EA201201629A1 - The method of activation of stem cell proliferation and enhancement of their resistance to adverse effects - Google Patents
The method of activation of stem cell proliferation and enhancement of their resistance to adverse effectsInfo
- Publication number
- EA201201629A1 EA201201629A1 EA201201629A EA201201629A EA201201629A1 EA 201201629 A1 EA201201629 A1 EA 201201629A1 EA 201201629 A EA201201629 A EA 201201629A EA 201201629 A EA201201629 A EA 201201629A EA 201201629 A1 EA201201629 A1 EA 201201629A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- cells
- stem cells
- human
- increase
- magnetic field
- Prior art date
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Изобретение относится к области биотехнологии, медицины, фармакологии, клеточной биологии и биоинженерии и к другим отраслям биологии и медицины, в которых используются стволовые и прогениторные клетки разного уровня дифференцировки и зрелые дифференцированные клетки различных тканей, в частности, для ускоренного создания биоимплантов из донорских или аутоиммунных стволовых клеток человека и/или животных. Техническим результатом, на получение которого направлено изобретение, является активация пролиферации стволовых клеток и повышение устойчивости к неблагоприятным воздействиям стволовых клеток человека и животных, не связанное с негативным воздействием высокочастотного электромагнитного поля. Технический результат достигается с помощью обработки культуры клеток слабым магнитным полем низкой частоты, коллинеарным постоянному магнитному полю Земли. При осуществлении способа наблюдается 1) увеличение количества стволовых клеток человека в культуре после их экспозиции в слабом магнитном поле в течение характерного для данной культуры клеток полупериода удвоения, например за 24 ч количество клеток возрастает более чем в 2, 5 раз (при полном периоде удвоения взятых интактных клеток, равном 48 ч); 2) повышение амплитуды собственного магнитного излучения первичной культуры стволовых клеток, измеряемое магнитометром типа СКВИД, свидетельствует об их возросшей активности; 3) повышение в 2 раза устойчивости стволовых клеток человека относительно развития апоптоза и синхронизация клеток преимущественно в фазе G1 клеточного цикла.The invention relates to the field of biotechnology, medicine, pharmacology, cell biology and bioengineering and to other branches of biology and medicine that use stem and progenitor cells of different levels of differentiation and mature differentiated cells of various tissues, in particular, to accelerate the creation of bioimplants from donor or autoimmune human and / or animal stem cells. The technical result, to which the invention is directed, is the activation of stem cell proliferation and increase of resistance to the adverse effects of human and animal stem cells, not associated with the negative effects of high-frequency electromagnetic fields. The technical result is achieved by treating a cell culture with a weak low-frequency magnetic field collinear with the Earth’s constant magnetic field. When implementing the method, there is 1) an increase in the number of human stem cells in culture after they are exposed in a weak magnetic field during a doubling period characteristic of a given cell culture, for example, in 24 hours, the number of cells increases by more than 2.5 times (with a full doubling period intact cells, equal to 48 h); 2) the increase in the amplitude of the intrinsic magnetic radiation of the primary culture of stem cells, measured by a SQUID type magnetometer, indicates their increased activity; 3) a 2-fold increase in the stability of human stem cells with respect to the development of apoptosis and the synchronization of cells primarily in the G1 phase of the cell cycle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EA201201629A EA019365B1 (en) | 2012-10-02 | 2012-10-02 | The method of boosting stem cell proliferation and increasing their resistance to the adverse effects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EA201201629A EA019365B1 (en) | 2012-10-02 | 2012-10-02 | The method of boosting stem cell proliferation and increasing their resistance to the adverse effects |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA201201629A1 true EA201201629A1 (en) | 2014-02-28 |
| EA019365B1 EA019365B1 (en) | 2014-03-31 |
Family
ID=50185124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201201629A EA019365B1 (en) | 2012-10-02 | 2012-10-02 | The method of boosting stem cell proliferation and increasing their resistance to the adverse effects |
Country Status (1)
| Country | Link |
|---|---|
| EA (1) | EA019365B1 (en) |
-
2012
- 2012-10-02 EA EA201201629A patent/EA019365B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EA019365B1 (en) | 2014-03-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ KG TJ TM |
|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): BY KZ RU |