DK201400063U3 - Feed additive based on sodium gluconate or calcium gluconate in combination with one or more botanicals containing saponins and / or polyphenols - Google Patents

Abstract

Feed additives for use as feed additives to animals, comprising the mineral substance sodium gluconate or calcium gluconate, as well as saponin and / or polyphenol-containing elements from one or more plant materials, as well as any conventional adjuvants or additives. The additive is characterized in that it relates to the combination of sodium gluconate or calcium gluconate and by-products of industrial processing of one or more saponin- and polyphenol-containing botanical elements, and that it is used in small doses of 500-5000 ppm when admixed in a conventional finished feed, or as feed mixture. The additive can advantageously be used as an additive in feed for production animals, pet animals, mink, fish, crustaceans and seafood, as it improves production parameters and the overall health of the animals.

Description

Technical area

The present invention comprises an additive consisting of a mineral substance in combination with natural or natural identical active substances, as well as compound feed containing the additive.

Technical background

Antibiotic or chemotherapeutic agents are still used as so-called "growth promoters" in livestock production in large parts of the world. In recent years, however, attention has been increasingly directed at the fact that widespread use of antibiotics or chemotherapeutics causes problems. More and more pathogenic microorganisms are developing resistance to an increasing number of types of antibiotics, and there is also a risk that there are residues of antibiotics in food, so that humans also ingest these residues. In this way, pathogenic microorganisms can become resistant - even in humans.

Accordingly, it is now estimated that continued use of various antibiotics will increase the risk of epidemics that cannot be combated with antibiotics in humans and animals. Some people have already developed resistance to specific antibiotics, and one of the chemotherapeutic growth promoters used so far - Carbadox - has been shown to be carcinogenic.

This has meant that the use of the classic growth promoters in the EU is banned, and the use is now also widely debated in, among other things. The United States and several Asian countries.

Due to the aforementioned conditions, as well as to ensure the economy and sustainability of the livestock, alternative methods and means are still being sought to ensure the livestock a disease-free and effective outgrowth without the use of antibiotics.

Such agents may advantageously be based on natural or natural identical active substances as well as sodium gluconate or calcium gluconate. However, in order to ensure acceptance by both the farmer and the feed industry, such means should have a similar, or preferably better, effect on animal growth and feed utilization - and hence the emission of nitrogen - than the classical growth promoters. The requirements to minimize nitrogen emissions in agriculture are increasing these years - not least in Europe - and a very important factor in reducing it is improved feed utilization and, in particular, improved utilization of feed protein.

Not only is it a public requirement to reduce nitrogen emissions, but because of rising feed prices, it is also increasingly necessary for the farmer to improve the efficiency of animal feed utilization if livestock production is to be profitable.

1 Sodium gluconate or calcium gluconate in combination with one or more saponin- and polyphenol-containing plant parts is defined in this text as an additive in the broadest possible sense. However, it should be noted that several of the elements mentioned above are defined as feed materials in the EU, cf. COMMISSION REGULATION (EU) No 68/2013.

It is also known that improved efficiency of animal feed utilization in itself reduces the need for animal medication, including antibiotic medication.

In this regard, it is important that sodium gluconate2 can influence production parameters in livestock production by, inter alia, improving the prebiotic activity in the small intestine and thereby improving the overall health of the animals. Sodium gluconate has also shown an ability to promote the production of short-chain fatty acids in the intestinal tract, which ensures an increased energy supply to the animals. In addition, the height and depth of the gut are increased. (Poeikhampha & Bunchasak, 2011A + B.), (Biagi et al., 2006)

Calcium gluconate also has effective properties in livestock production. Thus, it has long been a well-known means of controlling milk fever in dairy cattle, which occurs at low concentrations of calcium in the blood (Hibbs, 1950).

The botanicals contain various functional and / or antibacterial agents, including saponins and polyphenols, including flavonoids. It is also known that such active substances from plant materials can be used in and as pharmaceuticals. The positive effects of some of these active substances are due, in whole or in part, to their antioxidant properties, which protect cells from degradation and harmful substances, reduce infectious risks, and have special effects at high production levels and consequently high stress levels in animals.

Flavonoids are a group of secondary plant metabolites, many of which act as antioxidants. Among other things. flavonoids have the ability to inhibit the oxidation of LDL (Low-Density Lipoproteins) and thus protect against cardiovascular disease (Heim et al., 2002). In addition, studies also show that flavonoids have anti-inflammatory properties as they have been shown to reduce the production of two classes of pro-inflammatory factors - prostaglandin and leukotriene - believed to be contributing to the development of COPD (Tobacco Chronic Disease) (Tobacco et al., 2001). In studies of people consuming flavanol-containing foods, individuals have been shown to have an improved immune system and protection against free radicals (Schroeter et al., 2005). In foods, the highest concentrations of flavonoids are found in fruits and berries, vegetables, grapes and tea (Heim et al., 2002).

There are various patents and patent applications which include, inter alia, the inclusion of sodium gluconate (glutamic acid) and calcium gluconate; for example, as a feed supplement for production animals, for the purpose of improving selected production parameters (US6718910); glutamic acid to improve creatine storage in animals and humans (US8506989B2); glutamic acid for the treatment of skin keratosis (US4234599); glutamic acid in feed for piglets (US4362710); and sodium gluconate for the production of zerofiber feed (US4954355A); methods of preparing calcium gluconate (US 7618664 B2); methods for preparing easily soluble calcium gluconate (US 1900517 A).

In general, there are many patents and patent applications covering the use of sodium gluconate in the form of glutamic acid, as well as calcium gluconate - also in relation to production animals. Although the beneficial effects of 2 Sodium gluconate = sodium salt of the d-glutamic acid mineral substance are well documented and already used in livestock production, however, no patents or patent applications include the use of sodium gluconate or calcium gluconate in combination with one or more botanical elements as an additive in feed.

In v / Vo experiments have now shown that the combination of sodium gluconate and very small amounts of selected by-products from the industrial utilization of various botanical elements has a production-enhancing effect when added to the feed for production animals. This reduces the animals' need for antibiotic medication and improves the efficiency of their feed utilization. In addition, the production-enhancing effects of the combination product appear to outweigh the production-promoting effects by including either sodium gluconate or the botanical elements separately.

Brief Description of the Invention

The present invention relates to an additive for use as feed in feed for pets and mink, fish, seafood and crustaceans and containing the following elements: 1) Sodium gluconate or calcium gluconate 2) Parts of botanical elements having antioxidant properties which can be obtained from a plant material preferably derived from Olives (Olea europaea), Vin (vitis vinifera), Tea (Camellia spp., preferably C. sinensis), Citrus (citrus spp.) and / or parts of various berries, preferably Blackcurrants (Ribes nigrum) , Blackberry (Sambucus spp.), Blueberry (Vaccinium myrtillus), Cranberry (Vaccinium oxycoccus; V.microcaroum; V.macrocarpum), Aronia berry (aronia spp., Special aronia arbutifolia; aronia melanocarpa), cranberry (Vaccinium vitis idaea) , and / or parts of the Lip Flower family (Lamiaceae), preferably Rosemary (Rosmarinus officinalis), Thyme (Thymus vulgaris L), Oregano (origanum vulgare), Sage (Salvia) and Melisse (Melissa officinalis), and / or parts of botanis ke elements with surfactants from Yucca (mainly schidigera), Kvillaja (Quillaja saponaria), Quinoa (Chenopodium guinoa), Soap (Saponaria officinalis), Tea (Camelia oleifera), Buckhorn ((Trigonella foenum), Grape) Vitellaria paradoxa) 3) Any conventional additives or additives.

The invention further includes livestock feed mixtures comprising the additive together with a conventional feed.

The scope of the applicability of the invention will become apparent from the following detailed description. It should be noted, however, that the detailed description and specific examples are provided by way of illustration only, indicating preferred embodiments of the invention. Various changes and modifications within the scope of the invention will become apparent to those skilled in the art based on the detailed description.

Detailed description of the invention

Sodium gluconate is an animal feed based on a mineral salt consisting of sodium salt of d-glutamic acid. The glutamic acid is derived from glucose by a fermentation process. The analytical constituents are sodium and gluconic acid. The feed has so far been used for admixture in animal feed, most often at an inclusion rate of 2000-5000 ppm.

Several in vivo experiments with sodium gluconate (glutamic acid) have shown a very effective influence on the production parameters in livestock production. Among other things. sodium gluconate has improved the overall health of the animals by improving the prebiotic activity of the small and large intestine, and the substance has also shown an ability to promote the production of short-chain fatty acids in the intestinal tract, which ensures an increased energy supply to the animals. In pigs trials, the product has also shown an ability to increase the height and depth of the gut villi (Poeikhampha & Bunchasak, 2011A + B.) (Biagi et al.).

Calcium gluconate has long been a well-known means of controlling milk fever in dairy cattle. As early as 1930, a successful trial was reported in which calcium gluconate was given intravenously in the control of milk fever. This revolutionized the treatment of the problem as it replaced the treatment methods of the past and the problems associated with it (Hibbs, 1950).

The botanical elements according to the invention are selected from various by-products from, for example, olive oil, tea seed oil, shea oil or juice production or on the basis of leaves from, for example, olives, rosemary etc. These products have a high content of antioxidants and / or saponins which are considered as essential in the development of animal feed additives. The following will cover a detailed description of selected botanical elements so that the four groups above; plant materials, berries, the lip flower family and saponin-containing elements are all being reviewed.

The olive is the fruit of the olive tree (Olea europaea L). The production of olive oil is carried out by washing olive fruit, after which they are ground in a hammer mill. The resulting "pomace", which is a mixture of olive stones and olive pericarp (the flesh itself), is homogenized and transferred to press plates where the oil is pressed out. The remaining olive press residue has no other industrial use, but still contains a large amount of antioxidants. The same is true for the leaves of the olive tree which have a high content of antioxidants. These are not utilized in a larger scale industrially, but extracted from them are extracts useful in this invention.

Another possible component of the additive is obtainable on the basis of a by-product of wine production, which may consist of the total press residue - ie. of anything but the grape juice. This press residue (also called grape pomace or grape marc) is traditionally utilized as a raw material for the production of a variety of products. The remainder of the press can subsequently be further processed. Thus, it is technically possible to separate the cores from the skin for oil recovery from the cores. Both the residues from the oil extraction and the separated skin are suitable press residue products which can be included in the additive according to the invention.

In addition, additive components may be obtainable on the basis of dried milled portions of olive leaves, citrus peel or similar elements of the said plants and fruits.

Other possible components of the additive according to the invention are obtainable on the basis of a by-product of berries or citrus from the production of foods, especially jelly and juice, based on the aforementioned berries or on the basis of the leaves.

In the production of jelly and juice, a by-product, called press residue, arises. This press residue consists of shells of berries, kernels and possibly. flesh. The residue of the press has a high content of antioxidants, as is the case for leaves from shrubs and trees of blueberries and blackberries as well as elderberries and cranberries. Press residues can either be used directly or extracts can be extracted from them.

Components for the additive can also be extracted from the lip flower family (Lamiaceae), preferably leaves and stems from rosemary (rosmarinus officinalis), thyme (thymus vulgaris L), oregano (Origanum valgare), sage (salvia) and melissa (Me // ssa officinalis).

Active elements of the invention are also obtainable on the basis of saponin-containing elements - i. from tea. Tea belongs to the genus Camellia, belonging to the Theaceae (Camellia family), which is divided into 2 subfamilies, Theoi-deae and Ternstroemioideae, where Camellia belongs to the first subfamily. The family contains approx. 40 genera and 600 species. The Camellia genus includes 100-250 species. The commonly known tea that is consumed by humans is made by C. sinensis. The species C. oleifera, on the other hand, is grown for the sake of seeds because of their high content of oil, which can be extracted by pressing. This oil is used in much of Asia as a very healthy cooking oil. Other large amounts of the oil are used in the pharmaceutical industry as well as in cosmetics.

The residual presses after the oil extraction are dried and called teacake. Typically, C. oleifera is used in oil production, but also C. sinensis, C. chekiangoleosa, C. drupifera, C. reticulata, C. crapnelliana, C. sasangua and C. japonica can be used for this purpose and are covered by this utility model. The invention includes both tea seed cake (Camelia oil fera), obtained after the oil extraction or a water extract thereof, as well as other saponin-containing by-products based on tea elements.

A possible active component may also be derived from the quillaja obtained from the soap bark tree (Quillaja saponaria), from the branches and bark of the tree which is ground and dried or used as an extract. The extract may be based on pure water extraction or on a mixture of water and alcohol as extractants. The extract can be used as a liquid product or it can be dried, e.g. spray dried.

In addition, one component may be Quinoa (Chenopodium guinoa). Quinoa has been cultivated since 5000 BC. and is thus one of the oldest crops in the world. It is one of the most important crops in the Andean region, where it is used as a food of high nutritional value. The quinoa seed contains a core covered in a bitter shell with a high saponin content which is removed before the quinoa can be used in cooking and for feeding. It is estimated that 10% of the harvested yield is made of shell flour. The shell is removed by grinding by mechanical action. The saponins can also be washed out of the untreated quinoa (including shell), using water. The saponin content is estimated to be 10-15% in the oatmeal.

Also saponin-containing components derived from e.g. fenugreek (Trigonella foecum graecum), licorice (Glycyrrhiza glabra), yucca (Yucca spp.) shea (Vitellaria paradoxa) or soap herb (Saponaria officinalis) may be used.

The components can be used either as whole elements or as extracts of the whole elements or of the by-products. The extracts may be extracted by water, alcohol or a combination thereof.

The invention contains a number of interesting active substances. Among the most important are saponins, which provide a surface-relaxing effect, as well as polyphenols with antioxidant properties as well as the anthocyanins and oligomeric procyanidins subgroups.

It is well known that, for example, olives have a number of health-promoting effects, such as lower risk of cardiovascular disease and cancer, which has been partly explained by the high content of natural antioxidants. These active substances have shown biological activity, including cell growth inhibition of cancer cells, as well as antiviral and antioxidant activity (e.g., inhibition of oxidation of low-density lipoproteins (LDL) and inhibition of oxidative stress). The antioxidant activity of active substances found in olives is far more effective than a synthetic form of vitamin E (Owen et al, 2000). Antifungal activity has also been found (Del Rio et al, 2003).

It is also well known that both wine and by-products from wine production have a number of health-promoting effects. Wine includes phenols such as pigments and tannins, as well as organic acids and vitamins. Known health-promoting effects of wine include: antiviral, antiallergic, antioxidant, and cancer-inhibiting (Bombardelli & Morazzoni, 1995).

Different berries also have a high content of polyphenols with antioxidant capacity (Kahkonen et al., 2001). The carriers include anthocyanins, flavonols, routine and catechins. Studies have shown the potential of berries in the fight against eg. cancer and arteriosclerosis, and the mechanisms are believed to be based on the ability of polyphenols to fight oxidative stress and inflammatory conditions (Neto, 2007). Cranberries also contain procyanidins, which are thought to reduce the attachment of pathogenic bacteria and toxins to the epithelium and therefore have a beneficial effect against, for example, urinary tract infections (Duthie, 2007). Blackberries have shown antiviral effects and ability to stimulate the immune system. They are thought to be effective against influenza (Wu et al., 2005).

The Lip Flower family (Lamiaceae) of rosemary (Rosmarinus officinalis), which was one of the first natural antioxidants to be used commercially. The antioxidant effect of rosemary is derived from 16 active substances, including diterpenes and phenolic acids, which help to prolong the shelf life and stability of food by preventing the oxidation of molecules (Crowley, L. 2008). Rosemary also has a strong antibacterial effect, which makes it effective against both Gram and Gram + bacteria, including E. coli, salmonella and streptococci (Oluwatuyi et al, 1994). Rosemary's strong aroma also enhances the appetite of animals. are particularly important for newborns, as well as for sick, or otherwise weak, animals.

A wide variety of botanicals contain saponins that have a proven antibacterial and antifungal effect on i. E.coli, Salmonella typhimurium and Candida albicans (Sen et al. 1998). In addition, saponins are known to have an immune-stimulating effect in the gastrointestinal system, resulting in improved resistance to intestinal infections (Turner et al. 2000). Saponins consist of both steroidal saponins (for example, from yucca and buckshorn) as well as triterpene saponins (for example, from quillaja, quinoa and tea), the latter having the strongest surface relaxant effect.

In addition to the above elements, additional carriers may be used. In this connection, suitable carriers are primarily mineral carriers in the form of clay minerals and zeolites - especially sepiolite and clinoptilolite - as well as diatomaceous earth. Secondary vegetable carriers such as wheat flour, wheat bran, soybean meal, etc. Calcium carbonate or calcium magnesium carbonate (dolomite lime) may also be useful carriers. The same goes for grapefruit flour, which is a known carrier, frequently used in animal feed.

In contrast to various other additives, the additive according to the invention is extremely heat stable, which is why it is also suitable for admixture in the pelleted and extruded feed. It can thus be used with great yield in feed for both domestic animals and ruminants, fish, crustaceans and seafood as well as for pet animals incl. horses, dogs and cats.

The additive works by improving the quality of animal production on a wide range of parameters. Among other things, the quality of the meat is improved, including the color, structure and stability, thereby extending the shelf life and reducing the drip loss. In poultry production, the quantity and quality of the eggs produced, including the color of the flower, the thickness of the egg shells, and the overall shelf life of the eggs are improved. In addition, mortality of young animals, incl. chickens, piglets, seafood.

The additive improves the overall health of the animals and thus reduces the need for medical treatment - also with types of medicine that are dangerous to humans and to humans and animals.

The production is characterized by having a very fast measurable effect when incorporated into animal feed. In newly hatched chicks, this surprising effect can be measured at a highly significant level even after just 10 days. In piglets, a statistically significant effect can also be measured already after just 10-14 days. In addition, after inclusion for 21 days, a significant effect is still observed.

This makes production extremely relevant as it is effective even if mixed only in pre-starting and starting feed for very young animals, and not just in feed for production animals of all ages - unlike many existing production-promoting additives and feed materials on the international market. .

The production is an inexpensive and extremely cost-effective product that, when mixed into animal feed, increases the efficiency and the animal productivity quite significantly.

The additive according to the invention has been found in practical experiments to have a surprisingly significant effect on the daily growth and feed utilization of the livestock. Experimental results will be presented on the following pages, which will refer to a Danish trial of newly weaned pigs, as well as an international trial of broilers.

Experiment with the additive Experiment 1

The example below is based on the use of the product "Product B" which consists of a variety of botanicals containing antioxidants and saponins with specific properties. To verify the additive, a trial of newly weaned pigs was conducted.

The trial consisted of 60 pigs and lasted for 21 days. The pigs were divided into two groups of 30 pigs - one control group and one experimental group. The latter was added to Product B at an inclusion rate of 15 ppm. The pigs were weighed at trial start, at 11 days and at trial end. In the first period (from 1-11 days) there were major problems with E.coli in the herd. These decreased in the second period (from 12-21 days).

The table below shows the results for each period as well as for the entire trial of the production parameters average daily growth (ADG), average daily feed intake (ADFI), and the efficiency of feed utilization (FCR).

To hol 1 - Roe 111 to tor fnr nrnrli ilrtinnenoromotrono ΔΠί'ί Δ ΠΕΙ eomtPPR Hao 1.11

Table 2 - Results for the production parameters ADG, ADFI, and FCR, days 12-21

Table 3 - Results for the production parameters ADG, ADFI, and FCR, days 0-21

The experiment showed that Product B had an efficient and measurable effect on all the production parameters. After 21 days, ADG improved by 35%, ADFI by 25% and FCR by 8%.

The product shows a very immediate effect as inclusion after 11 days improved the above parameters by 44%, 7% and 21% respectively. This also suggests that Product B is highly effective against E.coli, as it has managed to inhibit its adverse effects. The control group has thus not been able to stand against E. coli, especially the feed utilization of 2.81 kg feed per day. kg of growth is very poor.

In period 2, when the adverse effects of E.coli subsided, there was still a significant improvement in ADG (34%) and ADFI (30%), while FCR only improved by 3%. The large difference in feed intake, and hence the growth in growth, is believed to be justified by the poor start the control group got right after weaning. This shows the importance of adding Product B to the feed as early as possible.

Experiment 2

The example below is based on the use of the product "Product NB" which consists of a combination of sodium gluconate and a variety of botanicals containing antioxidants and saponins with specific properties. For the verification of the additive, broiler chickens were conducted during the period from hatching to slaughter.

The trial consisted of 240 Ross-308 broilers. They were divided into four groups; one experimental group and three control groups equally distributed by gender. Each group consisted of 5 repetitions of 12 chickens. The experimental groups were added sodium gluconate alone (Product N) at an inclusion rate of 2500 ppm, respectively, the botanical elements alone (Product B) at an inclusion rate of 15 ppm, and a combination of the two products "Product NB" at an inclusion rate of 2515 ppm. The chickens were weighed 3 times during the trial; at trial start, and after 10 and 21 days.

The table below shows the results after each weighing on the production parameters average daily growth (ADG), average daily feed intake (ADFI), and the efficiency of feed utilization (FCR).

Table 4 - Results for the production parameters ADG, ADFI, and FCR

II Significance level: Δ P <0.1, * P <0.05, ** P <0.01, *** P <0.001, NS - not significant

The experiment showed that sodium gluconate (Product N) has a positive, albeit not significant, effect on the production parameter FCR. For ADG, there was a less significant effect after 10 days and for the entire period, but not for the period from 11-21 days. The effect is also less significant than for both the botanical elements alone and for the combination product.

The botanicals (Product B) showed a significant effect on ADG and FCR after both 10 and 21 days, but not for the period from 11-21 days. Thus, there is a rapid measurable effect of inclusion of the botanical elements alone, as both FCR and ADG were significantly improved after just 10 days.

The combination product (Product NB) showed better results than the two individual items separately at both 10 and 21 days. The product thus produced an even better and more significant result than by the inclusion of the botanical elements alone, and the effect was both stronger but also proved faster than by the inclusion of sodium gluconate alone. This suggests that the botanical elements have a not only additive but also synergistic effect in combination with sodium gluconate.

references:

Biagi G., Piva, A., Moscini M., Vezzali, E., Roth, F. X., 2006, Effect on gluconic acid on piglet growth performance, intestinal microflora and intestinal wall morphology, Journal of Animal Science

Bombardelli & Morazzoni, 1995: Vitis Vinifera L. Fitoterapia LXVI (4)

Crowley Laura, 2008; Rosemary extracts to receive antioxidant status, Global Meat News

Del Rio et al., 2003: Enhancement of phenolic compounds in olive plants and their influence on resistance against Phytophthora sp. Food Chemistry 83 (1), pp. 75-78

Duthie, 2007: Berry fruits. Molecular Nutrition & Food Research 51, p 643

Heim et.al. (2002). Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. Journal of Nutritional Biochemistry 13, pp. 572-584.

Hibbs, 1950, Milk fever (parturient paresis) in dairy cows, Journal of Dairy Science, Vol 33, Issue 10, Pages 758-789

Kahkonen et al., 2001: Berry phenolics and their antioxidant activity. Journal of Agricultural and Food Chemistry 49, pp. 4076-4082)

Neto, 2007: Cranberry and blueberry: Evidence for protective effects against cancer and vascular diseases. Molecular Nutrition & Food Research 51, pp. 652-664)

Oluwatuyi, M., Kaatz, G. W., Gibbons S., 1994, Antibacterial and resistance modifying activity of rosmarinus officinalis, Elsevier

Owen et al., 2000: Olive-oil consumption and health: The possible role of antioxidants. The Lancet Oncology 1, pp. 107-112

Poeikhampha, T. & Bunchasak C. (2011A): A dietary sodium gluconate supplement improves growth performance and prebiotic activity in the small intestine of nursery pigs under tropical conditions, Animal Production Science

Poeikhampha, T. & Bunchasak C. (2011B): Comparative effects of Sodium Gluconate, Mannan Oligosaccharide and Potassium Diformate on growth performances and small intestinal morphology of nursery pigs, Asian-Aust. J Animal Science

Sen, S., Makkar, Η. P. S., Muetzel, S., and Becker, K., 1998, Effect of Quillaja saponaria saponins and Yucca schidigera plant extract on growth of Escherichia coli. Letters in Applied Microbiology 27 (1), 35-38.

Schroeter it. eel. (2005). Epicatechin mediates beneficial effects of flavanol-rich cocoa on vascular function in humans. PNAS103 (4), 1024-1029.

Tobacco et al. (2001). Chronic Obstructive Pulmonary Disease and Intake of Catechins, Flavonols and

Flavones. AmericcmJournal of Respiratory and Critical Care Medicine 164, 61-64.

Wu et al., 2005: Aglycones and sugar moieties alter anthocyanin absorption and metabolism after berry consumption in weanling pigs. The Journal of Nutrition 135, pp. 2417-2424).

Turner et al. (2000): Effects of a Quillaja saponaria extract on weanling pig growth performance and immune function during an acute enteric disease challenge, Swine day

Patent references: • US6718910 »US8506989B2» US4234599 »US4362710

• US4954355A

• US 7618664 B2

• US 1900517 A

Claims (3)

1. Natural additive3 for use as a supplement or additive in animal feed and containing natural and / or natural identical active substances, including sodium gluconate or calcium gluconate and one or more botanical elements, and any conventional additives or additives. The additive of claim 1, which is new in that it contains a combination of sodium gluconate or calcium gluconate and one or more botanicals containing saponins and / or polyphenols, preferably from quillaja (Quillaja saponaria), quinoa (Chenopodium quinoa), tea (Camellia spp.), buckthorn (Trigonella foecum graecum), licorice (Glycyrrhiza glabra), yucca (Yucca spp.), shea (Vitella-ria paradoxa), from Olives (Olea europaea), Vin (vitis vinifera), Tea ( Camellia spp., Preferably C.sinensis), Citrus (citrus spp.), Blackcurrant (Ribes nigrum), Blackberry (Sambucus spp.), Rosemary (rosma-rinus officinalis), Timian (thymus vulgaris L), Oregano (origanum vulgare), Sage (salvia) and Melisse (Me // ssa officinalis), or extracts of one or more of these. An additive according to any one of the preceding claims, new in that it comprises a feed acceptable for feed. The additive of claim 1, which is new in that it is added to the feed by inclusion of 500-5000 grams per day. tonnes of feed (500-5000 ppm), measured on the dry matter content of the feed and on the dry matter content of the element. Animal feed compositions novel by including the ingredients of the additive according to any one of the preceding claims together with a conventional feed. An additive according to any one of the preceding claims, new in that it is offered in both solid and liquid form. Additive according to any one of the preceding claims, new in that it is used as a direct supplement to the animal via bolus, capsule, tablet, drinking water, ointment, spray, paste or gel. Additive according to any one of the preceding claims, which is new in that it is used for all kinds of monogastric animals, production and pets, fish, seafood, crustaceans, shrimp and mink. Additive according to any one of the preceding claims, which is new in that, when included in animal feed, it has a rapidly measurable effect on a wide range of production parameters. 3 Sodium gluconate or calcium gluconate in combination with one or more saponin- and polyphenol-containing plant parts is defined in this text as an additive in the broadest possible sense. However, it should be noted that several of the elements mentioned above are defined as feed materials in the EU, cf. COMMISSION REGULATION (EU) No 68/2013.