DK348288A - PROCEDURE FOR PREPARING A DESIRED PROTEIN - Google Patents

PROCEDURE FOR PREPARING A DESIRED PROTEIN

Info

Publication number
DK348288A
DK348288A DK348288A DK348288A DK348288A DK 348288 A DK348288 A DK 348288A DK 348288 A DK348288 A DK 348288A DK 348288 A DK348288 A DK 348288A DK 348288 A DK348288 A DK 348288A
Authority
DK
Denmark
Prior art keywords
lys
arg
pro
different
amino acids
Prior art date
Application number
DK348288A
Other languages
Danish (da)
Other versions
DK166687B1 (en
DK348288D0 (en
Inventor
Henrik Dalboege
John Pedersen
Thorkild Christensen
Joerli Winnie Ringsted
Original Assignee
Nordisk Gentofte
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nordisk Gentofte filed Critical Nordisk Gentofte
Priority to DK348288A priority Critical patent/DK166687B1/en
Publication of DK348288A publication Critical patent/DK348288A/en
Publication of DK348288D0 publication Critical patent/DK348288D0/en
Application granted granted Critical
Publication of DK166687B1 publication Critical patent/DK166687B1/en

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A desired protein of the formula: A-B-C-P where a) A is Lys or Arg, and B and C are arbitrary amino acids, or b) A is an arbitrary amino acid which is different from Pro, Lys and Arg, and B and/or C is Pro, is prepared from a biosynthetically formed amino acid- extended protein of the formula: X-A-B-C-P where A, B, C and P have the abovementioned meaning, and X is an amino acid sequence having an even number of amino acids, of which the first, calculated from the N- terminal end, is different from Lys and Arg, all the other odd amino acids are different from Pro, Lys and Arg, and all the even amino acids are different from Pro, by reacting with the enzyme dipeptidal aminopeptidase (DAP I). The desired protein is obtained in a pure state. Thus, hGH which does not contain any met-hGH can, for example, be prepared in the process.
DK348288A 1988-06-24 1988-06-24 Human growth hormone prodn. - by reacting amino terminal extended hormone with di:peptidyl aminopeptidase DK166687B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK348288A DK166687B1 (en) 1988-06-24 1988-06-24 Human growth hormone prodn. - by reacting amino terminal extended hormone with di:peptidyl aminopeptidase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK348288 1988-06-24
DK348288A DK166687B1 (en) 1988-06-24 1988-06-24 Human growth hormone prodn. - by reacting amino terminal extended hormone with di:peptidyl aminopeptidase

Publications (3)

Publication Number Publication Date
DK348288A true DK348288A (en) 1988-06-24
DK348288D0 DK348288D0 (en) 1988-06-24
DK166687B1 DK166687B1 (en) 1993-06-28

Family

ID=8123406

Family Applications (1)

Application Number Title Priority Date Filing Date
DK348288A DK166687B1 (en) 1988-06-24 1988-06-24 Human growth hormone prodn. - by reacting amino terminal extended hormone with di:peptidyl aminopeptidase

Country Status (1)

Country Link
DK (1) DK166687B1 (en)

Also Published As

Publication number Publication date
DK166687B1 (en) 1993-06-28
DK348288D0 (en) 1988-06-24

Similar Documents

Publication Publication Date Title
KR870004056A (en) Selective chemical removal of protein amino-termination residues
EP0307592A3 (en) Analogues of the pancreatic bovine trypsin inhibitor, their production and use
DE69020794D1 (en) Enzymatic removal of an amino terminal protein sequence.
DE3588249D1 (en) Method of producing human growth hormones
RO80806A (en) PROCEDURE FOR ENZYMATIC OBTAINING PEPTIDES
FI880017A0 (en) Method for preparing pancreatic trypsin inhibitor variants and DNA, vector and host cell used in the method
DK390689D0 (en) METHOD FOR PREPARING DES-B30 INSULINES AND DES-B30 INSULIN DERIVATIVES
ATE40556T1 (en) METHOD OF SYNTHESIS OF PEPTIDES.
DK348288A (en) PROCEDURE FOR PREPARING A DESIRED PROTEIN
EP0330700A4 (en) Human prourokinase-like polypeptide.
ATE144551T1 (en) METHOD OF PRODUCING GLUCAGON
SE8702520D0 (en) MAMMAL INTESTINE HORMONE PRECURSOR
KR890008166A (en) Selective degradation of fusion proteins
ATE224403T1 (en) USE OF CASEIN FRAGMENTS AS GROWTH PROMOTERS
ATE101616T1 (en) PROCESS FOR THE PREPARATION OF DIPEPTIDES.
EP0326799A3 (en) Enzymatic process for synthesizing peptides
DK56995A (en) Recombinant lymphotoxin variants
JPH01190700A (en) Novel peptide

Legal Events

Date Code Title Description
PUP Patent expired