DK0429587T3 - Process for Preparation of a Regulatory Protein of HIV-1 Using the Human Cell Line TH4-7-5 - Google Patents
Process for Preparation of a Regulatory Protein of HIV-1 Using the Human Cell Line TH4-7-5Info
- Publication number
- DK0429587T3 DK0429587T3 DK90908201.8T DK90908201T DK0429587T3 DK 0429587 T3 DK0429587 T3 DK 0429587T3 DK 90908201 T DK90908201 T DK 90908201T DK 0429587 T3 DK0429587 T3 DK 0429587T3
- Authority
- DK
- Denmark
- Prior art keywords
- cell line
- hiv
- preparation
- human cell
- regulatory protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
- C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
In a process for obtaining a regulation protein (nef) for HIV-1 using human astrocytes and/or glia cell lines, the cell line is infected with HIV-1 by coculturing, the infected cells are cloned and, if necessary, clones producing large quantities of nef are selected by sub-cloning. A particularly well suited cell line is the human cell line TH4-7-5 deposited with the Collection Nationale de Cultures de Microorganismes (CNCM) Paris, under the number I-861.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3917775 | 1989-05-31 | ||
DE19893940527 DE3940527C2 (en) | 1989-12-07 | 1989-12-07 | Human cell line TH4-7-5, its use and method for producing a protein product of an HIV regulatory gene |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0429587T3 true DK0429587T3 (en) | 1995-09-04 |
Family
ID=25881451
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK90908201.8T DK0429587T3 (en) | 1989-05-31 | 1990-05-31 | Process for Preparation of a Regulatory Protein of HIV-1 Using the Human Cell Line TH4-7-5 |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0429587B1 (en) |
AT (1) | ATE121135T1 (en) |
DE (1) | DE59008891D1 (en) |
DK (1) | DK0429587T3 (en) |
WO (1) | WO1990015150A1 (en) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1987002988A1 (en) * | 1985-11-07 | 1987-05-21 | President And Fellows Of Harvard College | T-cell lymphotrophic virus protein and assay |
-
1990
- 1990-05-31 EP EP90908201A patent/EP0429587B1/en not_active Expired - Lifetime
- 1990-05-31 WO PCT/EP1990/000869 patent/WO1990015150A1/en active IP Right Grant
- 1990-05-31 DK DK90908201.8T patent/DK0429587T3/en active
- 1990-05-31 DE DE59008891T patent/DE59008891D1/en not_active Expired - Fee Related
- 1990-05-31 AT AT90908201T patent/ATE121135T1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE59008891D1 (en) | 1995-05-18 |
EP0429587A1 (en) | 1991-06-05 |
EP0429587B1 (en) | 1995-04-12 |
WO1990015150A1 (en) | 1990-12-13 |
ATE121135T1 (en) | 1995-04-15 |
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