DE69907266D1 - Synthese und gewinnung von aspartam, unter anwendung eines enzymatischen deformylationsschrittes - Google Patents

Synthese und gewinnung von aspartam, unter anwendung eines enzymatischen deformylationsschrittes

Info

Publication number
DE69907266D1
DE69907266D1 DE69907266T DE69907266T DE69907266D1 DE 69907266 D1 DE69907266 D1 DE 69907266D1 DE 69907266 T DE69907266 T DE 69907266T DE 69907266 T DE69907266 T DE 69907266T DE 69907266 D1 DE69907266 D1 DE 69907266D1
Authority
DE
Germany
Prior art keywords
enzymatic
synthesis
aspartame
formyl
deformylation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69907266T
Other languages
English (en)
Other versions
DE69907266T2 (de
Inventor
Jan Quaedflieg
Theodorus Sonke
Fritz Wagner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Holland Sweetener Co VOF
Original Assignee
Holland Sweetener Co VOF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Holland Sweetener Co VOF filed Critical Holland Sweetener Co VOF
Priority claimed from PCT/NL1999/000787 external-priority patent/WO2000037486A1/en
Publication of DE69907266D1 publication Critical patent/DE69907266D1/de
Application granted granted Critical
Publication of DE69907266T2 publication Critical patent/DE69907266T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06104—Dipeptides with the first amino acid being acidic
    • C07K5/06113—Asp- or Asn-amino acid
    • C07K5/06121—Asp- or Asn-amino acid the second amino acid being aromatic or cycloaliphatic
    • C07K5/0613—Aspartame
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Seasonings (AREA)
DE69907266T 1998-12-22 1999-12-20 Synthese und gewinnung von aspartam, unter anwendung eines enzymatischen deformylationsschrittes Expired - Lifetime DE69907266T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP98204373A EP1013663A1 (de) 1998-12-22 1998-12-22 Synthese und Gewinnung von Aspartam unter Anwendung eines enzymatischen Deformylierungsschrittes
EP98204373 1998-12-22
PCT/NL1999/000787 WO2000037486A1 (en) 1998-12-22 1999-12-20 Synthesis and recovery of aspartame involving enzymatic deformylation step

Publications (2)

Publication Number Publication Date
DE69907266D1 true DE69907266D1 (de) 2003-05-28
DE69907266T2 DE69907266T2 (de) 2004-03-25

Family

ID=8234517

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69907266T Expired - Lifetime DE69907266T2 (de) 1998-12-22 1999-12-20 Synthese und gewinnung von aspartam, unter anwendung eines enzymatischen deformylationsschrittes

Country Status (6)

Country Link
EP (2) EP1013663A1 (de)
KR (1) KR20010099875A (de)
CN (1) CN1178953C (de)
AT (1) ATE238344T1 (de)
AU (1) AU1898500A (de)
DE (1) DE69907266T2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102864196B (zh) * 2012-10-09 2014-07-02 南京工业大学 一种二氢乳清酸酶法制备α-天冬氨酰小肽的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS585189A (ja) * 1981-07-01 1983-01-12 Sanraku Inc 新規なアミドヒドロラ−ゼ
JPS60164495A (ja) * 1984-01-16 1985-08-27 モンサント　コンパニー Ｎ‐ホルミルアミノ酸とペプチド残基の酵素的結合法
IT1176332B (it) * 1984-06-27 1987-08-18 Erba Farmitalia Procedimento per al preparazione di peptidi
US4745067A (en) * 1985-05-02 1988-05-17 Microbial Chemistry Research Foundation L-aminoacylases
DE69726868T2 (de) * 1996-07-19 2004-10-28 Monsanto Technology Llc. DEFORMYLIERUNG VON f-MET PEPTIDEN IN BAKTERIEN EXPRESSIONSSYSTEMEN

Also Published As

Publication number Publication date
AU1898500A (en) 2000-07-12
CN1178953C (zh) 2004-12-08
KR20010099875A (ko) 2001-11-09
ATE238344T1 (de) 2003-05-15
EP1013663A1 (de) 2000-06-28
CN1335851A (zh) 2002-02-13
EP1140982A1 (de) 2001-10-10
EP1140982B1 (de) 2003-04-23
DE69907266T2 (de) 2004-03-25

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