DK355486D0 - FUSION PROTEINS, THEIR PREPARATION AND USE - Google Patents

FUSION PROTEINS, THEIR PREPARATION AND USE

Info

Publication number
DK355486D0
DK355486D0 DK355486A DK355486A DK355486D0 DK 355486 D0 DK355486 D0 DK 355486D0 DK 355486 A DK355486 A DK 355486A DK 355486 A DK355486 A DK 355486A DK 355486 D0 DK355486 D0 DK 355486D0
Authority
DK
Denmark
Prior art keywords
sequence
amino acids
genetically codable
genetically
fusion protein
Prior art date
Application number
DK355486A
Other languages
Danish (da)
Other versions
DK355486A (en
DK172618B1 (en
Inventor
Paul Habermann
Siegfried Stengelin
Friedrich Wengenmayer
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of DK355486D0 publication Critical patent/DK355486D0/en
Publication of DK355486A publication Critical patent/DK355486A/en
Application granted granted Critical
Publication of DK172618B1 publication Critical patent/DK172618B1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/62Insulins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/815Protease inhibitors from leeches, e.g. hirudin, eglin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/33Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1. Claims for the Contracting States : BE, CH, DE, FR, GB, IT, LI, LU, NL, SE A fusion protein of the general formula Met - Xn - D' - Y - Z in which n is zero or 1, X is a sequence of 1 to 12 genetically codable amino acids, D' is a sequence of about 70 amino acids in the region of the sequence of amino acids 23 - 93 of the D-peptide in the trp operon of E. coli, Y denotes a sequence of one or more genetically codable amino acids which permits the following amino acid sequence Z to be cleaved off, and Z is a sequence of genetically codable amino acids. 1. Claims for the Contracting State : AT A process for the preparation of a fusion protein of the general formula (1) Met - Xn - D' - Y - Z in which n is zero or 1, X is a sequence of 1 to 12 genetically codable amino acids, D' is a sequence of about 70 amino acids in the region of the sequence of amino acids 23 - 93 of the D-peptide in the trp operon of E. coli, Y denotes a sequence of one or more genetically codable amino acids which permits the following amino acid sequence Z to be cleaved off, and Z is a sequence of genetically codable amino acids, characterized by expressing a gene structure which codes for these fusion proteins in a host cell, and separating the fusion protein.
DK198603554A 1985-07-27 1986-07-25 Fusion proteins, their preparation and use DK172618B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3526995 1985-07-27
DE19853526995 DE3526995A1 (en) 1985-07-27 1985-07-27 FUSION PROTEINS, METHOD FOR THEIR PRODUCTION AND THEIR USE

Publications (3)

Publication Number Publication Date
DK355486D0 true DK355486D0 (en) 1986-07-25
DK355486A DK355486A (en) 1987-01-28
DK172618B1 DK172618B1 (en) 1999-03-01

Family

ID=6276985

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198603554A DK172618B1 (en) 1985-07-27 1986-07-25 Fusion proteins, their preparation and use

Country Status (18)

Country Link
EP (1) EP0211299B1 (en)
JP (1) JPH07113040B2 (en)
KR (1) KR950000299B1 (en)
AT (1) ATE50596T1 (en)
AU (1) AU595221B2 (en)
CA (1) CA1336329C (en)
DE (2) DE3526995A1 (en)
DK (1) DK172618B1 (en)
ES (1) ES2000278A6 (en)
FI (1) FI86887C (en)
GR (1) GR861957B (en)
HU (1) HU197356B (en)
IE (1) IE59127B1 (en)
IL (1) IL79522A (en)
NO (1) NO175640C (en)
NZ (1) NZ216967A (en)
PT (1) PT83065B (en)
ZA (1) ZA865556B (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3738541A1 (en) * 1987-11-13 1989-05-24 Hoechst Ag METHOD FOR INSULATING AND CLEANING HIRUDINE
DE3541856A1 (en) * 1985-11-27 1987-06-04 Hoechst Ag EUKARYOTIC FUSION PROTEINS, THEIR PRODUCTION AND USE, AND MEANS FOR CARRYING OUT THE PROCESS
EP0237338B1 (en) * 1986-03-12 1992-09-23 IMCERA Group Inc. Hybrid expression vectors, their construction and uses
EP0371041A1 (en) * 1987-06-24 1990-06-06 Novo Nordisk A/S A process for preparing a protein or polypeptide, a dna sequence coding for the polypeptide, a microorganism containing the dna sequence as well as the polypeptide and its use as a pharmaceutical preparation
US5179196A (en) * 1989-05-04 1993-01-12 Sri International Purification of proteins employing ctap-iii fusions
US5227293A (en) * 1989-08-29 1993-07-13 The General Hospital Corporation Fusion proteins, their preparation and use
US5358857A (en) * 1989-08-29 1994-10-25 The General Hospital Corp. Method of preparing fusion proteins
IL95495A (en) * 1989-08-29 1996-10-16 Hoechst Ag Fusion proteins their preparation and use
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
DE3942580A1 (en) * 1989-12-22 1991-06-27 Basf Ag METHOD FOR PRODUCING HIRUDINE
DE59711533D1 (en) 1996-07-26 2004-05-27 Aventis Pharma Gmbh Insulin derivatives with increased zinc binding
DE19726167B4 (en) 1997-06-20 2008-01-24 Sanofi-Aventis Deutschland Gmbh Insulin, process for its preparation and pharmaceutical preparation containing it
DE19825447A1 (en) 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh New insulin analogues with increased zinc formation
DE10114178A1 (en) 2001-03-23 2002-10-10 Aventis Pharma Gmbh Zinc-free and low-zinc insulin preparations with improved stability
DE10227232A1 (en) 2002-06-18 2004-01-15 Aventis Pharma Deutschland Gmbh Sour insulin preparations with improved stability
WO2009066320A2 (en) 2007-09-21 2009-05-28 Cadila Healthcare Limited Fusion protein systems suitable for high expression of peptides
EP3228320B1 (en) 2008-10-17 2019-12-18 Sanofi-Aventis Deutschland GmbH Combination of an insulin and a glp-1 agonist
AR080669A1 (en) 2009-11-13 2012-05-02 Sanofi Aventis Deutschland PHARMACEUTICAL COMPOSITION INCLUDING A GLP-1 AGONIST, AN INSULIN AND METIONIN
UY33025A (en) 2009-11-13 2011-06-30 Sanofi Aventis Deustschland Gmbh PHARMACEUTICAL COMPOSITION INCLUDING A GLP-1 METIONINE AGONIST
MX339614B (en) 2010-08-30 2016-06-02 Sanofi - Aventis Deutschland GmbH Use of ave0010 for the manufacture of a medicament for the treatment of diabetes mellitus type 2.
US9821032B2 (en) 2011-05-13 2017-11-21 Sanofi-Aventis Deutschland Gmbh Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
RU2650616C2 (en) 2011-08-29 2018-04-16 Санофи-Авентис Дойчланд Гмбх Pharmaceutical combination for use in glycemic control in patients with type 2 diabetes mellitus
TWI559929B (en) 2011-09-01 2016-12-01 Sanofi Aventis Deutschland Pharmaceutical composition for use in the treatment of a neurodegenerative disease
CN107206058A (en) 2014-12-12 2017-09-26 赛诺菲-安万特德国有限公司 Insulin glargine/lixisenatide fixed ratio preparaton
TWI748945B (en) 2015-03-13 2021-12-11 德商賽諾菲阿凡提斯德意志有限公司 Treatment type 2 diabetes mellitus patients
TW201705975A (en) 2015-03-18 2017-02-16 賽諾菲阿凡提斯德意志有限公司 Treatment of type 2 diabetes mellitus patients
KR20200080748A (en) 2018-12-27 2020-07-07 주식회사 폴루스 A Method for Purifying Proinsulin Using Anion Exchange Chromatography
KR20200080747A (en) 2018-12-27 2020-07-07 주식회사 폴루스 An Enzymatic Conversion Composition for Producing Insulin from Proinsulin and a Method for Producing Insulin from Proinsulin Using the Same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA802992B (en) * 1979-06-01 1981-10-28 Univ California Human pre-growth hormone
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
JPS62502586A (en) * 1985-03-18 1987-10-08 ジ−ン ラブズ,インコ−ポレイテツド Hybrid gene cassette vector
GB8507666D0 (en) * 1985-03-25 1985-05-01 Wellcome Found Epidermal growth factor production
GR860915B (en) * 1985-04-08 1986-07-11 Genetic Systems Corp Expression and diagnostic use of gag encoded peptides which are immunologically reactive with antibodies to lav

Also Published As

Publication number Publication date
FI863041A (en) 1987-01-28
DK355486A (en) 1987-01-28
KR950000299B1 (en) 1995-01-13
EP0211299A3 (en) 1988-06-01
DE3669175D1 (en) 1990-04-05
DE3526995A1 (en) 1987-02-05
FI863041A0 (en) 1986-07-24
JPS6229600A (en) 1987-02-07
DK172618B1 (en) 1999-03-01
FI86887B (en) 1992-07-15
JPH07113040B2 (en) 1995-12-06
NO863000D0 (en) 1986-07-25
PT83065A (en) 1986-08-01
IE861977L (en) 1987-01-27
ES2000278A6 (en) 1988-02-01
EP0211299B1 (en) 1990-02-28
IE59127B1 (en) 1994-01-12
IL79522A (en) 1991-08-16
NZ216967A (en) 1988-08-30
EP0211299A2 (en) 1987-02-25
HUT43629A (en) 1987-11-30
NO175640B (en) 1994-08-01
IL79522A0 (en) 1986-10-31
GR861957B (en) 1986-11-25
PT83065B (en) 1989-02-28
FI86887C (en) 1992-10-26
KR870001312A (en) 1987-03-13
NO863000L (en) 1987-01-28
ZA865556B (en) 1987-03-25
CA1336329C (en) 1995-07-18
ATE50596T1 (en) 1990-03-15
AU6056586A (en) 1987-01-29
AU595221B2 (en) 1990-03-29
HU197356B (en) 1989-03-28
NO175640C (en) 1994-11-09

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Legal Events

Date Code Title Description
B1 Patent granted (law 1993)
PUP Patent expired