DK0409078T3 - Process for Sequencing of Deoxyribonucleic Acids - Google Patents

Process for Sequencing of Deoxyribonucleic Acids

Info

Publication number
DK0409078T3
DK0409078T3 DK90113328.0T DK90113328T DK0409078T3 DK 0409078 T3 DK0409078 T3 DK 0409078T3 DK 90113328 T DK90113328 T DK 90113328T DK 0409078 T3 DK0409078 T3 DK 0409078T3
Authority
DK
Denmark
Prior art keywords
concentration
acid
mol per
sequencing
deoxyribonucleic acids
Prior art date
Application number
DK90113328.0T
Other languages
Danish (da)
Inventor
Manfred Dr Wozny
Original Assignee
Basf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=6385388&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK0409078(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Basf Ag filed Critical Basf Ag
Application granted granted Critical
Publication of DK0409078T3 publication Critical patent/DK0409078T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The method comprises subjecting the deoxyribonucleic acid in a concentration of 10<-16> to 2 x 10<-14> mol per mu l, in the case of double-stranded deoxynucleic acid which is not in the supercoiled form preferably in a concentration of 10<-16> to 3 x 10<-15> mol per mu l, a) to a heat-denaturation step b) to an annealing step and c) to a nucleic acid synthesis step with partial chain termination, repeating steps a) to c) 10 to 60 times and subsequently fractionating the resulting deoxynucleic acid fragment mixture according to length, and determining the sequence from the fragment spectrum. o
DK90113328.0T 1989-07-19 1990-07-12 Process for Sequencing of Deoxyribonucleic Acids DK0409078T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3923895A DE3923895A1 (en) 1989-07-19 1989-07-19 METHOD FOR SEQUENCING DESOXYRIBONUCLEIC ACIDS

Publications (1)

Publication Number Publication Date
DK0409078T3 true DK0409078T3 (en) 1994-12-05

Family

ID=6385388

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90113328.0T DK0409078T3 (en) 1989-07-19 1990-07-12 Process for Sequencing of Deoxyribonucleic Acids

Country Status (6)

Country Link
EP (1) EP0409078B1 (en)
JP (1) JPH0813279B2 (en)
AT (1) ATE113994T1 (en)
DE (2) DE3923895A1 (en)
DK (1) DK0409078T3 (en)
ES (1) ES2063206T3 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993002212A1 (en) * 1991-07-24 1993-02-04 University Partnerships Pty. Ltd. Single step amplification and sequencing of nucleic acids
DE4214112A1 (en) * 1991-08-02 1993-02-04 Europ Lab Molekularbiolog NEW METHOD FOR SEQUENCING NUCLEIC ACIDS
US5674679A (en) * 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
DE19612684A1 (en) * 1996-03-29 1997-10-02 Gsf Forschungszentrum Umwelt New use of extremely thermophilic DNA polymerases
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3930312A1 (en) * 1988-10-24 1990-04-26 Max Planck Gesellschaft METHOD FOR DIRECTLY SEQUENCING NUCLEIC ACID CHAINS

Also Published As

Publication number Publication date
EP0409078A2 (en) 1991-01-23
JPH0372899A (en) 1991-03-28
DE3923895A1 (en) 1991-01-24
ATE113994T1 (en) 1994-11-15
EP0409078B1 (en) 1994-11-09
ES2063206T3 (en) 1995-01-01
JPH0813279B2 (en) 1996-02-14
DE59007676D1 (en) 1994-12-15
EP0409078A3 (en) 1991-07-17

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