DK1654352T3 - Reversibly immortalized olfactory enveloping glia and their use in promoting neuronal regeneration - Google Patents

Reversibly immortalized olfactory enveloping glia and their use in promoting neuronal regeneration

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Publication number
DK1654352T3
DK1654352T3 DK04743484T DK04743484T DK1654352T3 DK 1654352 T3 DK1654352 T3 DK 1654352T3 DK 04743484 T DK04743484 T DK 04743484T DK 04743484 T DK04743484 T DK 04743484T DK 1654352 T3 DK1654352 T3 DK 1654352T3
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Prior art keywords
capacity
cell lines
cns
glia
oegs
Prior art date
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DK04743484T
Other languages
Danish (da)
Inventor
Flores Maria Moreno
Bermejo Maria J Martin
De Grado Avila
Jurado Wandosell
Nido Javier Diaz
Filip Lim
Izquierdo Erika Pastrana
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Univ Madrid Autonoma
Consejo Superior Investigacion
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Application filed by Univ Madrid Autonoma, Consejo Superior Investigacion filed Critical Univ Madrid Autonoma
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Publication of DK1654352T3 publication Critical patent/DK1654352T3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/062—Sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/30—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cancer cells, e.g. reversion of tumour cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Neurosurgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Acoustics & Sound (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

The present invention is based on the capacity of the Olfactory Ensheathing Glia (OEG) to foster axonal regeneration in the adult mammalian central nervous system (CNS). This specific capacity is probably due to a combination of several factors, such as the molecular composition of cellular membrane and/or the capacity to secrete some molecules; combined with the capacity to reduce glial scar and accompany new growing axon in the damaged CNS. We have developed immortalised cell lines from primary human OEGs. The cells were cultured from post-mortem human tissue from donors and immortalised using a reversible system. Some of these OEG human clonal cell lines were selected by their ability to promote axonal regeneration from adult rat retinal ganglion neurons in a similar fashion to primary OEGs. These cell lines, alone or in pharmaceutical compositions comprising these cells, may be used to repair neuronal damage in the CNS.
DK04743484T 2003-07-18 2004-07-19 Reversibly immortalized olfactory enveloping glia and their use in promoting neuronal regeneration DK1654352T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0316882.0A GB0316882D0 (en) 2003-07-18 2003-07-18 Reversible immortalization of OEG from human olfactory bulbs as a tool to promote spinal cord regeneration
PCT/GB2004/003149 WO2005012513A1 (en) 2003-07-18 2004-07-19 Reversibly immortalised olfactory ensheathing glia and their use to promote neuronal regenaration

Publications (1)

Publication Number Publication Date
DK1654352T3 true DK1654352T3 (en) 2008-07-07

Family

ID=27764099

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04743484T DK1654352T3 (en) 2003-07-18 2004-07-19 Reversibly immortalized olfactory enveloping glia and their use in promoting neuronal regeneration

Country Status (17)

Country Link
US (1) US20070134213A1 (en)
EP (1) EP1654352B1 (en)
JP (1) JP2007536901A (en)
KR (1) KR100985191B1 (en)
CN (1) CN100554410C (en)
AT (1) ATE384131T1 (en)
AU (1) AU2004261763B8 (en)
CA (1) CA2531320A1 (en)
DE (1) DE602004011361T2 (en)
DK (1) DK1654352T3 (en)
ES (1) ES2299842T3 (en)
GB (1) GB0316882D0 (en)
HR (1) HRP20080051T3 (en)
IL (1) IL172831A (en)
PL (1) PL1654352T3 (en)
PT (1) PT1654352E (en)
WO (1) WO2005012513A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL212052B1 (en) * 2005-12-14 2012-08-31 Akademia Medyczna Im Piastow Śląskich We Wrocławiu Method for the acquirement of gley smell cells and their applications
DK3257943T3 (en) 2010-11-02 2019-10-21 Helmholtz Zentrum Infektionsforschung Gmbh METHODS AND VECTORS FOR CELL IMMORTALIZATION
WO2012164137A1 (en) 2011-05-30 2012-12-06 Fundación Investigación En Regeneración Del Sistema Nervioso Stem cells and neural crest cells derived from olfactory ensheathing glia, and uses thereof
KR102102837B1 (en) * 2015-12-04 2020-04-22 더 펜 스테이트 리서어치 파운데이션 Chemical reprogramming of human glial cells into neurons using small molecule mixtures
CN108277201A (en) * 2016-12-30 2018-07-13 中国医药大学 At body multifunctional Olfactory stem cell and its separation method and its application

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5629159A (en) * 1995-06-07 1997-05-13 California Institute Of Technology Immortalization and disimmortalization of cells
AUPQ369599A0 (en) * 1999-10-27 1999-11-18 Griffith University A method of preparing olfactory cells for transplantation
AUPQ614200A0 (en) * 2000-03-10 2000-03-30 Department Of Human Physiology, Flinders University School Of Medicine Method for treating chronic spinal cord injury
US20080233642A9 (en) * 2001-03-16 2008-09-25 Naoya Kobayashi Mammalian immortalized liver cell
WO2002074968A1 (en) * 2001-03-16 2002-09-26 Naoya Kobayashi Method for proliferating a liver cell, a liver cell obtained thereby, and use thereof
ES2190336B1 (en) * 2001-04-19 2005-02-01 Consejo Superior De Investigaciones Cientificas GENETICALLY MODIFIED MODIFIED OLFATORY GLIA BY INTRODUCTION OF TELOMERASE.
US6645763B2 (en) * 2001-10-12 2003-11-11 Naoya Kobayashi Immortalized bone marrow mesenchymal stem cell

Also Published As

Publication number Publication date
HRP20080051T3 (en) 2008-07-31
JP2007536901A (en) 2007-12-20
CA2531320A1 (en) 2005-02-10
AU2004261763A1 (en) 2005-02-10
IL172831A0 (en) 2006-06-11
PT1654352E (en) 2008-04-21
DE602004011361T2 (en) 2008-10-23
EP1654352A1 (en) 2006-05-10
WO2005012513A8 (en) 2006-03-02
AU2004261763B2 (en) 2010-01-28
AU2004261763B8 (en) 2011-01-06
ATE384131T1 (en) 2008-02-15
EP1654352B1 (en) 2008-01-16
KR20060091293A (en) 2006-08-18
ES2299842T3 (en) 2008-06-01
KR100985191B1 (en) 2010-10-05
US20070134213A1 (en) 2007-06-14
DE602004011361D1 (en) 2008-03-06
GB0316882D0 (en) 2003-08-20
IL172831A (en) 2011-05-31
PL1654352T3 (en) 2008-06-30
WO2005012513A1 (en) 2005-02-10
CN1823161A (en) 2006-08-23
CN100554410C (en) 2009-10-28

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