EA201792329A1 - METHOD OF MONOMERIZATION OF RECOMBINANT ANTIBODY MOLECULES - Google Patents

METHOD OF MONOMERIZATION OF RECOMBINANT ANTIBODY MOLECULES

Info

Publication number
EA201792329A1
EA201792329A1 EA201792329A EA201792329A EA201792329A1 EA 201792329 A1 EA201792329 A1 EA 201792329A1 EA 201792329 A EA201792329 A EA 201792329A EA 201792329 A EA201792329 A EA 201792329A EA 201792329 A1 EA201792329 A1 EA 201792329A1
Authority
EA
Eurasian Patent Office
Prior art keywords
antibody molecules
monomerization
recombinant antibody
composition
reducing agent
Prior art date
Application number
EA201792329A
Other languages
Russian (ru)
Inventor
Сэм Филип Хейвуд
Гэвин Бэрри Уайлд
Разван Ханиф
Паж Кристофер Джон Ле
Original Assignee
Юсб Биофарма Спрл
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Юсб Биофарма Спрл filed Critical Юсб Биофарма Спрл
Publication of EA201792329A1 publication Critical patent/EA201792329A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39591—Stabilisation, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
    • C07C323/10—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton
    • C07C323/11—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/12—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and singly-bound oxygen atoms bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
    • C07C323/23—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton
    • C07C323/24—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/25—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/12—Cyclic peptides with only normal peptide bonds in the ring
    • C07K5/123—Tripeptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

В настоящем изобретении предлагается способ увеличения процента мономера в композиции молекул рекомбинантно экспрессированного антитела, отличающийся тем, что молекула антитела содержит по меньшей мере один Fv со специфичностью к интересующему антигену, содержащий один VH и один VL, где указанные VH и VL связаны прямо или косвенно через один или несколько линкеров и стабилизируются дисульфидной связью между ними, причем указанный способ включает: a) стадию конверсии обработки композиции денатурирующим агентом, выбранным из мочевины и/или гидрохлорида гуанидина; b) где стадию a) проводят в присутствии восстанавливающего агента или после обработки восстанавливающим агентом.The present invention proposes a method of increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules, characterized in that the antibody molecule contains at least one Fv with specificity for the antigen of interest, containing one VH and one VL, where said VH and VL are linked directly or indirectly through one or more linkers and are stabilized by a disulfide bond between them, and this method includes: a) the conversion stage of processing the composition with a denaturing agent selected from urea and / or g drohlorida guanidine; b) where stage a) is carried out in the presence of a reducing agent or after treatment with a reducing agent.

EA201792329A 2015-04-22 2016-04-22 METHOD OF MONOMERIZATION OF RECOMBINANT ANTIBODY MOLECULES EA201792329A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1506870.3A GB201506870D0 (en) 2015-04-22 2015-04-22 Method
PCT/EP2016/059051 WO2016170138A1 (en) 2015-04-22 2016-04-22 Method of monomerisation of recombinant antibody molecules

Publications (1)

Publication Number Publication Date
EA201792329A1 true EA201792329A1 (en) 2018-02-28

Family

ID=53299019

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201792329A EA201792329A1 (en) 2015-04-22 2016-04-22 METHOD OF MONOMERIZATION OF RECOMBINANT ANTIBODY MOLECULES

Country Status (17)

Country Link
US (2) US10828366B2 (en)
EP (1) EP3286204B1 (en)
JP (1) JP6898251B2 (en)
KR (1) KR20170138552A (en)
CN (1) CN108064230B (en)
AU (1) AU2016251620A1 (en)
BR (1) BR112017021812A2 (en)
CA (1) CA2981644C (en)
CL (1) CL2017002676A1 (en)
CO (1) CO2017010385A2 (en)
EA (1) EA201792329A1 (en)
ES (1) ES2846732T3 (en)
GB (1) GB201506870D0 (en)
IL (1) IL254765A0 (en)
MX (1) MX2017013376A (en)
SG (1) SG11201708151TA (en)
WO (1) WO2016170138A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201223276D0 (en) * 2012-12-21 2013-02-06 Ucb Pharma Sa Antibodies and methods of producing same
JP6807326B2 (en) 2014-12-22 2021-01-06 ユーシービー バイオファルマ エスアールエル Protein production method
GB201506869D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method
GB201506870D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method
GB201506868D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method for protein purification
MX2021003976A (en) * 2018-10-11 2021-05-27 Amgen Inc Downstream processing of bispecific antibody constructs.
MX2023006650A (en) * 2020-12-07 2023-06-21 UCB Biopharma SRL Multi-specific antibodies and antibody combinations.

Family Cites Families (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4741900A (en) 1982-11-16 1988-05-03 Cytogen Corporation Antibody-metal ion complexes
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
DK336987D0 (en) 1987-07-01 1987-07-01 Novo Industri As immobilization
US5453363A (en) 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
GB8719042D0 (en) 1987-08-12 1987-09-16 Parker D Conjugate compounds
GB8720833D0 (en) 1987-09-04 1987-10-14 Celltech Ltd Recombinant dna product
ATE151110T1 (en) 1988-09-02 1997-04-15 Protein Eng Corp PRODUCTION AND SELECTION OF RECOMBINANT PROTEINS WITH DIFFERENT BINDING SITES
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US5750373A (en) 1990-12-03 1998-05-12 Genentech, Inc. Enrichment method for variant proteins having altered binding properties, M13 phagemids, and growth hormone variants
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
GB8907617D0 (en) 1989-04-05 1989-05-17 Celltech Ltd Drug delivery system
GB8928874D0 (en) 1989-12-21 1990-02-28 Celltech Ltd Humanised antibodies
WO1991010737A1 (en) 1990-01-11 1991-07-25 Molecular Affinities Corporation Production of antibodies using gene libraries
US5780225A (en) 1990-01-12 1998-07-14 Stratagene Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
AU633698B2 (en) 1990-01-12 1993-02-04 Amgen Fremont Inc. Generation of xenogeneic antibodies
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
EP0542810A1 (en) 1990-08-02 1993-05-26 B.R. Centre Limited Methods for the production of proteins with a desired function
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
WO1992003918A1 (en) 1990-08-29 1992-03-19 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5770429A (en) 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5698426A (en) 1990-09-28 1997-12-16 Ixsys, Incorporated Surface expression libraries of heteromeric receptors
JP3672306B2 (en) 1991-04-10 2005-07-20 ザ スクリップス リサーチ インスティテュート Heterodimeric receptor library using phagemids
GB9112536D0 (en) 1991-06-11 1991-07-31 Celltech Ltd Chemical compounds
GB9120467D0 (en) 1991-09-26 1991-11-06 Celltech Ltd Anti-hmfg antibodies and process for their production
ATE275198T1 (en) 1991-12-02 2004-09-15 Medical Res Council PRODUCTION OF ANTIBODIES ON PHAGE SURFACES BASED ON ANTIBODIES SEGMENT LIBRARIES.
AU675929B2 (en) * 1992-02-06 1997-02-27 Curis, Inc. Biosynthetic binding protein for cancer marker
US5733743A (en) 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
WO1995015982A2 (en) 1993-12-08 1995-06-15 Genzyme Corporation Process for generating specific antibodies
WO1995020401A1 (en) 1994-01-31 1995-08-03 Trustees Of Boston University Polyclonal antibody libraries
FR2716640B1 (en) 1994-02-28 1996-05-03 Procedes Machines Speciales Device for centering and blocking a workpiece with a view to running it in using an expansion lapper.
US5516637A (en) 1994-06-10 1996-05-14 Dade International Inc. Method involving display of protein binding pairs on the surface of bacterial pili and bacteriophage
JP2978435B2 (en) 1996-01-24 1999-11-15 チッソ株式会社 Method for producing acryloxypropyl silane
US5980898A (en) 1996-11-14 1999-11-09 The United States Of America As Represented By The U.S. Army Medical Research & Material Command Adjuvant for transcutaneous immunization
GB9625640D0 (en) 1996-12-10 1997-01-29 Celltech Therapeutics Ltd Biological products
ES2366100T3 (en) * 1999-02-22 2011-10-17 Georgetown University DIRECTED IMMUNOLIPOSOMES THROUGH AN ANTIBODY FRAGMENT FOR THE SYSTEMS ADMINISTRATION OF GENES.
US6908963B2 (en) 2001-10-09 2005-06-21 Nektar Therapeutics Al, Corporation Thioester polymer derivatives and method of modifying the N-terminus of a polypeptide therewith
BR122019027966B8 (en) 2002-05-02 2021-07-27 Wyeth Corp monomeric calicheamicin derivative/anti-cd22 antibody conjugate and their use
AU2003223936A1 (en) * 2002-05-17 2003-12-02 Kobenhavns Universitet Method for purifying denatured proteins having a desired disulfide bond configuration
US7993864B2 (en) 2002-12-03 2011-08-09 Ucb Pharma S.A. Assay for identifying antibody producing cells
GB0312481D0 (en) 2003-05-30 2003-07-09 Celltech R&D Ltd Antibodies
US7989594B2 (en) 2003-07-01 2011-08-02 Celltech R & D Limited Modified antibody fab fragments
GB0315457D0 (en) 2003-07-01 2003-08-06 Celltech R&D Ltd Biological products
GB0315450D0 (en) * 2003-07-01 2003-08-06 Celltech R&D Ltd Biological products
KR101200133B1 (en) 2004-06-01 2012-11-13 제넨테크, 인크. Antibody drug conjugates and methods
GB0412181D0 (en) 2004-06-01 2004-06-30 Celltech R&D Ltd Biological products
US20060051347A1 (en) 2004-09-09 2006-03-09 Winter Charles M Process for concentration of antibodies and therapeutic products thereof
AU2005299716B2 (en) * 2004-10-22 2012-09-06 Amgen Inc. Methods for refolding of recombinant antibodies
US20070021591A1 (en) 2005-07-25 2007-01-25 Trubion Pharmaceuticals, Inc. Compositions and methods for protein deaggregation
EP1929073A4 (en) 2005-09-27 2010-03-10 Amunix Inc Proteinaceous pharmaceuticals and uses thereof
GB0619291D0 (en) 2006-09-29 2006-11-08 Ucb Sa Altered antibodies
EP2535349A1 (en) 2007-09-26 2012-12-19 UCB Pharma S.A. Dual specificity antibody fusions
US20110144311A1 (en) 2008-08-14 2011-06-16 Rebecca Chmielowski Methods for purifying antibodies using protein a affinity chromatography
US10407513B2 (en) 2008-09-26 2019-09-10 Ucb Biopharma Sprl Biological products
CN102224239A (en) * 2008-09-26 2011-10-19 先灵公司 High titer antibody production
MX2011013417A (en) 2009-06-25 2012-03-29 Amgen Inc Capture purification processes for proteins expressed in a non-mammalian system.
EP2475682B1 (en) 2009-09-10 2018-01-31 UCB Biopharma SPRL Multivalent antibodies
GB201005063D0 (en) 2010-03-25 2010-05-12 Ucb Pharma Sa Biological products
GB0920127D0 (en) * 2009-11-17 2009-12-30 Ucb Pharma Sa Antibodies
GB201000467D0 (en) 2010-01-12 2010-02-24 Ucb Pharma Sa Antibodies
CN102892780B (en) * 2010-03-17 2015-07-29 通益制药有限公司 Obtain the method for bioactive recombinant human g-csf
TR201903279T4 (en) 2010-03-25 2019-03-21 Ucb Biopharma Sprl Disulfide stabilized DVD-IG molecules.
GB201005064D0 (en) 2010-03-25 2010-05-12 Ucb Pharma Sa Biological products
WO2012040216A1 (en) * 2010-09-20 2012-03-29 Bio-Rad Laboratories, Inc. Dissociation of product-complexed contaminants in chromatography
US8759617B2 (en) * 2010-09-21 2014-06-24 National Institute Of Agrobiological Sciences Method for extraction and purification of recombinant proteins from transgenic plants
JP2014527072A (en) * 2011-09-09 2014-10-09 バイオメド リアルティー， エル．ピー． Methods and compositions for controlling the assembly of viral proteins
SG11201401649VA (en) 2011-11-11 2014-07-30 Ucb Pharma Sa Albumin binding antibodies and binding fragments thereof
UA112203C2 (en) 2011-11-11 2016-08-10 Юсб Фарма С.А. Fusion protein of a biospecific antibody that binds to human OX40 and serum human albumin
US20160060291A1 (en) 2012-03-30 2016-03-03 Biogenomics Limited Process for renaturation of polypeptides
GB201208370D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Antibodies
LT3447069T (en) 2012-11-21 2020-12-10 Janssen Biotech, Inc. Bispecific egfr/c-met antibodies
WO2015134785A1 (en) 2014-03-07 2015-09-11 Bionano Genomics, Inc. Processing of polynucleotides
GB201411320D0 (en) 2014-06-25 2014-08-06 Ucb Biopharma Sprl Antibody construct
GB201506868D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method for protein purification
GB201506870D0 (en) * 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method
GB201506869D0 (en) 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method
EP3485280A1 (en) 2016-08-12 2019-05-22 Lonza Ltd Proteomic analysis of host cell proteins

Also Published As

Publication number Publication date
KR20170138552A (en) 2017-12-15
JP6898251B2 (en) 2021-07-07
AU2016251620A1 (en) 2017-10-26
EP3286204B1 (en) 2020-11-18
CL2017002676A1 (en) 2018-03-23
US11786593B2 (en) 2023-10-17
SG11201708151TA (en) 2017-11-29
CA2981644A1 (en) 2016-10-27
US20180117153A1 (en) 2018-05-03
JP2018515449A (en) 2018-06-14
EP3286204A1 (en) 2018-02-28
CN108064230B (en) 2021-04-06
GB201506870D0 (en) 2015-06-03
WO2016170138A1 (en) 2016-10-27
CO2017010385A2 (en) 2018-03-20
US20210069328A1 (en) 2021-03-11
IL254765A0 (en) 2017-12-31
US10828366B2 (en) 2020-11-10
CN108064230A (en) 2018-05-22
CA2981644C (en) 2023-07-04
MX2017013376A (en) 2017-12-07
BR112017021812A2 (en) 2018-07-10
ES2846732T3 (en) 2021-07-29

Similar Documents

Publication Publication Date Title
EA201792329A1 (en) METHOD OF MONOMERIZATION OF RECOMBINANT ANTIBODY MOLECULES
EA201891983A1 (en) COMBINED THERAPY WITH ANTIBODIES TO CD73
PE20181805A1 (en) COMPOSITIONS AND ANTI-TIM-3 ANTIBODIES
PE20170665A1 (en) HUMANIZED ANTI-TAU ANTIBODIES
EA201491571A1 (en) MODIFIED BISPECIFIC IgG4 ANTIBODIES WITH ASYMMETRIC SEQUENCE
EA201792325A1 (en) METHOD OF INCREASING THE INTEREST CONTENT OF A MONOMARIAN ANTIBODY IN FAB-DSFV MULTIMERIC PARTICLES
AR104280A1 (en) COMBINATION THERAPY WITH COAGULATION FACTORS AND MULTI-SPECIFIC ANTIBODIES
EA201792221A1 (en) ANTIBODIES AGAINST SORTILINE AND METHODS OF THEIR APPLICATION
BR112017014308A2 (en) bispecific antibodies to plasma kallikrein and factor xii
EA201591153A1 (en) ANTIBODIES CONNECTING TL1A AND THEIR APPLICATION
PH12019502298A1 (en) Monoclonal antibody to pd-l1
EA202091747A1 (en) ANTIBODY B7-H4 COMPOSITIONS
EA201891990A1 (en) SLIP PROTEINS BASED ON RECOMBINANT FOLLYSTATIN AND FC-FRAGMENT AND THEIR APPLICATION IN THE TREATMENT OF MUSCULAR DYSTROPHY DYUSHEN
EA202092420A1 (en) ANTIBODY AGAINST PD-L1 AND ITS APPLICATION
UA120047C2 (en) Insulin-like growth factor 1 receptor -specific antibodies and uses thereof
EA201790816A1 (en) ANTIBODIES THAT ARE ASSOCIATED WITH CCR6 AND APPLICATION OPTIONS FOR THE INDICATED ANTIBODIES
CL2016001028A1 (en) High affinity and stable aggregation antibodies based on variable domains of vl and derivatives of vhh.
AR118763A1 (en) MONOCLONAL ANTIBODY SPECIFICALLY JOINING GITR
EA201991464A1 (en) ANTIBODY TO α-SINUCLEIN AND ITS APPLICATION
EA201990895A1 (en) ANTIBODIES TO O1 AND OPTIONS OF THEIR APPLICATION
MX2022014852A (en) METHODS FOR TREATING OR PREVENTING SARS-CoV-2 INFECTIONS AND COVID-19 WITH ANTI-SARS-CoV-2 SPIKE GLYCOPROTEIN ANTIBODIES.
PH12020551373A1 (en) Anti-phf-tau antibodies and uses thereof
EA202191666A1 (en) ANTIBODIES TO IL-27 AND THEIR USE
EA201891529A1 (en) COMPOSITIONS AND METHODS OF DETECTION AND TREATMENT OF OVARIAN CANCER
EA201691414A1 (en) ANTIBODIES TO MATRIX METALLOPROTEINASE 9 AND METHODS OF THEIR APPLICATION