержащей T-лимфоциты, с лигандом, специфическим по отношению к 1- и 2-AP, с последующим взаимодействием с [I]цианопиндололом. Техническими результатами являются существенное повышение чувствительности анализа в отношении активности связывания 1-AP, а также возможность выявления характерных паттернов изменения характеристик -AP звена под влиянием предынкубации со специфическими лигандами.The invention relates to the field of medicine and provides a modified radioligand method for quantitatively assessing the binding activity of β-adrenergic receptors (α-AP) on the surface of human T-lymphocytes, which allows to reliably determine specific binding with 1- and 2-adrenergic receptors at their low concentrations, on the basis of which analyzes for clinical trials. The method includes the stage of pre-incubation of a medium containing T-lymphocytes with a ligand specific for 1- and 2-AP, followed by interaction with [I] cyanopindolol. The technical results are a significant increase in the sensitivity of the assay in relation to the binding activity of 1-AP, as well as the ability to identify characteristic patterns of changes in the characteristics of the -AP link under the influence of pre-incubation with specific ligands.

EA201800542A 2018-11-01 2018-11-01 Modified radioligand method for quantitative assessment of beta-adrenoreceptor binding activity on human t-lymphocyte surface EA036081B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EA201800542A EA036081B1 (en) 2018-11-01 2018-11-01 Modified radioligand method for quantitative assessment of beta-adrenoreceptor binding activity on human t-lymphocyte surface

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EA201800542A EA036081B1 (en) 2018-11-01 2018-11-01 Modified radioligand method for quantitative assessment of beta-adrenoreceptor binding activity on human t-lymphocyte surface

Publications (2)

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EA201800542A1 true EA201800542A1 (en) 2020-05-29
EA036081B1 EA036081B1 (en) 2020-09-23

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Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA026837B1 (en) * 2015-06-22 2017-05-31 Федеральное Государственное Бюджетное Учреждение Российский Кардиологический Научно-Производственный Комплекс Министерства Здравоохранения Российской Федерации (Фгбу "Ркнпк" Минздрава России) RADIOLIGAND METHOD FOR QUANTITATION OF RECEPTOR ACTIVITY OF β-ADRENORECEPTORS ON THE SURGACE OF HUMAN T-LYMPHOCYTES

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EA036081B1 (en) 2020-09-23

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG TJ TM RU