DK200101777A - Protease for oral care is substantially inactive in natural mouth environment when oral care composition is absent - Google Patents

Protease for oral care is substantially inactive in natural mouth environment when oral care composition is absent Download PDF

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DK200101777A
DK200101777A DK200101777A DKPA200101777A DK200101777A DK 200101777 A DK200101777 A DK 200101777A DK 200101777 A DK200101777 A DK 200101777A DK PA200101777 A DKPA200101777 A DK PA200101777A DK 200101777 A DK200101777 A DK 200101777A
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strain
derived
oral care
protease
bacillus
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DK200101777A
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Novozymes As
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Abstract

The oral care composition comprises a protease which is substantially inactive in the natural mouth environment when the oral care composition is absent.

Description

CLAIMS 1. An oral care composition comprising a protease, characterized in that the protease is substantially inactive in the natural environment prevailing in the mouth when the oral care composition is absent. 2. A composition according to claim 1, wherein the composition is a composition according to p. 18-19,or the tables on p. 25-29, or a composition according to p. 40-50 (examples 1-17 and tables), or a composition according to p. 57-62 (examples 1-9 and tables). 3. A composition according to any of the preceding claims, wherein the protease is substantially inactive in the natural pH- environment prevailing in the mouth when the oral care composition is absent. 4. A composition according to any of the preceding claims, wherein the protease is substantially inactive in the close range of about 7, preferably with the range of about pH 6.4 to 7.0, even more preferred between about pH 6 to 8. 5. A composition according to any of the preceding claims, wherein the protease is derived from a strain of Bacillus sp., in particular a strain of Bacillus subtilis, or fungal strains of a Rhizomucor sp., in particular a strain of a Rhizomucor miehei, or a strain of Rhizopus sp., in particular a strain of R. nlveus, or a strain of a Scytalldlum sp., or a strain of a Sulpholobus sp., or a strain of a Tharmoplasma sp., or a strain of a Aspergillus sp., in particular a strain of A. aculeatus, or A. rtlger, or A. awamori, or A. oryzae, or a strain of a Trichoderma sp., in particular a strain of T. harzlanum, or T. nees/e, or a strain of a Fusaiium sp., in particular a strain of F. oxyspomm, or a strain of a Humicola sp., such as subtilisin BPN\ subtilisin amylosaccheriticus, subtilisin 168, subtiiisln mesentericopeptidase, subtilisln Carlsberg, subtilisin DY, subtilisin 309, subtilisin 147, thermitase, aqualysin, Bacillus ΡΒΘ2 protease, proteinase K, Protease TW7, Protease TW3, such as Esperase®, Alcalase®, Neutrase®, Dyrazym®, Savinase®, Pyrase®, Pancreatic Trypsin NOVO (PTN), Bio-Feed® Pro, Clear-Lens Pro, Maxtase®, Maxacal®, Maxapem® marketed by DSM, Opticlean® or Purafect®. 6. A composition according to any of the preceding claims, wherein the protease is SEQ ID No. 1 or SEQ ID No.2 disclosed in WO 95/02044 also called protease I and li, respectively. 7. A composition according to any of the preceding claims wherein the protease is a mucor pepsin derived from a strain of Rhizomucor, such as R. mlehel. 8, A composition according to any of the preceding claims wherein the composition comprises further enzymes such as a lipase, In particular a lipase derived from Humicola, e.g. H. brevispora, H. lanuginosa, H. brevis var. thermoidea or H, Insolens or Pseudomonas, e.g. Ps. fragi, Ps. stutzerl, Ps. cepacia or Ps. fluorescens, or Ps. plantaril or Ps. gladioli or Ps. alcaligenes or Ps. pseudoalcaligenes or Ps. mendoclna or Fusarium, e.g. F. oxysporum or F. solani pisi or Mucor (also called Rhizomucor), e.g. M. miehel or Chromobacterium, e.g. C. viscosum or Aspergillus, e.g. A. niger or Candida, e.g. C. cylindracea (also called C. rugosa) or C. antarctica or C. antarctica lipase A or B or Geotricum, e.g. G. candidum or Penlcilllum, e.g. P. camembertil or Rhizopus, e.g. R. delemar or R. niveus or R. oryzae or Bacillus, e.g. S. subtilis or B. staarothermophilus or B. pumilus other examples Include Lipolase®, Lipolase® Ultra, Lipozyme®, Palatase®, Novozym® 435, Lecitase®, Lumafast®, Ps. mendocian lipase from Genencor Int. Inc.; Llpomax®, Ps. pseudoalcaligenes lipase from DSM/Genencor Int. Inc.; Fusarium solani lipase (cutinase) from Unilever; Bacillus sp. lipase from Genencor Int. enzymes or a carbohydrase such as a alpha-1,3-glucanase derived from Trichoderma harzlanum\ alpha-1,6-glucanases derived from a strain of Paecilomyces·, beta-glucanases derived from Bacillus subtilis·, beta-glucanases derived from Humicola insolens-, beta-glucanases derived from Aspergillus niger, beta-glucanases derived from a strain of Trichoderma-, β-glucanases derived from a strain of Oerskovia xanthineolytica; exo-1,4-alpha-D-glucosidases (glucoamylases) derived from Aspergillus niger, alpha-amylases derived from Bacillus subtilis-, alpha-amylases derived from Bacillus amyioliquefaclens; alpha-amylases derived from Bacillus stearothermophilus-, alpha-amylases derived from Aspergillus oryzae- alpha-amylases derived from non-pathogenic microorganisms; alpha-galactosidases derived from Aspergillus niger, Pentosanases, xylanases, cellobiases, cellulases, hemi-cellulases derived from Humicola insolens] cellulases derived from Trichoderma reesei; cellulases derived from non-pathogenlc mold; pectinases, cellulases, arabinases, hemi-celluloses derived from Aspergillus niger, dextranases derived from Peniclliium lilacinum-, endo-glucanase derived from non-pathogenic mold; pullulanases derived from Bacillus acidopullyticus; beta-galactosidases derived from Kluyveromyces fragllis; xylanases derived from Trichoderma reesei other examples include BAN™, MaFtogenase™, an alpha-amylase derived from Bacillus subtilis·, an alpha-amylase derived from Bacillus amyioliquefaclens-, an alpha-amylase derived from Bacillus stearothermophilus; an α-amylase derived from Aspergillus oryzae] an alpha-amylase derived from a non-pathogenic microorganism, Fungamyl™ or a CGTase, such as a CGTase derived from a strain of Bacillus autolyticus, a strain of Bacillus cereus, a strain of Bacillus circulars, a strain ofCLAIMS 1. An oral care composition comprising a protease, characterized in that the protease is substantially inactive in the natural environment prevailing in the mouth when the oral care composition is absent. 2. A composition according to claim 1, wherein the composition is a composition according to pp. 18-19, or the tables on pp. 25-29, or a composition according to pp. 40-50 (examples 1-17 and tables ), or a composition according to pp. 57-62 (examples 1-9 and tables). A composition according to any of the preceding claims, wherein the protease is substantially inactive in the natural pH-environment prevailing in the mouth when the oral care composition is absent. A composition according to any of the preceding claims, wherein the protease is substantially inactive in the close range of about 7, preferably with the range of about pH 6.4 to 7.0, even more preferred between about pH 6 to 8. 5. A composition according to any of the preceding claims, wherein the protease is derived from a strain of Bacillus sp., in particular a strain of Bacillus subtilis, or fungal strains of a Rhizomucor sp., in particular a strain of a Rhizomucor miehei, or a strain of Rhizopus sp., in particular a strain of R. nlveus, or a strain of a Scytalldlum sp., or a strain of a Sulpholobus sp., or a strain of a Tharmoplasma sp., or a strain of a Aspergillus sp. , in particular a strain of A. aculeatus, or A. rtlger, or A. awamori, or A. oryzae, or a strain of a Trichoderma sp., in particular a strain of T. harzlanum, or T. nees / e, or a strain of a Fusaiium sp., in particular a strain of F. oxyspomm, or a strain of a Humicola sp., such as subtilisin BPN \ subtilisin amylo saccheriticus, subtilisin 168, subtiiisln mesentericopeptidase, subtilisln Carlsberg, subtilisin DY, subtilisin 309, subtilisin 147, thermitase, aqualysin, Bacillus ΡΒΘ2 protease, proteinase K, Protease TW7, Protease TW3, such as Dyperase®, Alcal® , Savinase®, Pyrase®, Pancreatic Trypsin NOVO (PTN), Bio-Feed® Pro, Clear-Lens Pro, Maxtase®, Maxacal®, Maxapem® marketed by DSM, Opticlean® or Purafect®. A composition according to any of the preceding claims, wherein the protease is SEQ ID No. 1 or SEQ ID No.2 disclosed in WO 95/02044 also called protease I and li, respectively. 7. A composition according to any of the preceding claims wherein the protease is a mucor pepsin derived from a strain of Rhizomucor, such as R. mlehel. 8, A composition according to any of the preceding claims wherein the composition comprises further enzymes such as a lipase, In particular a lipase derived from Humicola, e.g. H. brevispora, H. lanuginosa, H. brevis var. thermoidea or H, Insolens or Pseudomonas, e.g. Ps. fragi, Ps. stutzerl, Ps. cepacia or Ps. fluorescence, or Ps. plantaril or Ps. gladioli or Ps. alcaligenes or Ps. pseudoalcaligenes or Ps. mendoclna or Fusarium, e.g. F. oxysporum or F. solani pisi or Mucor (also called Rhizomucor), e.g. M. miehel or Chromobacterium, e.g. C. viscosum or Aspergillus, e.g. A. niger or Candida, e.g. C. cylindracea (also called C. rugosa) or C. antarctica or C. antarctica lipase A or B or Geotricum, e.g. G. candidum or Penlcilllum, e.g. P. camembertil or Rhizopus, e.g. R. delemar or R. niveus or R. oryzae or Bacillus, e.g. S. subtilis or B. staarothermophilus or B. pumilus other examples Include Lipolase®, Lipolase® Ultra, Lipozyme®, Palatase®, Novozym® 435, Lecitase®, Lumafast®, Ps. mendocian lipase from Genencor Int. Inc .; Llpomax®, Ps. pseudoalcaligenes lipase from DSM / Genencor Int. Inc .; Fusarium solani lipase (cutinase) from Unilever; Bacillus sp. lipase from Genencor Int. enzymes or a carbohydrase such as an alpha-1,3-glucanase derived from Trichoderma harzlanum \ alpha-1,6-glucanases derived from a strain of Paecilomyces ·, beta-glucanases derived from Bacillus subtilis ·, beta-glucanases derived from Humicola insolens , beta-glucanases derived from Aspergillus niger, beta-glucanases derived from a strain of Trichoderma-, β-glucanases derived from a strain of Oerskovia xanthineolytica; exo-1,4-alpha-D-glucosidases (glucoamylases) derived from Aspergillus niger, alpha-amylases derived from Bacillus subtilis-, alpha-amylases derived from Bacillus amyioliquefaclens; alpha-amylases derived from Bacillus stearothermophilus-, alpha-amylases derived from Aspergillus oryzae- alpha-amylases derived from non-pathogenic microorganisms; alpha-galactosidases derived from Aspergillus niger, Pentosanases, xylanases, cellobiases, cellulases, hemi-cellulases derived from Humicola insolens] cellulases derived from Trichoderma reesei; cellulases derived from non-pathogenlc mold; pectinases, cellulases, arabinases, hemi-celluloses derived from Aspergillus niger, dextranases derived from Peniclliium lilacinum-, endo-glucanase derived from non-pathogenic mold; pullulanases derived from Bacillus acidopullyticus; beta-galactosidases derived from Kluyveromyces fragllis; xylanases derived from Trichoderma reesei other examples include BAN ™, MaFtogenase ™, an alpha-amylase derived from Bacillus subtilis ·, an alpha-amylase derived from Bacillus amyioliquefaclens-, an alpha-amylase derived from Bacillus stearothermophilus; an α-amylase derived from Aspergillus oryzae] an alpha-amylase derived from a non-pathogenic microorganism, Fungamyl ™ or a CGTase, such as a CGTase derived from a strain of Bacillus autolyticus, a strain of Bacillus cereus, a strain of Bacillus circulars , a strain of

Bacillus circulans var. alkalophllus, a strain of Bacillus coagulans, a strain of Bacillus firmus, a »train of Bacillus halophllus, a strain of Bacillus macerans, a strain of Bacillus msgaterlum, a strain of Bacillus ohbensis, a strain of Bacillus stearothermophllus, a strain of Bacillus subtills, a strain of Klebsiella pneumoniae, a strain of Thermoanaerobader sp., a strain of Thermoanaerobader ethanollcus, a strain of Thermoanaerobader fmnil, a strain of Clostridium thermoamylolytlcum, a strain of Clostridium thermosaccharolyticum, or a strain of Thermoanaerobacterium thermosulfurigenes or a transglucosidase, such as a transglucosidase derived from Aspergillus nlger, a.g. the product sold by Amamo Pharmaceutical Co., Japan, Promozyme™ or a dextranase such as a dextranase derived from fungi of Penicillium, Paecilomycas, Aspergillus, Fusahum, Spicaria, Verticillium, Halminthosporium or the Chaetomium genera; bacteria of the genera Lactobacillus, Streptococcus, Cellvibrio, Cytophaga, Brevlbacterium, Pseudomonas, Corynabaderium, Arthrobader or Flavobaderium or yeasts such as Lipomyces starkeyi, an example being Dextranase 50 L produced by fermentation of strains of Penicillium lilaclum or a mutanase such as a mutanase derived from Trichoderma or from a strain of Streptomyces, Cladosporium reslnae, Pseudomonas sp., Flavobaderium sp., Bacillus circulansa or Aspergillus sp. or from Trichoderma harzianum, in particular from a strain of Trichoderma harzianum, such as Trichoderma harzianum CBS 243.71, or Penicillium, in particular a strain of Penicillium funiculosum, such as Penicillium funiculosum NRRL 1768, or a strain of Penicillium lilaclnum, such as Penicillium lilaclnum NRRL 896, or a strain of Penicillium purpumgenum, such as the strain of Penicillium purpurogenum CBS 238.95, or a strain of the genus Pseudomonas, or a strain of Flavobacterium sp., or a strain of Bacillus clrculanse or a strain of Aspergillus sp., or a strain of Streptomyces, Penicillium purpurogenum or an oxidoreductase or a laccase or related enzymes, such as laccases or related enzymes derived from a strain of Polypoms sp., in particular a strain of Polyporus pinsitus (also called Trametas villose) or Polyporus versicolor, or a strain of Myceliophthora sp., a.g. M. thermophila or a strain of Rhizodonia sp., in particular a strain of Rhizodonia praticola or Rhizodonia solani, or a strain of Scytalidium sp., in particular S. thermophitium, or a strain of Pyricularia sp., in particular Pyricularia oryzae, or a strain of Coprinus sp., such as a C. cinereus, or from a fungus such as Cdlybia, Fomes, Lentinus, Pleurotus, Aspergillus, Neurospora, Podospora, Phlebia, e.g. P. radiata, Coriolus sp., e.g. C. hirsltus, or Botrytis or a Bilirubin oxidase, such as a bilirubin oxidase derived from a strain of Myrothecium sp., such as a strain of M. vemicaria or a peroxidase such as peroxidase derived from a strain of the fungus species Coprinus, in particular a strain of Coprinus cinereus or Coprinus macrorhizus, or derived from a strain of the bacteria genus Bacillus, in particular a strain of Badllus pumilus or a haloperoxidase, such as a haloperoxidase derived from Curvularia sp., In particular C. verruculosa or an oxidase, such as an L-amino oxidase derived from a strain of Trichoderma sp, such as Trichoderma harzianum, such as the L-amino acid oxidase, or Trichoderma vinds or aglucose oxidase derived from a strain of Aspergillus sp., e.g. Aspergillus higer, Cladosporium sp., e.g. Cladosporium oxyspomm or a hexose oxidase derived from the red sea-weed Chondrus crispus (commonly known as Irish moss) the red sea-weed Iridophycus flaccidum or a xylitol oxidase derived from a strain of Streptomyæs sp. (e.g. Streptomyces IKD472. FERM Ρ-1433Θ). 9. Use of a composition according to any of the preceding claims in oral care for humans and/or animals. 10. A product comprising a composition according to any of claims 1-8. 11. Use of a product according to claim 10 in oral care for humans and/or animals.Bacillus circulans var. alkalophllus, a strain of Bacillus coagulans, a strain of Bacillus firmus, a »train of Bacillus halophllus, a strain of Bacillus macerans, a strain of Bacillus msgaterlum, a strain of Bacillus ohbensis, a strain of Bacillus stearothermophllus, a strain of Bacillus subtills , a strain of Klebsiella pneumoniae, a strain of Thermoanaerobader sp., a strain of Thermoanaerobader ethanollcus, a strain of Thermoanaerobader fmnil, a strain of Clostridium thermoamylolytlcum, a strain of Clostridium thermosaccharolyticum, or a strain of Thermoanaurobucerium aos, a transglucosidase derived from Aspergillus nlger, ag the product sold by Amamo Pharmaceutical Co., Japan, Promozyme ™ or a dextranase such as a dextranase derived from fungi of Penicillium, Paecilomycas, Aspergillus, Fusahum, Spicaria, Verticillium, Halminthosporium or the Chaetomium genera; bacteria of the genera Lactobacillus, Streptococcus, Cellvibrio, Cytophaga, Brevlbacterium, Pseudomonas, Corynabaderium, Arthrobader or Flavobaderium or yeasts such as Lipomyces starkeyi, an example being Dextranase 50 L produced by fermentation of strains of Penicillium mutanase such as a mutation. from Trichoderma or from a strain of Streptomyces, Cladosporium reslnae, Pseudomonas sp., Flavobaderium sp., Bacillus circulansa or Aspergillus sp. or from Trichoderma harzianum, in particular from a strain of Trichoderma harzianum, such as Trichoderma harzianum CBS 243.71, or Penicillium, in particular a strain of Penicillium funiculosum, such as Penicillium funiculosum NRRL 1768, or a strain of Penicillium lilaclnum, l as Penicillium lilaclnum, such as NRRL 896, or a strain of Penicillium purpumgenum, such as the strain of Penicillium purpurogenum CBS 238.95, or a strain of the genus Pseudomonas, or a strain of Flavobacterium sp., Or a strain of Bacillus clrculanse or a strain of Aspergillus sp., or a strain of Streptomyces, Penicillium purpurogenum or an oxidoreductase or a laccase or related enzymes, such as laccases or related enzymes derived from a strain of Polypoms sp., in particular a strain of Polyporus pinsitus (also called Trametas villose) or Polyporus versicolor, or a strain of Myceliophthora sp., ag M. thermophila or a strain of Rhizodonia sp., In particular a strain of Rhizodonia praticola or Rhizodonia solani, or a strain of Scytalidium sp., In particular S. thermophitium, or a strain of Pyricularia sp., In particular Pyricularia oryzae, or a strain of Coprinus sp., such as a C. cinereus, or from a fungus such as Cdlybia, Fomes, Lentinus, Pleurotus, Aspergillus, Neurospora, Podospora, Phlebia, eg P. radiata, Coriolus sp., E.g. C. hirsltus, or Botrytis or a Bilirubin oxidase, such as a bilirubin oxidase derived from a strain of Myrothecium sp., Such as a strain of M. vemicaria or a peroxidase such as peroxidase derived from a strain of the fungus species Coprinus, in particular a strain of Coprinus cinereus or Coprinus macrorhizus, or derived from a strain of the bacteria genus Bacillus, in particular a strain of Badllus pumilus or a haloperoxidase, such as a haloperoxidase derived from Curvularia sp., In particular C. verruculosa or an oxidase , such as an L-amino oxidase derived from a strain of Trichoderma sp, such as Trichoderma harzianum, such as the L-amino acid oxidase, or Trichoderma vinds or aglucose oxidase derived from a strain of Aspergillus sp., eg Aspergillus higer, Cladosporium sp., E.g. Cladosporium oxyspomm or a hexose oxidase derived from the red sea-weed Chondrus crispus (commonly known as Irish moss) the red sea-weed Iridophycus flaccidum or a xylitol oxidase derived from a strain of Streptomyæs sp. (e.g. Streptomyces IKD472. FERM Ρ-1433Θ). 9. Use of a composition according to any of the preceding claims in oral care for humans and / or animals. A product comprising a composition according to any of claims 1-8. 11. Use of a product according to claim 10 in oral care for humans and / or animals.

DK200101777A 2001-11-30 2001-11-30 Protease for oral care is substantially inactive in natural mouth environment when oral care composition is absent DK200101777A (en)

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