DE69937453D1 - PROCESS FOR THE ISOLATION AND CHARACTERIZATION OF POTENTIAL FUNCTIONS BASED ON A BIOLOGICAL SAMPLE CONTAINING NUCLEIC ACIDS - Google Patents
PROCESS FOR THE ISOLATION AND CHARACTERIZATION OF POTENTIAL FUNCTIONS BASED ON A BIOLOGICAL SAMPLE CONTAINING NUCLEIC ACIDSInfo
- Publication number
- DE69937453D1 DE69937453D1 DE69937453T DE69937453T DE69937453D1 DE 69937453 D1 DE69937453 D1 DE 69937453D1 DE 69937453 T DE69937453 T DE 69937453T DE 69937453 T DE69937453 T DE 69937453T DE 69937453 D1 DE69937453 D1 DE 69937453D1
- Authority
- DE
- Germany
- Prior art keywords
- characterization
- isolation
- nucleic acids
- biological sample
- vector molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 239000012472 biological sample Substances 0.000 title abstract 2
- 238000012512 characterization method Methods 0.000 title abstract 2
- 238000002955 isolation Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 239000012634 fragment Substances 0.000 abstract 5
- 238000010276 construction Methods 0.000 abstract 2
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Abstract
The present invention is directed to a process for the separation and characterization of the functions potentially present in a biological sample containing nucleic acids, characterized in that it comprises the steps of preparation of nucleic acid fragments starting from said sample, association of each one of said fragments with a vector molecule, isolation of each fragment associated with a vector molecule or with a part of each construction composed of a fragment associated with a vector molecule, in vitro treatment of each fragment associated with a vector molecule or of a part of each construction composed of a fragment associated with a vector molecule to obtain transcripts, and testing of the function of the transcripts or of the proteins encoded thereby after translation of said transcripts.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9810337 | 1998-08-12 | ||
| FR9810337A FR2782325B1 (en) | 1998-08-12 | 1998-08-12 | METHOD OF IDENTIFYING POLYNUCLEOTIDE SEQUENCES AND / OR CORRESPONDING PROTEINS FROM A SAMPLE OF NUCLEIC ACIDS |
| PCT/FR1999/001972 WO2000009747A1 (en) | 1998-08-12 | 1999-08-11 | Method for separating and characterising functions potentially present in a biological sample containing nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69937453D1 true DE69937453D1 (en) | 2007-12-13 |
| DE69937453T2 DE69937453T2 (en) | 2008-08-21 |
Family
ID=9529620
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69937453T Expired - Lifetime DE69937453T2 (en) | 1998-08-12 | 1999-08-11 | PROCESS FOR THE ISOLATION AND CHARACTERIZATION OF POTENTIAL FUNCTIONS BASED ON A BIOLOGICAL SAMPLE CONTAINING NUCLEIC ACIDS |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US6514703B1 (en) |
| EP (1) | EP1104489B1 (en) |
| JP (1) | JP2002522093A (en) |
| KR (1) | KR100694929B1 (en) |
| CN (1) | CN100422340C (en) |
| AT (1) | ATE377092T1 (en) |
| AU (1) | AU766252B2 (en) |
| BR (1) | BR9912934A (en) |
| CA (1) | CA2339904A1 (en) |
| DE (1) | DE69937453T2 (en) |
| DK (1) | DK1104489T3 (en) |
| ES (1) | ES2296397T3 (en) |
| FR (1) | FR2782325B1 (en) |
| IL (2) | IL141372A0 (en) |
| NZ (1) | NZ509766A (en) |
| RU (1) | RU2249045C2 (en) |
| WO (1) | WO2000009747A1 (en) |
| ZA (1) | ZA200101288B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0012233D0 (en) * | 2000-05-19 | 2000-07-12 | Devgen Nv | Vector constructs |
| AU2006272706A1 (en) * | 2005-07-21 | 2007-02-01 | Abbvie Inc. | Multiple gene expression including sorf constructs and methods with polyproteins, pro-proteins, and proteolysis |
| EP2493923A1 (en) * | 2009-10-30 | 2012-09-05 | Abbott Laboratories | Sorf constructs and multiple gene expression |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU4434585A (en) * | 1985-03-30 | 1986-10-23 | Marc Ballivet | Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique |
| US5571690A (en) * | 1991-05-08 | 1996-11-05 | The University Of Virginia Patents Foundation | Method for the cell-free synthesis of proteins |
| KR960701892A (en) * | 1993-04-08 | 1996-03-28 | 제임스 에프. 와일러 | Cell free system for protein synthesis and use of chaperone proteins therein |
| US6037329A (en) * | 1994-03-15 | 2000-03-14 | Selective Genetics, Inc. | Compositions containing nucleic acids and ligands for therapeutic treatment |
| US5643722A (en) * | 1994-05-11 | 1997-07-01 | Trustees Of Boston University | Methods for the detection and isolation of proteins |
| TW299352B (en) * | 1994-11-03 | 1997-03-01 | Res Dev Foundation | |
| BR9607267A (en) * | 1995-02-27 | 1998-12-15 | Intelligene Ltd | Detection of biomolecules |
| JPH11503611A (en) * | 1995-04-11 | 1999-03-30 | トラスティーズ・オブ・ボストン・ユニバーシティ | Solid-phase sequencing of biopolymers |
| CN1167151A (en) * | 1997-04-02 | 1997-12-10 | 中国科学院上海生物化学研究所 | Method for deciding relative translation initial rate of external source genes in colibacillus |
| WO1998049274A1 (en) * | 1997-04-29 | 1998-11-05 | Appligene-Oncor | Thermostable dna polymerase and inteins of the thermococcus fumicolans species |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| CA2326827A1 (en) * | 1998-05-05 | 1999-11-11 | Gene Logic, Inc. | A process to study changes in gene expression in t lymphocytes |
| US6303337B1 (en) * | 1999-08-25 | 2001-10-16 | Amber Gen. Inc. | N-terminal and C-terminal markers in nascent proteins |
-
1998
- 1998-08-12 FR FR9810337A patent/FR2782325B1/en not_active Expired - Fee Related
-
1999
- 1999-08-11 CA CA002339904A patent/CA2339904A1/en not_active Abandoned
- 1999-08-11 AU AU51716/99A patent/AU766252B2/en not_active Ceased
- 1999-08-11 CN CNB998120367A patent/CN100422340C/en not_active Expired - Fee Related
- 1999-08-11 EP EP99936724A patent/EP1104489B1/en not_active Expired - Lifetime
- 1999-08-11 DE DE69937453T patent/DE69937453T2/en not_active Expired - Lifetime
- 1999-08-11 WO PCT/FR1999/001972 patent/WO2000009747A1/en active IP Right Grant
- 1999-08-11 DK DK99936724T patent/DK1104489T3/en active
- 1999-08-11 NZ NZ509766A patent/NZ509766A/en not_active IP Right Cessation
- 1999-08-11 JP JP2000565181A patent/JP2002522093A/en active Pending
- 1999-08-11 IL IL14137299A patent/IL141372A0/en active IP Right Grant
- 1999-08-11 AT AT99936724T patent/ATE377092T1/en not_active IP Right Cessation
- 1999-08-11 ES ES99936724T patent/ES2296397T3/en not_active Expired - Lifetime
- 1999-08-11 BR BR9912934-5A patent/BR9912934A/en not_active IP Right Cessation
- 1999-08-11 RU RU2001106643/13A patent/RU2249045C2/en not_active IP Right Cessation
- 1999-08-11 KR KR1020017001845A patent/KR100694929B1/en not_active IP Right Cessation
-
2000
- 2000-11-28 US US09/722,392 patent/US6514703B1/en not_active Expired - Lifetime
-
2001
- 2001-02-11 IL IL141372A patent/IL141372A/en not_active IP Right Cessation
- 2001-02-15 ZA ZA200101288A patent/ZA200101288B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US6514703B1 (en) | 2003-02-04 |
| ATE377092T1 (en) | 2007-11-15 |
| ZA200101288B (en) | 2002-02-15 |
| EP1104489B1 (en) | 2007-10-31 |
| EP1104489A1 (en) | 2001-06-06 |
| NZ509766A (en) | 2004-01-30 |
| RU2249045C2 (en) | 2005-03-27 |
| CN1323356A (en) | 2001-11-21 |
| AU5171699A (en) | 2000-03-06 |
| KR100694929B1 (en) | 2007-03-14 |
| ES2296397T3 (en) | 2008-04-16 |
| IL141372A0 (en) | 2002-03-10 |
| IL141372A (en) | 2008-03-20 |
| CN100422340C (en) | 2008-10-01 |
| FR2782325B1 (en) | 2002-05-24 |
| FR2782325A1 (en) | 2000-02-18 |
| JP2002522093A (en) | 2002-07-23 |
| DE69937453T2 (en) | 2008-08-21 |
| CA2339904A1 (en) | 2000-02-24 |
| RU2001106643A (en) | 2004-03-27 |
| DK1104489T3 (en) | 2008-02-25 |
| WO2000009747A1 (en) | 2000-02-24 |
| BR9912934A (en) | 2001-05-08 |
| AU766252B2 (en) | 2003-10-09 |
| KR20010072442A (en) | 2001-07-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |