DK0393684T3 - Recombinant DNA and expression vector - Google Patents

Recombinant DNA and expression vector

Info

Publication number
DK0393684T3
DK0393684T3 DK90107465.8T DK90107465T DK0393684T3 DK 0393684 T3 DK0393684 T3 DK 0393684T3 DK 90107465 T DK90107465 T DK 90107465T DK 0393684 T3 DK0393684 T3 DK 0393684T3
Authority
DK
Denmark
Prior art keywords
recombinant dna
gene
expression vector
sequence
brought
Prior art date
Application number
DK90107465.8T
Other languages
Danish (da)
Inventor
August Prof Dr Boeck
Robert Gary Dr Rer Nat Sawers
Michael Dr Rer Nat Jarsch
Roland Herbst
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Application granted granted Critical
Publication of DK0393684T3 publication Critical patent/DK0393684T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The recombinant DNA contains a regulatory region which is at least 50 % homologous with the subregions -969 to -991 base pairs and/or -1308 to -1330 base pairs of the sequence of Figure 10, and contains in the 3' direction from the regulatory sequence at least one promoter region which in turn contains a -35/-10 promoter sequence. An expression vector according to the invention contains a recombinant DNA according to the invention ligated into a suitable vector molecule. To prepare the recombinant DNA according to the invention, the pfl gene with its upstream regions is isolated from the gene bank of a microorganism which contains the gene, and the required parts are obtained by methods known per se and joined to the other required sequences or promoters. When a recombinant DNA according to the invention or an expression vector according to the invention is used for the inducible and repressible expression of a foreign gene, the induction is brought about under anaerobic conditions and by pyruvate, and the repression is brought about by oxygen.
DK90107465.8T 1989-04-21 1990-04-19 Recombinant DNA and expression vector DK0393684T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3913201 1989-04-21
DE3926076A DE3926076A1 (en) 1989-04-21 1989-08-07 RECOMBINANT DNA AND EXPRESSION VECTOR

Publications (1)

Publication Number Publication Date
DK0393684T3 true DK0393684T3 (en) 1998-01-26

Family

ID=25880155

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90107465.8T DK0393684T3 (en) 1989-04-21 1990-04-19 Recombinant DNA and expression vector

Country Status (7)

Country Link
EP (1) EP0393684B1 (en)
JP (1) JP2774989B2 (en)
AT (1) ATE154639T1 (en)
CA (1) CA2015046C (en)
DE (2) DE3926076A1 (en)
DK (1) DK0393684T3 (en)
ES (1) ES2104568T3 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE180280T1 (en) * 1991-03-05 1999-06-15 Wellcome Found EXPRESSION OF RECOMBINANT PROTEINS IN ATTENUATE BACTERIA
GB9104596D0 (en) * 1991-03-05 1991-04-17 Wellcome Found Production of recombinant proteins
DE4136389A1 (en) * 1991-03-18 1992-10-08 Hoechst Ag PROCESS FOR THE PRODUCTION OF GLUTARYLACYLASE IN LARGE QUANTITIES
US5830692A (en) * 1995-03-24 1998-11-03 Consortium Fur Elektrochemische Industrie Gmbh Expression system which can be regulated
KR101643195B1 (en) * 2014-10-06 2016-07-27 경일대학교산학협력단 Helmet apparatus for detecting object under water and method thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3735381A1 (en) * 1987-03-31 1989-05-03 Boehringer Mannheim Gmbh RECOMBINANT DNA FOR REPRESSIBLE AND INDUCIBLE EXPRESSION OF FOREIGN GENES

Also Published As

Publication number Publication date
JPH0380088A (en) 1991-04-04
EP0393684A1 (en) 1990-10-24
ES2104568T3 (en) 1997-10-16
JP2774989B2 (en) 1998-07-09
EP0393684B1 (en) 1997-06-18
DE3926076A1 (en) 1990-10-25
CA2015046C (en) 2003-09-16
ATE154639T1 (en) 1997-07-15
DE59010726D1 (en) 1997-07-24
CA2015046A1 (en) 1990-10-21

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