EA201492266A1 - Способ определения чувствительности микроорганизмов к химическим агентам - Google Patents

Способ определения чувствительности микроорганизмов к химическим агентам

Info

Publication number
EA201492266A1
EA201492266A1 EA201492266A EA201492266A EA201492266A1 EA 201492266 A1 EA201492266 A1 EA 201492266A1 EA 201492266 A EA201492266 A EA 201492266A EA 201492266 A EA201492266 A EA 201492266A EA 201492266 A1 EA201492266 A1 EA 201492266A1
Authority
EA
Eurasian Patent Office
Prior art keywords
microorganisms
medium
liquid nutrient
supernatant
nutrient medium
Prior art date
Application number
EA201492266A
Other languages
English (en)
Other versions
EA028467B1 (ru
Inventor
Вадим Говорун
Елена Илина
Алексей Подоплелов
Original Assignee
Идмик Са
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Идмик Са filed Critical Идмик Са
Publication of EA201492266A1 publication Critical patent/EA201492266A1/ru
Publication of EA028467B1 publication Critical patent/EA028467B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Изобретение относится к способу определения чувствительности микроорганизмов к химическим агентам или антибиотикам, заключающемуся в том, что микроорганизмы культивируют в жидкой питательной среде с химическим агентом или антибиотиком, затем среду обрабатывают таким образом, что может произойти лизис клеток и распределение фрагментов микроорганизмов по объему среды, а затем в не содержащем микроорганизмов супернатанте измеряют содержание белковых компонентов и сравнивают значения с контрольными или стандартными образцами. Метод включает добавление некоторого количества микробных клеток к жидкой питательной среде, в которую добавлен усиливающий агент, специфичный для определенной группы (рода или семейства) микроорганизмов. Жидкую питательную среду, содержащую микроорганизмы, преинкубируют, а затем, после добавления некоторого количества антибиотика, снова инкубируют. Затем клетки удаляют из инкубационной среды, получая супернатант. Белки супернатанта экстрагируют добавлением спирта с последующим выделением белков. Белковый осадок используют для получения масс-спектра, используемого для сравнения со стандартными масс-спектрами.
EA201492266A 2012-06-06 2013-06-06 Способ определения чувствительности микроорганизмов к химическим агентам EA028467B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH7852012 2012-06-06
PCT/EP2013/061719 WO2013182647A2 (en) 2012-06-06 2013-06-06 Method for detecting susceptibility of microorganisms to chemical agents

Publications (2)

Publication Number Publication Date
EA201492266A1 true EA201492266A1 (ru) 2015-05-29
EA028467B1 EA028467B1 (ru) 2017-11-30

Family

ID=48577060

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201492266A EA028467B1 (ru) 2012-06-06 2013-06-06 Способ определения чувствительности микроорганизмов к химическим агентам

Country Status (4)

Country Link
US (1) US9376705B2 (ru)
EP (1) EP2859115B1 (ru)
EA (1) EA028467B1 (ru)
WO (1) WO2013182647A2 (ru)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006021493B4 (de) * 2006-05-09 2010-04-01 Bruker Daltonik Gmbh Massenspektrometrische Messung mikrobieller Resistenzen
GB0610009D0 (en) * 2006-05-19 2006-06-28 Health Prot Agency Culture medium
EP2267459A1 (en) 2009-06-25 2010-12-29 Universite Pierre Et Marie Curie - Paris VI Method for determining the susceptibility of a cell strain to drugs
DE102010023452B4 (de) 2010-06-11 2012-11-08 Bruker Daltonik Gmbh Massenspektrometrische Messung von β-Lactamase-Resistenzen

Also Published As

Publication number Publication date
EA028467B1 (ru) 2017-11-30
EP2859115A2 (en) 2015-04-15
WO2013182647A3 (en) 2014-04-10
US9376705B2 (en) 2016-06-28
EP2859115B1 (en) 2017-01-04
US20150191764A1 (en) 2015-07-09
WO2013182647A2 (en) 2013-12-12

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Legal Events

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KG TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): KZ

PC4A Registration of transfer of a eurasian patent by assignment