EA200700942A1 - METHOD FOR DETERMINING THE QUALITY OF CELLULAR CULTURE - Google Patents

METHOD FOR DETERMINING THE QUALITY OF CELLULAR CULTURE

Info

Publication number
EA200700942A1
EA200700942A1 EA200700942A EA200700942A EA200700942A1 EA 200700942 A1 EA200700942 A1 EA 200700942A1 EA 200700942 A EA200700942 A EA 200700942A EA 200700942 A EA200700942 A EA 200700942A EA 200700942 A1 EA200700942 A1 EA 200700942A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cells
quality
masses
cell culture
surface proteins
Prior art date
Application number
EA200700942A
Other languages
Russian (ru)
Other versions
EA009326B1 (en
Inventor
Петр Генриевич ЛОХОВ
Елена Евгеньевна БАЛАШОВА
Original Assignee
Петр Генриевич ЛОХОВ
Елена Евгеньевна БАЛАШОВА
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Петр Генриевич ЛОХОВ, Елена Евгеньевна БАЛАШОВА filed Critical Петр Генриевич ЛОХОВ
Priority to EA200700942A priority Critical patent/EA200700942A1/en
Priority to PCT/RU2007/000571 priority patent/WO2008123793A1/en
Publication of EA009326B1 publication Critical patent/EA009326B1/en
Publication of EA200700942A1 publication Critical patent/EA200700942A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Изобретение относится к области клеточных технологий, а именно к анализу культивируемых in vitro клеток, и может быть использовано в биотехнологии, косметологии, медицине (клеточной трансплантологии), для получения охарактеризованного клеточного материала, обладающего лечебными или косметологическими свойствами. Способ определения качества клеточной культуры включает масс-спектрометрический анализ поверхностных клеточных белков. Предварительно промытые живые клетки исследуемой клеточной культуры подвергают витальному воздействию протеазы, отбирают отщепленные фрагменты поверхностных белков и масс-спектрометрически определяют их массы. Контролируют наличие качественных характеристик у исследуемой культуры клеток путем сравнения совокупности полученных масс с совокупностью масс-спектрометрически определенных масс фрагментов поверхностных белков живых клеток с известными качественными характеристиками. Технический результат, достигаемый при использовании патентуемого изобретения, заключается в повышении объективности (точности) идентификации культивируемых in vitro клеток для отбора качественных (с ожидаемыми полезными свойствами) клеток при снижении временных и материальных затрат.The invention relates to the field of cellular technology, namely the analysis of cultured in vitro cells, and can be used in biotechnology, cosmetology, medicine (cell transplantation), to obtain a characterized cellular material with therapeutic or cosmetic properties. The method for determining the quality of cell culture includes mass spectrometric analysis of cell surface proteins. The pre-washed living cells of the cell culture under study are subjected to the vital effect of protease, the split fragments of surface proteins are selected and their masses are spectrometrically determined. The presence of qualitative characteristics of the studied cell culture is monitored by comparing the aggregate of obtained masses with the aggregate of mass spectrometrically determined masses of fragments of surface proteins of living cells with known qualitative characteristics. The technical result achieved when using the patented invention is to increase the objectivity (accuracy) of the identification of cultured in vitro cells for the selection of high-quality (with the expected useful properties) cells while reducing time and material costs.

EA200700942A 2007-04-06 2007-04-06 METHOD FOR DETERMINING THE QUALITY OF CELLULAR CULTURE EA200700942A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EA200700942A EA200700942A1 (en) 2007-04-06 2007-04-06 METHOD FOR DETERMINING THE QUALITY OF CELLULAR CULTURE
PCT/RU2007/000571 WO2008123793A1 (en) 2007-04-06 2007-10-16 Method for testing a cell culture quality

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EA200700942A EA200700942A1 (en) 2007-04-06 2007-04-06 METHOD FOR DETERMINING THE QUALITY OF CELLULAR CULTURE

Publications (2)

Publication Number Publication Date
EA009326B1 EA009326B1 (en) 2007-12-28
EA200700942A1 true EA200700942A1 (en) 2007-12-28

Family

ID=39831177

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200700942A EA200700942A1 (en) 2007-04-06 2007-04-06 METHOD FOR DETERMINING THE QUALITY OF CELLULAR CULTURE

Country Status (2)

Country Link
EA (1) EA200700942A1 (en)
WO (1) WO2008123793A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2670001C1 (en) * 2017-12-25 2018-10-17 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии растений и микроорганизмов Российской академии наук Method for producing cell surface proteins

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU944580A1 (en) * 1980-02-07 1982-07-23 Кировский научно-исследовательский институт переливания крови Method of obtaining surface b-hepatitis antigen
PT1000154E (en) * 1997-07-23 2007-03-30 Roche Diagnostics Gmbh Identification of human cell lines for the production of human proteins by endogenous gene activation
JP2001249125A (en) * 2000-03-06 2001-09-14 Suntory Ltd Method for analysis of substance in tissue or cell
US6861234B1 (en) * 2000-04-28 2005-03-01 Mannkind Corporation Method of epitope discovery
CA2460568A1 (en) * 2001-09-21 2003-03-27 Caprion Pharmaceuticals Inc. Preparation of highly-purified plasma membranes
AU2003902299A0 (en) * 2003-05-13 2003-05-29 Flinders Medical Centre A method of analysing a marker nucleic acid molecule
US7164552B2 (en) * 2003-09-29 2007-01-16 Hitachi Global Storage Technologies Netherlands B.V. Method for self-servo writing a disk drive with dual-stage actuator
CA3218940A1 (en) * 2004-11-12 2006-05-18 Cambridge University Technical Services Ltd. Methods and means related to cancer stem cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2670001C1 (en) * 2017-12-25 2018-10-17 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии растений и микроорганизмов Российской академии наук Method for producing cell surface proteins

Also Published As

Publication number Publication date
EA009326B1 (en) 2007-12-28
WO2008123793A1 (en) 2008-10-16

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

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NF4A Restoration of lapsed right to a eurasian patent

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU

NF4A Restoration of lapsed right to a eurasian patent

Designated state(s): RU

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU

NF4A Restoration of lapsed right to a eurasian patent

Designated state(s): RU