EA043989B1 - SYNERGISTIC HERBICIDE COMPOSITION OF A WIDE SPECTRUM OF ACTION FOR WEED CONTROL OF AGRICULTURAL CROPS, ITS APPLICATION AND METHOD OF ITS APPLICATION - Google Patents

Description

Field of invention

The present invention relates to a solution that brings significant benefits to the agricultural sector, with a wide range of applications as an aid in the cultivation of all types of plants of agricultural interest, for example the cultivation of rice, corn, sorghum, wheat, barley, oats, cereals, triticale , soybeans, beans (and their varieties such as beans), cotton, fruits (and their varieties such as peaches, apples, pineapples and tomatoes), potatoes, sweet potatoes, canola, flax, peas, lentils, mustard, chickpeas, sunflowers, alfalfa, onions, herbs, sugar cane, beets, saccharin, turmeric, cassava, pumpkin, etc.

This list of plants of agricultural interest is not intended to limit the wide range of uses and may be extended to include all plant species classified as such, reasonably known to the art or likely to be cataloged in the future.

The present invention relates to a synergistic broad-spectrum herbicide composition for the control of weeds of agricultural crops, such as those mentioned above, containing at least two herbicides, which are a herbicide belonging to the group, the mechanism of action of which is a photosystem II inhibitor, in a combination of at least at least one herbicide belonging to a group whose mechanism of action is as an inhibitor of the enzyme protoporphyrinogen oxidase.

State of the art

To demonstrate the validity and clarify the context described in the introductory section, a brief explanation of the state of the art for pesticide products, particularly herbicides, will be provided, providing insight from those skilled in the art regarding their technical impact on crops that benefit from their use, and restrictive aspects of this agricultural management practice, thereby establishing the range of requirements previously described in the section regarding the need for the present invention.

(a) The yield of crops of interest is determined by several factors, mainly:

variety of crop of interest;

soil (physical, chemical and biological properties);

climate of the region;

pest control and weed control.

For the purposes of the present invention, it is appropriate to focus on the weed control factor in general, i.e. consider the full range of weeds that affect each crop species of interest.

By way of example only, and without any limitation, a list of some weeds responsible for reducing crop yields is presented in Table 1. 1.

Table 1

Some weeds found in crops

Scientific name Popular name Brachiaria plantaginea Vetvyanka Panicum maximum Large millet Brizantha Brachiaria Grass picket fence Cenchrus echinatus Cenchrus pauciflora Amaranthus sp Schiritsa spiny Portufaca oleracea Purslane Cleomis affinis Cleome spiny

(b) Use of herbicides to control crop weeds: This is one of the most effective ways of controlling weeds without damaging the crops of interest. As used herein, herbicide means an active ingredient that kills, controls or adversely affects plant growth. It can also cause deviations from natural development, death, regulation, drying out, developmental delay, etc. Meanwhile, the herbicidal effect is carried out through the chemical compounds present in the composition when they are applied directly to the plant or to a section of the plant in any phase of growth, before planting or during the growth process. In addition, the observed effect depends on several factors, such as the plant species controlled, the stage of plant growth, application conditions of the composition, taking into account the droplet and particle size of the components, as well as other factors such as environmental conditions

- 1 043989 media, adjuvants, excipients and carriers used, soil type, and the quantity and quality of the chemical product used.

To be successful in weed control, it is necessary to have in-depth knowledge of factors such as: the physiology of herbicides in the plant, factors influencing selectivity and behavior of herbicides in the soil.

(c) Mechanisms of action of herbicides: These are defined as the first chemical or physical reaction that occurs within the cell of the weed and which results in a change in its growth, the main known mechanisms being listed below:

(p. 1) growth regulators or auxin mimetics: they have a greater effect on dicotyledonous weeds; cause plant growth disorders by affecting meristematic tissues; exhibit the transfer of substances predominantly through symplast;

(p. 2) mitosis and initial growth inhibitors: the dinitroaniline group has characteristics such as control of most grasses; does not have post-emergence effect; absorbed by stems and roots; does not exhibit substance transfer; does not fight perennial weeds and acts by suppressing cell division;

(p. 3) inhibitors of amino acid synthesis: in particular, inhibitors of the ALS enzyme, they promote aposymplastic transfer of substances; EPSPS enzyme inhibitors that suppress the synthesis of the amino acids phenylalanine, tyrosine and tryptophan; they are not selective; fight dicotyledonous weeds and grasses, symplastic transfer of substances;

(p. 4) pigmentation inhibitors: they act on the biosynthesis of carotenoids, producing albino tissue; loss of chlorophyll occurs due to oxidation under the influence of light (photooxidation), which occurs as a result of a lack of carotenoids that protect them; transfer is apoplastic;

(p. 5) respiration inhibitors: they exhibit symplast-limited transport of substances; used for post-emergence treatments; mainly fight against herbs; high temperature and illumination increase their effectiveness;

(p. 6) membrane disruptors: they inhibit the enzyme protoporphyrinogen oxidase (PROTOX); signs of which are dark green spots on the leaves, turning into necrosis; they exhibit reduced apoplastic transport of substances; and (p. 7) photosystem II inhibitors: this group includes triazines, triazinones, substituted ureas and uracils. Triazines are commonly used in pre-emergent or early post-emergent applications and are effective on dicotyledonous weeds and some grasses and exhibit apoplastic transport.

(d) Scope of Chemical Groups by Mechanism of Action: For the purposes of the present invention, only photosystem II inhibitory mechanisms and protoporphyrinogen oxidase (PROTOX) enzyme inhibitors, membrane disruptors, which include herbicides of the chemical group triazolinones and at least one herbicide selected from the group of cyclohexene dicarboximides, are considered. respectively.

(d.1) PSII Photosynthesis Inhibitor Group: For the purposes of the present invention, amicarbazone has been selected as the active ingredient belonging to this mechanism of action group, and it is necessary to further consider the active ingredient amicarbazone in all its forms and variations.

(d.1.1) Active ingredient amicarbazone: its technical characteristics are presented below in the preferred form of implementation in table. 2.

table 2

Amicarbazone - technical characteristics

Formula C10H19N5O2 Compound 4-amino-X-(tert-butyl)-3-isopropyl-5-oxo-4,5-dihydro-1H-1,2,4triazol-1-carboxamide Connections [other] 4-amino-A-tert-butyl-5-oxo-3-(propan-2-yl)-4,5-dihydro-1 Η-1,2,4- triazole-1-carboxamide 4-amino-M-tert-butyl-4,5-dihydro-3-isopropyl-5-oxo-1H-1,2,4- triazole-1-carboxamide 4-amino-M-(1,1-d imethylethyl)-4,5-dihydro-3-(1-methylethyl)-5-oxo-1H1,2,4-triazole-1-carboxamide Action Inhibition of photosynthesis II

(d.1.2) Criticality analysis: despite the fact that its properties have been confirmed over the past decades, the herbicide amicarbazone, selected from the group of triazolinones and, in particular, belonging to the group of photosynthesis inhibitors PSII, when used alone, has shown limited effectiveness. significant results demonstrating to those skilled in the art reduced control of the target plant, a shorter residual period and a reduced list of weeds controlled simultaneously.

This decrease in the effectiveness of the herbicide amicarbazone can be explained by the fact that the intrinsic activity of this active ingredient does not provide effective control of the entire list of weeds.

Finally, it can be argued that the situation of progressive loss of effectiveness of the active ingredient amicarbazone can be extended to all active ingredients belonging to the mechanism of action group that includes photosynthesis inhibitors II.

(d.2) Group of PROTOX inhibitors: also called protoporphyrinogen oxidase enzyme inhibitors, where for the purposes of the present invention the active ingredient flumioxazin from the chemical group cyclohexene dicarboximides has been selected, belonging to this group of mechanism of action and should be considered in all forms and variations.

(d.2.1) Active ingredient flumioxazin: its technical characteristics are presented below in one preferred form of implementation in table. 3.

Table 3

Flumioxazin - Technical characteristics

Formula C ₁₉ H ₁₅ FN ₂ O4 Compound N(7-φτορ-3,4-dihydro-3-oxo-4-prop-2-ynyl-2H-1,4benzoxazin-6-yl) cyclohex-1-ene-1,2-dicarboximide Action Inhibition of protoporphyrinogen oxidase (PPO)

(d.2.2) Criticality analysis: Although its properties have been confirmed over the past decades, the herbicide flumioxazin, selected from the group of cyclohexene dicarboximides and, in particular, belonging to the group of protoporphyrinogen oxidase enzyme inhibitors [PROTOX], used alone, has shown limited results demonstrating to those skilled in the art reduced control of the target weed, a shorter residual period, and a reduced list of weeds controlled simultaneously.

This decrease in the effectiveness of the herbicide flumioxazin can be explained by the fact that the intrinsic activity of this active ingredient does not provide effective control of the entire list of weeds.

Finally, it can be argued that the situation of progressive loss of effectiveness of the active ingredient flumioxazin can be extended to all active ingredients belonging to the mechanism of action group that inhibits the enzyme protoporphyrinogen oxidase [PROTOX].

Below are some principles of the prior art that are relevant to the present subject.

Patent documents EP-A-294666, EP-A-370293, EP-A-391187, EP-A-398096, EP-A-399294, EP-A-415196 and EP-A-477646 describe the process of obtaining and/or applications of carbamoyl triazolinones, for example, where the active ingredient is always considered in isolation.

Meanwhile, patent documents PI 9603223-5 and PI 9603448-3 disclose a method for producing substituted aminocarbonyl triazolinones and substituted aminotriazolinones, respectively, also separately.

In addition, patent documents JP 61-76486 A and JP 5-97848 describe a method for producing flumioxazin.

Patent document BR 112014019707-5 relates to a crystal of flumioxazine, where the active ingredient is also always listed separately.

There are also patent documents that disclose combinations or compositions containing one of the two active ingredients mentioned above, as well as uses/methods, such as BR 1020170233138, BR 1020160077672, BR 1120170156520, BR 1020130090239, BR 1120130181141, PI 041308 2-0, PI 0302668-0, PI 0211275-2, PI 0015670-1, PI 9704565-9, PI 9509362-1 and PI 92059999-6.

The results of individual application of both the active ingredient amicarbazone and flumioxazin, according to specialists in the field of crop management with an emphasis on the use of herbicides, lead to the first and correct conclusion that the resistance of weeds to them is due to the fact that they have acquired a reduced sensitivity to the mechanisms actions such as photosynthesis inhibitors PSII and protoporphyrinogen oxidase enzyme inhibitors [PROTOX], respectively.

As a result, given that the mechanisms of action described previously are the reason for the limited action of the active ingredients amicarbazone and flumioxazin, it can be concluded that this effect applies to all herbicides whose active ingredients belong to the same groups of mechanisms of action.

- 3 043989

Thus, in the current state of the art there is no solution equivalent to that presented in the present invention, which would combine technical distinguishing factors, economic advantages, safety and reliability.

Terminology

For a better understanding of the present invention, the meaning of certain terms cited throughout the specification is set forth below.

Commodity: A term used primarily to refer to basic products in raw or slightly industrialized form, of nearly uniform quality, produced in large quantities by different manufacturers. These natural products can be stored in a warehouse for a certain period without significant loss of quality, depending on their preservation, and are subject to global quotation and trading using commodity exchanges. For the purposes of the present invention, a plant of agricultural interest is a commodity.

Synergy: In general, this term can be defined as a combination of two elements of such a kind that the result of this combination is greater than the sum of the results that these elements would have separately. This is an unexpected result, achieved only through the selection of components, and therefore cannot be predicted, inferred or assumed.

Plants: Includes germinating seeds, cuttings, emerging seedlings, and established vegetation, including roots and aerial parts such as leaves, stems, flowers, fruits, branches, limbs, roots, and the like.

Plant of agricultural interest: should be understood as any type of plant intended for commercial consumption, which may be edible or inedible, floral or non-floral, trees, grasses [plants with creeping rhizomes, herbaceous plants or herbs].

Weed: According to Lorenzi (2014) is any plant that grows where it is undesirable, directly or indirectly affecting crops of interest, causing a significant reduction in the overall yield of those crops.

Biotic factors: This term can be interpreted as the sum of all the effects caused by organisms in an ecosystem that determine the populations that form it, and in the context of the present invention, organisms are considered to be weeds.

Agrochemicals: Also known as insecticides, pesticides, biocides, plant pharmaceuticals or plant health products, are general terms for various chemicals used in agriculture. The World Health Organization (WHO) defines a pesticide or insecticide as any substance capable of controlling pests that may pose a risk or cause harm to populations and the environment. They may also be defined as substances or mixtures of substances intended to prevent the effects of or directly kill insects (insecticides), mites (acaricides), shellfish (molluscicides), rodents (rodenticides), fungi (fungicides), weeds (herbicides), bacteria ( antibiotics and bactericides) and other species of animals or plants harmful to public health and agriculture.

Herbicide: is a type of pesticide, a chemical product used in agriculture to control grasses classified as weeds.

Herbicide of interest: any formulation of a commercially known herbicide for which the technical effects of use need to be improved and the harmful effects of which need to be minimized.

Active Ingredient: For the purposes of the present invention, defined as the main chemical substance of the herbicide.

Ha: hectare, a unit of measurement of agricultural land corresponding to one hundred acres or square hectometer (10,000 m ² ).

Colby Method: One of the most widely used methods for demonstrating synergy between compounds, this invention is used to calculate the expected response between herbicides of interest.

Herbicide Resistance Action Committee: a group of industry-related experts recognized as an advisory body by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization of the United Nations (WHO).

The list of terms, technologies and technical concepts presented in this section should be considered for a proper understanding of the present invention, giving the necessary descriptive sufficiency to the text of the description, and should be used as reference material for comparative analysis studies or for hypothetical prior art solutions that anticipate invention and are not mentioned herein, or for products of the same nature and the same international patent classification (CPI), disclosed and/or sold by the owners or third parties who are not the owners of this patent.

- 4 043989

Objectives of the invention

It is therefore an object of the present invention to provide a herbicidal composition that satisfies the need to enhance the effects of herbicide-type pesticides, taking into account the inevitable fact that crop plants of interest are susceptible to biotic factors.

Another object of the present invention is to provide a herbicidal composition capable of controlling a wide range of weeds.

Another object of the present invention is to provide a herbicidal composition containing herbicides already known in the state of the art, which gives a better result than those currently known, since the development of new molecules is becoming increasingly difficult and expensive.

It is yet another object of the present invention to provide a herbicidal composition containing herbicides already known in the art, which gives a better result than currently known, due to the progressive deterioration in the effectiveness of the individual photosynthesis inhibitor herbicides PSII and the enzyme protoporphyrinogen oxidase [PROTOX ] accordingly, well known.

In addition, another object of the present invention is to provide a herbicidal composition containing known components, even having a limited number of alternative herbicides for use in the control of resistant biotypes (the number of active ingredients available for the control of some weed species is limited).

Moreover, another object of the present invention is to provide a herbicidal composition that restores effectiveness close to that recorded during the first use of both PSII photosynthesis inhibitory herbicides and protoporphyrinogen oxidase enzyme inhibitors [PROTOX], respectively, i.e. demonstrates close to 100% effectiveness in killing all types of weeds in a wide variety of crops of interest.

Another object of the present invention is to provide a herbicidal composition that provides benefits from an agro-industrial, commercial and technical point of view.

It is also an object of the present invention to assist in increasing the yield of a crop of interest, particularly with regard to effectiveness in controlling biotic factors.

It is further an object of the present invention to obtain the synergistic effect of restoring the effectiveness of herbicides as a photosystem II inhibitor in combination with restoring their effectiveness as inhibitors of the enzyme protoporphyrinogen oxidase [PROTOX].

The essence of the invention

The present invention achieves these and other objects by providing a synergistic, broad-spectrum herbicidal composition for the control of crop weeds, which contains:

an amount ranging from 1.0 to 99.0% by weight of a first herbicide belonging to the group whose mechanism of action is a photosystem II inhibitor, being amicarbazone;

an amount ranging from 1.0 to 99.0% by weight of a second herbicide belonging to the group whose mechanism of action is as an inhibitor of the protoporphyrinogen oxidase enzyme, which is flumioxazin;

acceptable medium;

amounts are based on the total weight of the herbicidal composition.

In a preferred embodiment, the ratio by weight between the first herbicide belonging to the group whose mode of action acts as a photosystem II inhibitor and the second herbicide belonging to the group whose mode of action acts as an inhibitor of the enzyme protoporphyrinogen oxidase is from 5 to 6 parts per 1 part .

In yet another preferred embodiment, said herbicidal composition contains 700 g of amicarbazone and 125 g of flumioxazin in a.i./ha.

Moreover, the present invention achieves these and other objects by using said herbicidal composition to control weeds present in crops, as well as by a method of using said herbicidal composition, which includes the following steps:

a) selection of crop site;

b) applying a sufficient effective amount of said composition to the plants and/or directly to the environment/site/soil.

Disclosure of the invention

In experiments with growing agricultural crops, the need was identified to increase the impact of herbicide-type pesticides, taking into account the inevitable fact that the crops of interest

- 5 043989 agricultural plants are exposed to biotic factors.

(a) Motivation for developing the present invention: It is generally well known to those skilled in the field of crop management that if, on the one hand, the use of herbicides contributes to the effective control of weeds leading to a reduction in productivity with loss of yield per area [hectare] of plantation , on the other hand, it has been discovered that the list of plants that have become resistant to this class of pesticides is constantly growing.

VARGAS, L; ROMAN, E.S. in the publication of a study entitled Weed resistance to herbicides: concepts, origin and evolution, Passo Fundo: Embrapa Trigo, 2006. 22 p. html. (Embrapa Trigo. Documents Online, 58), argue that [...] Herbicide resistance in weeds is of great importance, mainly due to the limited number of alternative herbicides available for use to control resistant biotypes. The number of active ingredients available to control some weed species is limited, and the development of new molecules is becoming increasingly difficult and expensive. The emergence of multiple resistance further compounds the problem because two or more mechanisms must be replaced. Thus, control of resistant biotypes through the use of herbicides is compromised, limiting this practice to other less effective methods.

In accordance with the need of the present invention, a synergistic broad-spectrum herbicide composition has been developed for the control of crop weeds, containing at least one herbicide belonging to a group whose mode of action is a photosystem II inhibitor in combination with at least one herbicide, belonging to the group whose mechanism of action is as an inhibitor of the enzyme protoporphyrinogen oxidase, the object of the present invention. This composition does not appear or appear to be derived from other combination-based herbicide management techniques for the cultivation of crop species of interest envisaged by the state of the art to provide advantages from an agro-industrial, commercial and technical point of view.

Notably, the present invention provides an innovative composition comprising in an ingenious manner at least one herbicide belonging to a group whose mode of action is a photosystem II inhibitor in combination with at least one herbicide belonging to a group whose mode of action is an enzyme inhibitor protoporphyrinogen oxidase.

Additionally, surprisingly, an unexpected synergistic effect was observed following treatment of crops of interest with a herbicidal composition consisting of at least one herbicide belonging to a group whose mode of action is a photosystem II inhibitor in combination with at least one herbicide , belonging to a group whose mechanism of action works as an inhibitor of the enzyme protoporphyrinogen oxidase, particularly in the Colby method used to demonstrate control synergy.

It is also worth noting the originality of the use of this synergistic herbicide composition on weeds.

(a) Synergistic herbicidal composition.

(a.1) The basic combination for the purposes of the present invention is defined by the following formula:

C = A + B, where C = a combination of at least one herbicide [A] belonging to the group, the mechanism of action of which is a photosystem II inhibitor, in combination with at least one herbicide belonging to the group, the mechanism of action which works as both an inhibitor of the enzyme protoporphyrinogen oxidase and membrane disruptors [PROTOX];

A = a herbicide belonging to a group whose mode of action is as a photosystem II inhibitor, selected from the list of active ingredient groups and corresponding chemical groups given in Table. 4.

- 6 043989

Herbicide Opportunity Range [A]

Table 4

Chemical group Active Ingredient Triazolinones Amicarbazone Triazines Ametrine Atrazine Desmethrin Propazine Terbutylazine Simethrine Dimetameter in Prometon Terbutrine Simazine Cyanazine Prometrine Terbu meton Triethazine Triazinones Hexazinone Metamitron Metribuzin Urea Chlorobromuron Fenuron Metobromuron Neburon Chlorotoluron Fluometuron Metabenzthiazuron Siduron Chloroxuron Isoproturon Metoxuron Isouron Dimefuron Monolinuron

- 7 043989

Etidimuron Diuron Linuron Tebuthiuron Uracils Bromacil Lenacil Terbacil Pyridazinones Chloridazone/pyrazone Phenylcarbamates Desmediphs Phenmedipham Amides Pentanochlor Propanil Nitriles Bromophenoxime Bromoxynil Ioxynil Phenylpyridazines Pyridate Pyridafol Benzothiadiazinones Bentazon

List of chemical groups and their active ingredients listed in Table. 4 is not exhaustive, and for the purposes of the present invention, any and all herbicides should be considered, the active ingredient and the corresponding chemical group of which belongs or can be classified as belonging to the group whose mechanisms of action inhibit photosystem II, according to the classification of the Committee on the Prevention of Resistance to Action herbicides [HRAC].

B = a herbicide belonging to a group whose mode of action is as an inhibitor of the enzyme protoporphyrinogen oxidase [PROTOX], selected from the list of active ingredient group and corresponding chemical groups given in Table. 5.

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Table 5

Herbicide Opportunity Range [B]

Chemical group Active Ingredient Diphenyl ethers Acifluorfen sodium Bif enox Etoxif enethyl Halosaphen Chlomethoxifen Fluoroglycophene-ethyl Fomesafen Lactofen Oxyfluorfen Phthalimids Flumichlorac Flumioxazin Cinidone-ethyl Flumichlorac-pentyl Triazolinones Carfentrazone-ethyl Sulfentrazone Azaphenidine Bencarbazone Oxadiazoles Oxadiazon Oxadiargyl Pyrimidinediones Saflufenacil Benzfendizone Butafenacil Phenylpyrroles Flu azolate Pyraflufen-ethyl Thiadiazoles Flutiacet-methyl Thidiazimine Oxazolidinediones Pentoxazone Others Flufenpyr-ethyl Pyraclonil Profluazole

List of chemical groups and their active ingredients listed in Table. 5 is not exhaustive, and for the purposes of the present invention it covers any herbicide the active ingredient and corresponding chemical group of which is or can be classified as belonging to the group whose mechanisms of action inhibit the enzyme protoporphyrinogen oxidase [PROTOX], as classified by the Committee on the Prevention of Resistance. action of herbicides [HRAC].

Thus, the present invention relates to a synergistic herbicidal composition containing:

an amount ranging from 1.0 to 99.0% by weight of a first herbicide belonging to the group whose mechanism of action is a photosystem II inhibitor;

an amount ranging from 1.0 to 99.0% by weight of a second herbicide belonging to the group whose mode of action is as an inhibitor of the enzyme protoporphyrinogen oxidase;

acceptable medium; wherein the amounts are based on the total weight of the herbicidal composition.

Preferably, the synergistic herbicidal composition of the present invention contains from 5 to 6 parts by weight of a first herbicide belonging to a group whose mode of action is a photosystem II inhibitor to one part by weight of a second herbicide belonging to a group whose mode of action is an inhibitor. the enzyme protoporphyrinogen oxidase, and a suitable carrier, the amounts being based on the total weight of the herbicidal composition.

Together, these herbicides enhance their respective mechanisms of action and therefore increase their effectiveness in killing weeds.

The components of the synergistic composition of the present invention can be used individually or as part of a multi-element herbicide system.

In addition, the composition that is the object of the present invention can be used in

- 9 043989 combination with one or more other herbicides and/or biocides to control a wider variety of unwanted vegetation. Preferably, a herbicide or biocide is added to the composition of the present invention, the herbicides being selective for the crops being treated and complementing the spectrum of weeds controlled by the compounds at the application rate used. Some of the herbicides that may be used in combination with the synergistic composition of the present invention include, but are not limited to, propisochlor, smetolachlor, acetochlor, cleethodim, chisalofop, diquat, glufosinate ammonium, pyroxasulfone, imazethapyr, diclosfulam, saflufenacil, glyphosate, 2,4 -D, triclopyr, dicamba, atrazine, chlorimuron, diuron, sulfentrazone, isoxaflutole, picloram, tebuthiuron, tembotrione, mesotrione.

In preferred embodiments, the first herbicide belonging to the group whose mechanism of action works as a photosystem II inhibitor is amicarbazone, and the second herbicide belonging to the group whose mode of action works as an inhibitor of the enzyme protoporphyrinogen oxidase is flumioxazine.

Optionally, the composition which is the subject of the present invention preferably contains water as a carrier.

The synergistic herbicidal composition, which is the object of the present invention, can be used to obtain herbicidal products. Thus, herbicidal products contain the composition that is the object of the present invention, and other optional components, such as:

herbicides/biocides/pesticides/larvicides/fungicides/insecticides;

herbicidal protectors;

carriers and solvents;

oils;

emulsifiers/surfactants;

compatibility agents;

antifoaming agents;

sequestering agents;

neutralizing agents and buffers;

corrosion inhibitors;

dyes;

flavorings;

dispersing agents;

means of penetration;

adhesives;

dispersing agents;

thickeners;

freezing point depressants;

antimicrobial agents;

plant growth regulators;

other components commonly used in herbicide products.

The present invention also relates to a method of using the synergistic herbicidal composition, which is the object of the present invention, for the control of weeds in plantations.

This method includes the following steps:

A) selection of an agricultural plantation site;

B) applying a sufficient effective amount of the herbicidal composition of the present invention or herbicidal product of the present invention to each plant or environment.

The composition can be applied to any vegetative part of plants present in the crop, either directly or by allowing the compounds to act in the environment. Application of the composition or finished product can be accomplished in a variety of ways, such as dipping, spraying, evaporating, atomizing, applying directly to seeds, as well as applying directly to the soil, to parts of leaves, and to straw on the soil, among other possibilities.

(a.2) Preferred Embodiment: Taking into account the general composition, for the purposes of the present invention, a preferred form of implementation of the herbicidal composition according to the present invention is provided, which will be represented by the derivative formula

C1 = A1 + B1, where C1 = combination of two herbicides amicarbazone + flumioxazin;

A1 = active ingredient amicarbazone;

B1 = active ingredient flumioxazin.

(b) Form of Presentation: Any form of presentation should be considered, such as the following: encapsulated suspension, dispersible concentrate, emulsifiable concentrate, water-in-oil emulsion, oil-in-water emulsion, microemulsion, concentrated suspension, emulsion-like,

- 10 043989 soluble granules, soluble concentrate, soluble powder, tablet, direct application tablet, water dispersible tablet, water dispersible tablet, dispersible granules, wettable powder, block, concentrated gel in paste, emulsifiable gel, water soluble gel, emulsifiable granules, emulsifiable powder, oil dispersion or suspension concentrated in oil, dispersible or miscible suspension concentrated in oil, miscible solution in oil, powder dispersible in oil, encapsulated granules, dry powder, electrostatic/electrodynamic spray liquid, granules, Spray/Spread Oil, Ultra Low Volume Suspension, Ultra Low Volume, Micro Granules, Fine Powder, Fine Granules, Contact Powder, Direct Application Tablet, Liquid or Contact Gel, Concentrated Direct Application Suspension, Other Direct Application Liquids, Other Powders, Direct Application Powder dry seed treatment, seed treatment emulsion, concentrated seed treatment suspension, seed treatment solution, soluble seed treatment powder, oil paste powder, water paste powder, encapsulated seed treatment suspension, seed treatment gel , Aerosol, Fumigant, Fumigant liner, fumigant candle, fumigant cartridge, fumigant rod, fumigant pill, fumigant granules, liquefied gas under pressure, gas generator, concentrate for cold spraying, varnish, vegetable rod, pasta, bait, grain bait. , block bait, granular bait, plate bait, waste bait, steam emitter, oil paste, mixture packet, adjuvant, surfactant and sticky surfactant.

(c) Test: In order to confirm the synergism observed between the herbicidal compounds present in the composition that is the object of the present invention, the results of the tests performed are presented below.

Protocol: the composition according to the present invention was added to the prepared soil with weed seeds (without germination). This test was carried out on two weed species: Brachiaria decumbens and Panicum maximum. Data were collected 35 days after application of the composition to the soil and calculated.

(d) Confirmation of synergistic effect: For the purposes of the present invention, verification of synergistic effect is based on two basic principles: the Colby equation and the representation of test results in the above equation, which will be properly described.

(d.1) Colby Equation: To use the Colby method to evaluate synergism or antagonism in combinations, the components of the combination should be tested separately at their component concentrations in the same study as the combination.

The results observed for the combination are compared with the expected results from Colby's equation (Colby, 1967). It should be noted that this equation is derived from the definition of the probability of independent events.

Assuming that the responses vary over a percentage range of 0 to 100% pest reduction, the Colby equation is expressed by the following formula:

Ex + (100 - X)(Y/100) (1)

X + Y - X*Y/100, where X = observed result of the active ingredient [A1] amicarbazone in r grams a.i./ha;

Y = observed result of active ingredient [B1] flumioxazin in q grams a.i./ha;

E = expected result for the combination of active ingredients [A1]amicarbazone and [B1]flumioxazin in (p + q) grams a.i./ha, in the absence of synergism or antagonism.

(d.2) Analysis criteria:

if the observed value is higher than the expected value (Obs > E): synergy;

if the observed value is lower than the expected value (Obs < E): antagonism.

(d.3) Comparative analysis: having table as a research paradigm. 6 presented earlier, the synergism or antagonism analysis for the test is presented in a simplified form below in table. 6 and 7.

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Table 6

Test results - synergistic activity confirmed 35 days after application

Application rate Control % Amicarbazone Flumioxazin PANMA Rate in grams of active ingredient/ha Observable Expected 238.00 0.00 83.25 - 0.00 380.00 97.50 - 238.00 380.00 100.00 99.58 317.30 0.00 75.00 0.00 507.70 98.75 - 317.30 507.70 100.00 99.69 442.00 0.00 0.00 _ 0.00 176.80 97.55 - 442.00 176.80 100.00 99.90 589.30 0.00 90.00 0.00 235.70 99.00 - 589.30 235.70 100.00 99.90 515.60 0.00 90.00 _ 0.00 103.10 98.75 - 515.60 103.10 100.00 99.88 687.50 0.00 95.00 0.00 137.50 97.50 - 687.50 137.50 100.00 99.88 571.15 0.00 91.25 _ 0.00 47.60 89.50 - 571.15 47.60 99.50 99.08 761.54 0.00 98.25 0.00 63.46 97.00 - 761.54 63.46 100.00 99.95 594.00 0.00 86.25 0.00 24.75 81.25 - 594.00 24.75 98.75 97.42 792.00 0.00 88.75 _ 0.00 33.00 88.75 - 792.00 33.00 100.00 98.73

PANMA - Panicum maximum (Guinea grass). grams a.i./ha - grams of active ingredient per 1 ha.

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Claims (5)

Table 7 Test results - synergistic activity was confirmed 35 days after application. Application rate Control % Amicarbazone Flumioxazin BRADC Norm in ingredient/ha grams active Observable Expected 238.00 0.00 52.50 - 0.00 380.00 90.00 - 238.00 380.00 100.00 95.25 442.00 0.00 66.25 0.00 176.80 92.75 - 442.00 176.80 100.00 97.55 515.60 0.00 62.50 0.00 103.10 83.75 - 515.60 103.10 100.00 93.91 571.15 0.00 77.50 0.00 47.60 81.25 - 571.15 47.60 100.00 95.78 BRADC - Brachiaria decumbens, grams a.i./ha - grams of active ingredient per 1 ha. As can be concluded from the results presented above, the synergism between the compounds present in the composition that is the object of the present invention is confirmed for several ratios. The selection of the preferred embodiment of the present invention described in this section is presented by way of example only. Changes, modifications and variations can be made in any other form of the herbicidal composition according to the present invention of at least one herbicide belonging to the group, the mechanism of action of which is as a photosystem II inhibitor in combination with at least one herbicide belonging to the group, mechanism which acts as an inhibitor of the enzyme protoporphyrinogen oxidase, and such changes can be determined by those skilled in the art without deviating from the intent of the claims of this patent, which are defined solely by the appended claims. CLAIM 1. A synergistic broad-spectrum herbicide composition for controlling weeds of agricultural crops, containing: an amount ranging from 1.0 to 99.0% by weight of a first herbicide belonging to the group whose mechanism of action is a photosystem II inhibitor, being amicarbazone; an amount ranging from 1.0 to 99.0% by weight of a second herbicide belonging to the group whose mechanism of action is as an inhibitor of the protoporphyrinogen oxidase enzyme, which is flumioxazin; acceptable medium; amounts are based on the total weight of the herbicidal composition. 2. The herbicidal composition according to claim 1, characterized in that the weight ratio between the first herbicide belonging to the group, the mechanism of action of which acts as an inhibitor of photosystem II, and the second herbicide belonging to the group, the mechanism of action of which acts as an inhibitor of the enzyme protoporphyrinogen oxidase, is 5 to 6 parts to 1 part. 3. Herbicidal composition according to claim 1 or 2, characterized in that it contains 700 g of amicarbazone and 125 g of flumioxazin in ai/ha. 4. Use of a herbicidal composition according to any one of claims 1 to 3 to control weeds present in agricultural crops. 5. Method of using the herbicidal composition according to claim 1, characterized in that it includes the following steps: a) selection of a crop site; b) applying a sufficient effective amount of said composition to the plants and/or directly to the environment/site/soil. Eurasian Patent Organization, EAPO Russia, 109012, Moscow, Maly Cherkassky lane, 2