DE602006018625D1 - Marker-freie pflanzentransformation - Google Patents

Marker-freie pflanzentransformation

Info

Publication number
DE602006018625D1
DE602006018625D1 DE602006018625T DE602006018625T DE602006018625D1 DE 602006018625 D1 DE602006018625 D1 DE 602006018625D1 DE 602006018625 T DE602006018625 T DE 602006018625T DE 602006018625 T DE602006018625 T DE 602006018625T DE 602006018625 D1 DE602006018625 D1 DE 602006018625D1
Authority
DE
Germany
Prior art keywords
plant transformation
marker
free plant
seedling
vortexing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006018625T
Other languages
English (en)
Inventor
Caius Rommens
Troy J Weeks
Jingsong Ye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
JR Simplot Co
Original Assignee
JR Simplot Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by JR Simplot Co filed Critical JR Simplot Co
Publication of DE602006018625D1 publication Critical patent/DE602006018625D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
DE602006018625T 2005-07-27 2006-07-26 Marker-freie pflanzentransformation Active DE602006018625D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70263805P 2005-07-27 2005-07-27
PCT/US2006/029246 WO2007016276A2 (en) 2005-07-27 2006-07-26 Marker-free plant transformation

Publications (1)

Publication Number Publication Date
DE602006018625D1 true DE602006018625D1 (de) 2011-01-13

Family

ID=37668175

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006018625T Active DE602006018625D1 (de) 2005-07-27 2006-07-26 Marker-freie pflanzentransformation

Country Status (6)

Country Link
US (1) US7449335B2 (de)
EP (1) EP1915453B1 (de)
AT (1) ATE490330T1 (de)
DE (1) DE602006018625D1 (de)
ES (1) ES2355062T3 (de)
WO (1) WO2007016276A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101385051B1 (ko) 2006-02-09 2014-04-24 메디카고 인코포레이티드 식물에서 시알산의 합성
KR20100031603A (ko) * 2007-06-15 2010-03-23 메디카고 인코포레이티드 식물에서의 단백질 생산
US9045759B2 (en) * 2009-01-21 2015-06-02 James Arthur Williams DNA plasmids with improved copy number
RU2731295C2 (ru) 2014-07-11 2020-09-01 Медикаго Инк. Модификация продуцирования белков в растениях
CN112501211B (zh) * 2020-12-21 2022-11-01 贵州大学 一种利用注射遗传转化杜仲的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0267159A3 (de) 1986-11-07 1990-05-02 Ciba-Geigy Ag Verfahren zur genetischen Modifikation monokotyler Pflanzen
US5286635A (en) * 1991-09-24 1994-02-15 Freshworld, L.P. Genetically transformed pea plants and methods for their production
US5948956A (en) * 1997-10-16 1999-09-07 Oms Investments, Inc. Transgenic plants and method for node segment transformation
FR2791358B1 (fr) * 1999-03-22 2003-05-16 Meristem Therapeutics Promoteurs chimeriques d'expression, cassettes d'expression, plasmides, vecteurs, plantes et semences transgeniques les contenant et leurs methodes d'obtention
BRPI0014481B1 (pt) * 1999-10-04 2015-12-08 Medicago Inc construto e uso do mesmo para regular a expressão de um gene exógeno em uma planta transgênica
WO2002036786A2 (en) * 2000-10-31 2002-05-10 Medicago Inc. Method of selecting plant promoters to control transgene expression
WO2003069980A2 (en) 2002-02-20 2003-08-28 J.R. Simplot Company Precise breeding
ATE509111T1 (de) * 2002-03-20 2011-05-15 Simplot Co J R Verfeinerte pflanzentransformation
US20040016015A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system

Also Published As

Publication number Publication date
EP1915453B1 (de) 2010-12-01
EP1915453A2 (de) 2008-04-30
ATE490330T1 (de) 2010-12-15
US20070033678A1 (en) 2007-02-08
US7449335B2 (en) 2008-11-11
WO2007016276A2 (en) 2007-02-08
ES2355062T3 (es) 2011-03-22
WO2007016276A3 (en) 2008-04-17

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