DK0970249T3 - Method for Detecting Mutant Alleles - Google Patents

Method for Detecting Mutant Alleles

Info

Publication number
DK0970249T3
DK0970249T3 DK98919054T DK98919054T DK0970249T3 DK 0970249 T3 DK0970249 T3 DK 0970249T3 DK 98919054 T DK98919054 T DK 98919054T DK 98919054 T DK98919054 T DK 98919054T DK 0970249 T3 DK0970249 T3 DK 0970249T3
Authority
DK
Denmark
Prior art keywords
allels
mutant alleles
detecting mutant
wild type
detecting
Prior art date
Application number
DK98919054T
Other languages
Danish (da)
Inventor
Christoph Wagener
Original Assignee
Christoph Wagener
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Christoph Wagener filed Critical Christoph Wagener
Application granted granted Critical
Publication of DK0970249T3 publication Critical patent/DK0970249T3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel

Abstract

The invention relates to a method for detecting mutated allels in an excess of wild type allels. According to said method, said wild type allels are isolated by means of a separation process using a carrier which is bonded to one or several oligonucleotides, said oligonucleotides being complementary to the wild type allels.
DK98919054T 1997-03-04 1998-03-04 Method for Detecting Mutant Alleles DK0970249T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19708758A DE19708758A1 (en) 1997-03-04 1997-03-04 Procedure for the detection of mutant alleles
PCT/DE1998/000676 WO1998039472A2 (en) 1997-03-04 1998-03-04 Method for detecting mutated allels

Publications (1)

Publication Number Publication Date
DK0970249T3 true DK0970249T3 (en) 2003-03-17

Family

ID=7822190

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98919054T DK0970249T3 (en) 1997-03-04 1998-03-04 Method for Detecting Mutant Alleles

Country Status (9)

Country Link
EP (1) EP0970249B1 (en)
JP (1) JP2001514504A (en)
AT (1) ATE228573T1 (en)
AU (1) AU7204298A (en)
CA (1) CA2283188A1 (en)
DE (2) DE19708758A1 (en)
DK (1) DK0970249T3 (en)
ES (1) ES2187964T3 (en)
WO (1) WO1998039472A2 (en)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU632494B2 (en) * 1988-05-20 1993-01-07 F. Hoffmann-La Roche Ag Immobilized sequence-specific probes
US5352578A (en) * 1989-02-15 1994-10-04 Worcester Foundation For Experimental Biology Method of separating oligonucleotides from a mixture
JPH0430800A (en) * 1990-05-28 1992-02-03 Tosoh Corp Detection of mutation
DE69121791T2 (en) * 1990-06-08 1997-01-23 Behringwerke Ag Procedure for the quantitative determination of DNA sequences
WO1993022457A1 (en) * 1992-04-24 1993-11-11 Massachusetts Institute Of Technology Screening for genetic variation
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
US5512441A (en) * 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method

Also Published As

Publication number Publication date
DE19708758A1 (en) 1998-09-17
AU7204298A (en) 1998-09-22
CA2283188A1 (en) 1998-09-11
ATE228573T1 (en) 2002-12-15
DE59806443D1 (en) 2003-01-09
EP0970249B1 (en) 2002-11-27
JP2001514504A (en) 2001-09-11
WO1998039472A2 (en) 1998-09-11
EP0970249A2 (en) 2000-01-12
WO1998039472A3 (en) 1998-12-10
ES2187964T3 (en) 2003-06-16

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