DK1869171T3 - Virus cleaning using ultrafiltration - Google Patents
Virus cleaning using ultrafiltrationInfo
- Publication number
- DK1869171T3 DK1869171T3 DK06724513T DK06724513T DK1869171T3 DK 1869171 T3 DK1869171 T3 DK 1869171T3 DK 06724513 T DK06724513 T DK 06724513T DK 06724513 T DK06724513 T DK 06724513T DK 1869171 T3 DK1869171 T3 DK 1869171T3
- Authority
- DK
- Denmark
- Prior art keywords
- ultrafiltration
- virus
- virus cleaning
- adenovirus
- cleaning
- Prior art date
Links
- 241000700605 Viruses Species 0.000 title abstract 2
- 238000000108 ultra-filtration Methods 0.000 title abstract 2
- 238000004140 cleaning Methods 0.000 title 1
- 241000701161 unidentified adenovirus Species 0.000 abstract 2
- 238000004113 cell culture Methods 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000012466 permeate Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000012465 retentate Substances 0.000 abstract 1
- 238000005199 ultracentrifugation Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10051—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
Described are methods for purification of recombinant adenovirus from cell culture using a step of ultrafiltration wherein the retentate contains the virus. By applying back pressure on the permeate side during this step, adenovirus can be obtained at high purity without the need for chromatography or ultracentrifugation steps.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US67006405P | 2005-04-11 | 2005-04-11 | |
EP05102842 | 2005-04-11 | ||
PCT/EP2006/003722 WO2006108707A1 (en) | 2005-04-11 | 2006-04-11 | Virus purification using ultrafiltration |
Publications (2)
Publication Number | Publication Date |
---|---|
DK1869171T3 true DK1869171T3 (en) | 2009-02-16 |
DK1869171T4 DK1869171T4 (en) | 2016-01-18 |
Family
ID=36572264
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK06724513.4T DK1869171T4 (en) | 2005-04-11 | 2006-04-11 | Virus cleaning using ultrafiltration |
Country Status (10)
Country | Link |
---|---|
US (1) | US8574595B2 (en) |
EP (1) | EP1869171B2 (en) |
CN (1) | CN101155915B (en) |
AT (1) | ATE412737T1 (en) |
AU (1) | AU2006233800B2 (en) |
CA (1) | CA2602944C (en) |
DE (1) | DE602006003420D1 (en) |
DK (1) | DK1869171T4 (en) |
ES (1) | ES2317517T5 (en) |
WO (1) | WO2006108707A1 (en) |
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-
2006
- 2006-04-11 CN CN2006800118153A patent/CN101155915B/en active Active
- 2006-04-11 CA CA2602944A patent/CA2602944C/en active Active
- 2006-04-11 EP EP06724513.4A patent/EP1869171B2/en active Active
- 2006-04-11 US US11/909,955 patent/US8574595B2/en active Active
- 2006-04-11 ES ES06724513.4T patent/ES2317517T5/en active Active
- 2006-04-11 AU AU2006233800A patent/AU2006233800B2/en active Active
- 2006-04-11 DK DK06724513.4T patent/DK1869171T4/en active
- 2006-04-11 DE DE602006003420T patent/DE602006003420D1/en active Active
- 2006-04-11 WO PCT/EP2006/003722 patent/WO2006108707A1/en not_active Application Discontinuation
- 2006-04-11 AT AT06724513T patent/ATE412737T1/en active
Also Published As
Publication number | Publication date |
---|---|
WO2006108707A1 (en) | 2006-10-19 |
CN101155915B (en) | 2013-12-04 |
US20090123989A1 (en) | 2009-05-14 |
EP1869171B1 (en) | 2008-10-29 |
ES2317517T5 (en) | 2016-01-21 |
CA2602944C (en) | 2015-08-11 |
DE602006003420D1 (en) | 2008-12-11 |
EP1869171B2 (en) | 2015-10-14 |
ATE412737T1 (en) | 2008-11-15 |
ES2317517T3 (en) | 2009-04-16 |
CN101155915A (en) | 2008-04-02 |
AU2006233800A1 (en) | 2006-10-19 |
US8574595B2 (en) | 2013-11-05 |
CA2602944A1 (en) | 2006-10-19 |
AU2006233800B2 (en) | 2011-07-07 |
DK1869171T4 (en) | 2016-01-18 |
EP1869171A1 (en) | 2007-12-26 |
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