DK2170035T3 - Ændring af planters flavonoidmetabolisme - Google Patents
Ændring af planters flavonoidmetabolisme Download PDFInfo
- Publication number
- DK2170035T3 DK2170035T3 DK08757005.7T DK08757005T DK2170035T3 DK 2170035 T3 DK2170035 T3 DK 2170035T3 DK 08757005 T DK08757005 T DK 08757005T DK 2170035 T3 DK2170035 T3 DK 2170035T3
- Authority
- DK
- Denmark
- Prior art keywords
- leu
- lys
- gly
- ser
- arg
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Compounds Of Unknown Constitution (AREA)
- Peptides Or Proteins (AREA)
Claims (15)
- ÆNDRING AF PLANTERS FLAVONOIDMETABOLISME PATENTKRAV1. I alt væsentligt oprenset eller isoleret nukleinsyre eller nukleinsyrefragment, hvilken nukleinsyre eller hvilket nukleinsyrefragment koder for et MYB-protein, eller er komplementære eller antisense til en sekvens, der koder for et MYB-protein, og indbefatter en nukleotidsekvens udvalgt fra gruppen bestående af: (a) sekvenser vist i Figur 41,43 og 45 hertil (SEQ ID NO: 60, 62, 63 og 64); (b) komplementer af sekvenserne nævnt i (a); (c) funktionelt aktive fragmenter af sekvenserne nævnt i (a) og (b) med en størrelse på mindst 60 nukleotider; og (d) funktionelt aktive varianter med mindst ca. 95 % identitet med sekvenserne nævnt i (a), (b) og (c).
- 2. Nukleinsyre eller nukleinsyrefragment ifølge krav 1, hvor nukleinsyren eller nukleinsyrefragmentet er udvalgt fra gruppen bestående af: (a) sekvenser vist i Figur 41,43 og 45 hertil (SEQ ID NO: 60, 62, 63 og 64); og (b) funktionelt aktive varianter med mindst ca. 95 % identitet med sekvenserne nævnt i (a).
- 3. Nukleinsyre eller nukleinsyrefragment ifølge krav 1, hvor nukleinsyren eller nukleinsyrefragmentet indbefatter en nukleotidsekvens udvalgt fra gruppen bestående af sekvenser vist i Figur 41,43 og 45 hertil (SEQ ID NO: 60, 62, 63 og 64).
- 4. Konstrukt indbefattende en nukleinsyre eller et nukleinsyrefragment ifølge et hvilket som helst af kravene 1-3.
- 5. Vektor indbefattende en nukleinsyre eller et nukleinsyrefragment ifølge et hvilket som helst af kravene 1-3.
- 6. Vektor ifølge krav 5, hvilken vektor endvidere indbefatter en promoter og en terminator, hvilken promoter, nukleinsyre eller hvilket nukleinsyrefragment og hvilken terminator er operativt forbundet.
- 7. Plantecelle, plante, plantefrø eller anden plantedel indbefattende et konstrukt ifølge krav 4 eller en vektor ifølge krav 5 eller 6.
- 8. Plante, plantefrø eller anden plantedel, der er afledt af en plantecelle eller en plante ifølge krav 7 og indbefatter et konstrukt ifølge krav 4 eller en vektor ifølge krav 5 eller 6.
- 9. Fremgangsmåde til ændring af (a) flavonoiders biosyntese i en plante; (b) proteinbinding, metalchelatering, anti-oxidation og/eller UV-lysabsorption i en plante; (c) pigmentproduktion i en plante; (d) en plantes forsvar mod en biotisk stress; eller (e) en plantes foderkvalitet ved at bryde proteinskum og/eller bibringe beskyttelse mod græsningsbetinget trommesyge; hvilken fremgangsmåde indbefatter introduktion i planten af en effektiv mængde nukleinsyre eller nukleinsyrefragment ifølge et hvilket som helst af kravene 1-3, et konstrukt ifølge krav 4 og/eller en vektor ifølge krav 5 eller 6.
- 10. Fremgangsmåde ifølge krav 9, hvor fremgangsmåden ændrer en plantes forsvar mod en biotisk stress, og den biotiske stress er udvalgt fra gruppen bestående af vira, mikroorganismer, insekter og svampepatogener.
- 11. Anvendelse af en nukleinsyre eller et nukleinsyrefragment ifølge et hvilket som helst af kravene 1-3, og/eller nukleotidsekvensinformationer deraf, og/eller enkeltstående nukleotidpolymorfismer deraf som en molekylær genetisk markør.
- 12. I alt væsentligt oprenset eller isoleret MYB-polypeptid, hvor polypeptidet indbefatter en aminosyresekvens udvalgt fra gruppen bestående af: (a) sekvenser vist i Figur 42 og 46 hertil (henholdsvis SEQ ID NO: 61 og 65); (b) funktionelt aktive fragmenter af sekvenserne nævnt i (a) med en størrelse på mindst 50 aminosyrer; og (c) funktionelt aktive varianter med mindst ca. 98 % identitet med sekvenserne nævnt i (a) og (b).
- 13. Polypeptid ifølge krav 12, hvor polypeptidet indbefatter en aminosyresekvens udvalgt fra gruppen bestående af: (a) sekvenser vist i Figur 42 og 46 hertil (henholdsvis SEQ ID NO: 61 og 65); og (b) funktionelt aktive varianter med mindst ca. 98 % identitet med sekvenserne nævnt i (a).
- 14. Polypeptid ifølge krav 12, hvor polypeptidet indbefatter en aminosyresekvens udvalgt fra gruppen bestående af sekvenser vist i Figur 42 og 46 hertil (henholdsvis SEC ID NO: 61 og 65).
- 15. I alt væsentligt oprenset eller isoleret polypeptid kodet af en nukleinsyre eller et nukleinsyrefragment ifølge et hvilket som helst af kravene 1-3.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2007903525A AU2007903525A0 (en) | 2007-06-29 | Modification of plant flavonoid metabolism | |
| PCT/AU2008/000926 WO2009003216A1 (en) | 2007-06-29 | 2008-06-25 | Modification of plant flavonoid metabolism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2170035T3 true DK2170035T3 (da) | 2015-07-06 |
Family
ID=40225630
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15159902.4T DK2923562T3 (da) | 2007-06-29 | 2008-06-25 | Modifikation af planteflavonoidmetabolisme |
| DK08757005.7T DK2170035T3 (da) | 2007-06-29 | 2008-06-25 | Ændring af planters flavonoidmetabolisme |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15159902.4T DK2923562T3 (da) | 2007-06-29 | 2008-06-25 | Modifikation af planteflavonoidmetabolisme |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8633355B2 (da) |
| EP (2) | EP2170035B1 (da) |
| AU (1) | AU2008271901B2 (da) |
| CA (2) | CA2692257C (da) |
| DK (2) | DK2923562T3 (da) |
| NZ (3) | NZ591412A (da) |
| WO (1) | WO2009003216A1 (da) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2777249C (en) | 2009-10-16 | 2021-03-09 | Agriculture Victoria Services Pty Ltd | Manipulation of flavonoid biosynthetic pathway |
| US11421242B2 (en) | 2018-04-18 | 2022-08-23 | Pioneer Hi-Bred International, Inc. | Genes, constructs and maize event DP-202216-6 |
| CN109576284B (zh) * | 2018-12-21 | 2021-09-17 | 中国农业科学院北京畜牧兽医研究所 | 一个多功能的myb转录因子基因及其用途 |
| CN114621975B (zh) * | 2020-12-11 | 2023-05-02 | 华南农业大学 | 稻瘟病抗性相关基因OsWRKY5的应用 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5990386A (en) * | 1994-10-14 | 1999-11-23 | Washington State University Research Foundation | Genes controlling floral development and apical dominance in plants |
| US7345217B2 (en) * | 1998-09-22 | 2008-03-18 | Mendel Biotechnology, Inc. | Polynucleotides and polypeptides in plants |
| US20040031072A1 (en) * | 1999-05-06 | 2004-02-12 | La Rosa Thomas J. | Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement |
| US20110093981A9 (en) * | 1999-05-06 | 2011-04-21 | La Rosa Thomas J | Nucleic acid molecules and other molecules associated with transcription in plants and uses thereof for plant improvement |
| US20100293669A2 (en) * | 1999-05-06 | 2010-11-18 | Jingdong Liu | Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement |
| US20040181830A1 (en) * | 2001-05-07 | 2004-09-16 | Kovalic David K. | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
| AUPR811301A0 (en) | 2001-10-05 | 2001-10-25 | Agresearch Limited | Manipulation of flavonoid biosynthesis |
| US20050108791A1 (en) * | 2001-12-04 | 2005-05-19 | Edgerton Michael D. | Transgenic plants with improved phenotypes |
| US7396979B2 (en) * | 2004-06-30 | 2008-07-08 | Ceres, Inc. | Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics and phenotypes |
| US20060107345A1 (en) * | 2003-09-30 | 2006-05-18 | Nickolai Alexandrov | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
| US20060150283A1 (en) * | 2004-02-13 | 2006-07-06 | Nickolai Alexandrov | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
| US7569389B2 (en) * | 2004-09-30 | 2009-08-04 | Ceres, Inc. | Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics |
| US20060075522A1 (en) * | 2004-07-31 | 2006-04-06 | Jaclyn Cleveland | Genes and uses for plant improvement |
| US20080148432A1 (en) * | 2005-12-21 | 2008-06-19 | Mark Scott Abad | Transgenic plants with enhanced agronomic traits |
| NZ591569A (en) | 2005-07-20 | 2012-10-26 | Agriculture Victoria Serv Pty | Modification of flavonoid biosynthesis in plants with transparent testa 2 |
-
2008
- 2008-06-25 AU AU2008271901A patent/AU2008271901B2/en not_active Ceased
- 2008-06-25 EP EP20080757005 patent/EP2170035B1/en not_active Not-in-force
- 2008-06-25 CA CA2692257A patent/CA2692257C/en not_active Expired - Fee Related
- 2008-06-25 NZ NZ591412A patent/NZ591412A/xx not_active IP Right Cessation
- 2008-06-25 US US12/666,859 patent/US8633355B2/en not_active Expired - Fee Related
- 2008-06-25 CA CA2884680A patent/CA2884680C/en not_active Expired - Fee Related
- 2008-06-25 DK DK15159902.4T patent/DK2923562T3/da active
- 2008-06-25 NZ NZ602046A patent/NZ602046A/en not_active IP Right Cessation
- 2008-06-25 WO PCT/AU2008/000926 patent/WO2009003216A1/en active Application Filing
- 2008-06-25 EP EP15159902.4A patent/EP2923562B1/en not_active Not-in-force
- 2008-06-25 DK DK08757005.7T patent/DK2170035T3/da active
- 2008-06-25 NZ NZ581820A patent/NZ581820A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| US8633355B2 (en) | 2014-01-21 |
| AU2008271901A1 (en) | 2009-01-08 |
| EP2170035B1 (en) | 2015-04-22 |
| NZ591412A (en) | 2013-08-30 |
| NZ602046A (en) | 2014-07-25 |
| NZ581820A (en) | 2013-01-25 |
| EP2170035A4 (en) | 2011-01-05 |
| AU2008271901B2 (en) | 2015-01-22 |
| EP2923562A1 (en) | 2015-09-30 |
| DK2923562T3 (da) | 2019-06-11 |
| CA2692257C (en) | 2018-10-16 |
| CA2884680A1 (en) | 2009-01-08 |
| CA2692257A1 (en) | 2009-01-08 |
| EP2923562B1 (en) | 2019-03-13 |
| EP2170035A1 (en) | 2010-04-07 |
| WO2009003216A1 (en) | 2009-01-08 |
| CA2884680C (en) | 2019-09-03 |
| US20100186114A1 (en) | 2010-07-22 |
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