DK0418796T3 - Method for culturing cells in hollow microspheres - Google Patents

Method for culturing cells in hollow microspheres

Info

Publication number
DK0418796T3
DK0418796T3 DK90117893.9T DK90117893T DK0418796T3 DK 0418796 T3 DK0418796 T3 DK 0418796T3 DK 90117893 T DK90117893 T DK 90117893T DK 0418796 T3 DK0418796 T3 DK 0418796T3
Authority
DK
Denmark
Prior art keywords
culturing cells
hollow microspheres
hollow microbeads
cells
polyelectrolyte
Prior art date
Application number
DK90117893.9T
Other languages
Danish (da)
Inventor
Roland Dr Kurrle
Rainer Dr Buchholz
Carlo Dr Giani
Ulrich Fricke
Axel Dr Walch
Thomas Dr Bayer
Dieter Krause
Wiegand Lang
Asmus Dr Reiche
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Application granted granted Critical
Publication of DK0418796T3 publication Critical patent/DK0418796T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a process for the culture of cells which are entrapped in hollow microbeads and are densely packed in a fixed bed and are supplied with nutrients discontinuously or continuously in a vessel by the medium flow. The suitable hollow microbeads have a membrane composed of a polysaccharide gel or of a polyelectrolyte.
DK90117893.9T 1989-09-21 1990-09-18 Method for culturing cells in hollow microspheres DK0418796T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3931433A DE3931433A1 (en) 1989-09-21 1989-09-21 METHOD FOR CULTIVATING CELLS IN MICROHOLE BALLS

Publications (1)

Publication Number Publication Date
DK0418796T3 true DK0418796T3 (en) 1994-04-11

Family

ID=6389834

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90117893.9T DK0418796T3 (en) 1989-09-21 1990-09-18 Method for culturing cells in hollow microspheres

Country Status (9)

Country Link
EP (1) EP0418796B1 (en)
JP (1) JP2854951B2 (en)
KR (1) KR910006475A (en)
AT (1) ATE100856T1 (en)
CA (1) CA2025798A1 (en)
DE (2) DE3931433A1 (en)
DK (1) DK0418796T3 (en)
IE (1) IE64854B1 (en)
PT (1) PT95376B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7851189B2 (en) 2005-03-07 2010-12-14 Boston Scientific Scimed, Inc. Microencapsulated compositions for endoluminal tissue engineering

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0233899A1 (en) * 1985-06-24 1987-09-02 Karyon Technology, Incorporated Entrapment of anchorage-dependent cells
DE3735397A1 (en) * 1987-10-20 1989-05-03 Hoechst Ag MAGNETIC MEMBRANE CAPSULES AND THEIR USE

Also Published As

Publication number Publication date
PT95376A (en) 1991-05-22
DE59004397D1 (en) 1994-03-10
JP2854951B2 (en) 1999-02-10
DE3931433A1 (en) 1991-04-04
EP0418796B1 (en) 1994-01-26
CA2025798A1 (en) 1991-03-22
EP0418796A1 (en) 1991-03-27
DE3931433C2 (en) 1991-12-12
IE64854B1 (en) 1995-09-06
IE903404A1 (en) 1991-04-10
ATE100856T1 (en) 1994-02-15
PT95376B (en) 1997-07-31
JPH03119990A (en) 1991-05-22
KR910006475A (en) 1991-04-29

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