DK0487619T3 - Process for obtaining biologically active peptide-enriched fractions from beta-casein and peptide fractions obtained by the method - Google Patents

Process for obtaining biologically active peptide-enriched fractions from beta-casein and peptide fractions obtained by the method

Info

Publication number
DK0487619T3
DK0487619T3 DK90913211.0T DK90913211T DK0487619T3 DK 0487619 T3 DK0487619 T3 DK 0487619T3 DK 90913211 T DK90913211 T DK 90913211T DK 0487619 T3 DK0487619 T3 DK 0487619T3
Authority
DK
Denmark
Prior art keywords
casein
peptide
carried out
fractions
precursors
Prior art date
Application number
DK90913211.0T
Other languages
Danish (da)
Inventor
Jean Louis Maubois
Joelle Leonil
Francoise Nau
Daniel Molle
Original Assignee
Agronomique Inst Nat Rech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agronomique Inst Nat Rech filed Critical Agronomique Inst Nat Rech
Application granted granted Critical
Publication of DK0487619T3 publication Critical patent/DK0487619T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4732—Casein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

In order to obtain, from casein $g(b), a partial hydrolyzate enriched with, firstly, a phosphopeptide or a 1-25 peptide fraction, and, secondly, precursors of $g(b)-casomorphine (these precursors being fragments which cover the 33-97 region of the casein $g(b) molecule), by hydrolyzing the casein $g(b) with a proteolytic enzyme, the hydrolysis is carried out in a saline medium, thereby causing a precipitate to appear in the medium and separating it from the soluble phase which represents the partial hydrolyzate desired. Preferably, this hydrolysis is carried out in a medium of which the ionic strength is equivalent to that of a solution of monovalent salt having an ionic strength of between 0.5 and 4M. In order to obtain the 1-25 peptide fraction and the precursors of $g(b)-casomorphine, ion exchange chromatography is carried out on this soluble phase, after having demineralized it by electrodialysis. In order to obtain the antihypertensive and immunostimulatory 177-183 peptide fraction, the precipitate obtained is made soluble, the resulting solution is brought to a pH level of between 6 and 9, and hydrolysis is carried out by means of a proteolytic enzyme.
DK90913211.0T 1989-08-16 1990-08-16 Process for obtaining biologically active peptide-enriched fractions from beta-casein and peptide fractions obtained by the method DK0487619T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8910931A FR2650955B1 (en) 1989-08-16 1989-08-16 PROCESS FOR OBTAINING, FROM THE BETA CASE, ENRICHED FRACTIONS IN BIOLOGICALLY ACTIVE PEPTIDES AND THE PEPTIDE FRACTIONS OBTAINED

Publications (1)

Publication Number Publication Date
DK0487619T3 true DK0487619T3 (en) 1995-03-13

Family

ID=9384733

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90913211.0T DK0487619T3 (en) 1989-08-16 1990-08-16 Process for obtaining biologically active peptide-enriched fractions from beta-casein and peptide fractions obtained by the method

Country Status (7)

Country Link
EP (1) EP0487619B1 (en)
AT (1) ATE112683T1 (en)
DE (1) DE69013350T2 (en)
DK (1) DK0487619T3 (en)
ES (1) ES2064759T3 (en)
FR (1) FR2650955B1 (en)
WO (1) WO1991002539A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE194623T1 (en) * 1991-04-19 2000-07-15 Univ Melbourne PRODUCTION OF PHOSPHOPEPTIDES FROM CASEIN
JPH08501688A (en) * 1992-09-22 1996-02-27 ニュージーランド デアリー リサーチ インスティテュート Method for manufacturing beta-casein fortified products
JP3782837B2 (en) * 1995-04-10 2006-06-07 カルピス株式会社 Antihypertensive agent and method for producing the same
IT1277964B1 (en) * 1995-12-27 1997-11-12 Biosistema Di Pier Luigi Spara PRODUCT DERIVED FROM MILK, SUBSTANTIALLY FREE OF NON-HUMAN MAMMALIAN BETACASEIN AND ITS USE
KR20020023425A (en) * 1999-08-24 2002-03-28 이이누마 카즈하루 Growth promoting compositions for mammals and method of promoting the growth of mammals by using the same
FR2841473B1 (en) * 2002-06-27 2004-09-17 Ingredia USE OF AT LEAST ONE PEPTIDE OF CASE S2 WITH INHIBITORY ACTIVITY OF THE ENGIOTENSIN I CONVERSION ENZYME FOR THE PREPARATION OF DRUGS, FOODS AND FOOD SUPPLEMENTS
US9055752B2 (en) 2008-11-06 2015-06-16 Intercontinental Great Brands Llc Shelf-stable concentrated dairy liquids and methods of forming thereof
UA112972C2 (en) 2010-09-08 2016-11-25 Інтерконтінентал Грейт Брендс ЛЛС LIQUID DAIRY CONCENTRATE WITH A HIGH CONTENT OF DRY SUBSTANCES

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2474828B1 (en) * 1980-02-01 1983-09-09 Agronomique Inst Nat Rech PROCESS FOR TREATING A CASEIN RAW MATERIAL CONTAINING BIVALENT CATION PHOSPHOCASEINATES, PRODUCTS OBTAINED AND APPLICATION
JPS59159793A (en) * 1983-02-28 1984-09-10 Meiji Seika Kaisha Ltd Purification of casein phosphopeptide
JPS62270533A (en) * 1986-05-20 1987-11-24 Agency Of Ind Science & Technol Peroral ingestible substance

Also Published As

Publication number Publication date
FR2650955B1 (en) 1992-01-10
ES2064759T3 (en) 1995-02-01
FR2650955A1 (en) 1991-02-22
DE69013350D1 (en) 1994-11-17
EP0487619B1 (en) 1994-10-12
DE69013350T2 (en) 1995-03-23
WO1991002539A1 (en) 1991-03-07
EP0487619A1 (en) 1992-06-03
ATE112683T1 (en) 1994-10-15

Similar Documents

Publication Publication Date Title
DE69029657D1 (en) HUMAN CYTOKIN, INTERLEUKIN-9
CA2099138A1 (en) Chondroitinase, Process for Preparing the Same, and Pharmaceutical Composition Comprising the Same
KR880700076A (en) Method for producing antigens of lymphocytic and acquired immune deficiency virus and methods of producing antibodies to the antigen
DK0588841T3 (en) Process for preparing a whey protein hydrolyzate
ES8104412A1 (en) Process for the preparation of a purified protein hydrolysate
DE69112611T2 (en) PROTEIN HYDROLYSATE.
DK0487619T3 (en) Process for obtaining biologically active peptide-enriched fractions from beta-casein and peptide fractions obtained by the method
DE69939268D1 (en) METHODS FOR CLEANING RECOMBINATIVE HUMAN ERYTHROPOIETIN FROM CELL CULTURAL RESEARCH
JP2622686B2 (en) Method for producing k-casein glycomacropeptide
PT97574A (en) PROCESS OF OBTAINING VARIABLE CD44 SURFACE PROTEINS, DNA SEQUENCES THAT CODE THEM, ANTIBODIES OF THESE PROTEINS, AND THEIR USE IN DIAGNOSIS AND THERAPY
PT98805A (en) A method of preparing a novel binding protein to the insulin-like growth factor (IGFBP-5)
ATE142694T1 (en) NEW METHOD FOR PRODUCING NON-FUSIONED PROTEIN IN E. COLI
FI953525A (en) Chemical synthesis procedure results in the preparation of a protein or polypeptide
AU580350B2 (en) Process for synthesising peptides
SU1487817A3 (en) Method of extracting carboxypeptidase b from adrenal gland of mammals
El-Negoumy Separation of lambda casein and some of its properties
SU411867A1 (en)
ABDEL-FATTAH et al. Purification and proteolytic action of milk-clotting enzyme produced by Penicillium citrinum
NO992531L (en) Methods of producing recombinant protein
DE59914155D1 (en) NEW PEPTIDE FRAGMENTS FOR CLEANING PROTEINS
SU1589210A1 (en) Method of extracting fat from liquid containing milk fat
Arav et al. Purification and characterization of acetyl esterase from bull testes
ATE28388T1 (en) PROTEOLYSIS OF PLASMA PROTEINS AND PRODUCTS OBTAINED FROM THE PROCESS.
DE3688974D1 (en) EXPRESSION SYSTEMS FOR THE PRODUCTION OF DESIRED PROTEINS.
ATE102950T1 (en) PROCESS FOR THE PREPARATION OF DIPEPTIDES WITH C-TERMINAL NON-PROTEINOGENIC AMINO ACIDS.