DE903502C - Process for the production of a highly effective growth substance - Google Patents

Description

Process for the production of a highly effective growth substance There are several proposals are already known to promote organic growth. the The best results have so far been achieved with products that are called vitamin T complexes are designated.

According to the invention, a method is now shown, which a .Das growth-promoting substance supplies the well-known effects in its effect far exceeds.

The process consists in letting casein undergo hydrolysis of papain and! preferably cysteine or hydrogen sulfide in dilute alcohol is subjected at a slightly elevated temperature. All alcohol is preferred Ethyl alcohol. optionally a higher alcohol is used.

The product obtained in this way acts, for example, on Tribolium confusum and tissue cultures. strong growth; promotional. So it is only with him z. B. possible to achieve an image formation (beetle formation). Even tissue cultures that are difficult grew, are strongly stimulated to growth by this product.

Zoo g casein, vitamin-free, Merck earthed for 10 hours with the addition of 2 g Papai.n Merck, i: 350 and 0.3 g cysteine hydrochloride and 500 cc 60% ethyl alcohol hydrolyzed at 40 to 45 ° in a water bath. After one hour of hydrolysis, the flask remains in the water bath overnight. The next day the hydrolysis is carried out again at 40 to 45 ° 10 hours. The flask remains in the water bath again overnight. The next day the entire contents of the koilben are filtered. The normal filtrate is concentrated in a circulation evaporator at about 30 ° until the first precipitates appear. Then it is carefully concentrated on the water bath and dried. The ground dry matter must show a strongly positive biuretre @ action.

The remaining casein residue! is inactive. The protein body obtained from the filtrate can be tested again for the first time. This rice flour beetle Trnbolium confusum is used as a test. The test diet for the highly effective active ingredient has the following composition: 95 0/0 rice starch (vitamin-free), 5 0 / a salts (McCollum salt mixture).

Follow this diet per gram of the carbohydrate-salt mixture Vitamins eye set: 7 Gamma Thiamin ', 7 Gamma La.kto flavin, 35 Gamma Pyri, doxin, 35 gamma nicotinic acid amide, 7o gammia, pantothenic acid Ca, io gamma folic acid, o, 4 Gamma biogin, s mg choline chloride, i mg ergosberine.

The casein preparation to be tested corresponds to this one described above Diet added.

The flour beetle Tribollum confu.sum shows the following development times with the presence of eight lovingly necessary vitamins, carbohydrates, at least 60% casein (normal casein), 0.1% ergosterol and 50% sulphate: Development in the egg ... ............ 5 days hatching of the almost 1 mm larva from the egg on .................... 5th and 6th Days of growth of the almost 1 mm large larva up to 7 to 7.5 mm large larva ................. 24 days pupation of larvae on the 24th to a5. Days of pupal rest ..................... 5 days of hatching beetle after 5 days of pupal rest. This excellent test object, which happens to need the same active ingredients as humans and animals, does not develop if only one of the vitamins is missing or the protein diet (casein diet) is reduced.

Many attempts to replace the protein component with essential amino acids or Replacing @di by all possible combinations leaves the larvae only up to the pupa grow. The pupa never develops into a beetle. The one above for the first time described protein body made possible with 100% certainty by adding only i% to the essential amino acids the development of the pupa to the beetle. For this We use this new protein factor, which is strongly biuret-positive, as an image factor designated. If only 2.5 to 5% is added to the above-mentioned diet, the entire Casein content (.55 to 6o%) can be omitted.

The body was also shown to be in hard-to-grow tissue cultures highly active.

Even with mouse experiments it turned out that the new one Found protein bodies essential protein in the widest possible range. Saving dimensions and- able to replace.

Claims (1)

PATENT CLAIMS: i. Method of producing a highly effective Wuchssboffes, marked @, i that casein with one more than i carbon atom containing. Alcohol, and papain, or preferably also in the presence of cysteine or hydrogen sulfide 'is hydrolyzed. Procedure. according to claim i, characterized characterized, d'ass ethyl alcohol applied at temperatures of about 3o to 40 ° will.