DK659287A - EXPRESSION OF THE CLONED LYSOSTAPHIN GEN - Google Patents
EXPRESSION OF THE CLONED LYSOSTAPHIN GENInfo
- Publication number
- DK659287A DK659287A DK659287A DK659287A DK659287A DK 659287 A DK659287 A DK 659287A DK 659287 A DK659287 A DK 659287A DK 659287 A DK659287 A DK 659287A DK 659287 A DK659287 A DK 659287A
- Authority
- DK
- Denmark
- Prior art keywords
- lysostaphin
- pjp1
- provides
- pdf8
- prg5
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Abstract
The present invention provides recombinant plasmids which is transformant microbial hosts express lysostaphin, a bacteriocin that kills most known staphylococcal species. The invention also provides lysostaphin, substantially free from non-lysostaphin contaminants. Recombinant plasmids, pRG5, pJP1, pDF8 and pRP1, were derived by inserting a 1.5 kilobase segment of DNA coding for lysostaphin into the cloning vectors, pUC8, pBC16, pBD64 and pSPV1, respectively. E. coli strain JM105 transformed by pRG5 and members of Bacillus species, including B. subtilis and B. sphaericus transformed by pJP1, pDF8 and pRP1 produce lysostaphin which is immunologically and electrophoretically indistinguishable from that produced by S. simulans, the natural source. Furthermore, B. sphaericus strain 00/pJP1 transformants produce five times the amount of lysostaphin as S. simulans. The invention also provides the 1.5 kbp DNA fragment coding for lysostaphin. The sequence of the DNA encodes preprolysostaphin, a monomeric 389 amino acid protein, which is posttranslationally processed to mature lysostaphin.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US85240786A | 1986-04-16 | 1986-04-16 | |
US85240786 | 1986-04-16 | ||
US3446487 | 1987-04-10 | ||
US07/034,464 US4931390A (en) | 1986-04-16 | 1987-04-10 | Expression of the cloned lysostaphin gene |
US8700873 | 1987-04-15 | ||
PCT/US1987/000873 WO1987006264A1 (en) | 1986-04-16 | 1987-04-15 | Expression of the cloned lysostaphin gene |
Publications (3)
Publication Number | Publication Date |
---|---|
DK659287A true DK659287A (en) | 1987-12-15 |
DK659287D0 DK659287D0 (en) | 1987-12-15 |
DK174941B1 DK174941B1 (en) | 2004-03-08 |
Family
ID=26710985
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK198706592A DK174941B1 (en) | 1986-04-16 | 1987-12-15 | DNA fragment encoding lysostaphin, recombinant plasmids containing it, transformed microorganisms as well as expression of the cloned lysostaphin |
Country Status (15)
Country | Link |
---|---|
US (1) | US4931390A (en) |
EP (1) | EP0299978B1 (en) |
JP (1) | JP2963695B2 (en) |
AT (1) | ATE92524T1 (en) |
AU (1) | AU616379B2 (en) |
CA (1) | CA1297051C (en) |
DE (1) | DE3786918T2 (en) |
DK (1) | DK174941B1 (en) |
FI (1) | FI96322C (en) |
HU (1) | HU205386B (en) |
IE (1) | IE60769B1 (en) |
IL (1) | IL82260A (en) |
NZ (1) | NZ219998A (en) |
PT (1) | PT84705B (en) |
WO (1) | WO1987006264A1 (en) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5260212A (en) * | 1987-02-09 | 1993-11-09 | Quest International Flavors & Food Ingredients Co., Division Of Indopco | Cloned gene encoding for bacteriocin from Pediococcus acidilactici |
US5858962A (en) * | 1987-05-11 | 1999-01-12 | Ambi Inc. | Composition for treating mastitis and other staphylococcal infections |
US5011772A (en) * | 1988-02-05 | 1991-04-30 | Public Health Research Institute Of The City Of N.Y. | High yield protein production system |
WO1989010976A1 (en) * | 1988-05-05 | 1989-11-16 | The Public Health Research Institute Of The City O | Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products |
CA2009336A1 (en) * | 1989-07-03 | 1991-01-03 | Peter A. Vandenbergh | Cloned gene encoding for bacteriocin from pediococcus acidilactici |
DE4425645A1 (en) * | 1994-07-20 | 1996-02-22 | Mueller Karl & Co Kg | New lysostaphin gene with deletion in pro-segment repeat region |
JP4236699B2 (en) * | 1995-06-23 | 2009-03-11 | アンビィー インコーポレイテッド | Control of antibiotic-resistant Gram-positive bacteria and methods of treatment for infection |
WO1997008553A1 (en) * | 1995-08-22 | 1997-03-06 | The Regents Of The University Of California | Targeting of proteins to the cell wall of gram-positive bacteria |
US5910479A (en) * | 1996-10-18 | 1999-06-08 | Ambi Inc. | Method for the treatment of Streptococcus pneumoniae infection |
US6028051A (en) * | 1997-07-23 | 2000-02-22 | Ambi Inc. | Method for the treatment of staphylococcal disease |
US7091332B1 (en) | 1998-06-22 | 2006-08-15 | University Of Vermont | Treatment of staphylococcus infections |
US6875903B2 (en) * | 1998-06-22 | 2005-04-05 | University Of Vermont | Treatment of Staphylococcus infections |
WO1999067381A1 (en) * | 1998-06-22 | 1999-12-29 | University Of Vermont And State Agricultural College | Treatment of staphylococcus infections |
JP4416866B2 (en) * | 1998-07-30 | 2010-02-17 | オリエンタル酵母工業株式会社 | Liquid reagent for calcium measurement |
US6569830B1 (en) * | 1999-03-05 | 2003-05-27 | Ambi, Inc. | Compositions and methods for treatment of staphylococcal infection while suppressing formation of antibiotic-resistant strains |
AU784354B2 (en) * | 1999-10-19 | 2006-03-16 | Bharat Biotech International, Ltd. | Expression of recombinant mature lysostaphin |
JP4348194B2 (en) * | 2001-12-21 | 2009-10-21 | バイオシネクサス インコーポレーテッド | A truncated lysostaphin molecule with enhanced staphylococcal lytic activity |
BR0308285A (en) * | 2002-03-26 | 2005-04-12 | Biosynexus Inc | Bacterial biofilm disruption by enzymes |
BR0308710A (en) * | 2002-03-26 | 2007-01-09 | Biosynexus Inc | antimicrobial polymer conjugates |
US20100221237A1 (en) * | 2003-03-26 | 2010-09-02 | Biosynexus Incorporated | Enzyme disruption of bacterial biofilms |
KR101004338B1 (en) | 2003-03-28 | 2010-12-28 | 미쓰이 가가쿠 가부시키가이샤 | Propylene?1?butene copolymer, polypropylene composition, use thereof, and polypropylene composite film |
CN100485031C (en) * | 2004-09-02 | 2009-05-06 | 上海高科联合生物技术研发有限公司 | Lysostaphin industrial purifying process |
WO2006110776A2 (en) | 2005-04-12 | 2006-10-19 | Nektar Therapeutics Al, Corporation | Polyethylene glycol cojugates of antimicrobial agents |
CN100475965C (en) | 2005-07-22 | 2009-04-08 | 上海高科联合生物技术研发有限公司 | Method for E, coli to express lysostaphin in high efficiency via external secretion |
US20080095756A1 (en) * | 2006-09-05 | 2008-04-24 | Biosynexus Incorporated | Compositions Comprising Lysostaphin Variants And Methods Of Using The Same |
CN101849019A (en) * | 2007-07-09 | 2010-09-29 | 微敏医疗技术有限公司 | Detection of micro-organisms based on their NAD-dependent DNA ligase activity |
WO2009114520A2 (en) * | 2008-03-10 | 2009-09-17 | Pharmain Corporation | Compositions for treatment with metallopeptidases, methods of making and using the same |
WO2012050787A1 (en) | 2010-09-29 | 2012-04-19 | Ibis Biosciences, Inc. | Fungal nucleic acid extraction |
CN113717964A (en) * | 2013-08-06 | 2021-11-30 | 达特茅斯学院理事会 | Non-glycosylated lysostaphin variant protein |
WO2015100448A2 (en) | 2013-12-28 | 2015-07-02 | Longhorn Vaccines And Diagnostics, Llc | Multimodal antimicrobial therapy |
JP7059003B2 (en) | 2014-05-14 | 2022-04-25 | トラスティーズ・オブ・ダートマス・カレッジ | Deimmunized lysostaphin and how to use |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3278378A (en) * | 1962-04-19 | 1966-10-11 | Schindler Charles Alvin | Staphylococcus-derived antibiotic |
US4467036A (en) * | 1981-11-12 | 1984-08-21 | The Board Of Regents Of The University Of Washington | Bacillus thuringiensis crystal protein in Escherichia coli |
US4617266A (en) * | 1983-04-28 | 1986-10-14 | Genex Corporation | Production of Protein A |
-
1987
- 1987-04-10 US US07/034,464 patent/US4931390A/en not_active Expired - Lifetime
- 1987-04-14 CA CA000534626A patent/CA1297051C/en not_active Expired - Lifetime
- 1987-04-15 DE DE87903128T patent/DE3786918T2/en not_active Expired - Fee Related
- 1987-04-15 JP JP62502621A patent/JP2963695B2/en not_active Expired - Lifetime
- 1987-04-15 EP EP87903128A patent/EP0299978B1/en not_active Expired - Lifetime
- 1987-04-15 IL IL8782260A patent/IL82260A/en not_active IP Right Cessation
- 1987-04-15 AU AU73021/87A patent/AU616379B2/en not_active Ceased
- 1987-04-15 WO PCT/US1987/000873 patent/WO1987006264A1/en active IP Right Grant
- 1987-04-15 IE IE98087A patent/IE60769B1/en not_active IP Right Cessation
- 1987-04-15 AT AT87903128T patent/ATE92524T1/en not_active IP Right Cessation
- 1987-04-15 HU HU872384A patent/HU205386B/en not_active IP Right Cessation
- 1987-04-15 NZ NZ219998A patent/NZ219998A/en unknown
- 1987-04-16 PT PT84705A patent/PT84705B/en unknown
- 1987-12-15 DK DK198706592A patent/DK174941B1/en not_active IP Right Cessation
-
1988
- 1988-10-12 FI FI884691A patent/FI96322C/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
WO1987006264A1 (en) | 1987-10-22 |
DK174941B1 (en) | 2004-03-08 |
EP0299978B1 (en) | 1993-08-04 |
NZ219998A (en) | 1991-05-28 |
JP2963695B2 (en) | 1999-10-18 |
IL82260A0 (en) | 1987-10-30 |
DE3786918T2 (en) | 1993-11-11 |
HUT50874A (en) | 1990-03-28 |
PT84705A (en) | 1987-05-01 |
FI884691A0 (en) | 1988-10-12 |
JPH01503036A (en) | 1989-10-19 |
FI96322C (en) | 1996-06-10 |
IE60769B1 (en) | 1994-08-10 |
AU7302187A (en) | 1987-11-09 |
CA1297051C (en) | 1992-03-10 |
ATE92524T1 (en) | 1993-08-15 |
IL82260A (en) | 1992-08-18 |
US4931390A (en) | 1990-06-05 |
IE870980L (en) | 1987-10-16 |
DK659287D0 (en) | 1987-12-15 |
FI96322B (en) | 1996-02-29 |
PT84705B (en) | 1989-11-30 |
FI884691A (en) | 1988-10-12 |
DE3786918D1 (en) | 1993-09-09 |
AU616379B2 (en) | 1991-10-31 |
EP0299978A1 (en) | 1989-01-25 |
HU205386B (en) | 1992-04-28 |
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