DE69618776T2 - BIOCHEMICAL DETECTION METHOD COUPLED WITH A CONTINUOUS SEPARATION TECHNOLOGY - Google Patents

BIOCHEMICAL DETECTION METHOD COUPLED WITH A CONTINUOUS SEPARATION TECHNOLOGY

Info

Publication number
DE69618776T2
DE69618776T2 DE69618776T DE69618776T DE69618776T2 DE 69618776 T2 DE69618776 T2 DE 69618776T2 DE 69618776 T DE69618776 T DE 69618776T DE 69618776 T DE69618776 T DE 69618776T DE 69618776 T2 DE69618776 T2 DE 69618776T2
Authority
DE
Germany
Prior art keywords
affinity molecule
effluent
controlled amount
detection
detectable ligand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69618776T
Other languages
German (de)
Other versions
DE69618776D1 (en
Inventor
Der Greef Jan Van
Hubertus Irth
Aaike Johannes Oosterkamp
Saskia Lutz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SCREENTEC BV
Original Assignee
SCREENTEC BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SCREENTEC BV filed Critical SCREENTEC BV
Application granted granted Critical
Publication of DE69618776D1 publication Critical patent/DE69618776D1/en
Publication of DE69618776T2 publication Critical patent/DE69618776T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8429—Preparation of the fraction to be distributed adding modificating material
    • G01N2030/8435—Preparation of the fraction to be distributed adding modificating material for chemical reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8804—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 automated systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8827—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/884—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample organic compounds

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

The present invention relates to an on-line detection method comprising the on-line coupling of a fractionation and a biochemical detection technique, which method comprises the addition of a controlled amount of an affinity molecule to the effluent of the fractionation step to react with analytes in the effluent, the subsequent addition of a controlled amount of a detectable ligand capable of binding to the affinity molecule, and detection of the affinity molecule/detectable ligand complex. Further, the invention is directed to an on-line method for the screening of compounds on their binding capability to a known affinity molecule, which method comprises a fractionation step providing an effluent, the addition of a controlled amount of said affinity molecule to the effluent of the fractionation step and effecting a contact time sufficient to allow a reaction with or interaction between the compounds in the effluent, the subsequent addition of a controlled amount of a detectable ligand capable of binding to the affinity molecule, and detection of the affinity molecule/detectable ligand complex.
DE69618776T 1995-04-21 1996-04-19 BIOCHEMICAL DETECTION METHOD COUPLED WITH A CONTINUOUS SEPARATION TECHNOLOGY Expired - Lifetime DE69618776T2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP95201029 1995-04-21
EP95202987 1995-11-03
PCT/NL1996/000173 WO1996033409A1 (en) 1995-04-21 1996-04-19 On-line coupling of biochemical detection to continuous-flow separation techniques

Publications (2)

Publication Number Publication Date
DE69618776D1 DE69618776D1 (en) 2002-03-14
DE69618776T2 true DE69618776T2 (en) 2002-10-17

Family

ID=26139240

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69618776T Expired - Lifetime DE69618776T2 (en) 1995-04-21 1996-04-19 BIOCHEMICAL DETECTION METHOD COUPLED WITH A CONTINUOUS SEPARATION TECHNOLOGY

Country Status (10)

Country Link
EP (1) EP0821790B1 (en)
JP (1) JP3739399B2 (en)
AT (1) ATE212441T1 (en)
AU (1) AU5409296A (en)
DE (1) DE69618776T2 (en)
DK (1) DK0821790T3 (en)
ES (1) ES2170229T3 (en)
PT (1) PT821790E (en)
SI (1) SI0821790T1 (en)
WO (1) WO1996033409A1 (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5183740A (en) * 1990-02-23 1993-02-02 The United States Of America As Represented By The Secretary Of The Navy Flow immunosensor method and apparatus

Also Published As

Publication number Publication date
DK0821790T3 (en) 2002-05-13
SI0821790T1 (en) 2002-04-30
DE69618776D1 (en) 2002-03-14
WO1996033409A1 (en) 1996-10-24
JP3739399B2 (en) 2006-01-25
JPH11509922A (en) 1999-08-31
ATE212441T1 (en) 2002-02-15
ES2170229T3 (en) 2002-08-01
EP0821790B1 (en) 2002-01-23
AU5409296A (en) 1996-11-07
EP0821790A1 (en) 1998-02-04
PT821790E (en) 2002-07-31

Similar Documents

Publication Publication Date Title
EP0657737A3 (en) Method and reagents for binding chemical analytes to a substrate surface, and related analytical devices and diagnostic techniques.
AU4827990A (en) Apparatus and method for sequential determination of an analyte in a fluid sample
AU622082B2 (en) Method for spectroscopic analysis of hydrocarbons, and hydrocarbon refining operations using same
GR3004894T3 (en) Method, system and devices for the assay and detection of biochemical molecules
GR3007381T3 (en)
DE59814295D1 (en) Method for determining analytes using a universal analysis element
CA2163895A1 (en) Simple immunochemical semi-quantitative assay method and apparatus
WO1995027210A1 (en) Analysis method and analysis apparatus
EP0637750A3 (en) Method for analysing sample fluids.
AU7044787A (en) Internal references for use in analyte-receptor assays
HU9402170D0 (en) Homocysteine assay
ATE256119T1 (en) METHOD FOR DETECTING A TARGET SUBSTANCE IN A SAMPLE USING PYRYLIUM COMPOUND
WO1999045374A3 (en) Purification and detection processes using reversible affinity electrophoresis
AU8917291A (en) Method for quantitatively assaying the presence fo diarrhetic shellfish poisoning toxins in marine samples.
AU4553296A (en) Method and reagent for the specific determination of LDL in serum samples
AU3996693A (en) Process for the immunochemical determination of an analyte
DE69618776D1 (en) BIOCHEMICAL DETECTION METHOD COUPLED WITH A CONTINUOUS SEPARATION TECHNOLOGY
GB9025471D0 (en) Method of assay
AU7198391A (en) Functional assay for determining the protein s activity
PT630968E (en) SELECTION BY GENETIC TECHNOLOGY OF PROTEINS CAPABLE OF LIGACATION TO LIGANDS BY SIGNAL TRANSDUCTION IN MICROORGANISMS
CA2193344A1 (en) Immunological determination method
AU2868489A (en) Assays for incipient diabetes, monoclonal antibodies used therein and kits for use therefor
AU5007890A (en) Device and process for the visualization of substance stains on layer chromatograms
WO1999064584A3 (en) Protein for regulating apoptosis
AU3692893A (en) Diagnostic method for the immunological determination of one or more analytes

Legal Events

Date Code Title Description
8364 No opposition during term of opposition