DE614707C - Process for the production of shaped, precisely dosed organ protein preparations - Google Patents
Process for the production of shaped, precisely dosed organ protein preparationsInfo
- Publication number
- DE614707C DE614707C DEC45872D DEC0045872D DE614707C DE 614707 C DE614707 C DE 614707C DE C45872 D DEC45872 D DE C45872D DE C0045872 D DEC0045872 D DE C0045872D DE 614707 C DE614707 C DE 614707C
- Authority
- DE
- Germany
- Prior art keywords
- protein preparations
- shaped
- production
- organ
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/146—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/143—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with inorganic compounds
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Inorganic Chemistry (AREA)
- Medicinal Preparation (AREA)
Description
Verfahren zur Herstellung von geformten, genau dosierten Organeiweißpräparaten Die zur Feststellung von spezifischen Organeiweißabbaufermenten im Serum erforderlichen Mengen an Organeiweißpräparaten sind so gering, daß durch Abwägen, selbst bei Anwendung besonderer Vorsichtsmaßregeln, nur ungenaue Ergebnisse erzielt werden. Stichproben, die mit den Präparaten in handelsüblicher Fassung vorgenommen wurden, ergaben bei 18 Wägungen eine Schwankung zwischen 3,4 und 6,8 mg.Process for the production of shaped, precisely dosed organ protein preparations The necessary to determine specific organ protein degradation fermentations in the serum Quantities of organ protein preparations are so small that by weighing them up, even when using them special precautions, only inaccurate results will be achieved. Samples, which were carried out with the preparations in the commercially available version resulted in 18 weighings a fluctuation between 3.4 and 6.8 mg.
Bei Überführung der Präparate aus den Röhrchen in das Probeglas bleibt ein verhältnismäßig großer Anteil an den Verpackungsröhrchen haften.When the specimens are transferred from the tubes into the test glass, this remains a relatively large proportion adhere to the packaging tube.
Des ferneren bieten die in der bisherigen Form gelieferten Eiweißpräparate dem Angriff des Serums eine nur kleine Oberfläche dar, da sich die einzelnen, verhältnismäßig großen Teilchen des pulverförmigen Präparates rasch in dicker Schicht auf dem Boden des Röhrchens absetzen.Furthermore, offer the protein preparations supplied in the previous form the attack of the serum represents only a small surface, since the individual, proportionally large particles of the powdery preparation quickly in a thick layer on the floor of the tube.
Weiterhin verbürgt die neue Formgebung der Organsubstrate im Gegensatz zu der bisher üblichen Form ein sicher steriles und rasches Arbeiten.Furthermore, the new shape of the organ substrates vouches for the contrary safe, sterile and quick work in addition to the usual form.
Schließlich werden beim Umfüllen der Serumflüssigkeit in das Untersuchungsrohr Teilchen des Substrates mitgerissen, wodurch die Messung gestört wird.Finally, when transferring the serum fluid into the examination tube Particles of the substrate are carried away, which disrupts the measurement.
Die vorliegende Erfindung beseitigt die erwähnten Mängel, führt zu einer Verminderung der für die Untersuchung erforderlichen Substratmengen gegenüber den in Röhrchen abgefaßten und verkürzt ,die notwendige Einwirkungszeit der Enzyme auf die Substrate.The present invention overcomes the deficiencies mentioned, leads to compared to a reduction in the amount of substrate required for the investigation those in tubes and shortened, the necessary time for the enzymes to act on the substrates.
Zu diesem Zwecke werden die Eiweißpräparate in möglichst feiner Verteilung auf Trägermassen niedergeschlagen oder in sie eingebettet. Die Trägermassen müssen dem Serum den ungehinderten Zutritt zu den Eiweißpräparaten gestatten, eine gleichmäßige Verteilung und eine große Oberfläche der Eiweißteilchen gewährleisten, dürfen an das Serum keinerlei Substanzen abgeben und dürfen das .Serum weder angreifen noch verändern; sie dürfen auch- keine Bestandteile des Serums übernehmen, absorbieren, zerstören o. dgl.For this purpose, the protein preparations are distributed as finely as possible deposited on or embedded in carrier masses. The carrier masses must allow the serum unhindered access to the protein preparations, an even Distribution and a large surface area of the protein particles are allowed to the serum does not release any substances and must neither attack nor attack the serum change; they must not take over or absorb any components of the serum, destroy or the like
Als geeignet erweisen sich faserige Stoffe, wie Cellulosefasern, Baumwolle, Filterpapierfasern, Asbest, Kunstfasern, besonders präparierte Erden, die im Blindversuch darauf geprüft werden, ob sie das Serum in irgendeiner Weise beeinflussen.Fibrous materials such as cellulose fibers, cotton, Filter paper fibers, asbestos, synthetic fibers, specially prepared soils that are used in the blind test checked to see if they affect the serum in any way.
Sodann werden die getrockneten Organeiweißpräparate nach vorheriger äußerst feiner Zerkleinerung mit dem Trägermaterial innig vermischt, wobei Flüssigkeiten, wie Aceton, Anwendung finden können. Aus der einheitlich zusammengesetzten Mischung werden nach sorgfältiger Trocknung maschinell Blättchen ausgestanzt oder Tabletten geformt. Hierdurch wird eine gleichmäßige und saubere, exakte Dosierung der Eiweißsubstrate unter großer Zeit- und Arbeitsersparnis erzielt.Then the dried organ protein preparations after previous extremely fine comminution intimately mixed with the carrier material, whereby liquids, such as acetone, can be used. From the uniformly composed mixture After careful drying, leaves or tablets are machine punched out shaped. This is a uniform and clean, exact dosage of protein substrates achieved with great time and labor savings.
Die Anwendung der so vorbereiteten Eiweißpräparate bei der Untersuchung des Serums erfordert nunmehr eine geringe Mühe, da die Blättchen oder Tabletten, ohne daß ein Substanzverlust zu befürchten wäre, in das Röhrchen eingeführt und mit dem Serum in Reaktion gebracht werden.The use of the protein preparations prepared in this way during the examination of the serum now requires little effort, as the leaflets or tablets, without fear of loss of substance, inserted into the tube and reacted with the serum.
Folgendes Beispiel diene zur Veranschaulichung des Verfahrens: Schlachtfrische Rinderschilddrüsen, welche von allen fremden Gewebeteilen, z. B. Bindegewebe, befreit sind, werden zu einem Brei zerkleinert und beispielsweise in einem Filter mit einprozentiger wäßriger Kochsalzlösung so lange ;gewaschen, bis alle löslichen Stoffe aus der Drüsensubstanz entfernt sind. Durch Vergleich der Waschflüssigkeit vor und nach der Waschung mittels des Interferometers kann leicht erkannt werden, wann das Organ von allen löslichen Stoffen befreit ist. Mittels Dialyse gegen destilliertes Wasser wird hierauf das Natriumchlorid aus dem Organbrei entfernt. Die restlose Entfernung des Kochsalzes wird ebenfalls interferometrisch kontrolliert. Die so gereinigte Drüse wird nun entweder im Hochvakuum bei Temperaturen nicht über 36° oder durch Anwendung von wasserfreiem Aceton getrocknet und dann, beispielsweise in einer Kugelmühle oder einer Kolloidmühle, weitgehend zerkleinert.The following example serves to illustrate the process: slaughter freshness Bovine thyroid glands, which are separated from all foreign tissue parts, e.g. B. connective tissue, exempted are crushed to a pulp and, for example, in a filter with one percent aqueous saline solution so long; washed until all soluble substances from the glandular substance are away. By comparing the washing liquid before and after washing by means of the interferometer can easily be seen when the organ is soluble from all Substances is exempt. Using dialysis against distilled water, the Sodium chloride removed from the organ pulp. The complete removal of the table salt is also checked interferometrically. The gland thus cleaned is now either in a high vacuum at temperatures not exceeding 36 ° or by using dried anhydrous acetone and then, for example in a ball mill or a colloid mill, largely crushed.
Das mehlfeine Pulver, das eine Teilchengröße von o,oi bis o,i mm im Durchmesser haben soll, wird nun mit gut gereinigten Filterpapierfasern, welche erfahrungsgemäß vom Serum nicht angegriffen werden, in wasserfreiem Aceton zu einem gleichmäßigen Brei angerührt. Auf einem Büchner-Trichter oder einer ähnlichen Vorrichtung wird das Gemisch abgesaugt, wodurch eine papierartige Schicht auf der Trichterplatte zurückbleibt. Diese papierähnliche Masse enthält das Organpulver zwischen den Filterpapierfasern in gleichmäßiger, äußerst feiner Verteilung eingebettet. Nach dem Trocknen werden mit einer geeigneten Maschine oder von Hand aus der papierartigen Masse Blättchen von geeigneter Größe ausgestanzt.The flour-fine powder, which has a particle size of o, oi to o, i mm in Should have diameter, is now with well cleaned filter paper fibers, which experience has shown that the serum does not attack them, in anhydrous acetone to one evenly mixed pulp. On a Büchner funnel or similar device the mixture is suctioned off, creating a paper-like layer on the funnel plate remains behind. This paper-like mass contains the organ powder between the filter paper fibers embedded in an even, extremely fine distribution. After drying will be with a suitable machine or by hand from the paper-like mass flakes punched out of suitable size.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEC45872D DE614707C (en) | 1932-01-09 | 1932-01-09 | Process for the production of shaped, precisely dosed organ protein preparations |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEC45872D DE614707C (en) | 1932-01-09 | 1932-01-09 | Process for the production of shaped, precisely dosed organ protein preparations |
Publications (1)
Publication Number | Publication Date |
---|---|
DE614707C true DE614707C (en) | 1935-06-17 |
Family
ID=7026206
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DEC45872D Expired DE614707C (en) | 1932-01-09 | 1932-01-09 | Process for the production of shaped, precisely dosed organ protein preparations |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE614707C (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1012432B (en) * | 1954-07-29 | 1957-07-18 | Josef Ellendorf & Co Dr Med | Process for the production of durable, phenol-containing molded preparations for the internal treatment of skin diseases, especially psoriasis |
-
1932
- 1932-01-09 DE DEC45872D patent/DE614707C/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1012432B (en) * | 1954-07-29 | 1957-07-18 | Josef Ellendorf & Co Dr Med | Process for the production of durable, phenol-containing molded preparations for the internal treatment of skin diseases, especially psoriasis |
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