DK102782A - PROCEDURE FOR IMMOBILIZATION OF GLUCO SEISOMERASE ACTIVE MICROORGANISMS - Google Patents

PROCEDURE FOR IMMOBILIZATION OF GLUCO SEISOMERASE ACTIVE MICROORGANISMS Download PDF

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Publication number
DK102782A
DK102782A DK102782A DK102782A DK102782A DK 102782 A DK102782 A DK 102782A DK 102782 A DK102782 A DK 102782A DK 102782 A DK102782 A DK 102782A DK 102782 A DK102782 A DK 102782A
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Prior art keywords
glucose
immobilized
seisomerase
gluco
immobilization
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DK102782A
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Danish (da)
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DK151272C (en
DK151272B (en
Inventor
Mitko Stoychev Popov
Fidanko Stiliyanov Sretenov
Rossen Haralanov Rachev
Galina Mihaylova Djejeva
Sebastiya A Valcheva Bojkova
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Inst Microbiologia
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Priority to DK102782A priority Critical patent/DK151272C/en
Publication of DK102782A publication Critical patent/DK102782A/en
Publication of DK151272B publication Critical patent/DK151272B/en
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Publication of DK151272C publication Critical patent/DK151272C/en

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  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Microorganisms that possess a glucose isomerase activity are immobilized on a protein-containing support or carrier, which is either blood serum or blood plasma. The cells are mixed with fresh, spray-dried, freeze-dried or frozen serum or plasma in a weight-to-volume ratio of between 1 : 1 and 1 : 10, after which they are immobilized by the addition of glutaraldehyde. The material treated in this way can be used for converting glucose to fructose, e.g. by passing a 2-3 M glucose syrup continuously through a column containing the immobilized cells. The supply of the substrate is preferably regulated in such a way that 40-50% of the glucose is converted into fructose. The preparation obtained by the process according to the invention has a high enzyme activity, a favourable pH optimum, a high temperature optimum and a good thermal stability.
DK102782A 1982-03-09 1982-03-09 PROCEDURE FOR IMMOBILIZING GLUCOSE ISOMERASE ACTIVE MICROORGANISMS. DK151272C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK102782A DK151272C (en) 1982-03-09 1982-03-09 PROCEDURE FOR IMMOBILIZING GLUCOSE ISOMERASE ACTIVE MICROORGANISMS.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK102782 1982-03-09
DK102782A DK151272C (en) 1982-03-09 1982-03-09 PROCEDURE FOR IMMOBILIZING GLUCOSE ISOMERASE ACTIVE MICROORGANISMS.

Publications (3)

Publication Number Publication Date
DK102782A true DK102782A (en) 1983-09-10
DK151272B DK151272B (en) 1987-11-16
DK151272C DK151272C (en) 1988-07-04

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DK102782A DK151272C (en) 1982-03-09 1982-03-09 PROCEDURE FOR IMMOBILIZING GLUCOSE ISOMERASE ACTIVE MICROORGANISMS.

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DK151272C (en) 1988-07-04
DK151272B (en) 1987-11-16

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