EA201600076A1 - METHOD FOR ESTIMATING THE STATE OF AN ORGANISM BY SAMPLE OF BIOLOGICAL LIQUID, OBTAINED NONINVASIALLY - Google Patents

METHOD FOR ESTIMATING THE STATE OF AN ORGANISM BY SAMPLE OF BIOLOGICAL LIQUID, OBTAINED NONINVASIALLY

Info

Publication number
EA201600076A1
EA201600076A1 EA201600076A EA201600076A EA201600076A1 EA 201600076 A1 EA201600076 A1 EA 201600076A1 EA 201600076 A EA201600076 A EA 201600076A EA 201600076 A EA201600076 A EA 201600076A EA 201600076 A1 EA201600076 A1 EA 201600076A1
Authority
EA
Eurasian Patent Office
Prior art keywords
group
patients
biomarkers
pathological condition
test
Prior art date
Application number
EA201600076A
Other languages
Russian (ru)
Inventor
Людмила Владимировна БЕЛЬСКАЯ
Андрей Владимирович ТИТОВ
Елена Александровна САРФ
Виктор Константинович КОСЕНОК
Original Assignee
Андрей Владимирович ТИТОВ
Людмила Владимировна БЕЛЬСКАЯ
КОВАЛЕНКО, Дмитрий Анатольевич
ХАРИТОНАШВИЛИ, Ираклий Теймуразович
ЗАЙЧЕНКО, Николай Михайлович
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Андрей Владимирович ТИТОВ, Людмила Владимировна БЕЛЬСКАЯ, КОВАЛЕНКО, Дмитрий Анатольевич, ХАРИТОНАШВИЛИ, Ираклий Теймуразович, ЗАЙЧЕНКО, Николай Михайлович filed Critical Андрей Владимирович ТИТОВ
Priority to EA201600076A priority Critical patent/EA201600076A1/en
Priority to PCT/RU2016/000297 priority patent/WO2017116277A1/en
Publication of EA201600076A1 publication Critical patent/EA201600076A1/en

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/20—Supervised data analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B45/00—ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks

Abstract

Изобретение относится к методам определения патологических состояний человека с помощью биомаркеров, определяемых в слюне одним из ряда технологических подходов, в том числе, но не ограничиваясь, биохимическими методами, иммуноферментным анализом, методами капиллярного и гелевого электрофореза и т.д., а также технологии анализа многомерных данных в диагностических целях. Требуемый технический результат, заключающийся в упрощении и расширении области применения, достигается способом, согласно которому анализируют образцы биологической жидкости, в качестве которой используют цельную слюну, полученную от пациентов, составляют тестовый набор биомаркеров, содержащихся в образцах, полученных от пациентов тестовой группы, которая включает контрольную группу, состоящую из пациентов без патологии здоровья, а также исследуемую группу, которая включает пациентов с диагностируемым патологическим состоянием, при этом включение биомаркера в тестовый набор осуществляется по результатам статистического анализа его значений, определенных у пациентов тестовой группы, и определения критерия Вилкоксона между контрольной группой и исследуемой группой, а факт наличия или отсутствия патологического состояния определяют путем дискриминантного анализа значений биомаркеров тестового набора, определенных в цельной слюне пациента с диагностируемым патологическим состоянием и тестовой группы, причем при попадании результата дискриминантного анализа значений биомаркеров тестового набора пациента с диагностируемым патологическим состоянием в результаты контрольной группы делается заключение об отсутствии патологии, а при попадании в исследуемую группу - делается заключение о наличии патологии.The invention relates to methods for determining human pathological conditions using biomarkers determined in saliva by one of a number of technological approaches, including, but not limited to, biochemical methods, enzyme immunoassay, capillary and gel electrophoresis, etc., as well as analysis technology multidimensional data for diagnostic purposes. The required technical result, which consists in simplifying and expanding the scope, is achieved by the method according to which biological fluid samples are analyzed, which use whole saliva obtained from patients, are a test set of biomarkers contained in samples obtained from patients of the test group, which includes control group consisting of patients without health pathology, as well as the study group, which includes patients with a diagnosed pathological condition, p And this is the inclusion of the biomarker in the test set based on the results of a statistical analysis of its values determined in patients of the test group and the determination of the Wilcoxon criterion between the control group and the study group, and the fact of the presence or absence of a pathological condition is determined by discriminant analysis of the biomarkers of the test set defined whole saliva of a patient with a diagnosed pathological condition and the test group, and when the result of discriminant analysis biomarkers of the test set of a patient with a diagnosed pathological condition in the results of the control group, a conclusion is made about the absence of pathology, and if they fall into the studied group, a conclusion is made about the presence of pathology.

EA201600076A 2015-12-30 2015-12-30 METHOD FOR ESTIMATING THE STATE OF AN ORGANISM BY SAMPLE OF BIOLOGICAL LIQUID, OBTAINED NONINVASIALLY EA201600076A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EA201600076A EA201600076A1 (en) 2015-12-30 2015-12-30 METHOD FOR ESTIMATING THE STATE OF AN ORGANISM BY SAMPLE OF BIOLOGICAL LIQUID, OBTAINED NONINVASIALLY
PCT/RU2016/000297 WO2017116277A1 (en) 2015-12-30 2016-05-17 Method of evaluating the state of an organism on the basis of non-invasively obtained biological fluid samples

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EA201600076A EA201600076A1 (en) 2015-12-30 2015-12-30 METHOD FOR ESTIMATING THE STATE OF AN ORGANISM BY SAMPLE OF BIOLOGICAL LIQUID, OBTAINED NONINVASIALLY

Publications (1)

Publication Number Publication Date
EA201600076A1 true EA201600076A1 (en) 2017-07-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA201600076A EA201600076A1 (en) 2015-12-30 2015-12-30 METHOD FOR ESTIMATING THE STATE OF AN ORGANISM BY SAMPLE OF BIOLOGICAL LIQUID, OBTAINED NONINVASIALLY

Country Status (2)

Country Link
EA (1) EA201600076A1 (en)
WO (1) WO2017116277A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL440574A1 (en) * 2022-03-07 2023-09-11 Uniwersytet Medyczny W Białymstoku Salivary biomarker for use in diagnosis, differentiation and neuropsychological assessment of stroke patients

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2783536A1 (en) * 2009-12-09 2011-06-16 Aviir, Inc. Biomarker assay for diagnosis and classification of cardiovascular disease
RU2535025C2 (en) * 2012-10-10 2014-12-10 Федеральное государственное бюджетное учреждение "Научно-исследовательский институт фармакологии" Сибирского отделения Российской академии медицинских наук Method of individual quantitative assessment of risk of hypertension development

Also Published As

Publication number Publication date
WO2017116277A1 (en) 2017-07-06

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