DK2281631T3 - Modular microfluidic sample preparation system and method of mixing and delivering a sample fluid. - Google Patents

Modular microfluidic sample preparation system and method of mixing and delivering a sample fluid. Download PDF

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Publication number
DK2281631T3
DK2281631T3 DK09167507.4T DK09167507T DK2281631T3 DK 2281631 T3 DK2281631 T3 DK 2281631T3 DK 09167507 T DK09167507 T DK 09167507T DK 2281631 T3 DK2281631 T3 DK 2281631T3
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preparation module
preparation
sample
module
fluid
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DK09167507.4T
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English (en)
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Reza Nikbakht
Peter Warthoe
Jens Mikkelsen
Iben Schildt Sørensen
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Atonomics As
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B01L3/5635—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors connecting two containers face to face, e.g. comprising a filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/028—Modular arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/021—Identification, e.g. bar codes
    • B01L2300/022—Transponder chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (12)

1. Modulært mikrofluidprøvepræpareringssystem (1), hvor systemet omfatter: - et første præpareringsmodul (2), som er anbragt i et første plan, - idet det første præpareringsmodul (2) omfatter en første overflade (24) og en modsat anden overflade (25) og er afgrænset af første laterale flader (7), - idet det første præpareringsmodul (2) yderligere omfatter en udgang (38), som er anbragt ved den anden overflade (25) af det første præpareringsmodul (2), og en indgang (4), hvor indgangen (4) og udgangen (38) er forbundet via et første mikrofluidkanalsystem (26), - et andet præpareringsmodul (3), som er anbragt i et andet plan, der i det væsentlige er parallelt med det første plan, - idet det andet præpareringsmodul (3) omfatter en første overflade og en modsat anden overflade og er afgrænset af anden laterale flader (8), - idet det andet præpareringsmodul (3) yderligere omfatter et indtag (10), som er anbragt ved den første overflade af det andet præpareringsmodul (3), hvor indtaget (10) omfatter et blodsepareringsfilter (40), idet indtaget (10) er forbundet med et andet mikrofluidkanalsystem (50), og hvor - det første præpareringsmodul (2) er forbundet med det andet præpareringsmodul (3), således at den anden overflade (25) af det første præpareringsmodul (2) vender imod den første overflade af det andet præpareringsmodul (3), og således at udgangen (38) i det første præpareringsmodul (2) vender imod indtaget (10) i det andet præpareringsmodul (2), og således at udgangen (38) i det første præpareringsmodul (2) er anbragt med en afstand (d) fra indtaget (10) i det andet præpareringsmodul (3).
2. Modulært mikrofluidprøvepræpareringssystem (1) ifølge krav 1, hvor det første præpareringsmodul (2) og det andet præpareringsmodul (3) omfatter forbindelsesmidler (9, 39) til udløseligt at forbinde det første præpareringsmodul (2) med det andet præpareringsmodul (3).
3. Modulært mikrofluidprøvepræpareringssystem (1) ifølge krav 1 eller 2, hvor afstanden (d) mellem udgangen (38) i det første præpareringsmodul (2) og indtaget (10) i det andet præpareringsmodul (3) er 0,05-1 mm eller 0,1-0,9 mm eller 0,15-0,8 mm.
4. Modulært mikrofluidprøvepræpareringssystem (1) ifølge et hvilket som helst af de foregående krav, hvor det første præpareringsmodul (2) omfatter et mikrofluidblandingsafsnit (29), der er i fluidforbindelse med det første mi krofluid kanalsystem (26).
5. Modulært mikrofluidprøvepræpareringssystem (1) ifølge krav 4, hvor det første præpareringsmodul (2) omfatter et tilsætningsstofreservoir (34), der er i fluidforbindelse med blandingsafsnittet (29).
6. Modulært mikrofluidprøvepræpareringssystem (1) ifølge et hvilket som helst af de foregående krav, hvor det første præpareringsmodul (2) omfatter et indgangsreservoir (6), der er i fluidforbindelse med indgangen (4) og det første mi krofluid kanalsystem (26) i det første præpareringsmodul (2).
7. Modulært mikrofluidprøvepræpareringssystem (1) ifølge et hvilket som helst af de foregående krav, hvor det første præpareringsmodul (2) omfatter en transparent kanal (6) (synlig for slutbrugeren).
8. Modulært mikrofluidprøvepræpareringssystem (1) ifølge et hvilket som helst af de foregående krav, hvor det første præpareringsmodul (2) endvidere omfatter et pumpemiddel (31).
9. Anvendelse af et modulært mikrofluidprøvepræpareringssystem (1) ifølge et hvilket som helst af kravene 1-8 til præparering af en blodprøve.
10. Blodanalyseapparat, hvor der anvendes et modulært mikrofluid-prøvepræpareringssystem (1) ifølge et hvilket som helst af kravene 1-8.
11. Blodanalyseapparat ifølge krav 10, der omfatter et modulært mikrofluidprøvepræpareringssystem (1) ifølge krav 8, hvor pumpemidlet (31) i det første præpareringsmodul (2) aktiveres af blodanalyseapparatet.
12. Fremgangsmåde til blanding et prøvefluid (33) og et tilsætningsstof i et første præpareringsmodul (2) og afgivelse af det blandede prøvefluid (37) til et andet præpareringsmodul (3) under anvendelse af et modulært mikrofluidprøvepræpareringssystem (1), hvilken fremgangsmåde omfatter følgende trin: - forbindelse af et første præpareringsmodul (2), der omfatter et første mikrofluidkanalsystem (26), med et andet præpareringsmodul (3), idet det andet præpareringsmodul (3) omfatter et andet mikrofluidkanalsystem (50), - tilførsel af et prøvefluid (33) til et indgangsreservoir (6) gennem en indgang (4) i det første præpareringsmodul (2), - lukning af indgangen (4) i det første præpareringsmodul (2) ved hjælp af lukkemidler (5), - fremdrivning af prøvefluidet (33) fra indgangsreservoiret ind i et blandingsafsnit (26) i det første præpareringsmodul (2) ved hjælp af lufttryk, - tilførsel af et tilsætningsstof fra et tilsætningsstofreservoir (34) til blandingsafsnittet (26) i det første præpareringsmodul (2), - blanding af tilsætningsstoffet og prøvefluidet (33) ved ændring af det tryk, som luften påfører fluidet i blandingsafsnittet (26), hvorved der dannes et blandet prøvefluid (37), - tilvejebringelse af et hydrostatisk tryk på det blandede prøvefluid (37), idet det hydrostatiske tryk, der tilvejebringes i det første præpareringsmodul (2) ved hjælp af pumpemidlerne (31), dermed fremdriver det blandede prøvefluid (37) gennem udgangen (38) i det første præpareringsmodul (2), som er anbragt med en afstand (d) fra indtaget (10) i det andet præpareringsmodul (3), til indtaget (10) i det andet præpareringsmodul (3), hvor indtaget (10) omfatter et blodsepareringsfilter (40), og - mindst delvis overførsel af det blandede prøvefluid (37) fra indtaget til det andet mikrofluidkanalsystem (50), som er omfattet i det andet præpareringsmodul (3), ved hjælp af kapillærkræfter.
DK09167507.4T 2009-08-07 2009-08-07 Modular microfluidic sample preparation system and method of mixing and delivering a sample fluid. DK2281631T3 (da)

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EP09167507.4A EP2281631B1 (en) 2009-08-07 2009-08-07 Modular microfluidic sample preparation system and method of mixing and delivering a sample fluid.

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WO2013071301A1 (en) * 2011-11-10 2013-05-16 The Administrators Of The Tulane Educational Fund Paper based diagnostic test
AU2012327218C1 (en) 2011-11-30 2015-12-24 Wellstat Diagnostics, Llc. Filtration Module
US9075042B2 (en) 2012-05-15 2015-07-07 Wellstat Diagnostics, Llc Diagnostic systems and cartridges
US9213043B2 (en) 2012-05-15 2015-12-15 Wellstat Diagnostics, Llc Clinical diagnostic system including instrument and cartridge
US9625465B2 (en) 2012-05-15 2017-04-18 Defined Diagnostics, Llc Clinical diagnostic systems
US10434508B2 (en) 2014-07-03 2019-10-08 Abionic Sa Capsule for rapid molecular quantification of a fluid sample such as whole blood
US11085931B2 (en) 2015-01-09 2021-08-10 W. Health L.P. Universal assay for determining the quantity of TNFα inhibitory drugs and their corresponding anti-drug-antibodies
WO2017202643A1 (en) * 2016-05-23 2017-11-30 Koninklijke Philips N.V. Device for use in fluid sample analysis
EP3482199A1 (en) 2016-07-08 2019-05-15 Atonomics A/S A universal assay for determining the quantity of therapeutic monoclonal antibodies and their corresponding anti-drug-antibodies in samples

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US5053197A (en) * 1989-07-19 1991-10-01 Pb Diagnostic Systems, Inc. Diagnostic assay module
US5147606A (en) * 1990-08-06 1992-09-15 Miles Inc. Self-metering fluid analysis device
US5916522A (en) * 1997-08-07 1999-06-29 Careside, Inc. Electrochemical analytical cartridge
EP1882950A4 (en) * 2005-05-19 2012-08-15 Konica Minolta Med & Graphic TEST CHIP FOR ANALYZING TARGET SUBSTANCE IN SUBSTANCE TO BE ANALYZED AND ANALYTICAL SYSTEM AT MICROSCOPIC SCALE
WO2007004103A1 (en) * 2005-06-30 2007-01-11 Koninklijke Philips Electronics N.V. Cartridge for automated medical diagnostics

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EP2281631B1 (en) 2016-12-14

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