DK104990D0 - GLUCURONIDE PERMEASE CODING GENE AND ITS USE, AND RECOMBINANT DNA MOLECULE AND HOST CELL TRANSFORMANT CONTAINING IT - Google Patents

GLUCURONIDE PERMEASE CODING GENE AND ITS USE, AND RECOMBINANT DNA MOLECULE AND HOST CELL TRANSFORMANT CONTAINING IT

Info

Publication number
DK104990D0
DK104990D0 DK104990A DK104990A DK104990D0 DK 104990 D0 DK104990 D0 DK 104990D0 DK 104990 A DK104990 A DK 104990A DK 104990 A DK104990 A DK 104990A DK 104990 D0 DK104990 D0 DK 104990D0
Authority
DK
Denmark
Prior art keywords
glucuronide permease
glucuronide
beta
host cell
recombinant dna
Prior art date
Application number
DK104990A
Other languages
Danish (da)
Other versions
DK173224B1 (en
DK104990A (en
Inventor
Richard Anthony Jefferson
Original Assignee
Richard Anthony Jefferson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Richard Anthony Jefferson filed Critical Richard Anthony Jefferson
Publication of DK104990D0 publication Critical patent/DK104990D0/en
Publication of DK104990A publication Critical patent/DK104990A/en
Application granted granted Critical
Publication of DK173224B1 publication Critical patent/DK173224B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01031—Beta-glucuronidase (3.2.1.31)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to the gene encoding the transport protein, glucuronide permease. Recombinant vectors encoding glucuronide permease can be used to transfect host cells. Expression of glucuronide permease by the transformants allow cellular uptake of beta -glucuronides. This system permits the detection of beta -glucuronidase activity in vivo, and can conveniently be used together with GUS gene fusions. In addition to providing an important complement to the GUS system, glucuronide permease also can be used in a method to selectively alter the permeability of cells. Because of the variety of substances which can be conjugated to beta -glucuronides, glucuronide permease provides a cellular entrance for a multitude of compounds.
DK199001049A 1987-10-29 1990-04-27 Recombinant DNA molecule encoding glucuronide permease, host cell transformant containing the DNA molecule and pharmaceutical DK173224B1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB8725402 1987-10-29
GB878725402A GB8725402D0 (en) 1987-10-29 1987-10-29 Gene fusion
PCT/GB1988/000936 WO1989003880A1 (en) 1987-10-29 1988-10-31 A gene coding for glucuronide permease
GB8800936 1988-10-31

Publications (3)

Publication Number Publication Date
DK104990D0 true DK104990D0 (en) 1990-04-27
DK104990A DK104990A (en) 1990-06-21
DK173224B1 DK173224B1 (en) 2000-04-17

Family

ID=10626142

Family Applications (1)

Application Number Title Priority Date Filing Date
DK199001049A DK173224B1 (en) 1987-10-29 1990-04-27 Recombinant DNA molecule encoding glucuronide permease, host cell transformant containing the DNA molecule and pharmaceutical

Country Status (7)

Country Link
EP (1) EP0383808B1 (en)
JP (1) JPH03502042A (en)
AT (1) ATE123522T1 (en)
DE (1) DE3853955T2 (en)
DK (1) DK173224B1 (en)
GB (2) GB8725402D0 (en)
WO (1) WO1989003880A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK152291D0 (en) * 1991-08-28 1991-08-28 Danisco PROCEDURE AND CHEMICAL RELATIONS
IL108499A (en) * 1993-02-04 2000-02-29 Lilly Co Eli Mammalian influx peptide transporter
US7087420B1 (en) 1997-07-17 2006-08-08 Cambia Microbial β-glucuronidase genes, gene products and uses thereof
US6641996B1 (en) * 1997-09-09 2003-11-04 Cambia Microbial β-glucuronidase genes, gene products and uses thereof
US6391547B1 (en) 1997-09-09 2002-05-21 Center For The Application Of Molecular Biology To International Agriculture Microbial β-glucuronidase genes, gene products and uses thereof
WO2007134851A1 (en) 2006-05-24 2007-11-29 Saudi Basic Industries Corporation Process for preparing a catalyst component for propylene polymerization

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8626862D0 (en) * 1986-11-11 1986-12-10 Jefferson R A Gene fusion

Also Published As

Publication number Publication date
GB2229184A (en) 1990-09-19
DE3853955D1 (en) 1995-07-13
EP0383808B1 (en) 1995-06-07
ATE123522T1 (en) 1995-06-15
EP0383808A1 (en) 1990-08-29
DK173224B1 (en) 2000-04-17
GB2229184B (en) 1992-01-22
JPH03502042A (en) 1991-05-16
GB8725402D0 (en) 1987-12-02
DK104990A (en) 1990-06-21
GB9009522D0 (en) 1990-07-04
WO1989003880A1 (en) 1989-05-05
DE3853955T2 (en) 1996-02-08

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Legal Events

Date Code Title Description
B1 Patent granted (law 1993)
PBP Patent lapsed

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