DK0463043T3 - Method for Stabilizing the Hybridization of Complementary Polynucleotide Sequences - Google Patents

Method for Stabilizing the Hybridization of Complementary Polynucleotide Sequences

Info

Publication number
DK0463043T3
DK0463043T3 DK90904868.8T DK90904868T DK0463043T3 DK 0463043 T3 DK0463043 T3 DK 0463043T3 DK 90904868 T DK90904868 T DK 90904868T DK 0463043 T3 DK0463043 T3 DK 0463043T3
Authority
DK
Denmark
Prior art keywords
hybridization
stabilizing
sequence
polynucleotide sequences
complementary polynucleotide
Prior art date
Application number
DK90904868.8T
Other languages
Danish (da)
Inventor
Benoit Prieur
Original Assignee
Opale Biotechnology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Opale Biotechnology filed Critical Opale Biotechnology
Application granted granted Critical
Publication of DK0463043T3 publication Critical patent/DK0463043T3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide

Abstract

The invention relates to a method for stabilizing the hybridization of a first DNA or RNA polynucleotide sequence, modified or not, with an anomeric configuration $g(a) or $g(b) by means of a second target sequence complementary of the first DNA or RNA sequence, characterized in that a peptide optionally fixed to said first sequence is complexed on the double hybridization helix formed by said first and second nucleotide sequences. The present invention also relates to peptide-polynucleotide conjugates useful for the method, as well as their application as diagnostic probes or as therapeutic agents, in particular.
DK90904868.8T 1989-03-14 1990-03-13 Method for Stabilizing the Hybridization of Complementary Polynucleotide Sequences DK0463043T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8903303A FR2644461B1 (en) 1989-03-14 1989-03-14 PROCESS FOR STABILIZING THE HYBRIDIZATION OF COMPLEMENTARY POLYNUCLEOTIDE SEQUENCES, PEPTIDE-POLYNUCLEOTIDE CONJUGATES USEFUL FOR THE IMPLEMENTATION OF THE PROCESS

Publications (1)

Publication Number Publication Date
DK0463043T3 true DK0463043T3 (en) 1994-10-10

Family

ID=9379652

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90904868.8T DK0463043T3 (en) 1989-03-14 1990-03-13 Method for Stabilizing the Hybridization of Complementary Polynucleotide Sequences

Country Status (10)

Country Link
EP (1) EP0463043B1 (en)
JP (1) JPH04506450A (en)
AT (1) ATE105871T1 (en)
AU (1) AU5337690A (en)
CA (1) CA2050913A1 (en)
DE (1) DE69009050T2 (en)
DK (1) DK0463043T3 (en)
ES (1) ES2056458T3 (en)
FR (1) FR2644461B1 (en)
WO (1) WO1990010713A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3945493A (en) * 1992-04-06 1993-11-08 University Of Medicine And Dentistry Of New Jersey Paired-ion oligonucleotides and methods for preparing same
US5556752A (en) * 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA

Also Published As

Publication number Publication date
EP0463043A1 (en) 1992-01-02
ES2056458T3 (en) 1994-10-01
CA2050913A1 (en) 1990-09-15
AU5337690A (en) 1990-10-09
DE69009050D1 (en) 1994-06-23
ATE105871T1 (en) 1994-06-15
FR2644461A1 (en) 1990-09-21
EP0463043B1 (en) 1994-05-18
DE69009050T2 (en) 1995-01-05
FR2644461B1 (en) 1993-12-17
JPH04506450A (en) 1992-11-12
WO1990010713A1 (en) 1990-09-20

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