DK0429587T3 - Process for Preparation of a Regulatory Protein of HIV-1 Using the Human Cell Line TH4-7-5 - Google Patents

Process for Preparation of a Regulatory Protein of HIV-1 Using the Human Cell Line TH4-7-5

Info

Publication number
DK0429587T3
DK0429587T3 DK90908201.8T DK90908201T DK0429587T3 DK 0429587 T3 DK0429587 T3 DK 0429587T3 DK 90908201 T DK90908201 T DK 90908201T DK 0429587 T3 DK0429587 T3 DK 0429587T3
Authority
DK
Denmark
Prior art keywords
cell line
hiv
preparation
human cell
regulatory protein
Prior art date
Application number
DK90908201.8T
Other languages
Danish (da)
Inventor
Volker Erfle
Werner Mellert
Original Assignee
Gsf Forschungszentrum F
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19893940527 external-priority patent/DE3940527C2/en
Application filed by Gsf Forschungszentrum F filed Critical Gsf Forschungszentrum F
Application granted granted Critical
Publication of DK0429587T3 publication Critical patent/DK0429587T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

In a process for obtaining a regulation protein (nef) for HIV-1 using human astrocytes and/or glia cell lines, the cell line is infected with HIV-1 by coculturing, the infected cells are cloned and, if necessary, clones producing large quantities of nef are selected by sub-cloning. A particularly well suited cell line is the human cell line TH4-7-5 deposited with the Collection Nationale de Cultures de Microorganismes (CNCM) Paris, under the number I-861.
DK90908201.8T 1989-05-31 1990-05-31 Process for Preparation of a Regulatory Protein of HIV-1 Using the Human Cell Line TH4-7-5 DK0429587T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3917775 1989-05-31
DE19893940527 DE3940527C2 (en) 1989-12-07 1989-12-07 Human cell line TH4-7-5, its use and method for producing a protein product of an HIV regulatory gene

Publications (1)

Publication Number Publication Date
DK0429587T3 true DK0429587T3 (en) 1995-09-04

Family

ID=25881451

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90908201.8T DK0429587T3 (en) 1989-05-31 1990-05-31 Process for Preparation of a Regulatory Protein of HIV-1 Using the Human Cell Line TH4-7-5

Country Status (5)

Country Link
EP (1) EP0429587B1 (en)
AT (1) ATE121135T1 (en)
DE (1) DE59008891D1 (en)
DK (1) DK0429587T3 (en)
WO (1) WO1990015150A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0245459B1 (en) * 1985-11-07 1993-09-29 The President And Fellows Of Harvard College T-cell lymphotrophic virus protein and assay

Also Published As

Publication number Publication date
ATE121135T1 (en) 1995-04-15
DE59008891D1 (en) 1995-05-18
WO1990015150A1 (en) 1990-12-13
EP0429587A1 (en) 1991-06-05
EP0429587B1 (en) 1995-04-12

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