DK1200561T3 - Recombinant stable cell clone, its preparation and use - Google Patents

Recombinant stable cell clone, its preparation and use

Info

Publication number
DK1200561T3
DK1200561T3 DK99939251T DK99939251T DK1200561T3 DK 1200561 T3 DK1200561 T3 DK 1200561T3 DK 99939251 T DK99939251 T DK 99939251T DK 99939251 T DK99939251 T DK 99939251T DK 1200561 T3 DK1200561 T3 DK 1200561T3
Authority
DK
Denmark
Prior art keywords
cell clone
stabile
proteins
recombinant
preparation
Prior art date
Application number
DK99939251T
Other languages
Danish (da)
Inventor
Wolfgang Mundt
Friedrich Dorner
Manfred Reiter
Original Assignee
Baxter Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=3683861&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1200561(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Baxter Ag filed Critical Baxter Ag
Application granted granted Critical
Publication of DK1200561T3 publication Critical patent/DK1200561T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/76—Undefined extracts from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to a stabile recombinant cell clone which is stabile in a medium containing no serum and proteins for at least 40 generations, and to a biomass which is obtained by multiplying the stabile cell clone under cultivation conditions that do not involve the use of serum or proteins. The invention also relates to a method for producing recombinant proteins using the biomass, to a method for producing stabile recombinant cell clones, and to the production of a recombinant protein in a synthetic minimal medium that does contain serum or proteins.
DK99939251T 1999-08-05 1999-08-05 Recombinant stable cell clone, its preparation and use DK1200561T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/AT1999/000197 WO2001011021A1 (en) 1999-08-05 1999-08-05 Recombinant stabile cell clone, its production and use thereof

Publications (1)

Publication Number Publication Date
DK1200561T3 true DK1200561T3 (en) 2006-10-16

Family

ID=3683861

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99939251T DK1200561T3 (en) 1999-08-05 1999-08-05 Recombinant stable cell clone, its preparation and use

Country Status (11)

Country Link
EP (1) EP1200561B1 (en)
JP (1) JP2003506077A (en)
AT (1) ATE330003T1 (en)
AU (1) AU780810B2 (en)
CA (1) CA2381173A1 (en)
CY (1) CY1106111T1 (en)
DE (1) DE59913565D1 (en)
DK (1) DK1200561T3 (en)
ES (1) ES2267280T3 (en)
PT (1) PT1200561E (en)
WO (1) WO2001011021A1 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2243827B2 (en) 1996-08-30 2017-11-22 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
AT409379B (en) 1999-06-02 2002-07-25 Baxter Ag MEDIUM FOR PROTEIN- AND SERUM-FREE CELL CULTURE
HUP0301245A3 (en) * 2000-10-02 2005-12-28 Novo Nordisk Healthcare Ag Tmethod for the production of vitamin k-dependent proteins
KR20040039472A (en) 2001-10-02 2004-05-10 노보 노르디스크 헬스 케어 악티엔게젤샤프트 Method for production of recombinant proteins in eukaryote cells
HUP0402226A2 (en) * 2001-11-28 2005-02-28 Sandoz Ag. Cell culture process
US20050123532A1 (en) * 2001-12-04 2005-06-09 Takaharu Kouno Method of activating protein
EP1520008B1 (en) 2002-07-09 2012-09-05 Baxter International Inc. Animal protein free media for cultivation of cells
DE10338531A1 (en) * 2003-08-19 2005-04-07 Boehringer Ingelheim Pharma Gmbh & Co. Kg Method for recloning production cells
US8669109B2 (en) 2003-08-19 2014-03-11 Boehringer Ingelheim Pharma Gmbh & Co. Kg Methods of producing proteins in Chinese hamster ovary (CHO) cells
BRPI0417916A (en) * 2003-12-31 2007-04-10 Merck Patent Gmbh fc-erythropoietin fusion protein with improved pharmacokinetics
AU2011221414B2 (en) * 2004-10-29 2012-09-20 Takeda Pharmaceutical Company Limited Animal Protein-Free Media for Cultivation of Cells
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
ATE541919T1 (en) 2005-02-11 2012-02-15 Novo Nordisk Healthcare Ag PRODUCTION OF A PROTEIN IN SERUM-FREE CELL CULTURE CONTAINING A PROTEIN HYDROLYZATE FROM PLANTS
ES2474573T3 (en) 2006-01-04 2014-07-09 Baxter International Inc Cell culture medium without oligopeptides
EP2977461A1 (en) * 2008-11-12 2016-01-27 Baxalta Incorporated Method of producing serum-free insulin-free factor vii
WO2024024671A1 (en) * 2022-07-27 2024-02-01 不二製油グループ本社株式会社 Animal cell proliferation promoter

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
DE4313620A1 (en) * 1993-04-26 1994-10-27 Biotechnolog Forschung Gmbh Hamster cell lines and methods for glycoprotein recovery
EP0666312A1 (en) * 1994-02-08 1995-08-09 Wolfgang A. Renner Process for the improvement of mammalian cell growth
EP1213030B1 (en) * 1994-11-10 2009-04-22 Baxter Healthcare SA Method for producing biologicals in protein-free culture
AU4302796A (en) * 1994-12-16 1996-07-03 Novartis Ag Production of recombinant secretory component
AUPN442295A0 (en) * 1995-07-26 1995-08-17 Commonwealth Scientific And Industrial Research Organisation Regulated autocrine growth of mammalian cells
US5851800A (en) * 1996-05-14 1998-12-22 Pharmacia & Upjohn Ab Process for producing a protein

Also Published As

Publication number Publication date
PT1200561E (en) 2006-09-29
EP1200561A1 (en) 2002-05-02
AU5363699A (en) 2001-03-05
DE59913565D1 (en) 2006-07-27
ES2267280T3 (en) 2007-03-01
ATE330003T1 (en) 2006-07-15
JP2003506077A (en) 2003-02-18
CY1106111T1 (en) 2011-06-08
CA2381173A1 (en) 2001-02-15
WO2001011021A1 (en) 2001-02-15
AU780810B2 (en) 2005-04-21
EP1200561B1 (en) 2006-06-14

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