DE69232535D1 - LONG-TERM CULTURE HORMONE-SECONDING PANCREATIC CELLS - Google Patents

LONG-TERM CULTURE HORMONE-SECONDING PANCREATIC CELLS

Info

Publication number
DE69232535D1
DE69232535D1 DE69232535A DE69232535A DE69232535D1 DE 69232535 D1 DE69232535 D1 DE 69232535D1 DE 69232535 A DE69232535 A DE 69232535A DE 69232535 A DE69232535 A DE 69232535A DE 69232535 D1 DE69232535 D1 DE 69232535D1
Authority
DE
Germany
Prior art keywords
cells
hormone
secretion
insulin
respond
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69232535A
Other languages
German (de)
Other versions
DE69232535T2 (en
DE69232535T4 (en
Inventor
Janice Brothers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hcell Technology Inc Reno Nv Us
Original Assignee
Pacific Biomedical Research Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pacific Biomedical Research Inc filed Critical Pacific Biomedical Research Inc
Application granted granted Critical
Publication of DE69232535D1 publication Critical patent/DE69232535D1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0613—Cells from endocrine organs
    • C12N5/0617—Thyroid and parathyroid glands
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/31—Pituitary sex hormones, e.g. follicle-stimulating hormone [FSH], luteinising hormone [LH]; Chorionic gonadotropins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Reproductive Health (AREA)
  • Immunology (AREA)
  • Endocrinology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Methods are provided for the establishment and maintenance in long term culture of hormone secreting cells. Cells are derived from tumorous or non-tumorous animal or human tissues, including ovary, endometrium, trophoblast, pituitary, thyroid, and pancreas. The cells secrete into the culture medium hormones such as estrogens, progestins, follicle-stimulating hormone, luteinizing hormone, human chorionic gonadotrophin, thyroxin, glucagon, and insulin, depending on the tissue of origin of individual cell cultures. Contact with an appropriate secretogogue causes the cells to respond with increased hormone secretion. For instance, ovarian follicular cells respond to follicle-stimulating hormone with increased estrogen and progesterone secretion. Pancreatic cells respond to elevated glucose with increased insulin secretion. The cells proliferate in in vitro for up to one year or longer, during which time they retain their hormone-secretion profile. The cells may be frozen for storage, and retain their hormone-secretion profile after thawing. The cell cultures are useful for the production of human hormones, for the bio-assay of drugs such as therapeutic gonadotrophin, for the testing of drug efficacy and design, and for toxicity testing of drugs and chemicals. The cells may also be implanted in an individual to replace deficient hormone secretion. For instance, insulin secreting pancreatic cells may be implanted in a diabetic individual as an adjunct or replacement therapy for exogenously administered insulin.
DE69232535A 1991-06-24 1992-06-23 LONG-TERM CULTURE HORMONE-SECONDING PANCREATIC CELLS Expired - Fee Related DE69232535D1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71997791A 1991-06-24 1991-06-24
PCT/US1992/005267 WO1993000441A1 (en) 1991-06-24 1992-06-23 Hormone-secreting cells maintained in long-term culture

Publications (1)

Publication Number Publication Date
DE69232535D1 true DE69232535D1 (en) 2002-05-08

Family

ID=24892163

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69232535A Expired - Fee Related DE69232535D1 (en) 1991-06-24 1992-06-23 LONG-TERM CULTURE HORMONE-SECONDING PANCREATIC CELLS
DE69232535T Expired - Lifetime DE69232535T4 (en) 1991-06-24 1992-06-23 LONG-TERM CULTURE HORMONE-SEPARATING PANCREATIC CELLS

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69232535T Expired - Lifetime DE69232535T4 (en) 1991-06-24 1992-06-23 LONG-TERM CULTURE HORMONE-SEPARATING PANCREATIC CELLS

Country Status (10)

Country Link
US (2) US5928942A (en)
EP (1) EP0605428B9 (en)
JP (1) JPH06508527A (en)
AT (1) ATE215605T1 (en)
AU (2) AU2228592A (en)
CA (1) CA2112112A1 (en)
DE (2) DE69232535D1 (en)
DK (1) DK0605428T5 (en)
ES (1) ES2179821T3 (en)
WO (1) WO1993000441A1 (en)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6231881B1 (en) 1992-02-24 2001-05-15 Anton-Lewis Usala Medium and matrix for long-term proliferation of cells
US6352707B1 (en) 1992-02-24 2002-03-05 Anton-Lewis Usala Transplant encapsulation in a hydrogel matrix to obscure immune recognition
US6008047A (en) * 1993-04-08 1999-12-28 Livercell L.L.C. Cell culturing method and medium
JP2813467B2 (en) * 1993-04-08 1998-10-22 ヒューマン・セル・カルチャーズ・インコーポレーテッド Cell culture methods and media
US5534404A (en) * 1993-12-10 1996-07-09 Cytotherapeutics, Inc. Glucose responsive insulin secreting β-cell lines and method for producing same
US5834308A (en) * 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
US6703017B1 (en) 1994-04-28 2004-03-09 Ixion Biotechnology, Inc. Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US6001647A (en) * 1994-04-28 1999-12-14 Ixion Biotechnology, Inc. In vitro growth of functional islets of Langerhans and in vivo uses thereof
US6485723B1 (en) 1995-02-10 2002-11-26 Purdue Research Foundation Enhanced submucosal tissue graft constructs
US5695998A (en) * 1995-02-10 1997-12-09 Purdue Research Foundation Submucosa as a growth substrate for islet cells
AU739771B2 (en) * 1995-10-25 2001-10-18 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of langerhans and in vivo uses thereof
US6043092A (en) * 1996-03-18 2000-03-28 University Of Pittsburgh Cell culture media for mammalian cells
FR2757371B1 (en) * 1996-12-20 1999-03-26 Mxm ELASTIC DEVICE WITH LARGE ELONGATION CAPACITY FOR LIVE TISSUE EXTENSION
KR20000070879A (en) 1997-02-06 2000-11-25 브루스 엠. 에이센, 토마스 제이 데스로저 Human sdf-5 protein and compositions
US6165748A (en) 1997-07-11 2000-12-26 Genetics Institute, Inc. Frazzled nucleotide sequences and expression products
US6074874A (en) * 1997-08-29 2000-06-13 University Of Pittsburgh Epithelial cell cultures for in vitro testing
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6866843B2 (en) * 1999-12-06 2005-03-15 Viacell, Inc. Method of transplanting in a mammal and treating diabetes mellitus by administering a pseudo-islet like aggregate differentiated from a nestin-positive pancreatic stem cell
US20050042749A1 (en) 2001-05-16 2005-02-24 Carpenter Melissa K. Dopaminergic neurons and proliferation-competent precursor cells for treating Parkinson's disease
WO2001088104A2 (en) 2000-05-17 2001-11-22 Geron Corporation Neural progenitor cell populations
US6759039B2 (en) * 2000-06-30 2004-07-06 Amcyte, Inc. Culturing pancreatic stem cells having a specified, intermediate stage of development
AU2001273108A1 (en) * 2000-06-30 2002-01-14 Amcyte, Inc. Culturing pancreatic stem cells having a specified, intermediate stage of development
US6468989B1 (en) * 2000-07-13 2002-10-22 Dow Pharmaceutical Sciences Gel compositions containing metronidazole
US7202080B2 (en) 2001-03-29 2007-04-10 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic differentiation pathway
US6642003B2 (en) 2001-08-02 2003-11-04 Cedars-Sinai Medical Center Human glucose-dependent insulin-secreting cell line
GB2415432B (en) * 2001-12-07 2006-09-06 Geron Corp Islet cells from human embryonic stem cells
US7101546B2 (en) * 2001-12-21 2006-09-05 Amcyte, Inc. In situ maturation of cultured pancreatic stem cells having a specified, intermediate stage of development
US6881726B2 (en) * 2001-12-24 2005-04-19 Dow Pharmaceutical Sciences Aqueous compositions containing metronidazole
US7141240B2 (en) * 2002-03-12 2006-11-28 Cedars-Sinai Medical Center Glucose-dependent insulin-secreting cells transfected with a nucleotide sequence encoding GLP-1
US20050025838A1 (en) 2003-06-25 2005-02-03 Badylak Stephen F. Conditioned compositions for tissue restoration
JPWO2005110394A1 (en) * 2004-05-19 2008-03-21 味の素株式会社 Diabetes medicine
CN100446051C (en) * 2006-05-19 2008-12-24 刘芳 Method for building external nidation model of embryo body and usage
WO2007149182A2 (en) 2006-06-19 2007-12-27 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
US8652500B2 (en) 2009-07-22 2014-02-18 Acell, Inc. Particulate tissue graft with components of differing density and methods of making and using the same
US8298586B2 (en) 2009-07-22 2012-10-30 Acell Inc Variable density tissue graft composition
DK2493922T3 (en) 2009-10-26 2017-04-24 Hoffmann La Roche Process for preparing a glycosylated immunoglobulin
US9878071B2 (en) 2013-10-16 2018-01-30 Purdue Research Foundation Collagen compositions and methods of use
WO2016172365A1 (en) 2015-04-21 2016-10-27 Purdue Research Foundation Office Of Technology Commercialization Cell-collagen-silica composites and methods of making and using the same
US20180140640A1 (en) * 2015-04-22 2018-05-24 Cadens Beheer B.V. Improved contraceptive methods and compositions and devices for use therein
CN106701659A (en) * 2016-10-31 2017-05-24 四川农业大学 Porcine ovarian granulosa cell primary culture method
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
CA3061428A1 (en) 2017-04-25 2018-11-01 Purdue Research Foundation 3-dimensional (3d) tissue-engineered muscle for tissue restoration
CN112048540B (en) * 2020-09-14 2022-10-11 北京大学 Method for detecting insulin secretion in pancreatic islet and application thereof
CN113398251B (en) * 2021-07-30 2022-08-05 宁波人健药业集团股份有限公司 Recombinant human chorionic gonadotropin freeze-dried powder injection and preparation method thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3882233A (en) * 1973-07-27 1975-05-06 American Home Prod Methods of increasing production of pituitary hormones
US4288546A (en) * 1976-04-09 1981-09-08 The Regents Of The University Of Minnesota Process for the large scale production of pituitary hormones by serial secondary suspension culture
US4439521A (en) * 1981-10-21 1984-03-27 Ontario Cancer Institute Method for producing self-reproducing mammalian pancreatic islet-like structures
US4657866A (en) * 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
DK402984D0 (en) * 1984-08-23 1984-08-23 Nordisk Insulinlab PROCEDURE FOR PROMOTING INSULIN-PRODUCING CELLS
AU7281787A (en) * 1986-04-01 1987-10-20 Genelabs Incorporated Immortalized cells which produce tissue-specific products
DE3871206D1 (en) * 1987-03-24 1992-06-25 Grace W R & Co BASIC MEDIUM FOR A CELL CULTURE.
US4929542A (en) * 1987-10-30 1990-05-29 Cornell Research Foundation, Inc. In vitro screening test for mutagenicity and genotoxicity during spermatogenesis
US4980281A (en) * 1988-02-10 1990-12-25 Housey Gerard M Method of screening for protein inhibitors and activators
EP0363125A3 (en) * 1988-10-03 1990-08-16 Hana Biologics Inc. Proliferated pancreatic endocrine cell product and process
US4983515A (en) * 1989-02-16 1991-01-08 E. I. Du Pont De Nemours And Company Labeled cryopreserved cells for use as targets in cytotoxicity assays
US4983527A (en) * 1989-05-26 1991-01-08 Arizona Board Of Regents Oocyte test for detection of tumor promoting compounds
US5328844A (en) * 1990-05-09 1994-07-12 University Of Colorado Foundation, Inc. Culture media for mammalian cells
JP2813467B2 (en) * 1993-04-08 1998-10-22 ヒューマン・セル・カルチャーズ・インコーポレーテッド Cell culture methods and media
US5534404A (en) * 1993-12-10 1996-07-09 Cytotherapeutics, Inc. Glucose responsive insulin secreting β-cell lines and method for producing same

Also Published As

Publication number Publication date
EP0605428B9 (en) 2003-01-02
EP0605428A1 (en) 1994-07-13
WO1993000441A1 (en) 1993-01-07
CA2112112A1 (en) 1993-01-07
US6372493B1 (en) 2002-04-16
DK0605428T5 (en) 2003-01-06
JPH06508527A (en) 1994-09-29
DE69232535T2 (en) 2003-01-09
ATE215605T1 (en) 2002-04-15
AU2228592A (en) 1993-01-25
EP0605428A4 (en) 1995-01-11
ES2179821T3 (en) 2003-02-01
AU700946B2 (en) 1999-01-14
AU7036596A (en) 1997-02-20
DK0605428T3 (en) 2002-07-29
DE69232535T4 (en) 2003-09-11
US5928942A (en) 1999-07-27
EP0605428B1 (en) 2002-04-03

Similar Documents

Publication Publication Date Title
DE69232535D1 (en) LONG-TERM CULTURE HORMONE-SECONDING PANCREATIC CELLS
Herbert Localization of antisera to LHβ and FSHβ in the rat pituitary gland
Dozortsev et al. Luteinizing hormone–independent rise of progesterone as the physiological trigger of the ovulatory gonadotropins surge in the human
ATE553766T1 (en) PREPARATION AND XENOTRANSPLANTATION OF ISLANDS OF LANGERHANS FROM PIGS
NO320713B1 (en) Pharmaceutical preparation comprising Sertoli cells and cells producing a biological factor, sectioned set of containers for the cells, product article comprising a packaging material with Sertoli cells and methods for increasing the recovery and profiling of cells ex vivo.
Fölsch et al. Effect of somatostatin on basal and stimulated pancreatic secretion in the rat
Roser et al. Superovulation in the mare: a work in progress
Hammond et al. Local maintenance of the rabbit corpus luteum with oestrogen
WEICK et al. Localization of the stimulatory feedback effect of estrogen on ovulation in the rat
Van Oordt et al. Seasonal changes in endocrine organs of the male common frog, Rana temporaria: I. The pars distalis of the adenohypophysis
Korsgren et al. Glucose effectiveness: the mouse trap in the development of novel ss-cell replacement therapies
McCracken The role of prostaglandins in luteal regression
Bosu et al. Effect of HCG on plasma levels of estrogens and progesterone during the luteal phase of the menstrual cycle in rhesus monkeys (Macaca mulatta). Stimulation of sex steroid production in rhesus monkeys
Kuzuya et al. Studies on the mechanism of xylitol-induced insulin secretion in dogs. Effect of its infusion into the pancreatic artery, and the inhibition by epinephrine and diazoxide of xylitol-induced hyperinsulinaemia
Moses et al. Dissociation between the content of vasopressin and neurosecretory material in the rat neurohypophysis
Iyer et al. Delta sleep inducing peptide (DSIP) stimulates the release of LH but not FSH via a hypothalamic site of action in the rat
Rennie Luteal-hypophyseal interrelationship in the rabbit
CHOUDARY et al. Luteolytic effect of oestrogen on the corpora lutea of the cyclic guinea-pig
De Castro et al. Possible negative ultra-short loop feedback of luteinizing hormone releasing hormone (LHRH) in the ovariectomized rat
Gemmell et al. Granule secretion by the luteal cell of the sheep: the fate of the granule membrane
Piacsek et al. Stimulation by light of gonadotropin release from transplanted pituitaries of hypophysectomized rats
GREENWALD Dissociation of Ovulation and Progesterone Secretion Following LH or FSH Treatment of Hypophysectomized Prbestrous Hamsters
Schatz et al. Hypophysis and function of pancreatic islets. 3. Secretion and biosynthesis of insulin in isolated pancreatic islets of intact and hypophysectomized rats in the presence of growth hormone, corticotrophin and human chorionic somatotrophin in vitro
Foster Jr et al. On the secretion of corticotrophin by the homotransplanted pituitary gland in the rat
Kullander Experimental studies in the production of androgen in ovarian tissue autotransplanted to the spleen of spayed rats

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: HCELL TECHNOLOGY, INC., RENO, NV, US

8328 Change in the person/name/address of the agent

Free format text: V. FUENER EBBINGHAUS FINCK HANO, 81541 MUENCHEN

8339 Ceased/non-payment of the annual fee