DE923738C - Process for the enrichment of tuberculocidal active substances - Google Patents

Process for the enrichment of tuberculocidal active substances

Info

Publication number
DE923738C
DE923738C DEI3471A DEI0003471A DE923738C DE 923738 C DE923738 C DE 923738C DE I3471 A DEI3471 A DE I3471A DE I0003471 A DEI0003471 A DE I0003471A DE 923738 C DE923738 C DE 923738C
Authority
DE
Germany
Prior art keywords
enrichment
active substances
tuberculocidal
extracts
palmitic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEI3471A
Other languages
German (de)
Inventor
Erich Dr Med Hesse
Karl-Heinz Dr Rer Nat Imhausen
Gustav Dr Rer Nat Renckhoff
Walter Dr Rer Nat Rudolph
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imhausen & Co GmbH
Original Assignee
Imhausen & Co GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imhausen & Co GmbH filed Critical Imhausen & Co GmbH
Priority to DEI3471A priority Critical patent/DE923738C/en
Application granted granted Critical
Publication of DE923738C publication Critical patent/DE923738C/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
    • C07G11/00—Antibiotics

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Description

Verfahren zur Anreicherung tuberculocider Wirkstoffe In Escherichia coli bzw. in den Kulturflüssigkeiten von Escherichia coli sind antibiotisch wirksame Stoffe enthalten, die man als angereicherte Extrakte gewinnen kann, indem man beispielsweise die Bakterienmasse oder die Kulturflüssigkeiten von hesonders ausgelesenen aktiven Colistämmen mit lipoidlösenden Lösungsmitteln extrahiert. Als Ausgangsmaterial für die Gewinnung solcher Extrakte kann man auch Colibakterien benutzen, die nicht in besonders künstlich angelegten Kulturen gewonnen werden, sondern es können dazu auch Fäkalien solcher Lebewesen benutzt werden, die Escherichia coli in ihrem Verdauungstraktus beherbergen.Process for the enrichment of tuberculocidal active substances In Escherichia coli or in the culture fluids of Escherichia coli are antibiotically effective Contain substances that can be obtained as enriched extracts, for example by the bacterial mass or the culture fluids of specially selected active Coli strains extracted with lipoid-dissolving solvents. As a raw material for Coli bacteria can also be used to obtain such extracts, which are not found in especially artificially created cultures can be obtained, but it can do so feces of organisms such as Escherichia coli are also used in their digestive tract accommodate.

Solche Fäkalien sind jedoch im allgemeinen weniger geeignet als besonders gezüchtete antibiotisch aktive Stämme.However, such feces are generally less suitable than particularly cultivated antibiotic active strains.

Es wurde nun gefunden, daß die durch Extraktion mit lipoidlösenden Lösungsmitteln erhaltenen Extrakte, unabhängig von ihrer Herkunft, höhermolekulare gesättigte Fettsäuren, vorzugsweise Palmitinsäure, enthalten, und daß durch deren Abtrennung die tuberculocide Wirkung der Extrakte ganz außerordentlich ansteigt. It has now been found that by extraction with lipoid-dissolving Extracts obtained from solvents, regardless of their origin, are of higher molecular weight saturated fatty acids, preferably palmitic acid, and that by their Separation the tuberculocide effect of the extracts increases quite extraordinarily.

Beispiele I. 20 g eines Extraktes von Bact. coli, der durch Ätherextraktion eines im Vakuum aus der Bakterienmasse hergestellten Trockenpulvers erhalten worden war, zeigt bei der Prüfung seiner Bacterici die gegenüber Tuberkelbazillen noch eine sichere Wirkung in der Verdünnung I: 10000. Durch Ausfrieren der Palmitinsäure aus diesem Extrakt erhält man nach Absaugen der kristallisierten Anteile 3 bis 4 g eines Öls, das sich bis zur Verdünnung I.: 50000 bactericid wirkt. Examples I. 20 g of an extract from Bact. coli produced by ether extraction a dry powder produced in a vacuum from the bacterial mass been was, in the examination of its Bacterici, the opposite to tubercle bacilli still shows a safe effect in the dilution I: 10000. By freezing out the palmitic acid 3 to 4 are obtained from this extract after the crystallized portions have been filtered off with suction g of an oil that has a bactericidal effect up to the dilution I .: 50000.

2. Durch Ätherextraktion von im Vakuum bei einer Temperatur von maximal 400 getrocknetem menschlichem Fäces erhält man nach restloser Entfernung des Extraktionsmittels ein Ö1, das teilweise zum Kristallisieren neigt. Die tuberculociden Eigenschaften dieses Öls werden durch Entfernung dieser Kristalle, die aus Palmitinsäure bestehen, auf das Doppelte gesteigert. 2. By ether extraction in a vacuum at a temperature of maximum 400 dried human feces are obtained after the extraction agent has been completely removed an oil that partially tends to crystallize. The tuberculocidal properties of this oil, by removing these crystals, which consist of palmitic acid, doubled.

Claims (1)

PATENTANSPRUCH. PATENT CLAIM. Verfahren zur Anreicherung tuberculocider Wirkstoffe in antibiotisch wirkenden Extrakten aus Escherichia coli mit lipoidlösenden Lösungsmitteln, dadurch gekennzeichnet, daß man aus diesen Extrakten höhermolekulare gesättigte Fettsäuren, insbesondere Palmitinsäure, entfernt. Process for the enrichment of tuberculocider active substances in antibiotic effective extracts from Escherichia coli with lipoid-dissolving solvents, thereby characterized in that from these extracts higher molecular weight saturated fatty acids, especially palmitic acid. Angezogene Druckschriften: Ann. Inst. Pasteur, I947, 5. 904; Hoppe-Seyler, I950, S. I72; Lembke und Krüger-Thiemer, Jena, I952, Literaturstudien zum Tuberkuloseproblem. Printed publications: Ann. Inst. Pasteur, 1947, pp. 904; Hoppe-Seyler, 1950, p. 172; Lembke and Krüger-Thiemer, Jena, I952, literature studies on the tuberculosis problem.
DEI3471A 1950-11-04 1950-11-04 Process for the enrichment of tuberculocidal active substances Expired DE923738C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEI3471A DE923738C (en) 1950-11-04 1950-11-04 Process for the enrichment of tuberculocidal active substances

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEI3471A DE923738C (en) 1950-11-04 1950-11-04 Process for the enrichment of tuberculocidal active substances

Publications (1)

Publication Number Publication Date
DE923738C true DE923738C (en) 1955-02-21

Family

ID=7184669

Family Applications (1)

Application Number Title Priority Date Filing Date
DEI3471A Expired DE923738C (en) 1950-11-04 1950-11-04 Process for the enrichment of tuberculocidal active substances

Country Status (1)

Country Link
DE (1) DE923738C (en)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

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