DK283A - PROCEDURE FOR STABILIZING Aqueous SOLUTIONS OF CHOLESTERINE ANESTHESIS FROM PSEUDOMONADS - Google Patents
PROCEDURE FOR STABILIZING Aqueous SOLUTIONS OF CHOLESTERINE ANESTHESIS FROM PSEUDOMONADS Download PDFInfo
- Publication number
- DK283A DK283A DK283A DK283A DK283A DK 283 A DK283 A DK 283A DK 283 A DK283 A DK 283A DK 283 A DK283 A DK 283A DK 283 A DK283 A DK 283A
- Authority
- DK
- Denmark
- Prior art keywords
- anesthesis
- cholesterine
- pseudomonads
- procedure
- aqueous solutions
- Prior art date
Links
- 239000007864 aqueous solution Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title 1
- 230000000087 stabilizing effect Effects 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 abstract 1
- 241000589516 Pseudomonas Species 0.000 abstract 1
- 108010055297 Sterol Esterase Proteins 0.000 abstract 1
- 102000000019 Sterol Esterase Human genes 0.000 abstract 1
- 229910001425 magnesium ion Inorganic materials 0.000 abstract 1
- 230000006641 stabilisation Effects 0.000 abstract 1
- 238000011105 stabilization Methods 0.000 abstract 1
- 239000004094 surface-active agent Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Steroid Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Detergent Compositions (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fats And Perfumes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention provides a process for the stabilization of an aqueous solution of cholesterol esterase from Pseudomonas, especially in the presence of a surface-active agent, wherein the enzyme is dissolved in a phosphate-free buffer which contains 10 to 200 mMol/liter of magnesium ions.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823200274 DE3200274A1 (en) | 1982-01-07 | 1982-01-07 | METHOD FOR STABILIZING AQUEOUS SOLUTIONS OF CHOLESTERINESTERASE FROM PSEUDOMONADES |
| DE3200274 | 1982-01-07 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| DK283D0 DK283D0 (en) | 1983-01-03 |
| DK283A true DK283A (en) | 1983-07-08 |
| DK163527B DK163527B (en) | 1992-03-09 |
| DK163527C DK163527C (en) | 1992-08-03 |
Family
ID=6152717
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK000283A DK163527C (en) | 1982-01-07 | 1983-01-03 | PROCEDURE FOR STABILIZING Aqueous SOLUTIONS OF CHOLESTERINE INSTERASE FROM MICROORGANISMS OF THE GENE PSEUDOMONAS |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US4476223A (en) |
| EP (1) | EP0084684B1 (en) |
| JP (1) | JPS58175488A (en) |
| AT (1) | ATE12659T1 (en) |
| AU (1) | AU553593B2 (en) |
| CA (1) | CA1198698A (en) |
| DD (1) | DD208824A5 (en) |
| DE (2) | DE3200274A1 (en) |
| DK (1) | DK163527C (en) |
| ES (1) | ES8402347A1 (en) |
| FI (1) | FI77262C (en) |
| ZA (1) | ZA8367B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6030680A (en) * | 1983-07-30 | 1985-02-16 | Amano Pharmaceut Co Ltd | Method for imparting esterase activity to lipase |
| DE3447390A1 (en) * | 1984-12-24 | 1986-07-03 | Boehringer Mannheim Gmbh, 6800 Mannheim | METHOD FOR OBTAINING CHOLESTERINESTERASE |
| EP0218083A1 (en) * | 1985-09-03 | 1987-04-15 | Abbott Laboratories | Stabilized cholesterol reagent and method for determining total cholesterol using the reagent |
| DK145090D0 (en) * | 1990-06-14 | 1990-06-14 | Novo Nordisk As | CELLULASE PREPARATIONS AND APPLICATIONS THEREOF |
| US5460944A (en) * | 1991-10-28 | 1995-10-24 | Boehringer Mannheim Gmbh | Storable protein solution |
| DK99492D0 (en) * | 1992-08-07 | 1992-08-07 | Novo Nordisk As | NEW ENZYM |
| EP3722418A1 (en) * | 2019-04-08 | 2020-10-14 | AB Enzymes Oy | Solution stable enzyme composition |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR891187A (en) * | 1942-09-25 | 1944-02-29 | Preservation process for rennet | |
| US3884764A (en) * | 1974-03-25 | 1975-05-20 | Eastman Kodak Co | Method and composition for blood serum cholesterol analysis |
| DE2911284C2 (en) * | 1979-03-22 | 1982-01-28 | Boehringer Mannheim Gmbh, 6800 Mannheim | Method and reagent for activating cholesterol esterase |
| DE2933648A1 (en) * | 1979-08-20 | 1981-03-26 | Boehringer Mannheim Gmbh, 68305 Mannheim | METHOD FOR OBTAINING CHOLESTERINESTERASE |
| DE2933646A1 (en) * | 1979-08-20 | 1981-03-26 | Boehringer Mannheim Gmbh, 68305 Mannheim | METHOD FOR OBTAINING CHOLESTERINESTERASE |
| US4409326A (en) * | 1980-07-10 | 1983-10-11 | Modrovich Ivan Endre | Stabilized enzymatic solutions and method for determining total cholesterol in human serum |
-
1982
- 1982-01-07 DE DE19823200274 patent/DE3200274A1/en not_active Withdrawn
- 1982-12-15 CA CA000417722A patent/CA1198698A/en not_active Expired
- 1982-12-20 AU AU91668/82A patent/AU553593B2/en not_active Ceased
- 1982-12-23 US US06/452,690 patent/US4476223A/en not_active Expired - Lifetime
- 1982-12-30 ES ES518694A patent/ES8402347A1/en not_active Expired
- 1982-12-31 DE DE8282112129T patent/DE3263043D1/en not_active Expired
- 1982-12-31 AT AT82112129T patent/ATE12659T1/en not_active IP Right Cessation
- 1982-12-31 EP EP82112129A patent/EP0084684B1/en not_active Expired
-
1983
- 1983-01-03 DK DK000283A patent/DK163527C/en not_active IP Right Cessation
- 1983-01-05 DD DD83247040A patent/DD208824A5/en unknown
- 1983-01-06 FI FI830036A patent/FI77262C/en not_active IP Right Cessation
- 1983-01-06 ZA ZA8367A patent/ZA8367B/en unknown
- 1983-01-07 JP JP58000568A patent/JPS58175488A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| ZA8367B (en) | 1983-10-26 |
| ATE12659T1 (en) | 1985-04-15 |
| AU9166882A (en) | 1983-07-14 |
| ES518694A0 (en) | 1984-02-16 |
| DK163527C (en) | 1992-08-03 |
| EP0084684B1 (en) | 1985-04-10 |
| EP0084684A1 (en) | 1983-08-03 |
| AU553593B2 (en) | 1986-07-24 |
| DE3263043D1 (en) | 1985-05-15 |
| FI77262B (en) | 1988-10-31 |
| JPS58175488A (en) | 1983-10-14 |
| DK163527B (en) | 1992-03-09 |
| CA1198698A (en) | 1985-12-31 |
| ES8402347A1 (en) | 1984-02-16 |
| FI830036L (en) | 1983-07-08 |
| DK283D0 (en) | 1983-01-03 |
| DD208824A5 (en) | 1984-04-11 |
| FI830036A0 (en) | 1983-01-06 |
| JPS6121076B2 (en) | 1986-05-24 |
| US4476223A (en) | 1984-10-09 |
| DE3200274A1 (en) | 1983-07-14 |
| FI77262C (en) | 1989-02-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PBP | Patent lapsed |